(Circular) (Six-base) MAPSORT of: pFN21AB0473.seq Check: 733 from: 1 to: 6202 pFN21AB0473 6202 bp With 182 enzymes: SgfI * April 15, 2010 09:33 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2791 2791 Size: 6202 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4012 278 Size: 53 83 186 936 2476 2468 Fragments arranged by size: 2476 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2217 3206 3545 1937 Size: 280 989 339 4594 Fragments arranged by size: 4594 989 339 280 Acc65I G'GTAC_C Cuts at: 2861 3523 2861 Size: 662 5540 AclI AA'CG_TT Cuts at: 1563 4329 4702 1563 Size: 2766 373 3063 Fragments arranged by size: 3063 2766 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2060 3477 4265 5649 5917 1632 Size: 428 1417 788 1384 268 1917 Fragments arranged by size: 1917 1417 1384 788 428 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6005 AflIII A'CryG_T Cuts at: 1203 2301 5101 6078 1203 Size: 1098 2800 977 1327 Fragments arranged by size: 2800 1327 1098 977 AgeI A'CCGG_T Cuts at: 5033 5033 Size: 6202 AhdI GACnn_n'nnGTC Cuts at: 4931 4931 Size: 6202 AleI CACnn'nnGTG Cuts at: 1166 2969 1166 Size: 1803 4399 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6202 AlwNI CAG_nnn'CTG Cuts at: 2804 2813 2914 3187 3945 5517 2804 Size: 9 101 273 758 1572 3489 Fragments arranged by size: 3489 1572 758 273 101 9 ApaLI G'TGCA_C Cuts at: 2522 4259 5415 2522 Size: 1737 1156 3309 Fragments arranged by size: 3309 1737 1156 ApoI r'AATT_y Cuts at: 1493 3511 3737 3889 5998 1493 Size: 2018 226 152 2109 1697 Fragments arranged by size: 2109 2018 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4756 160 Size: 872 3724 1606 Fragments arranged by size: 3724 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6202 AvaI C'yCGr_G Cuts at: 1870 1943 2057 2598 3101 3151 3527 1870 Size: 73 114 541 503 50 376 4545 Fragments arranged by size: 4545 541 503 376 114 73 50 BamHI G'GATC_C Cuts at: 3532 3899 3532 Size: 367 5835 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2861 2950 3523 4978 618 Size: 325 217 627 1074 89 573 1455 1842 Fragments arranged by size: 1842 1455 1074 627 573 325 217 89 BanII G_rGCy'C Cuts at: 1391 2228 3069 3521 1391 Size: 837 841 452 4072 Fragments arranged by size: 4072 841 837 452 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2670 928 Size: 971 771 4460 Fragments arranged by size: 4460 971 771 BbvCI CC'TCA_GC Cuts at: 2481 2481 Size: 6202 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4393 4427 1903 Size: 34 2456 34 3678 Fragments arranged by size: 3678 2456 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4094 5304 4094 Size: 1210 4992 BclI T'GATC_A Cuts at: 1548 1548 Size: 6202 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3413 4813 136 Size: 107 122 71 631 605 1741 1400 1525 Fragments arranged by size: 1741 1525 1400 631 605 122 107 71 BglII A'GATC_T Cuts at: 3366 6197 3366 Size: 2831 3371 BlpI GC'TnA_GC Cuts at: 2181 3125 3619 2181 Size: 944 494 4764 Fragments arranged by size: 4764 944 494 Bme1580I G_kGCm'C Cuts at: 1790 2526 2873 2921 4263 5419 1790 Size: 736 347 48 1342 1156 2573 Fragments arranged by size: 2573 1342 1156 736 347 48 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6202 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2650 3933 4891 Size: 416 53 83 399 24 1233 1283 958 Cuts at: 4891 442 Size: 1753 Fragments arranged by size: 1753 1283 1233 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6202 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2790 2976 3083 4862 1239 Size: 1551 186 107 1779 2579 Fragments arranged by size: 2579 1779 1551 186 107 Bpu10I CC'TnA_GC Cuts at: 2481 2829 2481 Size: 348 5854 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4327 5192 5490 4327 Size: 865 298 5039 Fragments arranged by size: 5039 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3062 4865 882 Size: 617 1563 1803 2219 Fragments arranged by size: 2219 1803 1563 617 BsaAI yAC'GTr Cuts at: 493 1204 2221 2512 3993 493 Size: 711 1017 291 1481 2702 Fragments arranged by size: 2702 1481 1017 711 291 BsaBI GATnn'nnATC Cuts at: 6196 6196 Size: 6202 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2054 4009 4391 5026 Size: 53 83 186 936 521 1955 382 635 Cuts at: 5026 275 Size: 1451 Fragments arranged by size: 1955 1451 936 635 521 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4635 5033 5307 5454 5865 4635 Size: 398 274 147 411 4972 Fragments arranged by size: 4972 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2838 2868 1178 Size: 30 165 30 1435 30 4512 Fragments arranged by size: 4512 1435 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2498 3104 3293 3353 3356 1186 Size: 1312 606 189 60 3 4032 Fragments arranged by size: 4032 1312 606 189 60 3 BseYI C'CCAG_C Cuts at: 2035 2242 2407 2438 2457 2857 2890 2907 2977 Size: 207 165 31 19 400 33 17 70 Cuts at: 2977 3052 5405 5901 2035 Size: 75 2353 496 2336 Fragments arranged by size: 2353 2336 496 400 207 165 75 70 33 31 19 17 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2355 2572 1163 Size: 573 619 217 4793 Fragments arranged by size: 4793 619 573 217 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3652 4413 5441 1540 Size: 275 186 1651 761 1028 2301 Fragments arranged by size: 2301 1651 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2024 2526 3141 4263 4348 5419 2024 Size: 502 615 1122 85 1071 2807 Fragments arranged by size: 2807 1122 1071 615 502 85 BsmI GAATG_Cn' Cuts at: 3713 3806 3713 Size: 93 6109 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2024 2228 2526 2873 2921 3069 3141 Size: 399 234 204 298 347 48 148 72 Cuts at: 3141 3521 4263 4348 5419 1391 Size: 380 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 380 347 298 234 204 148 85 72 48 BspEI T'CCGG_A Cuts at: 5865 5865 Size: 6202 BspHI T'CATG_A Cuts at: 4091 5821 4091 Size: 1730 4472 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2175 2791 3557 844 Size: 1331 616 766 3489 Fragments arranged by size: 3489 1331 766 616 BsrBI CCG'CTC Cuts at: 1394 1823 3112 4089 1394 Size: 429 1289 977 3507 Fragments arranged by size: 3507 1289 977 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4697 4871 65 Size: 1183 3449 174 1396 Fragments arranged by size: 3449 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4846 5033 1700 Size: 110 3036 187 2869 Fragments arranged by size: 3036 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6202 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6202 BssSI C'ACGA_G Cuts at: 4262 5274 4262 Size: 1012 5190 BstAPI GCAn_nnn'nTGC Cuts at: 794 2804 2813 3187 794 Size: 2010 9 374 3809 Fragments arranged by size: 3809 2010 374 9 BstBI TT'CG_AA Cuts at: 5006 5006 Size: 6202 BstEII G'GTnAC_C Cuts at: 1184 2998 1184 Size: 1814 4388 BstXI CCAn_nnnn'nTGG Cuts at: 2954 2954 Size: 6202 BstYI r'GATC_y Cuts at: 1972 3366 3532 3899 4284 4301 5753 5839 5851 Size: 1394 166 367 385 17 1452 86 12 Cuts at: 5851 6197 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1394 385 367 346 166 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2097 513 Size: 552 939 93 4618 Fragments arranged by size: 4618 939 552 93 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2122 487 Size: 683 56 896 4567 Fragments arranged by size: 4567 896 683 56 BtsI GCAGTG_nn' Cuts at: 2811 2844 3718 4512 4532 2811 Size: 33 874 794 20 4481 Fragments arranged by size: 4481 874 794 33 20 ClaI AT'CG_AT Cuts at: 1554 3249 1554 Size: 1695 4507 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6167 DraI TTT'AAA Cuts at: 3506 3506 Size: 6202 DraIII CAC_nnn'GTG Cuts at: 3175 3175 Size: 6202 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5209 809 Size: 507 3893 1802 Fragments arranged by size: 3893 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2199 2704 2841 Size: 54 1425 50 275 18 369 505 137 Cuts at: 2841 3036 3113 3424 3439 3649 4538 6167 8 Size: 195 77 311 15 210 889 1629 43 Fragments arranged by size: 1629 1425 889 505 369 311 275 210 195 137 77 54 50 43 18 15 EagI C'GGCC_G Cuts at: 1537 1812 3649 1537 Size: 275 1837 4090 Fragments arranged by size: 4090 1837 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3081 4132 1353 Size: 1728 1051 3423 Fragments arranged by size: 3423 1728 1051 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2249 4789 5163 5309 387 Size: 1064 138 660 2540 374 146 1280 Fragments arranged by size: 2540 1280 1064 660 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2060 2790 2976 3083 3477 4265 4862 Size: 393 428 730 186 107 394 788 597 Cuts at: 4862 5649 5917 1239 Size: 787 268 1524 Fragments arranged by size: 1524 788 787 730 597 428 394 393 268 186 107 EcoNI CCTnn'n_nnAGG Cuts at: 2078 2078 Size: 6202 EcoO109I rG'GnC_Cy Cuts at: 2071 2359 2530 2602 3415 2071 Size: 288 171 72 813 4858 Fragments arranged by size: 4858 813 288 171 72 EcoRI G'AATT_C Cuts at: 3511 3889 3511 Size: 378 5824 FspI TGC'GCA Cuts at: 3224 4708 3224 Size: 1484 4718 HaeII r_GCGC'y Cuts at: 1678 2198 3165 5349 1678 Size: 520 967 2184 2531 Fragments arranged by size: 2531 2184 967 520 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2164 2196 2660 2692 2812 Size: 32 135 32 592 32 464 32 120 Cuts at: 2812 2844 4843 4875 4917 4949 1373 Size: 32 1999 32 42 32 2626 Fragments arranged by size: 2626 1999 592 464 135 120 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3546 3792 669 Size: 988 281 1608 246 3079 Fragments arranged by size: 3079 1608 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6202 HpaI GTT'AAC Cuts at: 3792 3792 Size: 6202 KpnI G_GTAC'C Cuts at: 2865 3527 2865 Size: 662 5540 MfeI C'AATT_G Cuts at: 3801 3801 Size: 6202 MluI A'CGCG_T Cuts at: 6078 6078 Size: 6202 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3071 5316 5500 1305 Size: 1766 2245 184 2007 Fragments arranged by size: 2245 2007 1766 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2201 2706 3038 10 Size: 54 1425 343 369 505 332 3174 Fragments arranged by size: 3174 1425 505 369 343 332 54 MslI CAynn'nnrTG Cuts at: 518 1166 2300 2426 2510 2969 3176 4160 4519 Size: 648 1134 126 84 459 207 984 359 Cuts at: 4519 4678 518 Size: 159 2042 Fragments arranged by size: 2042 1134 984 648 459 359 207 159 126 84 MspA1I CmG'CkG Cuts at: 1606 2185 2191 2377 2789 2801 2852 3429 3618 Size: 579 6 186 412 12 51 577 189 Cuts at: 3618 4295 5443 5688 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 579 577 412 245 189 186 51 12 6 NcoI C'CATG_G Cuts at: 513 1065 2004 2097 513 Size: 552 939 93 4618 Fragments arranged by size: 4618 939 552 93 NdeI CA'TA_TG Cuts at: 387 387 Size: 6202 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6202 NotI GC'GGCC_GC Cuts at: 3649 3649 Size: 6202 NspI r_CATG'y Cuts at: 1132 1229 2305 3560 5105 1132 Size: 97 1076 1255 1545 2229 Fragments arranged by size: 2229 1545 1255 1076 97 PasI CC'CwG_GG Cuts at: 1348 2866 2914 1348 Size: 1518 48 4636 Fragments arranged by size: 4636 1518 48 PciI A'CATG_T Cuts at: 2301 5101 2301 Size: 2800 3402 PflMI CCAn_nnn'nTGG Cuts at: 1108 2914 1108 Size: 1806 4396 PfoI T'CCnGG_A Cuts at: 3060 3060 Size: 6202 PmeI GTTT'AAAC Cuts at: 3506 3506 Size: 6202 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4271 4303 5784 5816 4271 Size: 32 1481 32 4657 Fragments arranged by size: 4657 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2359 2530 2602 2359 Size: 171 72 5959 Fragments arranged by size: 5959 171 72 PshAI GACnn'nnGTC Cuts at: 1613 2884 1613 Size: 1271 4931 PsiI TTA'TAA Cuts at: 3772 3772 Size: 6202 PstI C_TGCA'G Cuts at: 830 2172 2809 3554 830 Size: 1342 637 745 3478 Fragments arranged by size: 3478 1342 745 637 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6202 PvuII CAG'CTG Cuts at: 2377 2801 2377 Size: 424 5778 SalI G'TCGA_C Cuts at: 1936 3544 1936 Size: 1608 4594 SanDI GG'GwC_CC Cuts at: 2602 2602 Size: 6202 SbfI CC_TGCA'GG Cuts at: 3554 3554 Size: 6202 ScaI AGT'ACT Cuts at: 2711 4450 6162 2711 Size: 1739 1712 2751 Fragments arranged by size: 2751 1739 1712 SfcI C'TryA_G Cuts at: 826 1045 2168 2805 3207 3550 4685 5366 5557 Size: 219 1123 637 402 343 1135 681 191 Cuts at: 5557 826 Size: 1471 Fragments arranged by size: 1471 1135 1123 681 637 402 343 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 3413 3413 Size: 6202 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6202 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6202 SmaI CCC'GGG Cuts at: 1872 2600 3529 1872 Size: 728 929 4545 Fragments arranged by size: 4545 929 728 SmlI C'TyrA_G Cuts at: 820 1017 1943 4306 5207 5469 820 Size: 197 926 2363 901 262 1553 Fragments arranged by size: 2363 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3993 493 Size: 3500 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6202 SphI G_CATG'C Cuts at: 1132 3560 1132 Size: 2428 3774 SspI AAT'ATT Cuts at: 5 52 4126 5 Size: 47 4074 2081 Fragments arranged by size: 4074 2081 47 StuI AGG'CCT Cuts at: 1517 2267 2567 2775 1517 Size: 750 300 208 4944 Fragments arranged by size: 4944 750 300 208 StyI C'CwwG_G Cuts at: 513 1065 2004 2097 2382 3240 3641 513 Size: 552 939 93 285 858 401 3074 Fragments arranged by size: 3074 939 858 552 401 285 93 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2690 4229 4246 4399 4584 5029 325 Size: 1255 1110 1539 17 153 185 445 1498 Fragments arranged by size: 1539 1498 1255 1110 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2709 4448 6160 Size: 274 80 33 51 1443 732 1739 1712 Cuts at: 6160 96 Size: 138 Fragments arranged by size: 1739 1712 1443 732 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2838 2870 6162 6194 2838 Size: 32 3292 32 2846 Fragments arranged by size: 3292 2846 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2315 1316 Size: 999 5203 XbaI T'CTAG_A Cuts at: 3538 3538 Size: 6202 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2170 3010 3419 2170 Size: 840 409 4953 Fragments arranged by size: 4953 840 409 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6202 XmaI C'CCGG_G Cuts at: 1870 2598 3527 1870 Size: 728 929 4545 Fragments arranged by size: 4545 929 728 XmnI GAAnn'nnTTC Cuts at: 4331 4331 Size: 6202 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4010 276 Size: 53 83 186 936 2476 2468 Fragments arranged by size: 2476 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SapI SexAI SfoI SrfI SwaI