(Circular) (Six-base) MAPSORT of: pFN21AB0463.seq Check: 6756 from: 1 to: 6232 pFN21AB0463 6232 bp With 182 enzymes: SgfI * April 15, 2010 09:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2514 2514 Size: 6232 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4042 278 Size: 53 83 186 936 2506 2468 Fragments arranged by size: 2506 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3575 1937 Size: 1638 4594 Acc65I G'GTAC_C Cuts at: 3553 3553 Size: 6232 AclI AA'CG_TT Cuts at: 1563 4359 4732 1563 Size: 2796 373 3063 Fragments arranged by size: 3063 2796 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2756 2804 3220 4295 5679 5947 1632 Size: 1124 48 416 1075 1384 268 1917 Fragments arranged by size: 1917 1384 1124 1075 416 268 48 AflII C'TTAA_G Cuts at: 820 1017 3400 820 Size: 197 2383 3652 Fragments arranged by size: 3652 2383 197 AflIII A'CryG_T Cuts at: 1203 5131 6108 1203 Size: 3928 977 1327 Fragments arranged by size: 3928 1327 977 AgeI A'CCGG_T Cuts at: 5063 5063 Size: 6232 AhdI GACnn_n'nnGTC Cuts at: 4961 4961 Size: 6232 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6232 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6232 AlwNI CAG_nnn'CTG Cuts at: 2572 2839 2866 3975 5547 2572 Size: 267 27 1109 1572 3257 Fragments arranged by size: 3257 1572 1109 267 27 ApaI G_GGCC'C Cuts at: 2251 3345 2251 Size: 1094 5138 ApaLI G'TGCA_C Cuts at: 4289 5445 4289 Size: 1156 5076 ApoI r'AATT_y Cuts at: 1493 3541 3767 3919 6028 1493 Size: 2048 226 152 2109 1697 Fragments arranged by size: 2109 2048 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4786 160 Size: 872 3754 1606 Fragments arranged by size: 3754 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6232 AvaI C'yCGr_G Cuts at: 1870 1943 2675 2711 3417 3557 1870 Size: 73 732 36 706 140 4545 Fragments arranged by size: 4545 732 706 140 73 36 BamHI G'GATC_C Cuts at: 3562 3929 3562 Size: 367 5865 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2228 2441 2470 2513 3048 Size: 325 217 627 441 213 29 43 535 Cuts at: 3048 3553 5008 618 Size: 505 1455 1842 Fragments arranged by size: 1842 1455 627 535 505 441 325 217 213 43 29 BanII G_rGCy'C Cuts at: 1391 2251 2684 3234 3345 3551 1391 Size: 860 433 550 111 206 4072 Fragments arranged by size: 4072 860 550 433 206 111 BbeI G_GCGC'C Cuts at: 2445 2517 3052 2445 Size: 72 535 5625 Fragments arranged by size: 5625 535 72 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3001 3343 928 Size: 971 1102 342 3817 Fragments arranged by size: 3817 1102 971 342 BbvCI CC'TCA_GC Cuts at: 2052 2052 Size: 6232 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2542 2576 2702 2736 4423 Size: 34 50 34 521 34 126 34 1687 Cuts at: 4423 4457 1903 Size: 34 3678 Fragments arranged by size: 3678 1687 521 126 50 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4124 5334 4124 Size: 1210 5022 BclI T'GATC_A Cuts at: 1548 2868 1548 Size: 1320 4912 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2382 2748 3078 Size: 107 122 71 631 605 710 366 330 Cuts at: 3078 4843 136 Size: 1765 1525 Fragments arranged by size: 1765 1525 710 631 605 366 330 122 107 71 BglII A'GATC_T Cuts at: 3438 6227 3438 Size: 2789 3443 BlpI GC'TnA_GC Cuts at: 2778 2891 3649 2778 Size: 113 758 5361 Fragments arranged by size: 5361 758 113 Bme1580I G_kGCm'C Cuts at: 1790 2251 2475 2597 3345 4293 5449 1790 Size: 461 224 122 748 948 1156 2573 Fragments arranged by size: 2573 1156 948 748 461 224 122 BmgBI CAC'GTC Cuts at: 1318 2181 1318 Size: 863 5369 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2076 3963 4921 Size: 416 53 83 399 24 659 1887 958 Cuts at: 4921 442 Size: 1753 Fragments arranged by size: 1887 1753 958 659 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6232 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2208 2787 2816 2825 3146 4892 1239 Size: 969 579 29 9 321 1746 2579 Fragments arranged by size: 2579 1746 969 579 321 29 9 Bpu10I CC'TnA_GC Cuts at: 2052 2052 Size: 6232 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3524 4357 5222 5520 3524 Size: 833 865 298 4236 Fragments arranged by size: 4236 865 833 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3025 4895 882 Size: 617 1526 1870 2219 Fragments arranged by size: 2219 1870 1526 617 BsaAI yAC'GTr Cuts at: 493 1204 2482 4023 493 Size: 711 1278 1541 2702 Fragments arranged by size: 2702 1541 1278 711 BsaBI GATnn'nnATC Cuts at: 6226 6226 Size: 6232 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2442 2514 3049 4039 Size: 53 83 186 936 909 72 535 990 Cuts at: 4039 4421 5056 275 Size: 382 635 1451 Fragments arranged by size: 1451 990 936 909 635 535 382 186 83 72 53 BsaWI w'CCGG_w Cuts at: 3456 4665 5063 5337 5484 5895 3456 Size: 1209 398 274 147 411 3793 Fragments arranged by size: 3793 1209 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2628 2658 2979 3009 1178 Size: 30 165 30 1225 30 321 30 4401 Fragments arranged by size: 4401 1225 321 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6232 BseYI C'CCAG_C Cuts at: 2185 2210 2365 2572 2751 3376 3491 5435 5931 Size: 25 155 207 179 625 115 1944 496 Cuts at: 5931 2185 Size: 2486 Fragments arranged by size: 2486 1944 625 496 207 179 155 115 25 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5659 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2880 3682 4443 5471 1540 Size: 275 186 879 802 761 1028 2301 Fragments arranged by size: 2301 1028 879 802 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3234 4293 4378 5449 3234 Size: 1059 85 1071 4017 Fragments arranged by size: 4017 1071 1059 85 BsmI GAATG_Cn' Cuts at: 2553 3743 3836 2553 Size: 1190 93 4949 Fragments arranged by size: 4949 1190 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2171 3157 2171 Size: 986 5246 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2251 2475 2597 2684 3234 3345 3551 Size: 399 461 224 122 87 550 111 206 Cuts at: 3551 4293 4378 5449 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 550 461 399 224 206 122 111 87 85 BspEI T'CCGG_A Cuts at: 5895 5895 Size: 6232 BspHI T'CATG_A Cuts at: 4121 5851 4121 Size: 1730 4502 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2514 2647 3587 844 Size: 1670 133 940 3489 Fragments arranged by size: 3489 1670 940 133 BsrBI CCG'CTC Cuts at: 1394 1823 2815 3040 4119 1394 Size: 429 992 225 1079 3507 Fragments arranged by size: 3507 1079 992 429 225 BsrDI GCAATG_nn' Cuts at: 65 1248 2871 4727 4901 65 Size: 1183 1623 1856 174 1396 Fragments arranged by size: 1856 1623 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2856 4876 5063 1700 Size: 110 1046 2020 187 2869 Fragments arranged by size: 2869 2020 1046 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6232 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6232 BssSI C'ACGA_G Cuts at: 4292 5304 4292 Size: 1012 5220 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6232 BstBI TT'CG_AA Cuts at: 5036 5036 Size: 6232 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6232 BstXI CCAn_nnnn'nTGG Cuts at: 2482 3268 2482 Size: 786 5446 BstYI r'GATC_y Cuts at: 1972 3438 3562 3929 4314 4331 5783 5869 5881 Size: 1466 124 367 385 17 1452 86 12 Cuts at: 5881 6227 1972 Size: 346 1977 Fragments arranged by size: 1977 1466 1452 385 367 346 124 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2202 2202 Size: 6232 BtgI C'CryG_G Cuts at: 513 1065 2256 2271 3261 513 Size: 552 1191 15 990 3484 Fragments arranged by size: 3484 1191 990 552 15 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5493 Fragments arranged by size: 5493 683 56 BtsI GCAGTG_nn' Cuts at: 2595 2612 3748 4542 4562 2595 Size: 17 1136 794 20 4265 Fragments arranged by size: 4265 1136 794 20 17 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6232 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6197 DraI TTT'AAA Cuts at: 3536 3536 Size: 6232 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5239 809 Size: 507 3923 1802 Fragments arranged by size: 3923 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2259 2388 2538 Size: 54 1425 50 275 18 429 129 150 Cuts at: 2538 2609 2816 3162 3225 3679 4568 6197 8 Size: 71 207 346 63 454 889 1629 43 Fragments arranged by size: 1629 1425 889 454 429 346 275 207 150 129 71 63 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3679 1537 Size: 275 1867 4090 Fragments arranged by size: 4090 1867 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3239 4162 1353 Size: 1886 923 3423 Fragments arranged by size: 3423 1886 923 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4819 5193 5339 387 Size: 1064 138 3230 374 146 1280 Fragments arranged by size: 3230 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3232 3232 Size: 6232 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2208 2756 2787 2804 2816 2825 3146 Size: 393 576 548 31 17 12 9 321 Cuts at: 3146 3220 4295 4892 5679 5947 1239 Size: 74 1075 597 787 268 1524 Fragments arranged by size: 1524 1075 787 597 576 548 393 321 268 74 31 17 12 9 EcoO109I rG'GnC_Cy Cuts at: 2070 2198 2207 2248 2717 3245 3341 3342 2070 Size: 128 9 41 469 528 96 1 4960 Fragments arranged by size: 4960 528 469 128 96 41 9 1 EcoRI G'AATT_C Cuts at: 3541 3919 3541 Size: 378 5854 FspI TGC'GCA Cuts at: 4738 4738 Size: 6232 HaeII r_GCGC'y Cuts at: 1678 2445 2517 2743 3052 5379 1678 Size: 767 72 226 309 2327 2531 Fragments arranged by size: 2531 2327 767 309 226 72 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2418 2450 3046 3078 3097 Size: 32 135 32 846 32 596 32 19 Cuts at: 3097 3129 3425 3451 3457 3483 4873 4905 4947 Size: 32 296 26 6 26 1390 32 42 Cuts at: 4947 4979 1373 Size: 32 2626 Fragments arranged by size: 2626 1390 846 596 296 135 42 32 32 32 32 32 32 32 26 26 19 6 HincII GTy'rAC Cuts at: 669 1657 1938 2347 3001 3576 3822 669 Size: 988 281 409 654 575 246 3079 Fragments arranged by size: 3079 988 654 575 409 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6232 HpaI GTT'AAC Cuts at: 3822 3822 Size: 6232 KasI G'GCGC_C Cuts at: 2441 2513 3048 2441 Size: 72 535 5625 Fragments arranged by size: 5625 535 72 KpnI G_GTAC'C Cuts at: 3557 3557 Size: 6232 MfeI C'AATT_G Cuts at: 3831 3831 Size: 6232 MluI A'CGCG_T Cuts at: 6108 6108 Size: 6232 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2044 3306 5346 5530 1305 Size: 739 1262 2040 184 2007 Fragments arranged by size: 2040 2007 1262 739 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2261 2390 2540 2611 3164 Size: 54 1425 343 429 129 150 71 553 Cuts at: 3164 10 Size: 3078 Fragments arranged by size: 3078 1425 553 429 343 150 129 71 54 MslI CAynn'nnrTG Cuts at: 518 1166 2480 3101 3266 3308 4190 4549 4708 Size: 648 1314 621 165 42 882 359 159 Cuts at: 4708 518 Size: 2042 Fragments arranged by size: 2042 1314 882 648 621 359 165 159 42 MspA1I CmG'CkG Cuts at: 1606 2653 2890 3446 3648 4325 5473 5718 1606 Size: 1047 237 556 202 677 1148 245 2120 Fragments arranged by size: 2120 1148 1047 677 556 245 237 202 NarI GG'CG_CC Cuts at: 2442 2514 3049 2442 Size: 72 535 5625 Fragments arranged by size: 5625 535 72 NcoI C'CATG_G Cuts at: 513 1065 3261 513 Size: 552 2196 3484 Fragments arranged by size: 3484 2196 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6232 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6232 NotI GC'GGCC_GC Cuts at: 3679 3679 Size: 6232 NspI r_CATG'y Cuts at: 1132 1229 3085 3590 5135 1132 Size: 97 1856 505 1545 2229 Fragments arranged by size: 2229 1856 1545 505 97 PasI CC'CwG_GG Cuts at: 1348 2518 1348 Size: 1170 5062 PciI A'CATG_T Cuts at: 5131 5131 Size: 6232 PflMI CCAn_nnn'nTGG Cuts at: 1108 2244 2572 2607 2839 3067 3213 1108 Size: 1136 328 35 232 228 146 4127 Fragments arranged by size: 4127 1136 328 232 228 146 35 PmeI GTTT'AAAC Cuts at: 3536 3536 Size: 6232 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4301 4333 5814 5846 4301 Size: 32 1481 32 4687 Fragments arranged by size: 4687 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2070 2207 3245 2070 Size: 137 1038 5057 Fragments arranged by size: 5057 1038 137 PshAI GACnn'nnGTC Cuts at: 1613 2075 1613 Size: 462 5770 PsiI TTA'TAA Cuts at: 3802 3802 Size: 6232 PspOMI G'GGCC_C Cuts at: 2247 3341 2247 Size: 1094 5138 PspXI vC'TCGA_Gb Cuts at: 3417 3417 Size: 6232 PstI C_TGCA'G Cuts at: 830 2115 2652 2889 3584 830 Size: 1285 537 237 695 3478 Fragments arranged by size: 3478 1285 695 537 237 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6232 PvuII CAG'CTG Cuts at: 2653 2890 3446 2653 Size: 237 556 5439 Fragments arranged by size: 5439 556 237 SacI G_AGCT'C Cuts at: 3234 3234 Size: 6232 SalI G'TCGA_C Cuts at: 1936 3574 1936 Size: 1638 4594 SanDI GG'GwC_CC Cuts at: 2070 2207 3245 2070 Size: 137 1038 5057 Fragments arranged by size: 5057 1038 137 SapI GCTCTTCn'nnn_ Cuts at: 3239 3239 Size: 6232 SbfI CC_TGCA'GG Cuts at: 3584 3584 Size: 6232 ScaI AGT'ACT Cuts at: 4480 6192 4480 Size: 1712 4520 SfcI C'TryA_G Cuts at: 826 1045 2111 2648 2885 3580 4715 5396 5587 Size: 219 1066 537 237 695 1135 681 191 Cuts at: 5587 826 Size: 1471 Fragments arranged by size: 1471 1135 1066 695 681 537 237 219 191 SfoI GGC'GCC Cuts at: 2443 2515 3050 2443 Size: 72 535 5625 Fragments arranged by size: 5625 535 72 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6232 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6232 SmaI CCC'GGG Cuts at: 1872 3559 1872 Size: 1687 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2711 3400 3417 3503 4336 5237 Size: 197 926 768 689 17 86 833 901 Cuts at: 5237 5499 820 Size: 262 1553 Fragments arranged by size: 1553 926 901 833 768 689 262 197 86 17 SnaBI TAC'GTA Cuts at: 493 4023 493 Size: 3530 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6232 SphI G_CATG'C Cuts at: 1132 3590 1132 Size: 2458 3774 SspI AAT'ATT Cuts at: 5 52 4156 5 Size: 47 4104 2081 Fragments arranged by size: 4104 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6232 StyI C'CwwG_G Cuts at: 513 1065 2422 3015 3212 3261 3671 513 Size: 552 1357 593 197 49 410 3074 Fragments arranged by size: 3074 1357 593 552 410 197 49 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4259 4276 4429 4614 5059 325 Size: 1255 2679 17 153 185 445 1498 Fragments arranged by size: 2679 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4478 6190 96 Size: 274 80 33 51 1443 2501 1712 138 Fragments arranged by size: 2501 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2979 3011 6192 6224 2979 Size: 32 3181 32 2987 Fragments arranged by size: 3181 2987 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6232 XbaI T'CTAG_A Cuts at: 3568 3568 Size: 6232 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2269 2903 2269 Size: 634 5598 XhoI C'TCGA_G Cuts at: 1943 2711 3417 1943 Size: 768 706 4758 Fragments arranged by size: 4758 768 706 XmaI C'CCGG_G Cuts at: 1870 3557 1870 Size: 1687 4545 XmnI GAAnn'nnTTC Cuts at: 4361 4361 Size: 6232 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4040 276 Size: 53 83 186 936 2506 2468 Fragments arranged by size: 2506 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI BstZ17I DraIII EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PsrI RsrII SacII SexAI SfiI SrfI SwaI