Restriction map for
pFN21AB8545
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# NO CUT ENZYMES ( 110 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AflII AflIII AgeI AhdI Alw44I
AocI ApaI ApaLI AscI Asp700I
Asp718I AspEI AspI AviII AvrII
BamHI BbvCI BclI BcnI BfrI
BglI BlnI BsaBI BsaWI BseAI
BsiEI BsiWI BsmBI Bsp1407I BspDI
BspEI BspLU11I BspMI BsrBI BsrGI
BssHII BstBI BstUI Bsu36I ClaI
CpoI DraIII DrdI EagI Eam1105I
EclXI Eco52I Eco81I EcoRI EcoT22I
FauI FbaI FseI FspI HincII
HindII HindIII HpaI KpnI KspI
MamI MluI MroI MspA1I MvnI
NaeI NciI NdeI NgoMIV NotI
NruI NsiI NspV PacI PflFI
PflMI PinAI PmeI PshAI Psp1406I
PvuI PvuII RsrII SacII SalI
SbfI ScaI SexAI SfiI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SwaI Tth111I Van91I
XbaI XcmI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 53 enzymes) #
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Aor51HI 2764 AGCGCT AGC^GCT
AseI 1534 ATTAAT AT^TAAT
AsnI 1534 ATTAAT AT^TAAT
AspHI 1517 GAGCTC G[AT]GC[AT]^C
BalI 1212 TGGCCA TGG^CCA
BanII 1517 GAGCTC G[GA]GC[CT]^C
BbeI 2693 GGCGCC GGCGC^C
BbrPI 1283 CACGTG CAC^GTG
BcgI 2709 CGAGAAAGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 1933 AGATCT A^GATCT
BlpI 526 GCTCAGC GC^T[ACGT]AGC
BmyI 1517 GAGCTC G[AGT]GC[ACT]^C
Bpu1102I 526 GCTCAGC GC^T[ACGT]AGC
BsaAI 1283 CACGTG [CT]AC^GT[GA]
BsaI 1322 GGTCTC GGTCTC(1/5)
BsiHKAI 1517 GAGCTC G[AT]GC[AT]^C
BsmI 572 GAATGC GAATGC(1/-1)
Bsp1286I 1517 GAGCTC G[AGT]GC[ACT]^C
BspHI 1103 TCATGA T^CATGA
BsrDI 2937 CATTGC GCAATG(2/0)
BsrFI 2221 GCCGGT [GA]^CCGG[CT]
BssSI 1736 CACGAG CACGAG(-5/-1)
Bst1107I 1844 GTATAC GTA^TAC
BstAPI 697 GCAGAAGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1425 GGTTACC G^GT[ACGT]ACC
BstPI 1425 GGTTACC G^GT[ACGT]ACC
BstXI 1371 CCAACGGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 1844 GTATAC GTA^TAC
CelII 526 GCTCAGC GC^T[ACGT]AGC
Cfr10I 2221 GCCGGT [GA]^CCGG[CT]
DraI 810 TTTAAA TTT^AAA
DsaI 516 CCATGG C^C[GA][CT]GG
EaeI 1212 TGGCCA [CT]^GGCC[GA]
Eco47III 2764 AGCGCT AGC^GCT
EcoNI 1885 CCTCAGTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1425 GGTTACC G^GT[ACGT]ACC
HgaI 4 GCGTC GACGC(5/10)
KasI 2693 GGCGCC G^GCGCC
MfeI 430 CAATTG C^AATTG
MluNI 1212 TGGCCA TGG^CCA
MscI 1212 TGGCCA TGG^CCA
MunI 430 CAATTG C^AATTG
NarI 2693 GGCGCC GG^CGCC
NcoI 516 CCATGG C^CATGG
PmaCI 1283 CACGTG CAC^GTG
PmlI 1283 CACGTG CAC^GTG
PpuMI 2228 GGGACCT [GA]G^G[AT]CC[CT]
PshBI 1534 ATTAAT AT^TAAT
RcaI 1103 TCATGA T^CATGA
SacI 1517 GAGCTC GAGCT^C
SapI 1565 GAAGAGC GCTCTTC(1/4)
SfoI 2693 GGCGCC GGC^GCC
StuI 1893 AGGCCT AGG^CCT
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AciI 1130 GCGG CCGC(-3/-1)
1320 GCGG
AcyI 3 GGCGTC G[GA]^CG[CT]C
2693 GGCGCC
AlwNI 529 CAGCAACTG CAG[ACGT][ACGT][ACGT]^CTG
1808 CAGAGACTG
AvaI 2084 CTCGAG C^[CT]CG[GA]G
2879 CTCGAG
BanI 1594 GGCACC G^G[CT][GA]CC
2693 GGCGCC
BbsI 1454 GAAGAC GAAGAC(2/6)
62 GTCTTC
BciVI 2891 GGATAC GTATCC(6/5)
2625 GTATCC
BpuAI 1454 GAAGAC GAAGAC(2/6)
62 GTCTTC
BsaHI 3 GGCGTC G[GA]^CG[CT]C
2693 GGCGCC
BsgI 121 GTGCAG GTGCAG(16/14)
461 GTGCAG
BsoBI 2084 CTCGAG C^[CT]CG[GA]G
2879 CTCGAG
CfoI 2694 GCGC GCG^C
2765 GCGC
HaeII 2693 GGCGCC [GA]GCGC^[CT]
2764 AGCGCT
HapII 2222 CCGG C^CGG
2748 CCGG
HhaI 2694 GCGC GCG^C
2765 GCGC
Hin1I 3 GGCGTC G[GA]^CG[CT]C
2693 GGCGCC
HinP1I 2694 GCGC G^CGC
2765 GCGC
HpaII 2222 CCGG C^CGG
2748 CCGG
MaeII 921 ACGT A^CGT
1284 ACGT
MslI 567 CATACGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2248 CATACTGATG
MspI 2222 CCGG C^CGG
2748 CCGG
NheI 1672 GCTAGC G^CTAGC
2704 GCTAGC
PaeR7I 2084 CTCGAG C^TCGAG
2879 CTCGAG
PstI 43 CTGCAG CTGCA^G
298 CTGCAG
SspI 1531 AATATT AAT^ATT
1537 AATATT
Tsp45I 214 GTCAC ^GT[GC]AC
1281 GTCAC
XhoI 2084 CTCGAG C^TCGAG
2879 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 68 enzymes) #
#########################################
AccI AcsI AfaI AluI AlwI
ApoI AvaII BbvI BfaI BpmI
BsaJI BseRI BsiYI BslI BsmAI
BsmFI BsrI BssKI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
DraII EarI EcoO109I EcoRII EcoRV
EcoT14I Fnu4HI FokI HaeIII HinfI
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MflI MnlI MseI
MvaI MwoI NdeII NlaIII NlaIV
NspI PleI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI TfiI Tru9I TseI Tsp509I
TspRI TthHB8I XhoII