(Circular) (Six-base) MAPSORT of: pFN21AB0403.seq Check: 7828 from: 1 to: 6100 pFN21AB0403 6100 bp With 182 enzymes: SgfI * April 15, 2010 09:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2250 3910 278 Size: 53 83 186 936 714 1660 2468 Fragments arranged by size: 2468 1660 936 714 186 83 53 AccI GT'mk_AC Cuts at: 1937 2250 3443 1937 Size: 313 1193 4594 Fragments arranged by size: 4594 1193 313 Acc65I G'GTAC_C Cuts at: 3421 3421 Size: 6100 AclI AA'CG_TT Cuts at: 1563 4227 4600 1563 Size: 2664 373 3063 Fragments arranged by size: 3063 2664 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2015 2782 4163 5547 5815 1632 Size: 383 767 1381 1384 268 1917 Fragments arranged by size: 1917 1384 1381 767 383 268 AfeI AGC'GCT Cuts at: 2828 2828 Size: 6100 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5903 AflIII A'CryG_T Cuts at: 1203 4999 5976 1203 Size: 3796 977 1327 Fragments arranged by size: 3796 1327 977 AgeI A'CCGG_T Cuts at: 4931 4931 Size: 6100 AhdI GACnn_n'nnGTC Cuts at: 3181 4829 3181 Size: 1648 4452 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6100 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6100 AlwNI CAG_nnn'CTG Cuts at: 2910 3073 3843 5415 2910 Size: 163 770 1572 3595 Fragments arranged by size: 3595 1572 770 163 ApaI G_GGCC'C Cuts at: 3010 3010 Size: 6100 ApaLI G'TGCA_C Cuts at: 4157 5313 4157 Size: 1156 4944 ApoI r'AATT_y Cuts at: 1493 3409 3635 3787 5896 1493 Size: 1916 226 152 2109 1697 Fragments arranged by size: 2109 1916 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4654 160 Size: 872 3622 1606 Fragments arranged by size: 3622 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6100 AvaI C'yCGr_G Cuts at: 1870 1943 2230 2292 3425 1870 Size: 73 287 62 1133 4545 Fragments arranged by size: 4545 1133 287 73 62 BamHI G'GATC_C Cuts at: 3430 3797 3430 Size: 367 5733 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3421 4876 618 Size: 325 217 627 1634 1455 1842 Fragments arranged by size: 1842 1634 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3010 3419 1391 Size: 1619 409 4072 Fragments arranged by size: 4072 1619 409 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2250 928 Size: 971 351 4778 Fragments arranged by size: 4778 971 351 BbvCI CC'TCA_GC Cuts at: 3195 3246 3195 Size: 51 6049 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4291 4325 1903 Size: 34 2354 34 3678 Fragments arranged by size: 3678 2354 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3992 5202 3992 Size: 1210 4890 BclI T'GATC_A Cuts at: 1548 2316 1548 Size: 768 5332 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2901 4711 136 Size: 107 122 71 631 605 1229 1810 1525 Fragments arranged by size: 1810 1525 1229 631 605 122 107 71 BglII A'GATC_T Cuts at: 2673 6095 2673 Size: 3422 2678 BlpI GC'TnA_GC Cuts at: 2131 3517 2131 Size: 1386 4714 Bme1580I G_kGCm'C Cuts at: 1790 3010 4161 5317 1790 Size: 1220 1151 1156 2573 Fragments arranged by size: 2573 1220 1156 1151 BmgBI CAC'GTC Cuts at: 1318 2569 3183 1318 Size: 1251 614 4235 Fragments arranged by size: 4235 1251 614 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3013 3319 3368 Size: 416 53 83 399 24 1596 306 49 Cuts at: 3368 3831 4789 442 Size: 463 958 1753 Fragments arranged by size: 1753 1596 958 463 416 399 306 83 53 49 24 BmtI G_CTAG'C Cuts at: 1056 2814 1056 Size: 1758 4342 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2564 4760 1239 Size: 1325 2196 2579 Fragments arranged by size: 2579 2196 1325 Bpu10I CC'TnA_GC Cuts at: 2507 3195 3246 2507 Size: 688 51 5361 Fragments arranged by size: 5361 688 51 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4225 5090 5388 4225 Size: 865 298 4937 Fragments arranged by size: 4937 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2665 3384 4763 882 Size: 617 1166 719 1379 2219 Fragments arranged by size: 2219 1379 1166 719 617 BsaAI yAC'GTr Cuts at: 493 1204 3891 493 Size: 711 2687 2702 Fragments arranged by size: 2702 2687 711 BsaBI GATnn'nnATC Cuts at: 6094 6094 Size: 6100 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2178 2247 2850 3060 Size: 53 83 186 936 645 69 603 210 Cuts at: 3060 3907 4289 4924 275 Size: 847 382 635 1451 Fragments arranged by size: 1451 936 847 645 635 603 382 210 186 83 69 53 BsaWI w'CCGG_w Cuts at: 4533 4931 5205 5352 5763 4533 Size: 398 274 147 411 4870 Fragments arranged by size: 4870 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2387 2417 2645 2675 2966 Size: 30 165 30 984 30 228 30 291 Cuts at: 2966 2996 3023 3053 1178 Size: 30 27 30 4225 Fragments arranged by size: 4225 984 291 228 165 30 30 30 30 30 30 27 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2468 2471 2819 3263 3266 1186 Size: 1282 3 348 444 3 4020 Fragments arranged by size: 4020 1282 444 348 3 3 BseYI C'CCAG_C Cuts at: 2272 2916 3225 5303 5799 2272 Size: 644 309 2078 496 2573 Fragments arranged by size: 2573 2078 644 496 309 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2030 2318 2582 1163 Size: 573 294 288 264 4681 Fragments arranged by size: 4681 573 294 288 264 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2212 3550 4311 5339 1540 Size: 275 186 211 1338 761 1028 2301 Fragments arranged by size: 2301 1338 1028 761 275 211 186 BsiHKAI G_wGCw'C Cuts at: 2132 2649 4161 4246 5317 2132 Size: 517 1512 85 1071 2915 Fragments arranged by size: 2915 1512 1071 517 85 BsmI GAATG_Cn' Cuts at: 3611 3704 3611 Size: 93 6007 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2132 2649 3010 3419 4161 4246 5317 Size: 399 342 517 361 409 742 85 1071 Cuts at: 5317 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 517 409 399 361 342 85 BspEI T'CCGG_A Cuts at: 5763 5763 Size: 6100 BspHI T'CATG_A Cuts at: 3989 5719 3989 Size: 1730 4370 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2226 2312 3455 844 Size: 1382 86 1143 3489 Fragments arranged by size: 3489 1382 1143 86 BsrBI CCG'CTC Cuts at: 1394 1823 3987 1394 Size: 429 2164 3507 Fragments arranged by size: 3507 2164 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3201 4595 4769 65 Size: 1183 1953 1394 174 1396 Fragments arranged by size: 1953 1396 1394 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2526 3213 4744 4931 1700 Size: 110 716 687 1531 187 2869 Fragments arranged by size: 2869 1531 716 687 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6100 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6100 BssSI C'ACGA_G Cuts at: 4160 5172 4160 Size: 1012 5088 BstAPI GCAn_nnn'nTGC Cuts at: 794 2910 794 Size: 2116 3984 BstBI TT'CG_AA Cuts at: 4904 4904 Size: 6100 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6100 BstXI CCAn_nnnn'nTGG Cuts at: 2557 2581 3049 3145 2557 Size: 24 468 96 5512 Fragments arranged by size: 5512 468 96 24 BstYI r'GATC_y Cuts at: 1972 2673 3430 3797 4182 4199 5651 5737 5749 Size: 701 757 367 385 17 1452 86 12 Cuts at: 5749 6095 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 757 701 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2101 2923 3144 3258 513 Size: 552 939 97 822 221 114 3355 Fragments arranged by size: 3355 939 822 552 221 114 97 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2590 487 Size: 683 56 1364 3997 Fragments arranged by size: 3997 1364 683 56 BtsI GCAGTG_nn' Cuts at: 2132 3616 4410 4430 2132 Size: 1484 794 20 3802 Fragments arranged by size: 3802 1484 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6100 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6065 DraI TTT'AAA Cuts at: 3404 3404 Size: 6100 DraIII CAC_nnn'GTG Cuts at: 2754 2754 Size: 6100 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5107 809 Size: 507 3791 1802 Fragments arranged by size: 3791 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2209 2571 3547 Size: 54 1425 50 275 18 379 362 976 Cuts at: 3547 4436 6065 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 976 889 379 362 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2209 3547 1537 Size: 275 397 1338 4090 Fragments arranged by size: 4090 1338 397 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2180 4030 1353 Size: 827 1850 3423 Fragments arranged by size: 3423 1850 827 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2780 4687 5061 5207 387 Size: 1064 138 1191 1907 374 146 1280 Fragments arranged by size: 1907 1280 1191 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2015 2564 2782 4163 4760 5547 5815 Size: 393 383 549 218 1381 597 787 268 Cuts at: 5815 1239 Size: 1524 Fragments arranged by size: 1524 1381 787 597 549 393 383 268 218 EcoNI CCTnn'n_nnAGG Cuts at: 3245 3245 Size: 6100 EcoO109I rG'GnC_Cy Cuts at: 2617 3238 2617 Size: 621 5479 EcoRI G'AATT_C Cuts at: 3409 3787 3409 Size: 378 5722 FspI TGC'GCA Cuts at: 4606 4606 Size: 6100 HaeII r_GCGC'y Cuts at: 1678 2286 2527 2580 2830 5247 1678 Size: 608 241 53 250 2417 2531 Fragments arranged by size: 2531 2417 608 250 241 53 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2213 2245 2487 2519 3023 Size: 32 135 32 641 32 242 32 504 Cuts at: 3023 3055 3167 3199 4741 4773 4815 4847 1373 Size: 32 112 32 1542 32 42 32 2626 Fragments arranged by size: 2626 1542 641 504 242 135 112 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3444 3690 669 Size: 988 281 1506 246 3079 Fragments arranged by size: 3079 1506 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6100 HpaI GTT'AAC Cuts at: 3690 3690 Size: 6100 KpnI G_GTAC'C Cuts at: 3425 3425 Size: 6100 MfeI C'AATT_G Cuts at: 3699 3699 Size: 6100 MluI A'CGCG_T Cuts at: 5976 5976 Size: 6100 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5214 5398 1305 Size: 3909 184 2007 Fragments arranged by size: 3909 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2573 10 Size: 54 1425 343 741 3537 Fragments arranged by size: 3537 1425 741 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3143 3206 4058 4417 4576 518 Size: 648 1977 63 852 359 159 2042 Fragments arranged by size: 2042 1977 852 648 359 159 63 MspA1I CmG'CkG Cuts at: 1606 2257 2697 2874 2898 2925 3165 3220 3260 Size: 651 440 177 24 27 240 55 40 Cuts at: 3260 3310 3343 3516 4193 5341 5586 1606 Size: 50 33 173 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 651 440 245 240 177 173 55 50 40 33 27 24 NaeI GCC'GGC Cuts at: 2528 2528 Size: 6100 NcoI C'CATG_G Cuts at: 513 1065 2004 3144 513 Size: 552 939 1140 3469 Fragments arranged by size: 3469 1140 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6100 NgoMIV G'CCGG_C Cuts at: 2526 2526 Size: 6100 NheI G'CTAG_C Cuts at: 1052 2810 1052 Size: 1758 4342 NotI GC'GGCC_GC Cuts at: 3547 3547 Size: 6100 NspI r_CATG'y Cuts at: 1132 1229 2149 2956 3458 5003 1132 Size: 97 920 807 502 1545 2229 Fragments arranged by size: 2229 1545 920 807 502 97 PasI CC'CwG_GG Cuts at: 1348 3102 1348 Size: 1754 4346 PciI A'CATG_T Cuts at: 4999 4999 Size: 6100 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6100 PfoI T'CCnGG_A Cuts at: 2172 2970 2172 Size: 798 5302 PmeI GTTT'AAAC Cuts at: 3404 3404 Size: 6100 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4169 4201 5682 5714 4169 Size: 32 1481 32 4555 Fragments arranged by size: 4555 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2617 3238 2617 Size: 621 5479 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6100 PsiI TTA'TAA Cuts at: 3670 3670 Size: 6100 PspOMI G'GGCC_C Cuts at: 3006 3006 Size: 6100 PstI C_TGCA'G Cuts at: 830 2127 2262 3096 3156 3309 3452 830 Size: 1297 135 834 60 153 143 3478 Fragments arranged by size: 3478 1297 834 153 143 135 60 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6100 PvuII CAG'CTG Cuts at: 2257 3165 3220 3310 2257 Size: 908 55 90 5047 Fragments arranged by size: 5047 908 90 55 SacII CC_GC'GG Cuts at: 2926 3261 2926 Size: 335 5765 SalI G'TCGA_C Cuts at: 1936 3442 1936 Size: 1506 4594 SbfI CC_TGCA'GG Cuts at: 3452 3452 Size: 6100 ScaI AGT'ACT Cuts at: 4348 6060 4348 Size: 1712 4388 SexAI A'CCwGG_T Cuts at: 2472 2655 2472 Size: 183 5917 SfcI C'TryA_G Cuts at: 826 1045 2123 2258 3092 3152 3305 3448 4583 Size: 219 1078 135 834 60 153 143 1135 Cuts at: 4583 5264 5455 826 Size: 681 191 1471 Fragments arranged by size: 1471 1135 1078 834 681 219 191 153 143 135 60 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6100 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6100 SmaI CCC'GGG Cuts at: 1872 3427 1872 Size: 1555 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4204 5105 5367 820 Size: 197 926 2261 901 262 1553 Fragments arranged by size: 2261 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3891 493 Size: 3398 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6100 SphI G_CATG'C Cuts at: 1132 3458 1132 Size: 2326 3774 SspI AAT'ATT Cuts at: 5 52 4024 5 Size: 47 3972 2081 Fragments arranged by size: 3972 2081 47 StuI AGG'CCT Cuts at: 1517 2795 2933 1517 Size: 1278 138 4684 Fragments arranged by size: 4684 1278 138 StyI C'CwwG_G Cuts at: 513 1065 2004 2580 3144 3539 513 Size: 552 939 576 564 395 3074 Fragments arranged by size: 3074 939 576 564 552 395 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4127 4144 4297 4482 4927 325 Size: 1255 2547 17 153 185 445 1498 Fragments arranged by size: 2547 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2769 4346 6058 Size: 274 80 33 51 1443 792 1577 1712 Cuts at: 6058 96 Size: 138 Fragments arranged by size: 1712 1577 1443 792 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3151 3183 6060 6092 3151 Size: 32 2877 32 3159 Fragments arranged by size: 3159 2877 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6100 XbaI T'CTAG_A Cuts at: 3436 3436 Size: 6100 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6100 XmaI C'CCGG_G Cuts at: 1870 3425 1870 Size: 1555 4545 XmnI GAAnn'nnTTC Cuts at: 2066 4229 2066 Size: 2163 3937 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2248 3908 276 Size: 53 83 186 936 714 1660 2468 Fragments arranged by size: 2468 1660 936 714 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SanDI SapI SfiI SfoI SrfI SwaI XcmI