Restriction map for pFN21AB0473
 (Linear) (Six-base) MAPSORT of: KIBB0473.seq  Check: 7023  from: 1  to: 1497



KIBB0473.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:14  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     786    1497 
   Size:        786     711
 
AccI GT'mk_AC

Cuts at:      0     212    1201    1497 
   Size:        212     989     296

  Fragments arranged by size: 

                 989     296     212
 
Acc65I G'GTAC_C

Cuts at:      0     856    1497 
   Size:        856     641
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      55    1472    1497 
   Size:         55    1417      25

  Fragments arranged by size: 

                1417      55      25
 
AflIII A'CryG_T

Cuts at:      0     296    1497 
   Size:        296    1201
 
AleI CACnn'nnGTG

Cuts at:      0     964    1497 
   Size:        964     533
 
AlwNI CAG_nnn'CTG

Cuts at:      0     799     808     909    1182    1497 
   Size:        799       9     101     273     315

  Fragments arranged by size: 

                 799     315     273     101       9
 
ApaLI G'TGCA_C

Cuts at:      0     517    1497 
   Size:        517     980
 
AvaI C'yCGr_G

Cuts at:      0      52     593    1096    1146    1497 
   Size:         52     541     503      50     351

  Fragments arranged by size: 

                 541     503     351      52      50
 
BanI G'GyrC_C

Cuts at:      0     856     945    1497 
   Size:        856      89     552

  Fragments arranged by size: 

                 856     552      89
 
BanII G_rGCy'C

Cuts at:      0     223    1064    1497 
   Size:        223     841     433

  Fragments arranged by size: 

                 841     433     223
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     665    1497 
   Size:        665     832
 
BbvCI CC'TCA_GC

Cuts at:      0     476    1497 
   Size:        476    1021
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1408    1497 
   Size:       1408      89
 
BglII A'GATC_T

Cuts at:      0    1361    1497 
   Size:       1361     136
 
BlpI GC'TnA_GC

Cuts at:      0     176    1120    1497 
   Size:        176     944     377

  Fragments arranged by size: 

                 944     377     176
 
Bme1580I G_kGCm'C

Cuts at:      0     521     868     916    1497 
   Size:        521     347      48     581

  Fragments arranged by size: 

                 581     521     347      48
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     645    1497 
   Size:        645     852
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     785     971    1078    1497 
   Size:        785     186     107     419

  Fragments arranged by size: 

                 785     419     186     107
 
Bpu10I CC'TnA_GC

Cuts at:      0     476     824    1497 
   Size:        476     348     673

  Fragments arranged by size: 

                 673     476     348
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1057    1497 
   Size:       1057     440
 
BsaAI yAC'GTr

Cuts at:      0     216     507    1497 
   Size:        216     291     990

  Fragments arranged by size: 

                 990     291     216
 
BsaHI Gr'CG_yC

Cuts at:      0      49    1497 
   Size:         49    1448
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     833     863    1497 
   Size:        833      30     634

  Fragments arranged by size: 

                 833     634      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     493    1099    1288    1348    1351    1497 
   Size:        493     606     189      60       3     146

  Fragments arranged by size: 

                 606     493     189     146      60       3
 
BseYI C'CCAG_C

Cuts at:      0      30     237     402     433     452     852     885     902 
   Size:         30     207     165      31      19     400      33      17

Cuts at:    902     972    1047    1497 
   Size:         70      75     450

  Fragments arranged by size: 

                 450     400     207     165      75      70      33      31
                  30      19      17
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     350     567    1497 
   Size:        350     217     930

  Fragments arranged by size: 

                 930     350     217
 
BsiHKAI G_wGCw'C

Cuts at:      0      19     521    1136    1497 
   Size:         19     502     615     361

  Fragments arranged by size: 

                 615     502     361      19
 
Bsp1286I G_dGCh'C

Cuts at:      0      19     223     521     868     916    1064    1136    1497 
   Size:         19     204     298     347      48     148      72     361

  Fragments arranged by size: 

                 361     347     298     204     148      72      48      19
            
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     170     786    1497 
   Size:        170     616     711

  Fragments arranged by size: 

                 711     616     170
 
BsrBI CCG'CTC

Cuts at:      0    1107    1497 
   Size:       1107     390
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     799     808    1182    1497 
   Size:        799       9     374     315

  Fragments arranged by size: 

                 799     374     315       9
 
BstEII G'GTnAC_C

Cuts at:      0     993    1497 
   Size:        993     504
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     949    1497 
   Size:        949     548
 
BstYI r'GATC_y

Cuts at:      0    1361    1497 
   Size:       1361     136
 
BtgI C'CryG_G

Cuts at:      0      92    1497 
   Size:         92    1405
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     117    1497 
   Size:        117    1380
 
BtsI GCAGTG_nn'

Cuts at:      0     806     839    1497 
   Size:        806      33     658

  Fragments arranged by size: 

                 806     658      33
 
ClaI AT'CG_AT

Cuts at:      0    1244    1497 
   Size:       1244     253
 
DraIII CAC_nnn'GTG

Cuts at:      0    1170    1497 
   Size:       1170     327
 
EaeI y'GGCC_r

Cuts at:      0     194     699     836    1031    1108    1419    1434    1497 
   Size:        194     505     137     195      77     311      15      63

  Fragments arranged by size: 

                 505     311     195     194     137      77      63      15
            
 
EarI CTCTTCn'nnn_

Cuts at:      0    1076    1497 
   Size:       1076     421
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     244    1497 
   Size:        244    1253
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      55     785     971    1078    1472    1497 
   Size:         55     730     186     107     394      25

  Fragments arranged by size: 

                 730     394     186     107      55      25
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      73    1497 
   Size:         73    1424
 
EcoO109I rG'GnC_Cy

Cuts at:      0      66     354     525     597    1410    1497 
   Size:         66     288     171      72     813      87

  Fragments arranged by size: 

                 813     288     171      87      72      66
 
FspI TGC'GCA

Cuts at:      0    1219    1497 
   Size:       1219     278
 
HaeII r_GCGC'y

Cuts at:      0     193    1160    1497 
   Size:        193     967     337

  Fragments arranged by size: 

                 967     337     193
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     159     191     655     687     807     839    1497 
   Size:        159      32     464      32     120      32     658

  Fragments arranged by size: 

                 658     464     159     120      32      32      32
 
KpnI G_GTAC'C

Cuts at:      0     860    1497 
   Size:        860     637
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1066    1497 
   Size:       1066     431
 
MscI TGG'CCA

Cuts at:      0     196     701    1033    1497 
   Size:        196     505     332     464

  Fragments arranged by size: 

                 505     464     332     196
 
MslI CAynn'nnrTG

Cuts at:      0     295     421     505     964    1171    1497 
   Size:        295     126      84     459     207     326

  Fragments arranged by size: 

                 459     326     295     207     126      84
 
MspA1I CmG'CkG

Cuts at:      0     180     186     372     784     796     847    1424    1497 
   Size:        180       6     186     412      12      51     577      73

  Fragments arranged by size: 

                 577     412     186     180      73      51      12       6
            
 
NcoI C'CATG_G

Cuts at:      0      92    1497 
   Size:         92    1405
 
NspI r_CATG'y

Cuts at:      0     300    1497 
   Size:        300    1197
 
PasI CC'CwG_GG

Cuts at:      0     861     909    1497 
   Size:        861      48     588

  Fragments arranged by size: 

                 861     588      48
 
PciI A'CATG_T

Cuts at:      0     296    1497 
   Size:        296    1201
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     909    1497 
   Size:        909     588
 
PfoI T'CCnGG_A

Cuts at:      0    1055    1497 
   Size:       1055     442
 
PpuMI rG'GwC_Cy

Cuts at:      0     354     525     597    1497 
   Size:        354     171      72     900

  Fragments arranged by size: 

                 900     354     171      72
 
PshAI GACnn'nnGTC

Cuts at:      0     879    1497 
   Size:        879     618
 
PstI C_TGCA'G

Cuts at:      0     167     804    1497 
   Size:        167     637     693

  Fragments arranged by size: 

                 693     637     167
 
PvuII CAG'CTG

Cuts at:      0     372     796    1497 
   Size:        372     424     701

  Fragments arranged by size: 

                 701     424     372
 
SanDI GG'GwC_CC

Cuts at:      0     597    1497 
   Size:        597     900
 
ScaI AGT'ACT

Cuts at:      0     706    1497 
   Size:        706     791
 
SfcI C'TryA_G

Cuts at:      0     163     800    1202    1497 
   Size:        163     637     402     295

  Fragments arranged by size: 

                 637     402     295     163
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0    1408    1497 
   Size:       1408      89
 
SmaI CCC'GGG

Cuts at:      0     595    1497 
   Size:        595     902
 
StuI AGG'CCT

Cuts at:      0     262     562     770    1497 
   Size:        262     300     208     727

  Fragments arranged by size: 

                 727     300     262     208
 
StyI C'CwwG_G

Cuts at:      0      92     377    1235    1497 
   Size:         92     285     858     262

  Fragments arranged by size: 

                 858     285     262      92
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     685    1497 
   Size:        685     812
 
TatI w'GTAC_w

Cuts at:      0     704    1497 
   Size:        704     793
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     833     865    1497 
   Size:        833      32     632

  Fragments arranged by size: 

                 833     632      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     310    1497 
   Size:        310    1187
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     165    1005    1414    1497 
   Size:        165     840     409      83

  Fragments arranged by size: 

                 840     409     165      83
 
XmaI C'CCGG_G

Cuts at:      0     593    1497 
   Size:        593     904
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AcuI   AflIII     AleI    AlwNI    ApaLI
     AvaI     BanI    BanII     BbsI    BbvCI     BglI    BglII     BlpI
 Bme1580I     BmrI     BpmI   Bpu10I     BsaI    BsaAI    BsaHI    BsaXI
    BseRI    BseYI     BsgI  BsiHKAI Bsp1286I    BspMI    BsrBI   BstAPI
   BstEII    BstXI    BstYI     BtgI    BtgZI     BtsI     ClaI   DraIII
     EaeI     EarI     EciI  Eco57MI    EcoNI EcoO109I     FspI    HaeII
    Hin4I     KpnI     MmeI     MscI     MslI   MspA1I     NcoI     NspI
     PasI     PciI    PflMI     PfoI    PpuMI    PshAI     PstI    PvuII
    SanDI     ScaI     SfcI     SfiI     SmaI     StuI     StyI    TaqII
     TatI     TstI  Tth111I     XcmI     XmaI

 Enzymes that do not cut: 

    AatII     AclI     AfeI    AflII     AgeI     AhdI     AlfI     AloI
     ApaI     ApoI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI
     BbeI     BcgI    BciVI     BclI    BfrBI    BmgBI     BmtI     BplI
    BpuEI    BsaBI    BsaWI    BsiEI    BsiWI     BsmI    BsmBI    BspEI
    BspHI    BsrDI    BsrFI    BsrGI   BssHII    BssSI    BstBI  BstZ17I
   Bsu36I    CspCI     DraI     DrdI     EagI  EcoICRI    EcoRI    EcoRV
     FalI     FseI    FspAI   HincII  HindIII     HpaI     KasI     MfeI
     MluI     NaeI     NarI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     PacI     PmeI     PmlI     PpiI     PsiI   PspOMI    PspXI
     PsrI     PvuI    RsrII     SacI    SacII     SalI     SapI     SbfI
    SexAI     SfoI     SgfI    SgrAI     SmlI    SnaBI     SpeI     SphI
     SrfI     SspI     SwaI     XbaI     XhoI     XmnI     ZraI
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