Restriction map for
pFN21AB8496
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# NO CUT ENZYMES ( 104 enzymes) #
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AatII AccI AccII AccIII AclI
AcyI AflII AgeI AhdI AlwNI
Aor51HI ApaI AscI AspEI AviII
AvrII BamHI BbeI BbrPI BbvCI
BclI BfrI BglI BlnI BlpI
Bpu1102I BsaAI BsaHI BseAI BsiEI
BsiWI BsmBI BsmI BspDI BspEI
BsrDI BsrFI BssHII BssSI Bst1107I
BstUI BstZ17I CelII Cfr10I ClaI
CpoI DraI DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoRV EcoT22I
FauI FbaI FseI FspI HaeII
HgaI Hin1I HindIII HpaI KasI
KspI MluI MroI MvnI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI PacI PinAI PmaCI
PmeI PmlI PshAI Psp1406I PvuI
RsrII SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SwaI XbaI XmaCI XmaI
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# ONE CUT ENZYMES ( 42 enzymes) #
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Acc65I 429 GGTACC G^GTACC
Alw44I 323 GTGCAC G^TGCAC
ApaLI 323 GTGCAC G^TGCAC
AseI 370 ATTAAT AT^TAAT
AsnI 370 ATTAAT AT^TAAT
Asp718I 429 GGTACC G^GTACC
AspI 2009 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 623 TGGCCA TGG^CCA
BcgI 1176 GCAGCAGCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1133 GTATCC GTATCC(6/5)
BsaBI 132 GATTCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsrBI 1409 CCGCTC CCGCTC(-3/-3)
BstAPI 1743 GCAAATCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CfoI 2569 GCGC GCG^C
DraIII 1400 CACGTTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 2456 CCATGG C^C[GA][CT]GG
EcoRI 1528 GAATTC G^AATTC
HhaI 2569 GCGC GCG^C
HinP1I 2569 GCGC G^CGC
HincII 2399 GTCAAC GT[CT]^[GA]AC
HindII 2399 GTCAAC GT[CT]^[GA]AC
KpnI 429 GGTACC GGTAC^C
MamI 132 GATTCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1952 CAATTG C^AATTG
MluNI 623 TGGCCA TGG^CCA
MscI 623 TGGCCA TGG^CCA
MseI 371 TTAA T^TAA
MunI 1952 CAATTG C^AATTG
NcoI 2456 CCATGG C^CATGG
PaeR7I 251 CTCGAG C^TCGAG
PflFI 2009 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 2247 CCACTCAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 2071 AGGTCCC [GA]G^G[AT]CC[CT]
PshBI 370 ATTAAT AT^TAAT
SacI 1861 GAGCTC GAGCT^C
SspI 333 AATATT AAT^ATT
StuI 2093 AGGCCT AGG^CCT
Tru9I 371 TTAA T^TAA
Tth111I 2009 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 2247 CCACTCAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 698 CCAGAAAGAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 251 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 24 enzymes) #
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AflIII 618 ACATGT A^C[GA][CT]GT
2403 ACATGT
AocI 1831 CCTCAGG CC^T[ACGT]AGG
2067 CCTAAGG
Asp700I 486 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1142 GAAAGAGTTC
AvaI 251 CTCGAG C^[CT]CG[GA]G
1820 CCCGAG
BsaI 379 GAGACC GGTCTC(1/5)
2452 GAGACC
BsaWI 1576 ACCGGA [AT]^CCGG[AT]
1597 TCCGGT
BsoBI 251 CTCGAG C^[CT]CG[GA]G
1820 CCCGAG
Bsp1407I 1589 TGTACA T^GTACA
1898 TGTACA
BspHI 1632 TCATGA T^CATGA
1985 TCATGA
BspLU11I 618 ACATGT A^CATGT
2403 ACATGT
BspMI 1325 ACCTGC ACCTGC(4/8)
1970 ACCTGC
BsrGI 1589 TGTACA T^GTACA
1898 TGTACA
BstBI 214 TTCGAA TT^CGAA
972 TTCGAA
Bsu36I 1831 CCTCAGG CC^T[ACGT]AGG
2067 CCTAAGG
DraII 55 AGGCCCT [GA]G^G[ACGT]CC[CT]
2071 AGGTCCC
Eco81I 1831 CCTCAGG CC^T[ACGT]AGG
2067 CCTAAGG
EcoO109I 55 AGGCCCT [GA]G^G[ACGT]CC[CT]
2071 AGGTCCC
MspA1I 2053 CAGCTG C[AC]G^C[GT]G
2116 CAGCTG
NspV 214 TTCGAA TT^CGAA
972 TTCGAA
PvuII 2053 CAGCTG CAG^CTG
2116 CAGCTG
RcaI 1632 TCATGA T^CATGA
1985 TCATGA
SfuI 214 TTCGAA TT^CGAA
972 TTCGAA
SspBI 1589 TGTACA T^GTACA
1898 TGTACA
XmnI 486 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1142 GAAAGAGTTC
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# 3 OR MORE CUT ENZYMES ( 88 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AspHI AvaII BanI BanII
BbsI BbvI BcnI BfaI BglII
BmyI BpmI BpuAI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrI BssKI BstEII
BstF5I BstNI BstPI BstXI BstYI
Cac8I Cfr13I DdeI DpnII EaeI
EarI EcoNI EcoO65I EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MslI MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI PstI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI TfiI TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII