Restriction map for
pFN21AB0421
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# NO CUT ENZYMES ( 124 enzymes) #
##################################
AatII Acc65I AclI AcsI AcyI
AflII AflIII AgeI Alw44I Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AviII AvrII BanI
BbeI BbrPI BcgI BciVI BclI
BfrI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BsaHI BsgI BsiEI
BsiWI BsmI BspDI BspHI BspLU11I
BspMI BsrDI Bst1107I BstAPI BstBI
BstZ17I CelII ClaI CpoI DraI
DrdI DsaI EagI EclXI Eco47III
Eco52I EcoNI EcoRI EcoRV EcoT22I
FbaI FseI FspI HaeII Hin1I
HincII HindII HindIII HpaI KasI
KpnI KspI MamI MfeI MluI
MseI MunI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SapI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI SspI
StuI SwaI Tru9I XbaI XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AccI 976 GTAGAC GT^[AC][GT]AC
AccIII 244 TCCGGA T^CCGGA
AhdI 581 GACCCCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 1139 GGGCCC GGGCC^C
AspEI 581 GACCCCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 796 GAGCAC G[AT]GC[AT]^C
BamHI 137 GGATCC G^GATCC
BbvCI 1102 CCTCAGC CCTCAGC(-5/-2)
BpmI 779 CTCCAG CTGGAG(16/14)
BsaWI 244 TCCGGA [AT]^CCGG[AT]
BseAI 244 TCCGGA T^CCGGA
BsiHKAI 796 GAGCAC G[AT]GC[AT]^C
BsmBI 1209 GAGACG CGTCTC(1/5)
Bsp1407I 729 TGTACA T^GTACA
BspEI 244 TCCGGA T^CCGGA
BsrBI 28 GAGCGG CCGCTC(-3/-3)
BsrFI 47 ACCGGC [GA]^CCGG[CT]
BsrGI 729 TGTACA T^GTACA
BssHII 161 GCGCGC G^CGCGC
BstEII 1095 GGTGACC G^GT[ACGT]ACC
BstF5I 945 CATCC GGATG(2/0)
BstPI 1095 GGTGACC G^GT[ACGT]ACC
BstXI 746 CCAAGGAATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 137 GGATCC [GA]^GATC[CT]
Cfr10I 47 ACCGGC [GA]^CCGG[CT]
DraII 269 GGGTCCT [GA]G^G[ACGT]CC[CT]
DraIII 722 CACTCCGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 581 GACCCCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 484 GAAGAG CTCTTC(1/4)
EcoO109I 269 GGGTCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 1095 GGTGACC G^GT[ACGT]ACC
FauI 402 GCGGG CCCGC(4/6)
FokI 945 CATCC GGATG(9/13)
HgaI 176 GCGTC GACGC(5/10)
Ksp632I 484 GAAGAG CTCTTC(1/4)
MaeII 143 ACGT A^CGT
MflI 137 GGATCC [GA]^GATC[CT]
MroI 244 TCCGGA T^CCGGA
MslI 387 CATGGAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
PflMI 543 CCAAGATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 269 GGGTCCT [GA]G^G[AT]CC[CT]
PstI 1156 CTGCAG CTGCA^G
SbfI 1155 CCTGCAGG CCTGCA^GG
SfaNI 607 GATGC GCATC(5/9)
Sse8387I 1155 CCTGCAGG CCTGCA^GG
SspBI 729 TGTACA T^GTACA
TaqI 299 TCGA T^CGA
TspRI 261 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 299 TCGA T^CGA
Van91I 543 CCAAGATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 137 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccII 93 CGCG CG^CG
162 CGCG
AfaI 553 GTAC GT^AC
730 GTAC
AlwNI 522 CAGTTCCTG CAG[ACGT][ACGT][ACGT]^CTG
1061 CAGCCTCTG
AocI 11 CCTCAGG CC^T[ACGT]AGG
478 CCTGAGG
AspI 172 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
597 GACGGTGTC
AvaI 133 CTCGGG C^[CT]CG[GA]G
377 CTCGGG
BfaI 217 CTAG C^TAG
697 CTAG
BglI 24 GCCGGAGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1216 GCCCAGGAGGC
BsoBI 133 CTCGGG C^[CT]CG[GA]G
377 CTCGGG
BstUI 93 CGCG CG^CG
162 CGCG
Bsu36I 11 CCTCAGG CC^T[ACGT]AGG
478 CCTGAGG
DpnII 138 GATC ^GATC
256 GATC
Eco81I 11 CCTCAGG CC^T[ACGT]AGG
478 CCTGAGG
EcoT14I 746 CCAAGG C^C[AT][AT]GG
1037 CCAAGG
MaeI 217 CTAG C^TAG
697 CTAG
MboI 138 GATC ^GATC
256 GATC
MvnI 93 CGCG CG^CG
162 CGCG
NdeII 138 GATC ^GATC
256 GATC
PflFI 172 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
597 GACGGTGTC
RsaI 553 GTAC GT^AC
730 GTAC
Sau3AI 138 GATC ^GATC
256 GATC
SfcI 330 CTGTAG C^T[GA][CT]AG
1156 CTGCAG
SmlI 424 CTCAAG C^T[CT][GA]AG
1000 CTTGAG
StyI 746 CCAAGG C^C[AT][AT]GG
1037 CCAAGG
Tsp509I 316 AATT ^AATT
752 AATT
Tth111I 172 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
597 GACGGTGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 57 enzymes) #
#########################################
AciI AluI AlwI AvaII BalI
BanII BbsI BbvI BcnI BmyI
BpuAI BsaI BsaJI BseRI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrI
BssKI BssSI BstNI Cac8I CfoI
Cfr13I DdeI EaeI EcoRII Fnu4HI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI MaeIII MboII
MluNI MnlI MscI MspA1I MspI
MvaI MwoI NciI NlaIII NlaIV
PleI PvuII Sau96I ScrFI TfiI
TseI Tsp45I