Restriction map for
pFN21AB0403
(Linear) (Six-base) MAPSORT of: KIBB0403.seq Check: 3474 from: 1 to: 1395
KIBB0403.orf
With 182 enzymes: SgfI *
October 7, 2009 14:13 ..
AatII G_ACGT'C
Cuts at: 0 245 1395
Size: 245 1150
AccI GT'mk_AC
Cuts at: 0 245 1395
Size: 245 1150
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 10 777 1395
Size: 10 767 618
Fragments arranged by size:
767 618 10
AfeI AGC'GCT
Cuts at: 0 823 1395
Size: 823 572
AhdI GACnn_n'nnGTC
Cuts at: 0 1176 1395
Size: 1176 219
AlwNI CAG_nnn'CTG
Cuts at: 0 905 1068 1395
Size: 905 163 327
Fragments arranged by size:
905 327 163
ApaI G_GGCC'C
Cuts at: 0 1005 1395
Size: 1005 390
AvaI C'yCGr_G
Cuts at: 0 225 287 1395
Size: 225 62 1108
Fragments arranged by size:
1108 225 62
BanII G_rGCy'C
Cuts at: 0 1005 1395
Size: 1005 390
BbsI GAAGACnn'nnnn_
Cuts at: 0 245 1395
Size: 245 1150
BbvCI CC'TCA_GC
Cuts at: 0 1190 1241 1395
Size: 1190 51 154
Fragments arranged by size:
1190 154 51
BclI T'GATC_A
Cuts at: 0 311 1395
Size: 311 1084
BglI GCCn_nnn'nGGC
Cuts at: 0 896 1395
Size: 896 499
BglII A'GATC_T
Cuts at: 0 668 1395
Size: 668 727
BlpI GC'TnA_GC
Cuts at: 0 126 1395
Size: 126 1269
Bme1580I G_kGCm'C
Cuts at: 0 1005 1395
Size: 1005 390
BmgBI CAC'GTC
Cuts at: 0 564 1178 1395
Size: 564 614 217
Fragments arranged by size:
614 564 217
BmrI ACTGGGnnnn_n'
Cuts at: 0 1008 1314 1363 1395
Size: 1008 306 49 32
Fragments arranged by size:
1008 306 49 32
BmtI G_CTAG'C
Cuts at: 0 809 1395
Size: 809 586
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 559 1395
Size: 559 836
Bpu10I CC'TnA_GC
Cuts at: 0 502 1190 1241 1395
Size: 502 688 51 154
Fragments arranged by size:
688 502 154 51
BsaI GGTCTCn'nnnn_
Cuts at: 0 660 1379 1395
Size: 660 719 16
Fragments arranged by size:
719 660 16
BsaHI Gr'CG_yC
Cuts at: 0 173 242 845 1055 1395
Size: 173 69 603 210 340
Fragments arranged by size:
603 340 210 173 69
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 382 412 640 670 961 991 1018 1048
Size: 382 30 228 30 291 30 27 30
Cuts at: 1048 1395
Size: 347
Fragments arranged by size:
382 347 291 228 30 30 30 30
27
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 463 466 814 1258 1261 1395
Size: 463 3 348 444 3 134
Fragments arranged by size:
463 444 348 134 3 3
BseYI C'CCAG_C
Cuts at: 0 267 911 1220 1395
Size: 267 644 309 175
Fragments arranged by size:
644 309 267 175
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 25 313 577 1395
Size: 25 288 264 818
Fragments arranged by size:
818 288 264 25
BsiEI CG_ry'CG
Cuts at: 0 207 1395
Size: 207 1188
BsiHKAI G_wGCw'C
Cuts at: 0 127 644 1395
Size: 127 517 751
Fragments arranged by size:
751 517 127
Bsp1286I G_dGCh'C
Cuts at: 0 127 644 1005 1395
Size: 127 517 361 390
Fragments arranged by size:
517 390 361 127
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 221 307 1395
Size: 221 86 1088
Fragments arranged by size:
1088 221 86
BsrDI GCAATG_nn'
Cuts at: 0 1196 1395
Size: 1196 199
BsrFI r'CCGG_y
Cuts at: 0 521 1208 1395
Size: 521 687 187
Fragments arranged by size:
687 521 187
BstAPI GCAn_nnn'nTGC
Cuts at: 0 905 1395
Size: 905 490
BstXI CCAn_nnnn'nTGG
Cuts at: 0 552 576 1044 1140 1395
Size: 552 24 468 96 255
Fragments arranged by size:
552 468 255 96 24
BstYI r'GATC_y
Cuts at: 0 668 1395
Size: 668 727
BtgI C'CryG_G
Cuts at: 0 96 918 1139 1253 1395
Size: 96 822 221 114 142
Fragments arranged by size:
822 221 142 114 96
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 585 1395
Size: 585 810
BtsI GCAGTG_nn'
Cuts at: 0 127 1395
Size: 127 1268
DraIII CAC_nnn'GTG
Cuts at: 0 749 1395
Size: 749 646
EaeI y'GGCC_r
Cuts at: 0 204 566 1395
Size: 204 362 829
Fragments arranged by size:
829 362 204
EagI C'GGCC_G
Cuts at: 0 204 1395
Size: 204 1191
EarI CTCTTCn'nnn_
Cuts at: 0 175 1395
Size: 175 1220
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 775 1395
Size: 775 620
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 10 559 777 1395
Size: 10 549 218 618
Fragments arranged by size:
618 549 218 10
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1240 1395
Size: 1240 155
EcoO109I rG'GnC_Cy
Cuts at: 0 612 1233 1395
Size: 612 621 162
Fragments arranged by size:
621 612 162
HaeII r_GCGC'y
Cuts at: 0 281 522 575 825 1395
Size: 281 241 53 250 570
Fragments arranged by size:
570 281 250 241 53
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 208 240 482 514 1018 1050 1162 1194
Size: 208 32 242 32 504 32 112 32
Cuts at: 1194 1395
Size: 201
Fragments arranged by size:
504 242 208 201 112 32 32 32
32
MscI TGG'CCA
Cuts at: 0 568 1395
Size: 568 827
MslI CAynn'nnrTG
Cuts at: 0 1138 1201 1395
Size: 1138 63 194
Fragments arranged by size:
1138 194 63
MspA1I CmG'CkG
Cuts at: 0 252 692 869 893 920 1160 1215 1255
Size: 252 440 177 24 27 240 55 40
Cuts at: 1255 1305 1338 1395
Size: 50 33 57
Fragments arranged by size:
440 252 240 177 57 55 50 40
33 27 24
NaeI GCC'GGC
Cuts at: 0 523 1395
Size: 523 872
NcoI C'CATG_G
Cuts at: 0 1139 1395
Size: 1139 256
NgoMIV G'CCGG_C
Cuts at: 0 521 1395
Size: 521 874
NheI G'CTAG_C
Cuts at: 0 805 1395
Size: 805 590
NspI r_CATG'y
Cuts at: 0 144 951 1395
Size: 144 807 444
Fragments arranged by size:
807 444 144
PasI CC'CwG_GG
Cuts at: 0 1097 1395
Size: 1097 298
PfoI T'CCnGG_A
Cuts at: 0 167 965 1395
Size: 167 798 430
Fragments arranged by size:
798 430 167
PpuMI rG'GwC_Cy
Cuts at: 0 612 1233 1395
Size: 612 621 162
Fragments arranged by size:
621 612 162
PspOMI G'GGCC_C
Cuts at: 0 1001 1395
Size: 1001 394
PstI C_TGCA'G
Cuts at: 0 122 257 1091 1151 1304 1395
Size: 122 135 834 60 153 91
Fragments arranged by size:
834 153 135 122 91 60
PvuII CAG'CTG
Cuts at: 0 252 1160 1215 1305 1395
Size: 252 908 55 90 90
Fragments arranged by size:
908 252 90 90 55
SacII CC_GC'GG
Cuts at: 0 921 1256 1395
Size: 921 335 139
Fragments arranged by size:
921 335 139
SexAI A'CCwGG_T
Cuts at: 0 467 650 1395
Size: 467 183 745
Fragments arranged by size:
745 467 183
SfcI C'TryA_G
Cuts at: 0 118 253 1087 1147 1300 1395
Size: 118 135 834 60 153 95
Fragments arranged by size:
834 153 135 118 95 60
StuI AGG'CCT
Cuts at: 0 790 928 1395
Size: 790 138 467
Fragments arranged by size:
790 467 138
StyI C'CwwG_G
Cuts at: 0 575 1139 1395
Size: 575 564 256
Fragments arranged by size:
575 564 256
TatI w'GTAC_w
Cuts at: 0 764 1395
Size: 764 631
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1146 1178 1395
Size: 1146 32 217
Fragments arranged by size:
1146 217 32
XmnI GAAnn'nnTTC
Cuts at: 0 61 1395
Size: 61 1334
ZraI GAC'GTC
Cuts at: 0 243 1395
Size: 243 1152
Enzymes that do cut:
AatII AccI AcuI AfeI AhdI AlwNI ApaI AvaI
BanII BbsI BbvCI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BsaI BsaHI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrDI
BsrFI BstAPI BstXI BstYI BtgI BtgZI BtsI DraIII
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I HaeII
Hin4I MscI MslI MspA1I NaeI NcoI NgoMIV NheI
NspI PasI PfoI PpuMI PspOMI PstI PvuII SacII
SexAI SfcI StuI StyI TatI TstI XmnI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AflII AflIII AgeI AleI AlfI
AloI ApaLI ApoI AscI AseI AsiSI AvrII BaeI
BamHI BanI BbeI BcgI BciVI BfrBI BplI BpuEI
BsaAI BsaBI BsaWI BsiWI BsmI BsmBI BspEI BspHI
BsrBI BsrGI BssHII BssSI BstBI BstEII BstZ17I Bsu36I
ClaI CspCI DraI DrdI EcoICRI EcoRI EcoRV FalI
FseI FspI FspAI HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI NarI NdeI NotI NruI NsiI
PacI PciI PflMI PmeI PmlI PpiI PshAI PsiI
PspXI PsrI PvuI RsrII SacI SalI SanDI SapI
SbfI ScaI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI SwaI TaqII Tth111I
XbaI XcmI XhoI XmaI