Restriction map for pFN21AB0421
 (Linear) (Six-base) MAPSORT of: KIBB0421.seq  Check: 7897  from: 1  to: 1230



KIBB0421.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:13  ..

AccI GT'mk_AC

Cuts at:      0     977    1230 
   Size:        977     253
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     504    1046    1230 
   Size:        504     542     184

  Fragments arranged by size: 

                 542     504     184
 
AhdI GACnn_n'nnGTC

Cuts at:      0     586    1230 
   Size:        586     644
 
AlwNI CAG_nnn'CTG

Cuts at:      0     527    1066    1230 
   Size:        527     539     164

  Fragments arranged by size: 

                 539     527     164
 
ApaI G_GGCC'C

Cuts at:      0    1143    1230 
   Size:       1143      87
 
AvaI C'yCGr_G

Cuts at:      0     133     377    1230 
   Size:        133     244     853

  Fragments arranged by size: 

                 853     244     133
 
BamHI G'GATC_C

Cuts at:      0     137    1230 
   Size:        137    1093
 
BanII G_rGCy'C

Cuts at:      0     224     512    1143    1218    1230 
   Size:        224     288     631      75      12

  Fragments arranged by size: 

                 631     288     224      75      12
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     138     839    1034    1124    1230 
   Size:        138     701     195      90     106

  Fragments arranged by size: 

                 701     195     138     106      90
 
BbvCI CC'TCA_GC

Cuts at:      0    1103    1230 
   Size:       1103     127
 
BglI GCCn_nnn'nGGC

Cuts at:      0      30    1222    1230 
   Size:         30    1192       8

  Fragments arranged by size: 

                1192      30       8
 
Bme1580I G_kGCm'C

Cuts at:      0    1143    1230 
   Size:       1143      87
 
BmgBI CAC'GTC

Cuts at:      0     144    1230 
   Size:        144    1086
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     828     837    1230 
   Size:        828       9     393

  Fragments arranged by size: 

                 828     393       9
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      27    1230 
   Size:         27    1203
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     764    1230 
   Size:        764     466
 
Bpu10I CC'TnA_GC

Cuts at:      0    1103    1230 
   Size:       1103     127
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     409    1021    1230 
   Size:        409     612     209

  Fragments arranged by size: 

                 612     409     209
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     380     573     594     624     947    1230 
   Size:        380     193      21      30     323     283

  Fragments arranged by size: 

                 380     323     283     193      30      21
 
BsaWI w'CCGG_w

Cuts at:      0     244    1230 
   Size:        244     986
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     103     452     727    1230 
   Size:        103     349     275     503

  Fragments arranged by size: 

                 503     349     275     103
 
BseYI C'CCAG_C

Cuts at:      0     358    1081    1230 
   Size:        358     723     149

  Fragments arranged by size: 

                 723     358     149
 
BsiHKAI G_wGCw'C

Cuts at:      0     800    1230 
   Size:        800     430
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1203    1230 
   Size:       1203      27
 
Bsp1286I G_dGCh'C

Cuts at:      0     224     512     800    1143    1218    1230 
   Size:        224     288     288     343      75      12

  Fragments arranged by size: 

                 343     288     288     224      75      12
 
BspEI T'CCGG_A

Cuts at:      0     244    1230 
   Size:        244     986
 
BsrBI CCG'CTC

Cuts at:      0      30    1230 
   Size:         30    1200
 
BsrFI r'CCGG_y

Cuts at:      0      47    1230 
   Size:         47    1183
 
BsrGI T'GTAC_A

Cuts at:      0     729    1230 
   Size:        729     501
 
BssHII G'CGCG_C

Cuts at:      0     161    1230 
   Size:        161    1069
 
BssSI C'ACGA_G

Cuts at:      0      76     223     444    1230 
   Size:         76     147     221     786

  Fragments arranged by size: 

                 786     221     147      76
 
BstEII G'GTnAC_C

Cuts at:      0    1095    1230 
   Size:       1095     135
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     753    1230 
   Size:        753     477
 
BstYI r'GATC_y

Cuts at:      0     137    1230 
   Size:        137    1093
 
Bsu36I CC'TnA_GG

Cuts at:      0      12     479    1230 
   Size:         12     467     751

  Fragments arranged by size: 

                 751     467      12
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     673    1230 
   Size:        673     557
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     652     687    1230 
   Size:        652      35     543

  Fragments arranged by size: 

                 652     543      35
 
DraIII CAC_nnn'GTG

Cuts at:      0     727    1230 
   Size:        727     503
 
EaeI y'GGCC_r

Cuts at:      0     498     529     743     959    1230 
   Size:        498      31     214     216     271

  Fragments arranged by size: 

                 498     271     216     214      31
 
EarI CTCTTCn'nnn_

Cuts at:      0     479    1230 
   Size:        479     751
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     504     764    1046    1230 
   Size:        504     260     282     184

  Fragments arranged by size: 

                 504     282     260     184
 
EcoO109I rG'GnC_Cy

Cuts at:      0     270    1230 
   Size:        270     960
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     572     604    1230 
   Size:        572      32     626

  Fragments arranged by size: 

                 626     572      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     305    1230 
   Size:        305     925
 
MscI TGG'CCA

Cuts at:      0     531     745     961    1230 
   Size:        531     214     216     269

  Fragments arranged by size: 

                 531     269     216     214
 
MslI CAynn'nnrTG

Cuts at:      0     391    1230 
   Size:        391     839
 
MspA1I CmG'CkG

Cuts at:      0     158     738     784    1230 
   Size:        158     580      46     446

  Fragments arranged by size: 

                 580     446     158      46
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     549    1230 
   Size:        549     681
 
PfoI T'CCnGG_A

Cuts at:      0     251    1230 
   Size:        251     979
 
PpuMI rG'GwC_Cy

Cuts at:      0     270    1230 
   Size:        270     960
 
PspOMI G'GGCC_C

Cuts at:      0    1139    1230 
   Size:       1139      91
 
PstI C_TGCA'G

Cuts at:      0    1160    1230 
   Size:       1160      70
 
PvuII CAG'CTG

Cuts at:      0     158     738     784    1230 
   Size:        158     580      46     446

  Fragments arranged by size: 

                 580     446     158      46
 
SbfI CC_TGCA'GG

Cuts at:      0    1160    1230 
   Size:       1160      70
 
SfcI C'TryA_G

Cuts at:      0     330    1156    1230 
   Size:        330     826      74

  Fragments arranged by size: 

                 826     330      74
 
SmlI C'TyrA_G

Cuts at:      0     424    1000    1230 
   Size:        424     576     230

  Fragments arranged by size: 

                 576     424     230
 
StyI C'CwwG_G

Cuts at:      0     746    1037    1230 
   Size:        746     291     193

  Fragments arranged by size: 

                 746     291     193
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     928    1230 
   Size:        928     302
 
TatI w'GTAC_w

Cuts at:      0     729    1230 
   Size:        729     501
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     192     224     435     467     629     661    1230 
   Size:        192      32     211      32     162      32     569

  Fragments arranged by size: 

                 569     211     192     162      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     175     600    1230 
   Size:        175     425     630

  Fragments arranged by size: 

                 630     425     175
 

 Enzymes that do cut:

     AccI     AcuI     AhdI    AlwNI     ApaI     AvaI    BamHI    BanII
     BbsI    BbvCI     BglI Bme1580I    BmgBI     BmrI     BplI     BpmI
   Bpu10I    BpuEI     BsaI    BsaWI    BseRI    BseYI  BsiHKAI    BsmBI
 Bsp1286I    BspEI    BsrBI    BsrFI    BsrGI   BssHII    BssSI   BstEII
    BstXI    BstYI   Bsu36I    BtgZI    CspCI   DraIII     EaeI     EarI
  Eco57MI EcoO109I    Hin4I     MmeI     MscI     MslI   MspA1I    PflMI
     PfoI    PpuMI   PspOMI     PstI    PvuII     SbfI     SfcI     SmlI
     StyI    TaqII     TatI     TstI  Tth111I

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AleI     AlfI     AloI    ApaLI     ApoI     AscI     AseI    AsiSI
    AvrII     BaeI     BanI     BbeI     BcgI    BciVI     BclI    BfrBI
    BglII     BlpI     BmtI    BsaAI    BsaBI    BsaHI    BsaXI     BsgI
    BsiEI    BsiWI     BsmI    BspHI    BspMI    BsrDI   BstAPI    BstBI
  BstZ17I     BtgI     BtsI     ClaI     DraI     DrdI     EagI     EciI
  EcoICRI    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
    HaeII   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI     PmeI     PmlI     PpiI
    PshAI     PsiI    PspXI     PsrI     PvuI    RsrII     SacI    SacII
     SalI    SanDI     SapI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     SwaI     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI
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