Restriction map for
pFN21AB0473
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# NO CUT ENZYMES ( 117 enzymes) #
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AatII AccIII AclI AcsI AflII
AgeI AhdI AlwI AocI Aor51HI
ApaI ApoI AscI AseI AsnI
Asp700I AspEI AvrII BamHI BbeI
BbrPI BcgI BciVI BclI BfaI
BfrI BlnI BsaBI BsaWI BseAI
BsiEI BsiWI BsmBI BsmI Bsp1407I
BspEI BspHI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstBI BstZ17I
Bsu36I Cfr10I CpoI DraI DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoRI EcoRV EcoT22I FbaI
FseI HincII HindII HindIII HpaI
KasI KspI MaeI MamI MfeI
MluI MroI MseI MunI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SapI SbfI SexAI
SfoI SfuI SgrAI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI TfiI Tru9I Tsp509I XbaI
XhoI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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Acc65I 856 GGTACC G^GTACC
AcyI 48 GACGCC G[GA]^CG[CT]C
AflIII 296 ACATGT A^C[GA][CT]GT
Alw44I 517 GTGCAC G^TGCAC
ApaLI 517 GTGCAC G^TGCAC
Asp718I 856 GGTACC G^GTACC
AspI 307 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1217 TGCGCA TGC^GCA
BbsI 672 GTCTTC GAAGAC(2/6)
BbvCI 475 GCTGAGG CCTCAGC(-5/-2)
BglI 1402 GCCATCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1361 AGATCT A^GATCT
BpuAI 672 GTCTTC GAAGAC(2/6)
BsaHI 48 GACGCC G[GA]^CG[CT]C
BsaI 1051 GGTCTC GGTCTC(1/5)
BspDI 1243 ATCGAT AT^CGAT
BspLU11I 296 ACATGT A^CATGT
BsrBI 1105 GAGCGG CCGCTC(-3/-3)
BstEII 993 GGTCACC G^GT[ACGT]ACC
BstPI 993 GGTCACC G^GT[ACGT]ACC
BstXI 942 CCAGGCACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 1361 AGATCT [GA]^GATC[CT]
ClaI 1243 ATCGAT AT^CGAT
DraIII 1165 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 92 CCATGG C^C[GA][CT]GG
EarI 1081 GAAGAG CTCTTC(1/4)
EcoNI 69 CCTTCAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 993 GGTCACC G^GT[ACGT]ACC
FspI 1217 TGCGCA TGC^GCA
Hin1I 48 GACGCC G[GA]^CG[CT]C
KpnI 856 GGTACC GGTAC^C
Ksp632I 1081 GAAGAG CTCTTC(1/4)
MflI 1361 AGATCT [GA]^GATC[CT]
NcoI 92 CCATGG C^CATGG
NspI 296 ACATGT [GA]CATG^[CT]
PflFI 307 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 903 CCAGCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 875 GACTGGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
ScaI 704 AGTACT AGT^ACT
SfiI 1401 GGCCATCCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 593 CCCGGG CCC^GGG
Tth111I 307 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 903 CCAGCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 1361 AGATCT [GA]^GATC[CT]
XmaCI 593 CCCGGG C^CCGGG
XmaI 593 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AccI 211 GTCTAC GT^[AC][GT]AC
1200 GTCTAC
BanI 856 GGTACC G^G[CT][GA]CC
945 GGCACC
BanII 219 GGGCTC G[GA]GC[CT]^C
1060 GAGCCC
BlpI 175 GCTCAGC GC^T[ACGT]AGC
1119 GCTGAGC
Bpu1102I 175 GCTCAGC GC^T[ACGT]AGC
1119 GCTGAGC
BsaAI 214 TACGTG [CT]AC^GT[GA]
505 TACGTG
BsgI 365 CTGCAC GTGCAG(16/14)
582 CTGCAC
BsmAI 1390 GAGAC GTCTC(1/5)
1052 GTCTC
BspMI 161 ACCTGC ACCTGC(4/8)
777 ACCTGC
CelII 175 GCTCAGC GC^T[ACGT]AGC
1119 GCTGAGC
FauI 186 GCGGG CCCGC(4/6)
711 GCGGG
HaeII 189 GGCGCT [GA]GCGC^[CT]
1156 AGCGCC
MaeII 215 ACGT A^CGT
506 ACGT
PstI 163 CTGCAG CTGCA^G
800 CTGCAG
PvuII 370 CAGCTG CAG^CTG
794 CAGCTG
TaqI 293 TCGA T^CGA
1244 TCGA
TthHB8I 293 TCGA T^CGA
1244 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 78 enzymes) #
#########################################
AccII AciI AfaI AluI AlwNI
AspHI AvaI AvaII BalI BbvI
BcnI BmyI BpmI BsaJI BseRI
BsiHKAI BsiYI BslI BsmFI BsoBI
Bsp1286I BsrI BssKI BstAPI BstF5I
BstNI BstUI Cac8I CfoI Cfr13I
DdeI DpnII DraII EaeI EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII HphI ItaI MaeIII MboI
MboII MluNI MnlI MscI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PleI
PpuMI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI StuI StyI TseI
Tsp45I TspRI XcmI