Restriction map for
pFN21AB6115
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# NO CUT ENZYMES ( 117 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I Aor51HI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII AvrII BbeI BbrPI
BciVI BfaI BfrI BglI BlnI
BlpI Bpu1102I BsaWI BseAI BsiEI
BsiWI BsmBI BsmI Bsp1407I BspEI
BspHI BspLU11I BsrBI BsrGI BssHII
BssSI Bst1107I BstBI BstZ17I CelII
CpoI DraI EagI Eam1105I EclXI
Eco47III Eco52I EcoRI EcoRV EcoT22I
FseI FspI HindIII HpaI KasI
KpnI MaeI MfeI MluI MroI
MseI MunI NarI NdeI NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
RcaI RsrII SalI SbfI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI TfiI Tru9I
Tsp509I Tth111I XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 36 enzymes) #
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AcyI 921 GGCGTC G[GA]^CG[CT]C
AocI 782 CCTCAGG CC^T[ACGT]AGG
ApaI 72 GGGCCC GGGCC^C
BamHI 530 GGATCC G^GATCC
BbvCI 1211 CCTCAGC CCTCAGC(-5/-2)
BcgI 191 GCAAGAAGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1289 TGATCA T^GATCA
BglII 398 AGATCT A^GATCT
BsaAI 820 TACGTG [CT]AC^GT[GA]
BsaHI 921 GGCGTC G[GA]^CG[CT]C
BsaI 181 GAGACC GGTCTC(1/5)
BspDI 1303 ATCGAT AT^CGAT
BstAPI 1122 GCACAGTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 852 GGTCACC G^GT[ACGT]ACC
BstPI 852 GGTCACC G^GT[ACGT]ACC
Bsu36I 782 CCTCAGG CC^T[ACGT]AGG
ClaI 1303 ATCGAT AT^CGAT
DraIII 1123 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1033 GACGCGATTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 782 CCTCAGG CC^T[ACGT]AGG
EcoO65I 852 GGTCACC G^GT[ACGT]ACC
FauI 147 CCCGC CCCGC(4/6)
FbaI 1289 TGATCA T^GATCA
Hin1I 921 GGCGTC G[GA]^CG[CT]C
HincII 898 GTTGAC GT[CT]^[GA]AC
HindII 898 GTTGAC GT[CT]^[GA]AC
NaeI 1438 GCCGGC GCC^GGC
NgoMIV 1438 GCCGGC G^CCGGC
PflMI 242 CCAACGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1753 GGGTCCT [GA]G^G[AT]CC[CT]
PshAI 26 GACTCATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 1740 CTGCAG CTGCA^G
PvuII 1315 CAGCTG CAG^CTG
ScaI 644 AGTACT AGT^ACT
SmlI 1852 CTCAAG C^T[CT][GA]AG
Van91I 242 CCAACGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 21 enzymes) #
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AvaI 603 CTCGGG C^[CT]CG[GA]G
1789 CCCGAG
BanI 286 GGTGCC G^G[CT][GA]CC
1713 GGTGCC
BsaBI 571 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1167 GATCTTCATC
BseRI 504 CTCCTC GAGGAG(10/8)
1074 CTCCTC
BsmAI 181 GAGAC GTCTC(1/5)
596 GAGAC
BsoBI 603 CTCGGG C^[CT]CG[GA]G
1789 CCCGAG
BspMI 305 ACCTGC ACCTGC(4/8)
633 ACCTGC
BsrDI 321 CATTGC GCAATG(2/0)
1782 CATTGC
BsrFI 908 ACCGGC [GA]^CCGG[CT]
1438 GCCGGC
BstYI 398 AGATCT [GA]^GATC[CT]
530 GGATCC
Cfr10I 908 ACCGGC [GA]^CCGG[CT]
1438 GCCGGC
DraII 699 GGGGCCT [GA]G^G[ACGT]CC[CT]
1753 GGGTCCT
EcoNI 96 CCTTGTCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
762 CCTCTGGAAGG
EcoO109I 699 GGGGCCT [GA]G^G[ACGT]CC[CT]
1753 GGGTCCT
HaeII 486 GGCGCT [GA]GCGC^[CT]
1264 AGCGCC
KspI 148 CCGCGG CCGC^GG
706 CCGCGG
MamI 571 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1167 GATCTTCATC
MflI 398 AGATCT [GA]^GATC[CT]
530 GGATCC
SacII 148 CCGCGG CCGC^GG
706 CCGCGG
SfcI 1026 CTACAG C^T[GA][CT]AG
1740 CTGCAG
XhoII 398 AGATCT [GA]^GATC[CT]
530 GGATCC
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# 3 OR MORE CUT ENZYMES ( 84 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AspHI AvaII BalI BanII
BbsI BbvI BcnI BmyI BpmI
BpuAI BsaJI BsgI BsiHKAI BsiYI
BslI BsmFI Bsp1286I BsrI BssKI
BstF5I BstNI BstUI BstXI Cac8I
CfoI Cfr13I DdeI DpnII DsaI
EaeI EarI EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeII MaeIII MboI MboII
MluNI MnlI MscI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NcoI NdeII NlaIII NlaIV PleI
RsaI SacI SapI Sau3AI Sau96I
ScrFI SfaNI StyI TaqI TseI
Tsp45I TspRI TthHB8I XcmI