Restriction map for pFN21AB6459
 (Circular) (Six-base) MAPSORT of: pFN21AB6459.seq  Check: 2207  from: 1  to: 5659



pFN21AB6459  5659 bp

 With 182 enzymes: SgfI * 

                        April 15, 2010 09:32  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    3469     278 
   Size:         53      83     186     936    1933    2468

  Fragments arranged by size: 

                2468    1933     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    3002    1937 
   Size:       1065    4594
 
Acc65I G'GTAC_C

Cuts at:   2980    2980 
   Size:       5659
 
AclI AA'CG_TT

Cuts at:   1563    3786    4159    1563 
   Size:       2223     373    3063

  Fragments arranged by size: 

                3063    2223     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    3722    5106    5374    1632 
   Size:       2090    1384     268    1917

  Fragments arranged by size: 

                2090    1917    1384     268
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    5462
 
AflIII A'CryG_T

Cuts at:   1203    4558    5535    1203 
   Size:       3355     977    1327

  Fragments arranged by size: 

                3355    1327     977
 
AgeI A'CCGG_T

Cuts at:   4490    4490 
   Size:       5659
 
AhdI GACnn_n'nnGTC

Cuts at:   2458    4388    2458 
   Size:       1930    3729
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       5659
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       5659
 
AlwNI CAG_nnn'CTG

Cuts at:   2474    3402    4974    2474 
   Size:        928    1572    3159

  Fragments arranged by size: 

                3159    1572     928
 
ApaLI G'TGCA_C

Cuts at:   3716    4872    3716 
   Size:       1156    4503
 
ApoI r'AATT_y

Cuts at:   1493    2355    2388    2507    2773    2968    3194    3346    5455 
   Size:        862      33     119     266     195     226     152    2109

Cuts at:   5455    1493 
   Size:       1697

  Fragments arranged by size: 

                2109    1697     862     266     226     195     152     119
                  33
 
AseI AT'TA_AT

Cuts at:    160    1032    2486    2681    4213     160 
   Size:        872    1454     195    1532    1606

  Fragments arranged by size: 

                1606    1532    1454     872     195
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5659
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2984    1870 
   Size:         73    1041    4545

  Fragments arranged by size: 

                4545    1041      73
 
BamHI G'GATC_C

Cuts at:   2989    3356    2989 
   Size:        367    5292
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2980    4435     618 
   Size:        325     217     627    1193    1455    1842

  Fragments arranged by size: 

                1842    1455    1193     627     325     217
 
BanII G_rGCy'C

Cuts at:   1391    2194    2978    1391 
   Size:        803     784    4072

  Fragments arranged by size: 

                4072     803     784
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899     928 
   Size:        971    4688
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    2816    2850    3850    3884    1903 
   Size:         34     879      34    1000      34    3678

  Fragments arranged by size: 

                3678    1000     879      34      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3551    4761    3551 
   Size:       1210    4449
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       5659
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    4270     136 
   Size:        107     122      71     631     605    2598    1525

  Fragments arranged by size: 

                2598    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   5654    5654 
   Size:       5659
 
BlpI GC'TnA_GC

Cuts at:   3076    3076 
   Size:       5659
 
Bme1580I G_kGCm'C

Cuts at:   1790    3720    4876    1790 
   Size:       1930    1156    2573

  Fragments arranged by size: 

                2573    1930    1156
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       5659
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    2484    3390    4348 
   Size:        416      53      83     399      24    1067     906     958

Cuts at:   4348     442 
   Size:       1753

  Fragments arranged by size: 

                1753    1067     958     906     416     399      83      53
                  24
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5659
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    4319    1239 
   Size:       3080    2579
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   2084    2861    3784    4649    4947    2084 
   Size:        777     923     865     298    2796

  Fragments arranged by size: 

                2796     923     865     777     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    4322     882 
   Size:        617    2823    2219

  Fragments arranged by size: 

                2823    2219     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    3450     493 
   Size:        711    2246    2702

  Fragments arranged by size: 

                2702    2246     711
 
BsaBI GATnn'nnATC

Cuts at:   2913    5653    2913 
   Size:       2740    2919
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    3466    3848    4483     275 
   Size:         53      83     186     936    1933     382     635    1451

  Fragments arranged by size: 

                1933    1451     936     635     382     186      83      53
            
 
BsaWI w'CCGG_w

Cuts at:   4092    4490    4764    4911    5322    4092 
   Size:        398     274     147     411    4429

  Fragments arranged by size: 

                4429     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    1178 
   Size:         30     165      30    5434

  Fragments arranged by size: 

                5434     165      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2255    1186 
   Size:       1069    4590
 
BseYI C'CCAG_C

Cuts at:   2593    4862    5358    2593 
   Size:       2269     496    2894

  Fragments arranged by size: 

                2894    2269     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    2487    1163 
   Size:        573     751    4335

  Fragments arranged by size: 

                4335     751     573
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    3109    3870    4898    1540 
   Size:        275     186    1108     761    1028    2301

  Fragments arranged by size: 

                2301    1108    1028     761     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   2082    2194    2606    3720    3805    4876    2082 
   Size:        112     412    1114      85    1071    2865

  Fragments arranged by size: 

                2865    1114    1071     412     112      85
 
BsmI GAATG_Cn'

Cuts at:   3170    3263    3170 
   Size:         93    5566
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2082    2194    2606    2978    3720    3805    4876 
   Size:        399     292     112     412     372     742      85    1071

Cuts at:   4876    1391 
   Size:       2174

  Fragments arranged by size: 

                2174    1071     742     412     399     372     292     112
                  85
 
BspEI T'CCGG_A

Cuts at:   5322    5322 
   Size:       5659
 
BspHI T'CATG_A

Cuts at:   2220    2503    3548    5278    2220 
   Size:        283    1045    1730    2601

  Fragments arranged by size: 

                2601    1730    1045     283
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2173    3014     844 
   Size:       1329     841    3489

  Fragments arranged by size: 

                3489    1329     841
 
BsrBI CCG'CTC

Cuts at:   1394    1823    3546    1394 
   Size:        429    1723    3507

  Fragments arranged by size: 

                3507    1723     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    4154    4328      65 
   Size:       1183    2906     174    1396

  Fragments arranged by size: 

                2906    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    4303    4490    1700 
   Size:        110    2493     187    2869

  Fragments arranged by size: 

                2869    2493     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       5659
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5659
 
BssSI C'ACGA_G

Cuts at:   2235    3719    4731    2235 
   Size:       1484    1012    3163

  Fragments arranged by size: 

                3163    1484    1012
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       5659
 
BstBI TT'CG_AA

Cuts at:   4463    4463 
   Size:       5659
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       5659
 
BstYI r'GATC_y

Cuts at:   1972    2908    2989    3356    3741    3758    5210    5296    5308 
   Size:        936      81     367     385      17    1452      86      12

Cuts at:   5308    5654    1972 
   Size:        346    1977

  Fragments arranged by size: 

                1977    1452     936     385     367     346      86      81
                  17      12
 
BtgI C'CryG_G

Cuts at:    513    1065    2004     513 
   Size:        552     939    4168

  Fragments arranged by size: 

                4168     939     552
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    4920

  Fragments arranged by size: 

                4920     683      56
 
BtsI GCAGTG_nn'

Cuts at:   3175    3969    3989    3175 
   Size:        794      20    4845

  Fragments arranged by size: 

                4845     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5659
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564    2444    2479     529 
   Size:         35    1880      35    3709

  Fragments arranged by size: 

                3709    1880      35      35
 
DraI TTT'AAA

Cuts at:   2859    2963    2859 
   Size:        104    5555
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4666     809 
   Size:        507    3350    1802

  Fragments arranged by size: 

                3350    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2492    3106    3995 
   Size:         54    1425      50     275      18     662     614     889

Cuts at:   3995    5624       8 
   Size:       1629      43

  Fragments arranged by size: 

                1629    1425     889     662     614     275      54      50
                  43      18
 
EagI C'GGCC_G

Cuts at:   1537    1812    3106    1537 
   Size:        275    1294    4090

  Fragments arranged by size: 

                4090    1294     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    3589    1353 
   Size:       2236    3423
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    4246    4620    4766     387 
   Size:       1064     138    2657     374     146    1280

  Fragments arranged by size: 

                2657    1280    1064     374     146     138
 
EcoICRI GAG'CTC

Cuts at:   2192    2192 
   Size:       5659
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    3722    4319    5106    5374    1239 
   Size:        393    2090     597     787     268    1524

  Fragments arranged by size: 

                2090    1524     787     597     393     268
 
EcoRI G'AATT_C

Cuts at:   2968    3346    2968 
   Size:        378    5281
 
EcoRV GAT'ATC

Cuts at:   2936    2936 
   Size:       5659
 
FspI TGC'GCA

Cuts at:   4165    4165 
   Size:       5659
 
HaeII r_GCGC'y

Cuts at:   1678    4806    1678 
   Size:       3128    2531
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2218    2250    2444    2476    4300 
   Size:         32     135      32     646      32     194      32    1824

Cuts at:   4300    4332    4374    4406    1373 
   Size:         32      42      32    2626

  Fragments arranged by size: 

                2626    1824     646     194     135      42      32      32
                  32      32      32      32
 
HincII GTy'rAC

Cuts at:    669    1657    1938    3003    3249     669 
   Size:        988     281    1065     246    3079

  Fragments arranged by size: 

                3079    1065     988     281     246
 
HindIII A'AGCT_T

Cuts at:    748    2183    2417     748 
   Size:       1435     234    3990

  Fragments arranged by size: 

                3990    1435     234
 
HpaI GTT'AAC

Cuts at:   3249    3249 
   Size:       5659
 
KpnI G_GTAC'C

Cuts at:   2984    2984 
   Size:       5659
 
MfeI C'AATT_G

Cuts at:   3258    3258 
   Size:       5659
 
MluI A'CGCG_T

Cuts at:   5535    5535 
   Size:       5659
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    2616    4773    4957    1305 
   Size:       1311    2157     184    2007

  Fragments arranged by size: 

                2157    2007    1311     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832      10 
   Size:         54    1425     343    3837

  Fragments arranged by size: 

                3837    1425     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2225    2750    3617    3976    4135     518 
   Size:        648    1059     525     867     359     159    2042

  Fragments arranged by size: 

                2042    1059     867     648     525     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    3075    3752    4900    5145    1606 
   Size:       1469     677    1148     245    2120

  Fragments arranged by size: 

                2120    1469    1148     677     245
 
NcoI C'CATG_G

Cuts at:    513    1065    2004     513 
   Size:        552     939    4168

  Fragments arranged by size: 

                4168     939     552
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       5659
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5659
 
NotI GC'GGCC_GC

Cuts at:   3106    3106 
   Size:       5659
 
NspI r_CATG'y

Cuts at:   1132    1229    3017    4562    1132 
   Size:         97    1788    1545    2229

  Fragments arranged by size: 

                2229    1788    1545      97
 
PasI CC'CwG_GG

Cuts at:   1348    2403    1348 
   Size:       1055    4604
 
PciI A'CATG_T

Cuts at:   4558    4558 
   Size:       5659
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    2795    1108 
   Size:       1687    3972
 
PmeI GTTT'AAAC

Cuts at:   2963    2963 
   Size:       5659
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3728    3760    5241    5273    3728 
   Size:         32    1481      32    4114

  Fragments arranged by size: 

                4114    1481      32      32
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5659
 
PsiI TTA'TAA

Cuts at:   2619    3229    2619 
   Size:        610    5049
 
PstI C_TGCA'G

Cuts at:    830    2170    3011     830 
   Size:       1340     841    3478

  Fragments arranged by size: 

                3478    1340     841
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5659
 
SacI G_AGCT'C

Cuts at:   2194    2194 
   Size:       5659
 
SalI G'TCGA_C

Cuts at:   1936    3001    1936 
   Size:       1065    4594
 
SbfI CC_TGCA'GG

Cuts at:   3011    3011 
   Size:       5659
 
ScaI AGT'ACT

Cuts at:   3907    5619    3907 
   Size:       1712    3947
 
SfcI C'TryA_G

Cuts at:    826    1045    2166    3007    4142    4823    5014     826 
   Size:        219    1121     841    1135     681     191    1471

  Fragments arranged by size: 

                1471    1135    1121     841     681     219     191
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5659
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5659
 
SmaI CCC'GGG

Cuts at:   1872    2986    1872 
   Size:       1114    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    2099    2876    3763    4664    4926     820 
   Size:        197     926     156     777     887     901     262    1553

  Fragments arranged by size: 

                1553     926     901     887     777     262     197     156
            
 
SnaBI TAC'GTA

Cuts at:    493    3450     493 
   Size:       2957    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5659
 
SphI G_CATG'C

Cuts at:   1132    3017    1132 
   Size:       1885    3774
 
SspI AAT'ATT

Cuts at:      5      52    2924    3583       5 
   Size:         47    2872     659    2081

  Fragments arranged by size: 

                2872    2081     659      47
 
StuI AGG'CCT

Cuts at:   1517    2812    1517 
   Size:       1295    4364
 
StyI C'CwwG_G

Cuts at:    513    1065    2004    2669    3098     513 
   Size:        552     939     665     429    3074

  Fragments arranged by size: 

                3074     939     665     552     429
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    3686    3703    3856    4041    4486     325 
   Size:       1255    2106      17     153     185     445    1498

  Fragments arranged by size: 

                2106    1498    1255     445     185     153      17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    2376    2659    3905 
   Size:        274      80      33      51    1443     399     283    1246

Cuts at:   3905    5617      96 
   Size:       1712     138

  Fragments arranged by size: 

                1712    1443    1246     399     283     274     138      80
                  51      33
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   5619    5651    5619 
   Size:         32    5627
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       5659
 
XbaI T'CTAG_A

Cuts at:   2995    2995 
   Size:       5659
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   2138    2138 
   Size:       5659
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5659
 
XmaI C'CCGG_G

Cuts at:   1870    2984    1870 
   Size:       1114    4545
 
XmnI GAAnn'nnTTC

Cuts at:   2719    3788    2719 
   Size:       1069    4590
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    3467     276 
   Size:         53      83     186     936    1933    2468

  Fragments arranged by size: 

                2468    1933     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI    AsiSI
     AvaI    BamHI     BanI    BanII     BbsI     BcgI    BciVI     BclI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BpmI
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstYI     BtgI    BtgZI     BtsI     ClaI    CspCI
     DraI     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI
    EcoRI    EcoRV     FspI    HaeII    Hin4I   HincII  HindIII     HpaI
     KpnI     MfeI     MluI     MmeI     MscI     MslI   MspA1I     NcoI
     NdeI     NheI     NotI     NspI     PasI     PciI    PflMI     PmeI
     PpiI    PshAI     PsiI     PstI     PvuI     SacI     SalI     SbfI
     ScaI     SfcI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SspI     StuI     StyI    TaqII     TatI     TstI  Tth111I
     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     AloI     ApaI     AscI    AvrII     BaeI     BbeI
    BbvCI    BfrBI     BplI   Bpu10I    BsiWI    BsmBI    BstXI  BstZ17I
   Bsu36I   DraIII    EcoNI EcoO109I     FalI     FseI    FspAI     KasI
     NaeI     NarI   NgoMIV     NruI     NsiI     PacI     PfoI     PmlI
    PpuMI   PspOMI    PspXI     PsrI    PvuII    RsrII    SacII    SanDI
     SapI    SexAI     SfiI     SfoI     SrfI     SwaI
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