Restriction map for
pFN21AB0463
(Linear) (Six-base) MAPSORT of: KIBB0463.seq Check: 4544 from: 1 to: 1527
KIBB0463.orf
With 182 enzymes: SgfI *
October 7, 2009 14:14 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 509 1527
Size: 509 1018
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 751 799 1215 1527
Size: 751 48 416 312
Fragments arranged by size:
751 416 312 48
AflII C'TTAA_G
Cuts at: 0 1395 1527
Size: 1395 132
AlwNI CAG_nnn'CTG
Cuts at: 0 567 834 861 1527
Size: 567 267 27 666
Fragments arranged by size:
666 567 267 27
ApaI G_GGCC'C
Cuts at: 0 246 1340 1527
Size: 246 1094 187
Fragments arranged by size:
1094 246 187
AvaI C'yCGr_G
Cuts at: 0 670 706 1412 1527
Size: 670 36 706 115
Fragments arranged by size:
706 670 115 36
BanI G'GyrC_C
Cuts at: 0 223 436 465 508 1043 1527
Size: 223 213 29 43 535 484
Fragments arranged by size:
535 484 223 213 43 29
BanII G_rGCy'C
Cuts at: 0 246 679 1229 1340 1527
Size: 246 433 550 111 187
Fragments arranged by size:
550 433 246 187 111
BbeI G_GCGC'C
Cuts at: 0 440 512 1047 1527
Size: 440 72 535 480
Fragments arranged by size:
535 480 440 72
BbsI GAAGACnn'nnnn_
Cuts at: 0 996 1338 1527
Size: 996 342 189
Fragments arranged by size:
996 342 189
BbvCI CC'TCA_GC
Cuts at: 0 47 1527
Size: 47 1480
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 537 571 697 731 1527
Size: 537 34 126 34 796
Fragments arranged by size:
796 537 126 34 34
BclI T'GATC_A
Cuts at: 0 863 1527
Size: 863 664
BglI GCCn_nnn'nGGC
Cuts at: 0 377 743 1073 1527
Size: 377 366 330 454
Fragments arranged by size:
454 377 366 330
BglII A'GATC_T
Cuts at: 0 1433 1527
Size: 1433 94
BlpI GC'TnA_GC
Cuts at: 0 773 886 1527
Size: 773 113 641
Fragments arranged by size:
773 641 113
Bme1580I G_kGCm'C
Cuts at: 0 246 470 592 1340 1527
Size: 246 224 122 748 187
Fragments arranged by size:
748 246 224 187 122
BmgBI CAC'GTC
Cuts at: 0 176 1527
Size: 176 1351
BmrI ACTGGGnnnn_n'
Cuts at: 0 71 1527
Size: 71 1456
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 203 782 811 820 1141 1527
Size: 203 579 29 9 321 386
Fragments arranged by size:
579 386 321 203 29 9
Bpu10I CC'TnA_GC
Cuts at: 0 47 1527
Size: 47 1480
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1519 1527
Size: 1519 8
BsaI GGTCTCn'nnnn_
Cuts at: 0 1020 1527
Size: 1020 507
BsaAI yAC'GTr
Cuts at: 0 477 1527
Size: 477 1050
BsaHI Gr'CG_yC
Cuts at: 0 437 509 1044 1527
Size: 437 72 535 483
Fragments arranged by size:
535 483 437 72
BsaWI w'CCGG_w
Cuts at: 0 1451 1527
Size: 1451 76
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 623 653 974 1004 1527
Size: 623 30 321 30 523
Fragments arranged by size:
623 523 321 30 30
BseYI C'CCAG_C
Cuts at: 0 180 205 360 567 746 1371 1486 1527
Size: 180 25 155 207 179 625 115 41
Fragments arranged by size:
625 207 180 179 155 115 41 25
BsiEI CG_ry'CG
Cuts at: 0 875 1527
Size: 875 652
BsiHKAI G_wGCw'C
Cuts at: 0 1229 1527
Size: 1229 298
BsmI GAATG_Cn'
Cuts at: 0 548 1527
Size: 548 979
BsmBI CGTCTCn'nnnn_
Cuts at: 0 166 1152 1527
Size: 166 986 375
Fragments arranged by size:
986 375 166
Bsp1286I G_dGCh'C
Cuts at: 0 246 470 592 679 1229 1340 1527
Size: 246 224 122 87 550 111 187
Fragments arranged by size:
550 246 224 187 122 111 87
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 509 642 1527
Size: 509 133 885
Fragments arranged by size:
885 509 133
BsrBI CCG'CTC
Cuts at: 0 810 1035 1527
Size: 810 225 492
Fragments arranged by size:
810 492 225
BsrDI GCAATG_nn'
Cuts at: 0 866 1527
Size: 866 661
BsrFI r'CCGG_y
Cuts at: 0 851 1527
Size: 851 676
BstXI CCAn_nnnn'nTGG
Cuts at: 0 477 1263 1527
Size: 477 786 264
Fragments arranged by size:
786 477 264
BstYI r'GATC_y
Cuts at: 0 1433 1527
Size: 1433 94
Bsu36I CC'TnA_GG
Cuts at: 0 197 1527
Size: 197 1330
BtgI C'CryG_G
Cuts at: 0 251 266 1256 1527
Size: 251 15 990 271
Fragments arranged by size:
990 271 251 15
BtsI GCAGTG_nn'
Cuts at: 0 590 607 1527
Size: 590 17 920
Fragments arranged by size:
920 590 17
EaeI y'GGCC_r
Cuts at: 0 254 383 533 604 811 1157 1220 1527
Size: 254 129 150 71 207 346 63 307
Fragments arranged by size:
346 307 254 207 150 129 71 63
EarI CTCTTCn'nnn_
Cuts at: 0 1234 1527
Size: 1234 293
EcoICRI GAG'CTC
Cuts at: 0 1227 1527
Size: 1227 300
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 203 751 782 799 811 820 1141 1215
Size: 203 548 31 17 12 9 321 74
Cuts at: 1215 1527
Size: 312
Fragments arranged by size:
548 321 312 203 74 31 17 12
9
EcoO109I rG'GnC_Cy
Cuts at: 0 65 193 202 243 712 1240 1336 1337
Size: 65 128 9 41 469 528 96 1
Cuts at: 1337 1527
Size: 190
Fragments arranged by size:
528 469 190 128 96 65 41 9
1
HaeII r_GCGC'y
Cuts at: 0 440 512 738 1047 1527
Size: 440 72 226 309 480
Fragments arranged by size:
480 440 309 226 72
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 413 445 1041 1073 1092 1124 1420 1446
Size: 413 32 596 32 19 32 296 26
Cuts at: 1446 1452 1478 1527
Size: 6 26 49
Fragments arranged by size:
596 413 296 49 32 32 32 26
26 19 6
HincII GTy'rAC
Cuts at: 0 342 996 1527
Size: 342 654 531
Fragments arranged by size:
654 531 342
KasI G'GCGC_C
Cuts at: 0 436 508 1043 1527
Size: 436 72 535 484
Fragments arranged by size:
535 484 436 72
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 39 1301 1527
Size: 39 1262 226
Fragments arranged by size:
1262 226 39
MscI TGG'CCA
Cuts at: 0 256 385 535 606 1159 1527
Size: 256 129 150 71 553 368
Fragments arranged by size:
553 368 256 150 129 71
MslI CAynn'nnrTG
Cuts at: 0 475 1096 1261 1303 1527
Size: 475 621 165 42 224
Fragments arranged by size:
621 475 224 165 42
MspA1I CmG'CkG
Cuts at: 0 648 885 1441 1527
Size: 648 237 556 86
Fragments arranged by size:
648 556 237 86
NarI GG'CG_CC
Cuts at: 0 437 509 1044 1527
Size: 437 72 535 483
Fragments arranged by size:
535 483 437 72
NcoI C'CATG_G
Cuts at: 0 1256 1527
Size: 1256 271
NspI r_CATG'y
Cuts at: 0 1080 1527
Size: 1080 447
PasI CC'CwG_GG
Cuts at: 0 513 1527
Size: 513 1014
PflMI CCAn_nnn'nTGG
Cuts at: 0 239 567 602 834 1062 1208 1527
Size: 239 328 35 232 228 146 319
Fragments arranged by size:
328 319 239 232 228 146 35
PpuMI rG'GwC_Cy
Cuts at: 0 65 202 1240 1527
Size: 65 137 1038 287
Fragments arranged by size:
1038 287 137 65
PshAI GACnn'nnGTC
Cuts at: 0 70 1527
Size: 70 1457
PspOMI G'GGCC_C
Cuts at: 0 242 1336 1527
Size: 242 1094 191
Fragments arranged by size:
1094 242 191
PspXI vC'TCGA_Gb
Cuts at: 0 1412 1527
Size: 1412 115
PstI C_TGCA'G
Cuts at: 0 110 647 884 1527
Size: 110 537 237 643
Fragments arranged by size:
643 537 237 110
PvuII CAG'CTG
Cuts at: 0 648 885 1441 1527
Size: 648 237 556 86
Fragments arranged by size:
648 556 237 86
SacI G_AGCT'C
Cuts at: 0 1229 1527
Size: 1229 298
SanDI GG'GwC_CC
Cuts at: 0 65 202 1240 1527
Size: 65 137 1038 287
Fragments arranged by size:
1038 287 137 65
SapI GCTCTTCn'nnn_
Cuts at: 0 1234 1527
Size: 1234 293
SfcI C'TryA_G
Cuts at: 0 106 643 880 1527
Size: 106 537 237 647
Fragments arranged by size:
647 537 237 106
SfoI GGC'GCC
Cuts at: 0 438 510 1045 1527
Size: 438 72 535 482
Fragments arranged by size:
535 482 438 72
SmlI C'TyrA_G
Cuts at: 0 706 1395 1412 1498 1527
Size: 706 689 17 86 29
Fragments arranged by size:
706 689 86 29 17
StyI C'CwwG_G
Cuts at: 0 417 1010 1207 1256 1527
Size: 417 593 197 49 271
Fragments arranged by size:
593 417 271 197 49
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 974 1006 1527
Size: 974 32 521
Fragments arranged by size:
974 521 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 264 898 1527
Size: 264 634 629
Fragments arranged by size:
634 629 264
XhoI C'TCGA_G
Cuts at: 0 706 1412 1527
Size: 706 706 115
Fragments arranged by size:
706 706 115
Enzymes that do cut:
AarI AcuI AflII AlwNI ApaI AvaI BanI BanII
BbeI BbsI BbvCI BcgI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BsaI BsaAI
BsaHI BsaWI BsaXI BseYI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspMI BsrBI BsrDI BsrFI BstXI BstYI Bsu36I
BtgI BtsI EaeI EarI EcoICRI Eco57MI EcoO109I HaeII
Hin4I HincII KasI MmeI MscI MslI MspA1I NarI
NcoI NspI PasI PflMI PpuMI PshAI PspOMI PspXI
PstI PvuII SacI SanDI SapI SfcI SfoI SmlI
StyI TstI XcmI XhoI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflIII AgeI AhdI
AleI AlfI AloI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BciVI BfrBI BmtI BplI BsaBI
BseRI BsgI BsiWI BspEI BspHI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstZ17I BtgZI ClaI CspCI DraI
DraIII DrdI EagI EciI EcoNI EcoRI EcoRV FalI
FseI FspI FspAI HindIII HpaI KpnI MfeI MluI
NaeI NdeI NgoMIV NheI NotI NruI NsiI PacI
PciI PfoI PmeI PmlI PpiI PsiI PsrI PvuI
RsrII SacII SalI SbfI ScaI SexAI SfiI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI
SwaI TaqII TatI Tth111I XbaI XmaI XmnI ZraI