Restriction map for
pFN21AB0463
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# NO CUT ENZYMES ( 119 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AflIII AgeI AhdI
Alw44I AlwI Aor51HI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII AvrII BamHI
BbrPI BciVI BfaI BlnI BsaBI
BseAI BseRI BsgI BsiWI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstZ17I ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FseI
FspI HindIII HpaI KpnI KspI
MaeI MamI MfeI MluI MroI
MunI MvnI NaeI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PflFI PinAI PmaCI PmeI
PmlI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI SbfI ScaI
SexAI SfiI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI Tsp509I Tth111I
XbaI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 35 enzymes) #
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AflII 1395 CTTAAG C^TTAAG
AocI 196 CCTGAGG CC^T[ACGT]AGG
AspHI 1225 GAGCTC G[AT]GC[AT]^C
BbvCI 46 CCTCAGC CCTCAGC(-5/-2)
BclI 863 TGATCA T^GATCA
BcnI 1248 CCCGG CC^[GC]GG
BfrI 1395 CTTAAG C^TTAAG
BglII 1433 AGATCT A^GATCT
BsaAI 475 TACGTG [CT]AC^GT[GA]
BsaI 1014 GGTCTC GGTCTC(1/5)
BsaWI 1451 ACCGGA [AT]^CCGG[AT]
BsiEI 872 CGGTCG CG[GA][CT]^CG
BsiHKAI 1225 GAGCTC G[AT]GC[AT]^C
BsmI 548 GCATTC GAATGC(1/-1)
BsrDI 867 CATTGC GCAATG(2/0)
BsrFI 851 ACCGGC [GA]^CCGG[CT]
BstYI 1433 AGATCT [GA]^GATC[CT]
Bsu36I 196 CCTGAGG CC^T[ACGT]AGG
Cfr10I 851 ACCGGC [GA]^CCGG[CT]
EarI 1228 CTCTTC CTCTTC(1/4)
Eco81I 196 CCTGAGG CC^T[ACGT]AGG
FbaI 863 TGATCA T^GATCA
Ksp632I 1228 CTCTTC CTCTTC(1/4)
MaeIII 58 GTGAC ^GT[ACGT]AC
MflI 1433 AGATCT [GA]^GATC[CT]
MseI 1396 TTAA T^TAA
NciI 1248 CCCGG CC^[GC]GG
NcoI 1256 CCATGG C^CATGG
NspI 1076 GCATGT [GA]CATG^[CT]
PshAI 66 GACCCTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 1225 GAGCTC GAGCT^C
SapI 1227 GCTCTTC GCTCTTC(1/4)
Tru9I 1396 TTAA T^TAA
Tsp45I 58 GTGAC ^GT[GC]AC
XhoII 1433 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 20 enzymes) #
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ApaI 242 GGGCCC GGGCC^C
1336 GGGCCC
BbsI 1003 GTCTTC GAAGAC(2/6)
1345 GTCTTC
BcgI 708 CGAGGGGCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
548 GCATTCTGATCG
BlpI 772 GCTGAGC GC^T[ACGT]AGC
885 GCTGAGC
Bpu1102I 772 GCTGAGC GC^T[ACGT]AGC
885 GCTGAGC
BpuAI 1003 GTCTTC GAAGAC(2/6)
1345 GTCTTC
BsmBI 1146 CGTCTC CGTCTC(1/5)
172 GAGACG
BspMI 500 ACCTGC ACCTGC(4/8)
633 ACCTGC
BsrBI 808 GAGCGG CCGCTC(-3/-3)
1033 GAGCGG
BstXI 470 CCATGTACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1256 CCATGGCTGTGG
CelII 772 GCTGAGC GC^T[ACGT]AGC
885 GCTGAGC
HgaI 519 GACGC GACGC(5/10)
1050 GACGC
HincII 340 GTCAAC GT[CT]^[GA]AC
994 GTCAAC
HindII 340 GTCAAC GT[CT]^[GA]AC
994 GTCAAC
PaeR7I 706 CTCGAG C^TCGAG
1412 CTCGAG
PleI 577 GAGTC GAGTC(4/5)
726 GAGTC
SfaNI 905 GCATC GCATC(5/9)
935 GCATC
TfiI 425 GATTC G^A[AT]TC
702 GAATC
XcmI 257 CCATCTTTTCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
891 CCAGCAGCTCTCTGG
XhoI 706 CTCGAG C^TCGAG
1412 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 84 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwNI
AvaI AvaII BalI BanI BanII
BbeI BbvI BglI BmyI BpmI
BsaHI BsaJI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BsrI BssKI
BstF5I BstNI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI KasI MaeII MboI MboII
MluNI MnlI MscI MslI MspA1I
MspI MvaI MwoI NarI NdeII
NlaIII NlaIV PflMI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfcI SfoI SmlI StyI TaqI
TseI TspRI TthHB8I Van91I