Restriction map for
pFN21AB0415
(Linear) (Six-base) MAPSORT of: KIBB0415.seq Check: 5134 from: 1 to: 1404
KIBB0415.orf
With 182 enzymes: SgfI *
October 7, 2009 14:13 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1017 1064 1210 1404
Size: 1017 47 146 194
Fragments arranged by size:
1017 194 146 47
AgeI A'CCGG_T
Cuts at: 0 1290 1404
Size: 1290 114
AleI CACnn'nnGTG
Cuts at: 0 676 1264 1404
Size: 676 588 140
Fragments arranged by size:
676 588 140
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 893 1404
Size: 893 511
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 964 996 1404
Size: 964 32 408
Fragments arranged by size:
964 408 32
AlwNI CAG_nnn'CTG
Cuts at: 0 673 1404
Size: 673 731
ApaLI G'TGCA_C
Cuts at: 0 316 1404
Size: 316 1088
AvaI C'yCGr_G
Cuts at: 0 141 340 1326 1404
Size: 141 199 986 78
Fragments arranged by size:
986 199 141 78
BanII G_rGCy'C
Cuts at: 0 350 376 764 922 1188 1366 1404
Size: 350 26 388 158 266 178 38
Fragments arranged by size:
388 350 266 178 158 38 26
BbsI GAAGACnn'nnnn_
Cuts at: 0 164 482 1174 1404
Size: 164 318 692 230
Fragments arranged by size:
692 318 230 164
BbvCI CC'TCA_GC
Cuts at: 0 247 379 1404
Size: 247 132 1025
Fragments arranged by size:
1025 247 132
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 81 115 1404
Size: 81 34 1289
Fragments arranged by size:
1289 81 34
BfrBI ATG'CAT
Cuts at: 0 1212 1404
Size: 1212 192
BglI GCCn_nnn'nGGC
Cuts at: 0 37 1404
Size: 37 1367
Bme1580I G_kGCm'C
Cuts at: 0 320 882 937 1404
Size: 320 562 55 467
Fragments arranged by size:
562 467 320 55
BmgBI CAC'GTC
Cuts at: 0 1172 1404
Size: 1172 232
BmrI ACTGGGnnnn_n'
Cuts at: 0 186 1057 1258 1404
Size: 186 871 201 146
Fragments arranged by size:
871 201 186 146
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 802 867 947 1130 1404
Size: 802 65 80 183 274
Fragments arranged by size:
802 274 183 80 65
Bpu10I CC'TnA_GC
Cuts at: 0 184 247 379 1404
Size: 184 63 132 1025
Fragments arranged by size:
1025 184 132 63
BsaAI yAC'GTr
Cuts at: 0 864 1404
Size: 864 540
BsaHI Gr'CG_yC
Cuts at: 0 842 1404
Size: 842 562
BsaWI w'CCGG_w
Cuts at: 0 365 1290 1404
Size: 365 925 114
Fragments arranged by size:
925 365 114
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 110 140 709 739 1404
Size: 110 30 569 30 665
Fragments arranged by size:
665 569 110 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 358 901 1126 1404
Size: 358 543 225 278
Fragments arranged by size:
543 358 278 225
BseYI C'CCAG_C
Cuts at: 0 211 303 307 350 1300 1404
Size: 211 92 4 43 950 104
Fragments arranged by size:
950 211 104 92 43 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 277 889 1404
Size: 277 612 515
Fragments arranged by size:
612 515 277
BsiEI CG_ry'CG
Cuts at: 0 300 473 1404
Size: 300 173 931
Fragments arranged by size:
931 300 173
BsiHKAI G_wGCw'C
Cuts at: 0 185 320 415 443 764 1404
Size: 185 135 95 28 321 640
Fragments arranged by size:
640 321 185 135 95 28
Bsp1286I G_dGCh'C
Cuts at: 0 185 320 350 376 415 443 764 882
Size: 185 135 30 26 39 28 321 118
Cuts at: 882 922 937 1188 1366 1404
Size: 40 15 251 178 38
Fragments arranged by size:
321 251 185 178 135 118 40 39
38 30 28 26 15
BspEI T'CCGG_A
Cuts at: 0 365 1404
Size: 365 1039
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 145 1404
Size: 145 1259
BsrBI CCG'CTC
Cuts at: 0 52 851 1404
Size: 52 799 553
Fragments arranged by size:
799 553 52
BsrDI GCAATG_nn'
Cuts at: 0 390 1142 1404
Size: 390 752 262
Fragments arranged by size:
752 390 262
BsrFI r'CCGG_y
Cuts at: 0 192 295 1290 1404
Size: 192 103 995 114
Fragments arranged by size:
995 192 114 103
BssHII G'CGCG_C
Cuts at: 0 17 1404
Size: 17 1387
BstAPI GCAn_nnn'nTGC
Cuts at: 0 867 1404
Size: 867 537
BstXI CCAn_nnnn'nTGG
Cuts at: 0 271 1101 1308 1404
Size: 271 830 207 96
Fragments arranged by size:
830 271 207 96
Bsu36I CC'TnA_GG
Cuts at: 0 856 1404
Size: 856 548
BtgI C'CryG_G
Cuts at: 0 677 929 1357 1404
Size: 677 252 428 47
Fragments arranged by size:
677 428 252 47
BtsI GCAGTG_nn'
Cuts at: 0 671 1087 1404
Size: 671 416 317
Fragments arranged by size:
671 416 317
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 886 921 1404
Size: 886 35 483
Fragments arranged by size:
886 483 35
DraIII CAC_nnn'GTG
Cuts at: 0 180 867 1404
Size: 180 687 537
Fragments arranged by size:
687 537 180
EaeI y'GGCC_r
Cuts at: 0 5 38 297 470 680 1404
Size: 5 33 259 173 210 724
Fragments arranged by size:
724 259 210 173 33 5
EagI C'GGCC_G
Cuts at: 0 297 470 1404
Size: 297 173 934
Fragments arranged by size:
934 297 173
EarI CTCTTCn'nnn_
Cuts at: 0 364 1036 1404
Size: 364 672 368
Fragments arranged by size:
672 368 364
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 641 1205 1404
Size: 641 564 199
Fragments arranged by size:
641 564 199
EcoICRI GAG'CTC
Cuts at: 0 762 1404
Size: 762 642
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 802 867 947 1017 1064 1130 1210 1404
Size: 802 65 80 70 47 66 80 194
Fragments arranged by size:
802 194 80 80 70 66 65 47
EcoO109I rG'GnC_Cy
Cuts at: 0 403 813 1353 1404
Size: 403 410 540 51
Fragments arranged by size:
540 410 403 51
EcoRV GAT'ATC
Cuts at: 0 769 1404
Size: 769 635
FseI GG_CCGG'CC
Cuts at: 0 299 1404
Size: 299 1105
FspI TGC'GCA
Cuts at: 0 80 1404
Size: 80 1324
HaeII r_GCGC'y
Cuts at: 0 58 72 1404
Size: 58 14 1332
Fragments arranged by size:
1332 58 14
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 709 741 1309 1341 1404
Size: 709 32 568 32 63
Fragments arranged by size:
709 568 63 32 32
MscI TGG'CCA
Cuts at: 0 682 1404
Size: 682 722
MslI CAynn'nnrTG
Cuts at: 0 676 827 988 1264 1404
Size: 676 151 161 276 140
Fragments arranged by size:
676 276 161 151 140
MspA1I CmG'CkG
Cuts at: 0 307 695 967 1236 1404
Size: 307 388 272 269 168
Fragments arranged by size:
388 307 272 269 168
NaeI GCC'GGC
Cuts at: 0 194 297 1404
Size: 194 103 1107
Fragments arranged by size:
1107 194 103
NcoI C'CATG_G
Cuts at: 0 929 1404
Size: 929 475
NgoMIV G'CCGG_C
Cuts at: 0 192 295 1404
Size: 192 103 1109
Fragments arranged by size:
1109 192 103
NsiI A_TGCA'T
Cuts at: 0 1214 1404
Size: 1214 190
NspI r_CATG'y
Cuts at: 0 1212 1404
Size: 1212 192
PflMI CCAn_nnn'nTGG
Cuts at: 0 218 1404
Size: 218 1186
PfoI T'CCnGG_A
Cuts at: 0 482 539 727 952 1241 1404
Size: 482 57 188 225 289 163
Fragments arranged by size:
482 289 225 188 163 57
PmlI CAC'GTG
Cuts at: 0 864 1404
Size: 864 540
PpuMI rG'GwC_Cy
Cuts at: 0 403 813 1353 1404
Size: 403 410 540 51
Fragments arranged by size:
540 410 403 51
PspXI vC'TCGA_Gb
Cuts at: 0 1326 1404
Size: 1326 78
PstI C_TGCA'G
Cuts at: 0 201 1404
Size: 201 1203
PvuII CAG'CTG
Cuts at: 0 307 967 1236 1404
Size: 307 660 269 168
Fragments arranged by size:
660 307 269 168
SacI G_AGCT'C
Cuts at: 0 764 1404
Size: 764 640
SanDI GG'GwC_CC
Cuts at: 0 1353 1404
Size: 1353 51
SapI GCTCTTCn'nnn_
Cuts at: 0 364 1404
Size: 364 1040
SfcI C'TryA_G
Cuts at: 0 197 744 1404
Size: 197 547 660
Fragments arranged by size:
660 547 197
SgrAI Cr'CCGG_yG
Cuts at: 0 1290 1404
Size: 1290 114
SmaI CCC'GGG
Cuts at: 0 143 1404
Size: 143 1261
SmlI C'TyrA_G
Cuts at: 0 1326 1404
Size: 1326 78
SphI G_CATG'C
Cuts at: 0 1212 1404
Size: 1212 192
SspI AAT'ATT
Cuts at: 0 1026 1404
Size: 1026 378
StuI AGG'CCT
Cuts at: 0 1251 1404
Size: 1251 153
StyI C'CwwG_G
Cuts at: 0 64 929 940 1404
Size: 64 865 11 464
Fragments arranged by size:
865 464 64 11
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 168 1404
Size: 168 1236
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 110 142 1404
Size: 110 32 1262
Fragments arranged by size:
1262 110 32
XhoI C'TCGA_G
Cuts at: 0 1326 1404
Size: 1326 78
XmaI C'CCGG_G
Cuts at: 0 141 1404
Size: 141 1263
XmnI GAAnn'nnTTC
Cuts at: 0 279 1404
Size: 279 1125
Enzymes that do cut:
AcuI AgeI AleI AlfI AloI AlwNI ApaLI AvaI
BanII BbsI BbvCI BcgI BfrBI BglI Bme1580I BmgBI
BmrI BpmI Bpu10I BsaAI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspMI BsrBI
BsrDI BsrFI BssHII BstAPI BstXI Bsu36I BtgI BtsI
CspCI DraIII EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRV FseI FspI HaeII Hin4I MscI MslI
MspA1I NaeI NcoI NgoMIV NsiI NspI PflMI PfoI
PmlI PpuMI PspXI PstI PvuII SacI SanDI SapI
SfcI SgrAI SmaI SmlI SphI SspI StuI StyI
TaqII TstI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AhdI ApaI ApoI AscI AseI AsiSI AvrII BaeI
BamHI BanI BbeI BciVI BclI BglII BlpI BmtI
BplI BpuEI BsaI BsaBI BsiWI BsmI BsmBI BspHI
BsrGI BssSI BstBI BstEII BstYI BstZ17I BtgZI ClaI
DraI DrdI EcoNI EcoRI FalI FspAI HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI NarI NdeI
NheI NotI NruI PacI PasI PciI PmeI PpiI
PshAI PsiI PspOMI PsrI PvuI RsrII SacII SalI
SbfI ScaI SexAI SfiI SfoI SgfI SnaBI SpeI
SrfI SwaI TatI Tth111I XbaI XcmI ZraI