(Circular) (Six-base) MAPSORT of: pFN21AB0475.seq Check: 8412 from: 1 to: 6256 pFN21AB0475 6256 bp With 182 enzymes: SgfI * April 15, 2010 09:33 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2525 2816 2525 Size: 291 5965 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4066 278 Size: 53 83 186 936 2530 2468 Fragments arranged by size: 2530 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2367 3599 1937 Size: 430 1232 4594 Fragments arranged by size: 4594 1232 430 Acc65I G'GTAC_C Cuts at: 2459 2778 3577 2459 Size: 319 799 5138 Fragments arranged by size: 5138 799 319 AclI AA'CG_TT Cuts at: 1563 4383 4756 1563 Size: 2820 373 3063 Fragments arranged by size: 3063 2820 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4319 5703 5971 1632 Size: 2687 1384 268 1917 Fragments arranged by size: 2687 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6059 AflIII A'CryG_T Cuts at: 1203 3186 5155 6132 1203 Size: 1983 1969 977 1327 Fragments arranged by size: 1983 1969 1327 977 AgeI A'CCGG_T Cuts at: 5087 5087 Size: 6256 AhdI GACnn_n'nnGTC Cuts at: 4985 4985 Size: 6256 AleI CACnn'nnGTG Cuts at: 1166 2141 1166 Size: 975 5281 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6256 AlwNI CAG_nnn'CTG Cuts at: 2837 3250 3999 5571 2837 Size: 413 749 1572 3522 Fragments arranged by size: 3522 1572 749 413 ApaLI G'TGCA_C Cuts at: 4313 5469 4313 Size: 1156 5100 ApoI r'AATT_y Cuts at: 1493 2297 3008 3565 3791 3943 6052 1493 Size: 804 711 557 226 152 2109 1697 Fragments arranged by size: 2109 1697 804 711 557 226 152 AscI GG'CGCG_CC Cuts at: 3046 3046 Size: 6256 AseI AT'TA_AT Cuts at: 160 1032 4810 160 Size: 872 3778 1606 Fragments arranged by size: 3778 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6256 AvaI C'yCGr_G Cuts at: 1870 1943 3581 1870 Size: 73 1638 4545 Fragments arranged by size: 4545 1638 73 BamHI G'GATC_C Cuts at: 3586 3953 3586 Size: 367 5889 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2027 2459 2644 2778 3058 Size: 325 217 627 240 432 185 134 280 Cuts at: 3058 3144 3577 5032 618 Size: 86 433 1455 1842 Fragments arranged by size: 1842 1455 627 433 432 325 280 240 217 185 134 86 BanII G_rGCy'C Cuts at: 1391 3575 1391 Size: 2184 4072 BbeI G_GCGC'C Cuts at: 2031 3148 2031 Size: 1117 5139 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5285 BbvCI CC'TCA_GC Cuts at: 2524 2524 Size: 6256 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4447 4481 1903 Size: 34 2510 34 3678 Fragments arranged by size: 3678 2510 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2563 4148 5358 2563 Size: 1585 1210 3461 Fragments arranged by size: 3461 1585 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6256 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4867 136 Size: 107 122 71 631 605 3195 1525 Fragments arranged by size: 3195 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6251 6251 Size: 6256 BlpI GC'TnA_GC Cuts at: 3673 3673 Size: 6256 Bme1580I G_kGCm'C Cuts at: 1790 2649 3063 4317 5473 1790 Size: 859 414 1254 1156 2573 Fragments arranged by size: 2573 1254 1156 859 414 BmgBI CAC'GTC Cuts at: 1318 2143 2797 1318 Size: 825 654 4777 Fragments arranged by size: 4777 825 654 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2538 3305 3987 Size: 416 53 83 399 24 1121 767 682 Cuts at: 3987 4945 442 Size: 958 1753 Fragments arranged by size: 1753 1121 958 767 682 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6256 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2366 2372 4916 1239 Size: 1127 6 2544 2579 Fragments arranged by size: 2579 2544 1127 6 Bpu10I CC'TnA_GC Cuts at: 2524 2980 3480 2524 Size: 456 500 5300 Fragments arranged by size: 5300 500 456 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4381 5246 5544 4381 Size: 865 298 5093 Fragments arranged by size: 5093 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4919 882 Size: 617 3420 2219 Fragments arranged by size: 3420 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3187 3553 4047 493 Size: 711 1983 366 494 2702 Fragments arranged by size: 2702 1983 711 494 366 BsaBI GATnn'nnATC Cuts at: 6250 6250 Size: 6256 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2028 2085 3145 3393 Size: 53 83 186 936 495 57 1060 248 Cuts at: 3393 4063 4445 5080 275 Size: 670 382 635 1451 Fragments arranged by size: 1451 1060 936 670 635 495 382 248 186 83 57 53 BsaWI w'CCGG_w Cuts at: 2598 3453 4689 5087 5361 5508 5919 2598 Size: 855 1236 398 274 147 411 2935 Fragments arranged by size: 2935 1236 855 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3010 3040 1178 Size: 30 165 30 1607 30 4394 Fragments arranged by size: 4394 1607 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3018 3329 3332 3335 3338 3341 3344 3347 Size: 1832 311 3 3 3 3 3 3 Cuts at: 3347 1186 Size: 4095 Fragments arranged by size: 4095 1832 311 3 3 3 3 3 3 BseYI C'CCAG_C Cuts at: 2177 2648 2830 3222 5459 5955 2177 Size: 471 182 392 2237 496 2478 Fragments arranged by size: 2478 2237 496 471 392 182 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2135 2503 2753 1163 Size: 573 399 368 250 4666 Fragments arranged by size: 4666 573 399 368 250 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3706 4467 5495 1540 Size: 275 186 1705 761 1028 2301 Fragments arranged by size: 2301 1705 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4317 4402 5473 4317 Size: 85 1071 5100 Fragments arranged by size: 5100 1071 85 BsmI GAATG_Cn' Cuts at: 2069 3767 3860 2069 Size: 1698 93 4465 Fragments arranged by size: 4465 1698 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3030 3030 Size: 6256 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2649 3063 3575 4317 4402 5473 1391 Size: 399 859 414 512 742 85 1071 2174 Fragments arranged by size: 2174 1071 859 742 512 414 399 85 BspEI T'CCGG_A Cuts at: 2598 5919 2598 Size: 3321 2935 BspHI T'CATG_A Cuts at: 4145 5875 4145 Size: 1730 4526 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2525 2790 2816 3258 3611 844 Size: 1681 265 26 442 353 3489 Fragments arranged by size: 3489 1681 442 353 265 26 BsrBI CCG'CTC Cuts at: 1394 1823 4143 1394 Size: 429 2320 3507 Fragments arranged by size: 3507 2320 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4751 4925 65 Size: 1183 3503 174 1396 Fragments arranged by size: 3503 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3055 3147 3287 4900 5087 1700 Size: 110 1245 92 140 1613 187 2869 Fragments arranged by size: 2869 1613 1245 187 140 110 92 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6256 BssHII G'CGCG_C Cuts at: 1925 3046 1925 Size: 1121 5135 BssSI C'ACGA_G Cuts at: 4316 5328 4316 Size: 1012 5244 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6256 BstBI TT'CG_AA Cuts at: 5060 5060 Size: 6256 BstEII G'GTnAC_C Cuts at: 1184 2749 1184 Size: 1565 4691 BstXI CCAn_nnnn'nTGG Cuts at: 2881 2881 Size: 6256 BstYI r'GATC_y Cuts at: 1972 2595 3586 3953 4338 4355 5807 5893 5905 Size: 623 991 367 385 17 1452 86 12 Cuts at: 5905 6251 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 991 623 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2745 2745 Size: 6256 BtgI C'CryG_G Cuts at: 513 1065 2004 2256 3063 3414 513 Size: 552 939 252 807 351 3355 Fragments arranged by size: 3355 939 807 552 351 252 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5517 Fragments arranged by size: 5517 683 56 BtsI GCAGTG_nn' Cuts at: 2152 2475 3772 4566 4586 2152 Size: 323 1297 794 20 3822 Fragments arranged by size: 3822 1297 794 323 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6256 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6221 DraI TTT'AAA Cuts at: 3560 3560 Size: 6256 DraIII CAC_nnn'GTG Cuts at: 3299 3299 Size: 6256 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5263 809 Size: 507 3947 1802 Fragments arranged by size: 3947 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2238 2433 2511 Size: 54 1425 50 275 18 408 195 78 Cuts at: 2511 3066 3703 4592 6221 8 Size: 555 637 889 1629 43 Fragments arranged by size: 1629 1425 889 637 555 408 275 195 78 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3703 1537 Size: 275 1891 4090 Fragments arranged by size: 4090 1891 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2909 4186 1353 Size: 1556 1277 3423 Fragments arranged by size: 3423 1556 1277 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2321 4843 5217 5363 387 Size: 1064 138 732 2522 374 146 1280 Fragments arranged by size: 2522 1280 1064 732 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2366 2372 4319 4916 5703 5971 1239 Size: 393 734 6 1947 597 787 268 1524 Fragments arranged by size: 1947 1524 787 734 597 393 268 6 EcoO109I rG'GnC_Cy Cuts at: 2503 2915 2940 2503 Size: 412 25 5819 Fragments arranged by size: 5819 412 25 EcoRI G'AATT_C Cuts at: 2297 3008 3565 3943 2297 Size: 711 557 378 4610 Fragments arranged by size: 4610 711 557 378 FseI GG_CCGG'CC Cuts at: 3291 3291 Size: 6256 FspI TGC'GCA Cuts at: 4762 4762 Size: 6256 HaeII r_GCGC'y Cuts at: 1678 2031 3148 5403 1678 Size: 353 1117 2255 2531 Fragments arranged by size: 2531 2255 1117 353 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2040 2072 4897 4929 4971 Size: 32 135 32 468 32 2825 32 42 Cuts at: 4971 5003 1373 Size: 32 2626 Fragments arranged by size: 2825 2626 468 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2689 3600 3846 669 Size: 988 281 751 911 246 3079 Fragments arranged by size: 3079 988 911 751 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6256 HpaI GTT'AAC Cuts at: 3846 3846 Size: 6256 KasI G'GCGC_C Cuts at: 2027 3144 2027 Size: 1117 5139 KpnI G_GTAC'C Cuts at: 2463 2782 3581 2463 Size: 319 799 5138 Fragments arranged by size: 5138 799 319 MfeI C'AATT_G Cuts at: 2446 3855 2446 Size: 1409 4847 MluI A'CGCG_T Cuts at: 6132 6132 Size: 6256 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5370 5554 1305 Size: 4065 184 2007 Fragments arranged by size: 4065 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2240 2435 2513 10 Size: 54 1425 343 408 195 78 3753 Fragments arranged by size: 3753 1425 408 343 195 78 54 MslI CAynn'nnrTG Cuts at: 518 1166 2141 2261 2429 3189 4214 4573 4732 Size: 648 975 120 168 760 1025 359 159 Cuts at: 4732 518 Size: 2042 Fragments arranged by size: 2042 1025 975 760 648 359 168 159 120 MspA1I CmG'CkG Cuts at: 1606 2033 2788 3487 3672 4349 5497 5742 1606 Size: 427 755 699 185 677 1148 245 2120 Fragments arranged by size: 2120 1148 755 699 677 427 245 185 NaeI GCC'GGC Cuts at: 3149 3289 3149 Size: 140 6116 NarI GG'CG_CC Cuts at: 2028 3145 2028 Size: 1117 5139 NcoI C'CATG_G Cuts at: 513 1065 2004 2256 513 Size: 552 939 252 4513 Fragments arranged by size: 4513 939 552 252 NdeI CA'TA_TG Cuts at: 387 387 Size: 6256 NgoMIV G'CCGG_C Cuts at: 3147 3287 3147 Size: 140 6116 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6256 NotI GC'GGCC_GC Cuts at: 3703 3703 Size: 6256 NspI r_CATG'y Cuts at: 1132 1229 3614 5159 1132 Size: 97 2385 1545 2229 Fragments arranged by size: 2385 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6256 PciI A'CATG_T Cuts at: 5155 5155 Size: 6256 PflMI CCAn_nnn'nTGG Cuts at: 1108 2448 3299 1108 Size: 1340 851 4065 Fragments arranged by size: 4065 1340 851 PmeI GTTT'AAAC Cuts at: 3560 3560 Size: 6256 PmlI CAC'GTG Cuts at: 3187 3187 Size: 6256 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4325 4357 5838 5870 4325 Size: 32 1481 32 4711 Fragments arranged by size: 4711 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2503 2915 2940 2503 Size: 412 25 5819 Fragments arranged by size: 5819 412 25 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6256 PsiI TTA'TAA Cuts at: 3826 3826 Size: 6256 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3239 3271 3239 Size: 32 6224 PstI C_TGCA'G Cuts at: 830 2159 2787 3255 3608 830 Size: 1329 628 468 353 3478 Fragments arranged by size: 3478 1329 628 468 353 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6256 PvuII CAG'CTG Cuts at: 3487 3487 Size: 6256 SalI G'TCGA_C Cuts at: 1936 3598 1936 Size: 1662 4594 SanDI GG'GwC_CC Cuts at: 2915 2915 Size: 6256 SbfI CC_TGCA'GG Cuts at: 3608 3608 Size: 6256 ScaI AGT'ACT Cuts at: 4504 6216 4504 Size: 1712 4544 SfcI C'TryA_G Cuts at: 826 1045 2155 2783 2926 3169 3202 3251 3604 Size: 219 1110 628 143 243 33 49 353 Cuts at: 3604 4739 5420 5611 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1135 1110 681 628 353 243 219 191 143 49 33 SfoI GGC'GCC Cuts at: 2029 3146 2029 Size: 1117 5139 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6256 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6256 SmaI CCC'GGG Cuts at: 1872 3583 1872 Size: 1711 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4360 5261 5523 820 Size: 197 926 2417 901 262 1553 Fragments arranged by size: 2417 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4047 493 Size: 3554 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6256 SphI G_CATG'C Cuts at: 1132 3614 1132 Size: 2482 3774 SspI AAT'ATT Cuts at: 5 52 4180 5 Size: 47 4128 2081 Fragments arranged by size: 4128 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6256 StyI C'CwwG_G Cuts at: 513 1065 2004 2256 2673 2874 3695 513 Size: 552 939 252 417 201 821 3074 Fragments arranged by size: 3074 939 821 552 417 252 201 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2207 3246 3287 4283 4300 4453 4638 Size: 1255 627 1039 41 996 17 153 185 Cuts at: 4638 5083 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1039 996 627 445 185 153 41 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4502 6214 96 Size: 274 80 33 51 1443 2525 1712 138 Fragments arranged by size: 2525 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2654 2686 3010 3042 6216 6248 2654 Size: 32 324 32 3174 32 2662 Fragments arranged by size: 3174 2662 324 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2501 1316 Size: 1185 5071 XbaI T'CTAG_A Cuts at: 3592 3592 Size: 6256 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2233 2011 Size: 222 6034 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6256 XmaI C'CCGG_G Cuts at: 1870 3581 1870 Size: 1711 4545 XmnI GAAnn'nnTTC Cuts at: 4385 4385 Size: 6256 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4064 276 Size: 53 83 186 936 2530 2468 Fragments arranged by size: 2530 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AvrII BaeI BfrBI BplI BsiWI BstZ17I EcoICRI EcoNI EcoRV FalI FspAI NruI NsiI PacI PfoI PspOMI PspXI RsrII SacI SacII SapI SexAI SfiI SrfI SwaI