Restriction map for
pFN21AB0403
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# NO CUT ENZYMES ( 120 enzymes) #
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Acc65I AccIII AclI AcsI AflII
AflIII AgeI Alw44I AlwI AocI
ApaLI ApoI AscI AseI AsnI
Asp718I AspI AviII AvrII BamHI
BanI BbeI BbrPI BcgI BciVI
BfrI BlnI BsaAI BsaBI BsaWI
BseAI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstZ17I Bsu36I ClaI
CpoI DraI DrdI Eco81I EcoO65I
EcoRI EcoRV EcoT22I FseI FspI
HincII HindII HindIII HpaI KasI
KpnI MamI MfeI MluI MroI
MseI MunI NarI NdeI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SalI SapI
SbfI ScaI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
TfiI Tru9I Tsp509I Tth111I Van91I
XbaI XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AatII 241 GACGTC GACGT^C
AccI 244 GTCTAC GT^[AC][GT]AC
AhdI 1171 GACCTCACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 821 AGCGCT AGC^GCT
ApaI 1001 GGGCCC GGGCC^C
Asp700I 57 GAACCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1171 GACCTCACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 566 TGGCCA TGG^CCA
BanII 1001 GGGCCC G[GA]GC[CT]^C
BbsI 238 GAAGAC GAAGAC(2/6)
BclI 311 TGATCA T^GATCA
BglI 890 GCCGCTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 668 AGATCT A^GATCT
BlpI 125 GCTCAGC GC^T[ACGT]AGC
BpmI 538 CTGGAG CTGGAG(16/14)
Bpu1102I 125 GCTCAGC GC^T[ACGT]AGC
BpuAI 238 GAAGAC GAAGAC(2/6)
BsiEI 204 CGGCCG CG[GA][CT]^CG
BsrDI 1197 CATTGC GCAATG(2/0)
BstAPI 899 GCAGCGACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 668 AGATCT [GA]^GATC[CT]
CelII 125 GCTCAGC GC^T[ACGT]AGC
DraIII 744 CACTCCGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 204 CGGCCG C^GGCCG
Eam1105I 1171 GACCTCACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 180 GAAGAG CTCTTC(1/4)
EclXI 204 CGGCCG C^GGCCG
Eco47III 821 AGCGCT AGC^GCT
Eco52I 204 CGGCCG C^GGCCG
EcoNI 1236 CCTGGCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 311 TGATCA T^GATCA
HinfI 103 GACTC G^A[ACGT]TC
HphI 314 TCACC GGTGA(8/7)
Ksp632I 180 GAAGAG CTCTTC(1/4)
MflI 668 AGATCT [GA]^GATC[CT]
MluNI 566 TGGCCA TGG^CCA
MscI 566 TGGCCA TGG^CCA
NaeI 521 GCCGGC GCC^GGC
NcoI 1139 CCATGG C^CATGG
NgoMIV 521 GCCGGC G^CCGGC
NheI 805 GCTAGC G^CTAGC
PleI 103 GACTC GAGTC(4/5)
XhoII 668 AGATCT [GA]^GATC[CT]
XmnI 57 GAACCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 24 enzymes) #
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AlwNI 900 CAGCGACTG CAG[ACGT][ACGT][ACGT]^CTG
1063 CAGAGGCTG
AspHI 123 GTGCTC G[AT]GC[AT]^C
640 GAGCAC
AvaI 225 CTCGGG C^[CT]CG[GA]G
287 CCCGAG
BbvCI 1189 GCTGAGG CCTCAGC(-5/-2)
1240 GCTGAGG
BsaI 1385 GAGACC GGTCTC(1/5)
654 GGTCTC
BsiHKAI 123 GTGCTC G[AT]GC[AT]^C
640 GAGCAC
BsoBI 225 CTCGGG C^[CT]CG[GA]G
287 CCCGAG
BspMI 298 ACCTGC ACCTGC(4/8)
230 GCAGGT
BsrFI 521 GCCGGC [GA]^CCGG[CT]
1208 ACCGGC
Cfr10I 521 GCCGGC [GA]^CCGG[CT]
1208 ACCGGC
DraII 611 GGGACCT [GA]G^G[ACGT]CC[CT]
1232 AGGACCT
EaeI 204 CGGCCG [CT]^GGCC[GA]
566 TGGCCA
EcoO109I 611 GGGACCT [GA]G^G[ACGT]CC[CT]
1232 AGGACCT
EcoT14I 575 CCTTGG C^C[AT][AT]GG
1139 CCATGG
KspI 918 CCGCGG CCGC^GG
1253 CCGCGG
MaeIII 674 GTGAC ^GT[ACGT]AC
1052 GTGAC
MslI 1134 CACTCCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1197 CATTGCCATG
NspI 140 ACATGC [GA]CATG^[CT]
947 ACATGC
PpuMI 611 GGGACCT [GA]G^G[AT]CC[CT]
1232 AGGACCT
SacII 918 CCGCGG CCGC^GG
1253 CCGCGG
SexAI 467 ACCTGGT A^CC[AT]GGT
650 ACCAGGT
StuI 788 AGGCCT AGG^CCT
926 AGGCCT
StyI 575 CCTTGG C^C[AT][AT]GG
1139 CCATGG
Tsp45I 674 GTGAC ^GT[GC]AC
1052 GTGAC
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# 3 OR MORE CUT ENZYMES ( 70 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AvaII BbvI BcnI BfaI BmyI
BsaHI BsaJI BseRI BsgI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrI
BssKI BstF5I BstNI BstUI BstXI
Cac8I CfoI Cfr13I DdeI DpnII
DsaI EcoRII FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HpaII ItaI MaeI
MaeII MboI MboII MnlI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI TaqI TseI TspRI TthHB8I