(Circular) (Six-base) MAPSORT of: pFN21AB6115.seq Check: 8490 from: 1 to: 6565 pFN21AB6115 6565 bp With 182 enzymes: SgfI * April 15, 2010 09:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2647 2647 Size: 6565 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4375 278 Size: 53 83 186 936 2839 2468 Fragments arranged by size: 2839 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3908 1937 Size: 1971 4594 Acc65I G'GTAC_C Cuts at: 3886 3886 Size: 6565 AclI AA'CG_TT Cuts at: 1563 4692 5065 1563 Size: 3129 373 3063 Fragments arranged by size: 3129 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2746 3616 4628 6012 6280 1632 Size: 1114 870 1012 1384 268 1917 Fragments arranged by size: 1917 1384 1114 1012 870 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6368 AflIII A'CryG_T Cuts at: 1203 5464 6441 1203 Size: 4261 977 1327 Fragments arranged by size: 4261 1327 977 AgeI A'CCGG_T Cuts at: 5396 5396 Size: 6565 AhdI GACnn_n'nnGTC Cuts at: 5294 5294 Size: 6565 AleI CACnn'nnGTG Cuts at: 1166 2954 1166 Size: 1788 4777 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6565 AlwNI CAG_nnn'CTG Cuts at: 2769 3325 3591 4308 5880 2769 Size: 556 266 717 1572 3454 Fragments arranged by size: 3454 1572 717 556 266 ApaI G_GGCC'C Cuts at: 2081 2081 Size: 6565 ApaLI G'TGCA_C Cuts at: 4622 5778 4622 Size: 1156 5409 ApoI r'AATT_y Cuts at: 1493 3874 4100 4252 6361 1493 Size: 2381 226 152 2109 1697 Fragments arranged by size: 2381 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5119 160 Size: 872 4087 1606 Fragments arranged by size: 4087 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6565 AvaI C'yCGr_G Cuts at: 1870 1943 2608 3794 3890 1870 Size: 73 665 1186 96 4545 Fragments arranged by size: 4545 1186 665 96 73 BamHI G'GATC_C Cuts at: 2535 3895 4262 2535 Size: 1360 367 4838 Fragments arranged by size: 4838 1360 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2291 3718 3886 5341 618 Size: 325 217 627 504 1427 168 1455 1842 Fragments arranged by size: 1842 1455 1427 627 504 325 217 168 BanII G_rGCy'C Cuts at: 1391 2067 2081 2097 2165 2529 3306 3345 3884 Size: 676 14 16 68 364 777 39 539 Cuts at: 3884 1391 Size: 4072 Fragments arranged by size: 4072 777 676 539 364 68 39 16 14 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2031 2789 3088 3226 3409 928 Size: 971 132 758 299 138 183 4084 Fragments arranged by size: 4084 971 758 299 183 138 132 BbvCI CC'TCA_GC Cuts at: 3217 3217 Size: 6565 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2185 2219 4756 4790 1903 Size: 34 248 34 2537 34 3678 Fragments arranged by size: 3678 2537 248 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4457 5667 4457 Size: 1210 5355 BclI T'GATC_A Cuts at: 1548 3294 1548 Size: 1746 4819 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5176 136 Size: 107 122 71 631 605 3504 1525 Fragments arranged by size: 3504 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2403 6560 2403 Size: 4157 2408 BlpI GC'TnA_GC Cuts at: 3982 3982 Size: 6565 Bme1580I G_kGCm'C Cuts at: 1790 2081 3138 3723 4626 5782 1790 Size: 291 1057 585 903 1156 2573 Fragments arranged by size: 2573 1156 1057 903 585 291 BmgBI CAC'GTC Cuts at: 1318 3148 3450 1318 Size: 1830 302 4433 Fragments arranged by size: 4433 1830 302 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3679 4296 5254 Size: 416 53 83 399 24 2262 617 958 Cuts at: 5254 442 Size: 1753 Fragments arranged by size: 2262 1753 958 617 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6565 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2144 2144 Size: 6565 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2578 2889 2986 5225 1239 Size: 1339 311 97 2239 2579 Fragments arranged by size: 2579 2239 1339 311 97 Bpu10I CC'TnA_GC Cuts at: 2058 2897 3217 2058 Size: 839 320 5406 Fragments arranged by size: 5406 839 320 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3842 4690 5555 5853 3842 Size: 848 865 298 4554 Fragments arranged by size: 4554 865 848 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2180 5228 882 Size: 617 681 3048 2219 Fragments arranged by size: 3048 2219 681 617 BsaAI yAC'GTr Cuts at: 493 1204 2827 4356 493 Size: 711 1623 1529 2702 Fragments arranged by size: 2702 1623 1529 711 BsaBI GATnn'nnATC Cuts at: 2580 3176 6559 2580 Size: 596 3383 2586 Fragments arranged by size: 3383 2586 596 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2927 4372 4754 5389 Size: 53 83 186 936 1394 1445 382 635 Cuts at: 5389 275 Size: 1451 Fragments arranged by size: 1451 1445 1394 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4998 5396 5670 5817 6228 4998 Size: 398 274 147 411 5335 Fragments arranged by size: 5335 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6340 Fragments arranged by size: 6340 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2500 3070 1186 Size: 1314 570 4681 Fragments arranged by size: 4681 1314 570 BseYI C'CCAG_C Cuts at: 2330 3551 3682 3687 5768 6264 2330 Size: 1221 131 5 2081 496 2631 Fragments arranged by size: 2631 2081 1221 496 131 5 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2144 2480 2654 3311 1163 Size: 573 408 336 174 657 4417 Fragments arranged by size: 4417 657 573 408 336 174 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4015 4776 5804 1540 Size: 275 186 2014 761 1028 2301 Fragments arranged by size: 2301 2014 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2097 2529 3345 3471 4626 4711 5782 2097 Size: 432 816 126 1155 85 1071 2880 Fragments arranged by size: 2880 1155 1071 816 432 126 85 BsmI GAATG_Cn' Cuts at: 4076 4169 4076 Size: 93 6472 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2067 2081 2097 2165 2529 3138 3306 Size: 399 277 14 16 68 364 609 168 Cuts at: 3306 3345 3471 3723 3884 4626 4711 5782 1391 Size: 39 126 252 161 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 609 399 364 277 252 168 161 126 85 68 39 16 14 BspEI T'CCGG_A Cuts at: 6228 6228 Size: 6565 BspHI T'CATG_A Cuts at: 4454 6184 4454 Size: 1730 4835 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2319 2647 3920 844 Size: 1475 328 1273 3489 Fragments arranged by size: 3489 1475 1273 328 BsrBI CCG'CTC Cuts at: 1394 1823 4452 1394 Size: 429 2629 3507 Fragments arranged by size: 3507 2629 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2325 3786 5060 5234 65 Size: 1183 1077 1461 1274 174 1396 Fragments arranged by size: 1461 1396 1274 1183 1077 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2913 3443 5209 5396 1700 Size: 110 1103 530 1766 187 2869 Fragments arranged by size: 2869 1766 1103 530 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6565 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6565 BssSI C'ACGA_G Cuts at: 4625 5637 4625 Size: 1012 5553 BstAPI GCAn_nnn'nTGC Cuts at: 794 3133 794 Size: 2339 4226 BstBI TT'CG_AA Cuts at: 5369 5369 Size: 6565 BstEII G'GTnAC_C Cuts at: 1184 2857 1184 Size: 1673 4892 BstXI CCAn_nnnn'nTGG Cuts at: 2074 2687 3487 3682 3715 2074 Size: 613 800 195 33 4924 Fragments arranged by size: 4924 800 613 195 33 BstYI r'GATC_y Cuts at: 1972 2403 2535 3895 4262 4647 4664 6116 6202 Size: 431 132 1360 367 385 17 1452 86 Cuts at: 6202 6214 6560 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1360 431 385 367 346 132 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2788 2788 Size: 6565 BtgI C'CryG_G Cuts at: 513 1065 2025 2043 2073 2153 2166 2520 2686 Size: 552 960 18 30 80 13 354 166 Cuts at: 2686 2711 3228 3273 3708 3714 513 Size: 25 517 45 435 6 3364 Fragments arranged by size: 3364 960 552 517 435 354 166 80 45 30 25 18 13 6 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2467 3181 3679 487 Size: 683 56 1241 714 498 3373 Fragments arranged by size: 3373 1241 714 683 498 56 BtsI GCAGTG_nn' Cuts at: 2539 2835 4081 4875 4895 2539 Size: 296 1246 794 20 4209 Fragments arranged by size: 4209 1246 794 296 20 ClaI AT'CG_AT Cuts at: 1554 3309 1554 Size: 1755 4810 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2266 2301 529 Size: 35 1702 35 4793 Fragments arranged by size: 4793 1702 35 35 DraI TTT'AAA Cuts at: 3869 3869 Size: 6565 DraIII CAC_nnn'GTG Cuts at: 3133 3133 Size: 6565 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3044 5572 809 Size: 507 1728 2528 1802 Fragments arranged by size: 2528 1802 1728 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2244 2365 2946 Size: 54 1425 50 275 18 414 121 581 Cuts at: 2946 3150 3711 4012 4901 6530 8 Size: 204 561 301 889 1629 43 Fragments arranged by size: 1629 1425 889 581 561 414 301 275 204 121 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4012 1537 Size: 275 2200 4090 Fragments arranged by size: 4090 2200 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2321 2510 3350 3359 4495 1353 Size: 968 189 840 9 1136 3423 Fragments arranged by size: 3423 1136 968 840 189 9 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3836 5152 5526 5672 387 Size: 1064 138 2247 1316 374 146 1280 Fragments arranged by size: 2247 1316 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2095 2527 3343 2095 Size: 432 816 5317 Fragments arranged by size: 5317 816 432 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2578 2746 2889 2986 3616 4628 5225 Size: 393 946 168 143 97 630 1012 597 Cuts at: 5225 6012 6280 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1012 946 787 630 597 393 268 168 143 97 EcoNI CCTnn'n_nnAGG Cuts at: 2105 2771 2105 Size: 666 5899 EcoO109I rG'GnC_Cy Cuts at: 2705 3759 2705 Size: 1054 5511 EcoRI G'AATT_C Cuts at: 3874 4252 3874 Size: 378 6187 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2224 2224 Size: 6565 FspI TGC'GCA Cuts at: 5071 5071 Size: 6565 HaeII r_GCGC'y Cuts at: 1678 2495 3273 5712 1678 Size: 817 778 2439 2531 Fragments arranged by size: 2531 2439 817 778 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3456 3488 3642 3674 5206 Size: 32 135 32 1884 32 154 32 1532 Cuts at: 5206 5238 5280 5312 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1884 1532 154 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2905 3909 4155 669 Size: 988 281 967 1004 246 3079 Fragments arranged by size: 3079 1004 988 967 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6565 HpaI GTT'AAC Cuts at: 4155 4155 Size: 6565 KpnI G_GTAC'C Cuts at: 3890 3890 Size: 6565 MfeI C'AATT_G Cuts at: 4164 4164 Size: 6565 MluI A'CGCG_T Cuts at: 6441 6441 Size: 6565 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5679 5863 1305 Size: 4374 184 2007 Fragments arranged by size: 4374 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2246 3152 3713 10 Size: 54 1425 343 414 906 561 2862 Fragments arranged by size: 2862 1425 906 561 414 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2586 2954 3485 3713 4523 4882 5041 Size: 648 1420 368 531 228 810 359 159 Cuts at: 5041 518 Size: 2042 Fragments arranged by size: 2042 1420 810 648 531 368 359 228 159 MspA1I CmG'CkG Cuts at: 1606 2155 2390 2713 3322 3547 3981 4658 5806 Size: 549 235 323 609 225 434 677 1148 Cuts at: 5806 6051 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 609 549 434 323 245 235 225 NaeI GCC'GGC Cuts at: 3445 3445 Size: 6565 NcoI C'CATG_G Cuts at: 513 1065 2686 3273 3708 3714 513 Size: 552 1621 587 435 6 3364 Fragments arranged by size: 3364 1621 587 552 435 6 NdeI CA'TA_TG Cuts at: 387 387 Size: 6565 NgoMIV G'CCGG_C Cuts at: 3443 3443 Size: 6565 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6565 NotI GC'GGCC_GC Cuts at: 4012 4012 Size: 6565 NspI r_CATG'y Cuts at: 1132 1229 3923 5468 1132 Size: 97 2694 1545 2229 Fragments arranged by size: 2694 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2968 3256 1348 Size: 1620 288 4657 Fragments arranged by size: 4657 1620 288 PciI A'CATG_T Cuts at: 5464 5464 Size: 6565 PflMI CCAn_nnn'nTGG Cuts at: 1108 2253 1108 Size: 1145 5420 PfoI T'CCnGG_A Cuts at: 2552 3426 2552 Size: 874 5691 PmeI GTTT'AAAC Cuts at: 3869 3869 Size: 6565 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4634 4666 6147 6179 4634 Size: 32 1481 32 5020 Fragments arranged by size: 5020 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3759 3759 Size: 6565 PshAI GACnn'nnGTC Cuts at: 1613 2035 1613 Size: 422 6143 PsiI TTA'TAA Cuts at: 4135 4135 Size: 6565 PspOMI G'GGCC_C Cuts at: 2077 2077 Size: 6565 PstI C_TGCA'G Cuts at: 830 3749 3917 830 Size: 2919 168 3478 Fragments arranged by size: 3478 2919 168 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6565 PvuII CAG'CTG Cuts at: 3322 3322 Size: 6565 SacI G_AGCT'C Cuts at: 2097 2529 3345 2097 Size: 432 816 5317 Fragments arranged by size: 5317 816 432 SacII CC_GC'GG Cuts at: 2156 2714 2156 Size: 558 6007 SalI G'TCGA_C Cuts at: 1936 3907 1936 Size: 1971 4594 SapI GCTCTTCn'nnn_ Cuts at: 2321 3350 3359 2321 Size: 1029 9 5527 Fragments arranged by size: 5527 1029 9 SbfI CC_TGCA'GG Cuts at: 3917 3917 Size: 6565 ScaI AGT'ACT Cuts at: 2651 4813 6525 2651 Size: 2162 1712 2691 Fragments arranged by size: 2691 2162 1712 SfcI C'TryA_G Cuts at: 826 1045 3031 3745 3913 5048 5729 5920 826 Size: 219 1986 714 168 1135 681 191 1471 Fragments arranged by size: 1986 1471 1135 714 681 219 191 168 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6565 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6565 SmaI CCC'GGG Cuts at: 1872 3892 1872 Size: 2020 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3857 4669 5570 5832 820 Size: 197 926 1914 812 901 262 1553 Fragments arranged by size: 1914 1553 926 901 812 262 197 SnaBI TAC'GTA Cuts at: 493 4356 493 Size: 3863 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6565 SphI G_CATG'C Cuts at: 1132 3923 1132 Size: 2791 3774 SspI AAT'ATT Cuts at: 5 52 4489 5 Size: 47 4437 2081 Fragments arranged by size: 4437 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6565 StyI C'CwwG_G Cuts at: 513 1065 2686 3153 3273 3708 3714 4004 513 Size: 552 1621 467 120 435 6 290 3074 Fragments arranged by size: 3074 1621 552 467 435 290 120 6 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2966 3266 4592 4609 4762 4947 5392 Size: 1255 1386 300 1326 17 153 185 445 Cuts at: 5392 325 Size: 1498 Fragments arranged by size: 1498 1386 1326 1255 445 300 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2649 3558 4811 Size: 274 80 33 51 1443 672 909 1253 Cuts at: 4811 6523 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1253 909 672 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6525 6557 6525 Size: 32 6533 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6565 XbaI T'CTAG_A Cuts at: 3901 3901 Size: 6565 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2684 2824 3145 3280 3706 2684 Size: 140 321 135 426 5543 Fragments arranged by size: 5543 426 321 140 135 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6565 XmaI C'CCGG_G Cuts at: 1870 3890 1870 Size: 2020 4545 XmnI GAAnn'nnTTC Cuts at: 4694 4694 Size: 6565 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4373 276 Size: 53 83 186 936 2839 2468 Fragments arranged by size: 2839 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstZ17I EcoRV FseI FspAI KasI NarI NruI NsiI PacI PmlI PspXI PsrI RsrII SanDI SexAI SfiI SfoI SrfI SwaI