Restriction map for pFN21AB0421
 (Circular) (Six-base) MAPSORT of: pFN21AB0421.seq  Check: 4048  from: 1  to: 5935



pFN21AB0421  5935 bp

 With 182 enzymes: SgfI * 

                        April 15, 2010 09:32  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    3745     278 
   Size:         53      83     186     936    2209    2468

  Fragments arranged by size: 

                2468    2209     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2982    3278    1937 
   Size:       1045     296    4594

  Fragments arranged by size: 

                4594    1045     296
 
Acc65I G'GTAC_C

Cuts at:   3256    3256 
   Size:       5935
 
AclI AA'CG_TT

Cuts at:   1563    4062    4435    1563 
   Size:       2499     373    3063

  Fragments arranged by size: 

                3063    2499     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2509    3051    3998    5382    5650    1632 
   Size:        877     542     947    1384     268    1917

  Fragments arranged by size: 

                1917    1384     947     877     542     268
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    5738
 
AflIII A'CryG_T

Cuts at:   1203    4834    5811    1203 
   Size:       3631     977    1327

  Fragments arranged by size: 

                3631    1327     977
 
AgeI A'CCGG_T

Cuts at:   4766    4766 
   Size:       5935
 
AhdI GACnn_n'nnGTC

Cuts at:   2591    4664    2591 
   Size:       2073    3862
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       5935
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       5935
 
AlwNI CAG_nnn'CTG

Cuts at:   2532    3071    3678    5250    2532 
   Size:        539     607    1572    3217

  Fragments arranged by size: 

                3217    1572     607     539
 
ApaI G_GGCC'C

Cuts at:   3148    3148 
   Size:       5935
 
ApaLI G'TGCA_C

Cuts at:   3992    5148    3992 
   Size:       1156    4779
 
ApoI r'AATT_y

Cuts at:   1493    3244    3470    3622    5731    1493 
   Size:       1751     226     152    2109    1697

  Fragments arranged by size: 

                2109    1751    1697     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    4489     160 
   Size:        872    3457    1606

  Fragments arranged by size: 

                3457    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5935
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2138    2382    3260    1870 
   Size:         73     195     244     878    4545

  Fragments arranged by size: 

                4545     878     244     195      73
 
BamHI G'GATC_C

Cuts at:   2142    3265    3632    2142 
   Size:       1123     367    4445

  Fragments arranged by size: 

                4445    1123     367
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    3256    4711     618 
   Size:        325     217     627    1469    1455    1842

  Fragments arranged by size: 

                1842    1469    1455     627     325     217
 
BanII G_rGCy'C

Cuts at:   1391    2229    2517    3148    3223    3254    1391 
   Size:        838     288     631      75      31    4072

  Fragments arranged by size: 

                4072     838     631     288      75      31
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    2143    2844    3039    3129     928 
   Size:        971     244     701     195      90    3734

  Fragments arranged by size: 

                3734     971     701     244     195      90
 
BbvCI CC'TCA_GC

Cuts at:   3108    3108 
   Size:       5935
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    4126    4160    1903 
   Size:         34    2189      34    3678

  Fragments arranged by size: 

                3678    2189      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3827    5037    3827 
   Size:       1210    4725
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       5935
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    2035    3227    4546 
   Size:        107     122      71     631     605     363    1192    1319

Cuts at:   4546     136 
   Size:       1525

  Fragments arranged by size: 

                1525    1319    1192     631     605     363     122     107
                  71
 
BglII A'GATC_T

Cuts at:   5930    5930 
   Size:       5935
 
BlpI GC'TnA_GC

Cuts at:   3352    3352 
   Size:       5935
 
Bme1580I G_kGCm'C

Cuts at:   1790    3148    3996    5152    1790 
   Size:       1358     848    1156    2573

  Fragments arranged by size: 

                2573    1358    1156     848
 
BmgBI CAC'GTC

Cuts at:   1318    2149    1318 
   Size:        831    5104
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    2833    2842    3666 
   Size:        416      53      83     399      24    1416       9     824

Cuts at:   3666    4624     442 
   Size:        958    1753

  Fragments arranged by size: 

                1753    1416     958     824     416     399      83      53
                  24       9
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5935
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2032    2032 
   Size:       5935
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2769    4595    1239 
   Size:       1530    1826    2579

  Fragments arranged by size: 

                2579    1826    1530
 
Bpu10I CC'TnA_GC

Cuts at:   3108    3108 
   Size:       5935
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   2414    3026    4060    4925    5223    2414 
   Size:        612    1034     865     298    3126

  Fragments arranged by size: 

                3126    1034     865     612     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    2385    2578    2599    2629    2952    4598     882 
   Size:        617     886     193      21      30     323    1646    2219

  Fragments arranged by size: 

                2219    1646     886     617     323     193      30      21
            
 
BsaAI yAC'GTr

Cuts at:    493    1204    3726     493 
   Size:        711    2522    2702

  Fragments arranged by size: 

                2702    2522     711
 
BsaBI GATnn'nnATC

Cuts at:   5929    5929 
   Size:       5935
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    3742    4124    4759     275 
   Size:         53      83     186     936    2209     382     635    1451

  Fragments arranged by size: 

                2209    1451     936     635     382     186      83      53
            
 
BsaWI w'CCGG_w

Cuts at:   2249    4368    4766    5040    5187    5598    2249 
   Size:       2119     398     274     147     411    2586

  Fragments arranged by size: 

                2586    2119     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    1178 
   Size:         30     165      30    5710

  Fragments arranged by size: 

                5710     165      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2108    2457    2732    1186 
   Size:        922     349     275    4389

  Fragments arranged by size: 

                4389     922     349     275
 
BseYI C'CCAG_C

Cuts at:   2363    3086    5138    5634    2363 
   Size:        723    2052     496    2664

  Fragments arranged by size: 

                2664    2052     723     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    1163 
   Size:        573    5362
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    3385    4146    5174    1540 
   Size:        275     186    1384     761    1028    2301

  Fragments arranged by size: 

                2301    1384    1028     761     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   2805    3996    4081    5152    2805 
   Size:       1191      85    1071    3588

  Fragments arranged by size: 

                3588    1191    1071      85
 
BsmI GAATG_Cn'

Cuts at:   3446    3539    3446 
   Size:         93    5842
 
BsmBI CGTCTCn'nnnn_

Cuts at:   3208    3208 
   Size:       5935
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2229    2517    2805    3148    3223    3254    3996 
   Size:        399     439     288     288     343      75      31     742

Cuts at:   3996    4081    5152    1391 
   Size:         85    1071    2174

  Fragments arranged by size: 

                2174    1071     742     439     399     343     288     288
                  85      75      31
 
BspEI T'CCGG_A

Cuts at:   2249    5598    2249 
   Size:       3349    2586
 
BspHI T'CATG_A

Cuts at:   3824    5554    3824 
   Size:       1730    4205
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    3290     844 
   Size:       2446    3489
 
BsrBI CCG'CTC

Cuts at:   1394    1823    2035    3822    1394 
   Size:        429     212    1787    3507

  Fragments arranged by size: 

                3507    1787     429     212
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    4430    4604      65 
   Size:       1183    3182     174    1396

  Fragments arranged by size: 

                3182    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    2052    4579    4766    1700 
   Size:        110     242    2527     187    2869

  Fragments arranged by size: 

                2869    2527     242     187     110
 
BsrGI T'GTAC_A

Cuts at:     96    2734      96 
   Size:       2638    3297
 
BssHII G'CGCG_C

Cuts at:   1925    2166    1925 
   Size:        241    5694
 
BssSI C'ACGA_G

Cuts at:   2081    2228    2449    3995    5007    2081 
   Size:        147     221    1546    1012    3009

  Fragments arranged by size: 

                3009    1546    1012     221     147
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       5935
 
BstBI TT'CG_AA

Cuts at:   4739    4739 
   Size:       5935
 
BstEII G'GTnAC_C

Cuts at:   1184    3100    1184 
   Size:       1916    4019
 
BstXI CCAn_nnnn'nTGG

Cuts at:   2758    2758 
   Size:       5935
 
BstYI r'GATC_y

Cuts at:   1972    2142    3265    3632    4017    4034    5486    5572    5584 
   Size:        170    1123     367     385      17    1452      86      12

Cuts at:   5584    5930    1972 
   Size:        346    1977

  Fragments arranged by size: 

                1977    1452    1123     385     367     346     170      86
                  17      12
 
Bsu36I CC'TnA_GG

Cuts at:   2017    2484    2017 
   Size:        467    5468
 
BtgI C'CryG_G

Cuts at:    513    1065    2004     513 
   Size:        552     939    4444

  Fragments arranged by size: 

                4444     939     552
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226    2678     487 
   Size:        683      56    1452    3744

  Fragments arranged by size: 

                3744    1452     683      56
 
BtsI GCAGTG_nn'

Cuts at:   3451    4245    4265    3451 
   Size:        794      20    5121

  Fragments arranged by size: 

                5121     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5935
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564    2657    2692     529 
   Size:         35    2093      35    3772

  Fragments arranged by size: 

                3772    2093      35      35
 
DraI TTT'AAA

Cuts at:   3239    3239 
   Size:       5935
 
DraIII CAC_nnn'GTG

Cuts at:   2732    2732 
   Size:       5935
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4942     809 
   Size:        507    3626    1802

  Fragments arranged by size: 

                3626    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2503    2534    2748 
   Size:         54    1425      50     275      18     673      31     214

Cuts at:   2748    2964    3382    4271    5900       8 
   Size:        216     418     889    1629      43

  Fragments arranged by size: 

                1629    1425     889     673     418     275     216     214
                  54      50      43      31      18
 
EagI C'GGCC_G

Cuts at:   1537    1812    3382    1537 
   Size:        275    1570    4090

  Fragments arranged by size: 

                4090    1570     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2484    3865    1353 
   Size:       1131    1381    3423

  Fragments arranged by size: 

                3423    1381    1131
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    4522    4896    5042     387 
   Size:       1064     138    2933     374     146    1280

  Fragments arranged by size: 

                2933    1280    1064     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2509    2769    3051    3998    4595    5382    5650 
   Size:        393     877     260     282     947     597     787     268

Cuts at:   5650    1239 
   Size:       1524

  Fragments arranged by size: 

                1524     947     877     787     597     393     282     268
                 260
 
EcoO109I rG'GnC_Cy

Cuts at:   2275    2275 
   Size:       5935
 
EcoRI G'AATT_C

Cuts at:   3244    3622    3244 
   Size:        378    5557
 
FspI TGC'GCA

Cuts at:   4441    4441 
   Size:       5935
 
HaeII r_GCGC'y

Cuts at:   1678    5082    1678 
   Size:       3404    2531
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2577    2609    4576    4608    4650 
   Size:         32     135      32    1005      32    1967      32      42

Cuts at:   4650    4682    1373 
   Size:         32    2626

  Fragments arranged by size: 

                2626    1967    1005     135      42      32      32      32
                  32      32
 
HincII GTy'rAC

Cuts at:    669    1657    1938    3279    3525     669 
   Size:        988     281    1341     246    3079

  Fragments arranged by size: 

                3079    1341     988     281     246
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       5935
 
HpaI GTT'AAC

Cuts at:   3525    3525 
   Size:       5935
 
KpnI G_GTAC'C

Cuts at:   3260    3260 
   Size:       5935
 
MfeI C'AATT_G

Cuts at:   3534    3534 
   Size:       5935
 
MluI A'CGCG_T

Cuts at:   5811    5811 
   Size:       5935
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    2310    5049    5233    1305 
   Size:       1005    2739     184    2007

  Fragments arranged by size: 

                2739    2007    1005     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832    2536    2750    2966      10 
   Size:         54    1425     343     704     214     216    2979

  Fragments arranged by size: 

                2979    1425     704     343     216     214      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2396    3893    4252    4411     518 
   Size:        648    1230    1497     359     159    2042

  Fragments arranged by size: 

                2042    1497    1230     648     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    2163    2743    2789    3351    4028    5176    5421    1606 
   Size:        557     580      46     562     677    1148     245    2120

  Fragments arranged by size: 

                2120    1148     677     580     562     557     245      46
            
 
NcoI C'CATG_G

Cuts at:    513    1065    2004     513 
   Size:        552     939    4444

  Fragments arranged by size: 

                4444     939     552
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       5935
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5935
 
NotI GC'GGCC_GC

Cuts at:   3382    3382 
   Size:       5935
 
NspI r_CATG'y

Cuts at:   1132    1229    3293    4838    1132 
   Size:         97    2064    1545    2229

  Fragments arranged by size: 

                2229    2064    1545      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       5935
 
PciI A'CATG_T

Cuts at:   4834    4834 
   Size:       5935
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    2554    1108 
   Size:       1446    4489
 
PfoI T'CCnGG_A

Cuts at:   2256    2256 
   Size:       5935
 
PmeI GTTT'AAAC

Cuts at:   3239    3239 
   Size:       5935
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   4004    4036    5517    5549    4004 
   Size:         32    1481      32    4390

  Fragments arranged by size: 

                4390    1481      32      32
 
PpuMI rG'GwC_Cy

Cuts at:   2275    2275 
   Size:       5935
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5935
 
PsiI TTA'TAA

Cuts at:   3505    3505 
   Size:       5935
 
PspOMI G'GGCC_C

Cuts at:   3144    3144 
   Size:       5935
 
PstI C_TGCA'G

Cuts at:    830    3165    3287     830 
   Size:       2335     122    3478

  Fragments arranged by size: 

                3478    2335     122
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5935
 
PvuII CAG'CTG

Cuts at:   2163    2743    2789    2163 
   Size:        580      46    5309

  Fragments arranged by size: 

                5309     580      46
 
SalI G'TCGA_C

Cuts at:   1936    3277    1936 
   Size:       1341    4594
 
SbfI CC_TGCA'GG

Cuts at:   3165    3287    3165 
   Size:        122    5813
 
ScaI AGT'ACT

Cuts at:   4183    5895    4183 
   Size:       1712    4223
 
SfcI C'TryA_G

Cuts at:    826    1045    2335    3161    3283    4418    5099    5290     826 
   Size:        219    1290     826     122    1135     681     191    1471

  Fragments arranged by size: 

                1471    1290    1135     826     681     219     191     122
            
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5935
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5935
 
SmaI CCC'GGG

Cuts at:   1872    3262    1872 
   Size:       1390    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    2429    3005    4039    4940    5202     820 
   Size:        197     926     486     576    1034     901     262    1553

  Fragments arranged by size: 

                1553    1034     926     901     576     486     262     197
            
 
SnaBI TAC'GTA

Cuts at:    493    3726     493 
   Size:       3233    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5935
 
SphI G_CATG'C

Cuts at:   1132    3293    1132 
   Size:       2161    3774
 
SspI AAT'ATT

Cuts at:      5      52    3859       5 
   Size:         47    3807    2081

  Fragments arranged by size: 

                3807    2081      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       5935
 
StyI C'CwwG_G

Cuts at:    513    1065    2004    2751    3042    3374     513 
   Size:        552     939     747     291     332    3074

  Fragments arranged by size: 

                3074     939     747     552     332     291
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    2933    3962    3979    4132    4317    4762     325 
   Size:       1255    1353    1029      17     153     185     445    1498

  Fragments arranged by size: 

                1498    1353    1255    1029     445     185     153      17
            
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    2734    4181    5893 
   Size:        274      80      33      51    1443     757    1447    1712

Cuts at:   5893      96 
   Size:        138

  Fragments arranged by size: 

                1712    1447    1443     757     274     138      80      51
                  33
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2197    2229    2440    2472    2634    2666    5895    5927    2197 
   Size:         32     211      32     162      32    3229      32    2205

  Fragments arranged by size: 

                3229    2205     211     162      32      32      32      32
            
 
Tth111I GACn'n_nGTC

Cuts at:   1316    2180    2605    1316 
   Size:        864     425    4646

  Fragments arranged by size: 

                4646     864     425
 
XbaI T'CTAG_A

Cuts at:   3271    3271 
   Size:       5935
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5935
 
XmaI C'CCGG_G

Cuts at:   1870    3260    1870 
   Size:       1390    4545
 
XmnI GAAnn'nnTTC

Cuts at:   4064    4064 
   Size:       5935
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    3743     276 
   Size:         53      83     186     936    2209    2468

  Fragments arranged by size: 

                2468    2209     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI    AlwNI     ApaI    ApaLI     ApoI     AseI
    AsiSI     AvaI    BamHI     BanI    BanII     BbsI    BbvCI     BcgI
    BciVI     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI
     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI
    BstYI   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI
   DraIII     DrdI     EaeI     EagI     EarI     EciI  Eco57MI EcoO109I
    EcoRI     FspI    HaeII    Hin4I   HincII  HindIII     HpaI     KpnI
     MfeI     MluI     MmeI     MscI     MslI   MspA1I     NcoI     NdeI
     NheI     NotI     NspI     PasI     PciI    PflMI     PfoI     PmeI
     PpiI    PpuMI    PshAI     PsiI   PspOMI     PstI     PvuI    PvuII
     SalI     SbfI     ScaI     SfcI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SspI     StuI     StyI    TaqII     TatI
     TstI  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     AloI     AscI    AvrII     BaeI     BbeI    BfrBI
    BsiWI  BstZ17I  EcoICRI    EcoNI    EcoRV     FalI     FseI    FspAI
     KasI     NaeI     NarI   NgoMIV     NruI     NsiI     PacI     PmlI
    PspXI     PsrI    RsrII     SacI    SacII    SanDI     SapI    SexAI
     SfiI     SfoI     SrfI     SwaI     XcmI
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