(Circular) (Six-base) MAPSORT of: pFN21AB0421.seq Check: 4048 from: 1 to: 5935 pFN21AB0421 5935 bp With 182 enzymes: SgfI * April 15, 2010 09:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3745 278 Size: 53 83 186 936 2209 2468 Fragments arranged by size: 2468 2209 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2982 3278 1937 Size: 1045 296 4594 Fragments arranged by size: 4594 1045 296 Acc65I G'GTAC_C Cuts at: 3256 3256 Size: 5935 AclI AA'CG_TT Cuts at: 1563 4062 4435 1563 Size: 2499 373 3063 Fragments arranged by size: 3063 2499 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2509 3051 3998 5382 5650 1632 Size: 877 542 947 1384 268 1917 Fragments arranged by size: 1917 1384 947 877 542 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5738 AflIII A'CryG_T Cuts at: 1203 4834 5811 1203 Size: 3631 977 1327 Fragments arranged by size: 3631 1327 977 AgeI A'CCGG_T Cuts at: 4766 4766 Size: 5935 AhdI GACnn_n'nnGTC Cuts at: 2591 4664 2591 Size: 2073 3862 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5935 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5935 AlwNI CAG_nnn'CTG Cuts at: 2532 3071 3678 5250 2532 Size: 539 607 1572 3217 Fragments arranged by size: 3217 1572 607 539 ApaI G_GGCC'C Cuts at: 3148 3148 Size: 5935 ApaLI G'TGCA_C Cuts at: 3992 5148 3992 Size: 1156 4779 ApoI r'AATT_y Cuts at: 1493 3244 3470 3622 5731 1493 Size: 1751 226 152 2109 1697 Fragments arranged by size: 2109 1751 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4489 160 Size: 872 3457 1606 Fragments arranged by size: 3457 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5935 AvaI C'yCGr_G Cuts at: 1870 1943 2138 2382 3260 1870 Size: 73 195 244 878 4545 Fragments arranged by size: 4545 878 244 195 73 BamHI G'GATC_C Cuts at: 2142 3265 3632 2142 Size: 1123 367 4445 Fragments arranged by size: 4445 1123 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3256 4711 618 Size: 325 217 627 1469 1455 1842 Fragments arranged by size: 1842 1469 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2229 2517 3148 3223 3254 1391 Size: 838 288 631 75 31 4072 Fragments arranged by size: 4072 838 631 288 75 31 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2143 2844 3039 3129 928 Size: 971 244 701 195 90 3734 Fragments arranged by size: 3734 971 701 244 195 90 BbvCI CC'TCA_GC Cuts at: 3108 3108 Size: 5935 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4126 4160 1903 Size: 34 2189 34 3678 Fragments arranged by size: 3678 2189 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3827 5037 3827 Size: 1210 4725 BclI T'GATC_A Cuts at: 1548 1548 Size: 5935 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2035 3227 4546 Size: 107 122 71 631 605 363 1192 1319 Cuts at: 4546 136 Size: 1525 Fragments arranged by size: 1525 1319 1192 631 605 363 122 107 71 BglII A'GATC_T Cuts at: 5930 5930 Size: 5935 BlpI GC'TnA_GC Cuts at: 3352 3352 Size: 5935 Bme1580I G_kGCm'C Cuts at: 1790 3148 3996 5152 1790 Size: 1358 848 1156 2573 Fragments arranged by size: 2573 1358 1156 848 BmgBI CAC'GTC Cuts at: 1318 2149 1318 Size: 831 5104 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2833 2842 3666 Size: 416 53 83 399 24 1416 9 824 Cuts at: 3666 4624 442 Size: 958 1753 Fragments arranged by size: 1753 1416 958 824 416 399 83 53 24 9 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5935 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2032 2032 Size: 5935 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2769 4595 1239 Size: 1530 1826 2579 Fragments arranged by size: 2579 1826 1530 Bpu10I CC'TnA_GC Cuts at: 3108 3108 Size: 5935 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2414 3026 4060 4925 5223 2414 Size: 612 1034 865 298 3126 Fragments arranged by size: 3126 1034 865 612 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2385 2578 2599 2629 2952 4598 882 Size: 617 886 193 21 30 323 1646 2219 Fragments arranged by size: 2219 1646 886 617 323 193 30 21 BsaAI yAC'GTr Cuts at: 493 1204 3726 493 Size: 711 2522 2702 Fragments arranged by size: 2702 2522 711 BsaBI GATnn'nnATC Cuts at: 5929 5929 Size: 5935 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3742 4124 4759 275 Size: 53 83 186 936 2209 382 635 1451 Fragments arranged by size: 2209 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2249 4368 4766 5040 5187 5598 2249 Size: 2119 398 274 147 411 2586 Fragments arranged by size: 2586 2119 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5710 Fragments arranged by size: 5710 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2108 2457 2732 1186 Size: 922 349 275 4389 Fragments arranged by size: 4389 922 349 275 BseYI C'CCAG_C Cuts at: 2363 3086 5138 5634 2363 Size: 723 2052 496 2664 Fragments arranged by size: 2664 2052 723 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5362 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3385 4146 5174 1540 Size: 275 186 1384 761 1028 2301 Fragments arranged by size: 2301 1384 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2805 3996 4081 5152 2805 Size: 1191 85 1071 3588 Fragments arranged by size: 3588 1191 1071 85 BsmI GAATG_Cn' Cuts at: 3446 3539 3446 Size: 93 5842 BsmBI CGTCTCn'nnnn_ Cuts at: 3208 3208 Size: 5935 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2229 2517 2805 3148 3223 3254 3996 Size: 399 439 288 288 343 75 31 742 Cuts at: 3996 4081 5152 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 439 399 343 288 288 85 75 31 BspEI T'CCGG_A Cuts at: 2249 5598 2249 Size: 3349 2586 BspHI T'CATG_A Cuts at: 3824 5554 3824 Size: 1730 4205 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3290 844 Size: 2446 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2035 3822 1394 Size: 429 212 1787 3507 Fragments arranged by size: 3507 1787 429 212 BsrDI GCAATG_nn' Cuts at: 65 1248 4430 4604 65 Size: 1183 3182 174 1396 Fragments arranged by size: 3182 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2052 4579 4766 1700 Size: 110 242 2527 187 2869 Fragments arranged by size: 2869 2527 242 187 110 BsrGI T'GTAC_A Cuts at: 96 2734 96 Size: 2638 3297 BssHII G'CGCG_C Cuts at: 1925 2166 1925 Size: 241 5694 BssSI C'ACGA_G Cuts at: 2081 2228 2449 3995 5007 2081 Size: 147 221 1546 1012 3009 Fragments arranged by size: 3009 1546 1012 221 147 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5935 BstBI TT'CG_AA Cuts at: 4739 4739 Size: 5935 BstEII G'GTnAC_C Cuts at: 1184 3100 1184 Size: 1916 4019 BstXI CCAn_nnnn'nTGG Cuts at: 2758 2758 Size: 5935 BstYI r'GATC_y Cuts at: 1972 2142 3265 3632 4017 4034 5486 5572 5584 Size: 170 1123 367 385 17 1452 86 12 Cuts at: 5584 5930 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1123 385 367 346 170 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2017 2484 2017 Size: 467 5468 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4444 Fragments arranged by size: 4444 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2678 487 Size: 683 56 1452 3744 Fragments arranged by size: 3744 1452 683 56 BtsI GCAGTG_nn' Cuts at: 3451 4245 4265 3451 Size: 794 20 5121 Fragments arranged by size: 5121 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5935 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2657 2692 529 Size: 35 2093 35 3772 Fragments arranged by size: 3772 2093 35 35 DraI TTT'AAA Cuts at: 3239 3239 Size: 5935 DraIII CAC_nnn'GTG Cuts at: 2732 2732 Size: 5935 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4942 809 Size: 507 3626 1802 Fragments arranged by size: 3626 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2503 2534 2748 Size: 54 1425 50 275 18 673 31 214 Cuts at: 2748 2964 3382 4271 5900 8 Size: 216 418 889 1629 43 Fragments arranged by size: 1629 1425 889 673 418 275 216 214 54 50 43 31 18 EagI C'GGCC_G Cuts at: 1537 1812 3382 1537 Size: 275 1570 4090 Fragments arranged by size: 4090 1570 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2484 3865 1353 Size: 1131 1381 3423 Fragments arranged by size: 3423 1381 1131 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4522 4896 5042 387 Size: 1064 138 2933 374 146 1280 Fragments arranged by size: 2933 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2509 2769 3051 3998 4595 5382 5650 Size: 393 877 260 282 947 597 787 268 Cuts at: 5650 1239 Size: 1524 Fragments arranged by size: 1524 947 877 787 597 393 282 268 260 EcoO109I rG'GnC_Cy Cuts at: 2275 2275 Size: 5935 EcoRI G'AATT_C Cuts at: 3244 3622 3244 Size: 378 5557 FspI TGC'GCA Cuts at: 4441 4441 Size: 5935 HaeII r_GCGC'y Cuts at: 1678 5082 1678 Size: 3404 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2577 2609 4576 4608 4650 Size: 32 135 32 1005 32 1967 32 42 Cuts at: 4650 4682 1373 Size: 32 2626 Fragments arranged by size: 2626 1967 1005 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3279 3525 669 Size: 988 281 1341 246 3079 Fragments arranged by size: 3079 1341 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5935 HpaI GTT'AAC Cuts at: 3525 3525 Size: 5935 KpnI G_GTAC'C Cuts at: 3260 3260 Size: 5935 MfeI C'AATT_G Cuts at: 3534 3534 Size: 5935 MluI A'CGCG_T Cuts at: 5811 5811 Size: 5935 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2310 5049 5233 1305 Size: 1005 2739 184 2007 Fragments arranged by size: 2739 2007 1005 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2536 2750 2966 10 Size: 54 1425 343 704 214 216 2979 Fragments arranged by size: 2979 1425 704 343 216 214 54 MslI CAynn'nnrTG Cuts at: 518 1166 2396 3893 4252 4411 518 Size: 648 1230 1497 359 159 2042 Fragments arranged by size: 2042 1497 1230 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2163 2743 2789 3351 4028 5176 5421 1606 Size: 557 580 46 562 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 580 562 557 245 46 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4444 Fragments arranged by size: 4444 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5935 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5935 NotI GC'GGCC_GC Cuts at: 3382 3382 Size: 5935 NspI r_CATG'y Cuts at: 1132 1229 3293 4838 1132 Size: 97 2064 1545 2229 Fragments arranged by size: 2229 2064 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5935 PciI A'CATG_T Cuts at: 4834 4834 Size: 5935 PflMI CCAn_nnn'nTGG Cuts at: 1108 2554 1108 Size: 1446 4489 PfoI T'CCnGG_A Cuts at: 2256 2256 Size: 5935 PmeI GTTT'AAAC Cuts at: 3239 3239 Size: 5935 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4004 4036 5517 5549 4004 Size: 32 1481 32 4390 Fragments arranged by size: 4390 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2275 2275 Size: 5935 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5935 PsiI TTA'TAA Cuts at: 3505 3505 Size: 5935 PspOMI G'GGCC_C Cuts at: 3144 3144 Size: 5935 PstI C_TGCA'G Cuts at: 830 3165 3287 830 Size: 2335 122 3478 Fragments arranged by size: 3478 2335 122 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5935 PvuII CAG'CTG Cuts at: 2163 2743 2789 2163 Size: 580 46 5309 Fragments arranged by size: 5309 580 46 SalI G'TCGA_C Cuts at: 1936 3277 1936 Size: 1341 4594 SbfI CC_TGCA'GG Cuts at: 3165 3287 3165 Size: 122 5813 ScaI AGT'ACT Cuts at: 4183 5895 4183 Size: 1712 4223 SfcI C'TryA_G Cuts at: 826 1045 2335 3161 3283 4418 5099 5290 826 Size: 219 1290 826 122 1135 681 191 1471 Fragments arranged by size: 1471 1290 1135 826 681 219 191 122 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5935 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5935 SmaI CCC'GGG Cuts at: 1872 3262 1872 Size: 1390 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2429 3005 4039 4940 5202 820 Size: 197 926 486 576 1034 901 262 1553 Fragments arranged by size: 1553 1034 926 901 576 486 262 197 SnaBI TAC'GTA Cuts at: 493 3726 493 Size: 3233 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5935 SphI G_CATG'C Cuts at: 1132 3293 1132 Size: 2161 3774 SspI AAT'ATT Cuts at: 5 52 3859 5 Size: 47 3807 2081 Fragments arranged by size: 3807 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5935 StyI C'CwwG_G Cuts at: 513 1065 2004 2751 3042 3374 513 Size: 552 939 747 291 332 3074 Fragments arranged by size: 3074 939 747 552 332 291 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2933 3962 3979 4132 4317 4762 325 Size: 1255 1353 1029 17 153 185 445 1498 Fragments arranged by size: 1498 1353 1255 1029 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2734 4181 5893 Size: 274 80 33 51 1443 757 1447 1712 Cuts at: 5893 96 Size: 138 Fragments arranged by size: 1712 1447 1443 757 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2197 2229 2440 2472 2634 2666 5895 5927 2197 Size: 32 211 32 162 32 3229 32 2205 Fragments arranged by size: 3229 2205 211 162 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2180 2605 1316 Size: 864 425 4646 Fragments arranged by size: 4646 864 425 XbaI T'CTAG_A Cuts at: 3271 3271 Size: 5935 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5935 XmaI C'CCGG_G Cuts at: 1870 3260 1870 Size: 1390 4545 XmnI GAAnn'nnTTC Cuts at: 4064 4064 Size: 5935 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3743 276 Size: 53 83 186 936 2209 2468 Fragments arranged by size: 2468 2209 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI