(Circular) (Six-base) MAPSORT of: pFN21AB8496.seq Check: 4862 from: 1 to: 7288 pFN21AB8496 7288 bp With 182 enzymes: SgfI * April 15, 2010 09:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5098 278 Size: 53 83 186 936 3562 2468 Fragments arranged by size: 3562 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4631 1937 Size: 2694 4594 Acc65I G'GTAC_C Cuts at: 2434 4609 2434 Size: 2175 5113 AclI AA'CG_TT Cuts at: 1563 5415 5788 1563 Size: 3852 373 3063 Fragments arranged by size: 3852 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2048 3024 5351 6735 7003 1632 Size: 416 976 2327 1384 268 1917 Fragments arranged by size: 2327 1917 1384 976 416 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7091 AflIII A'CryG_T Cuts at: 1203 2623 4408 6187 7164 1203 Size: 1420 1785 1779 977 1327 Fragments arranged by size: 1785 1779 1420 1327 977 AgeI A'CCGG_T Cuts at: 6119 6119 Size: 7288 AhdI GACnn_n'nnGTC Cuts at: 6017 6017 Size: 7288 AleI CACnn'nnGTG Cuts at: 1166 3371 4165 1166 Size: 2205 794 4289 Fragments arranged by size: 4289 2205 794 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2575 3513 4183 1184 Size: 1391 938 670 4289 Fragments arranged by size: 4289 1391 938 670 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3519 3551 3989 4021 4317 4349 3519 Size: 32 438 32 296 32 6458 Fragments arranged by size: 6458 438 296 32 32 32 AlwNI CAG_nnn'CTG Cuts at: 5031 6603 5031 Size: 1572 5716 ApaLI G'TGCA_C Cuts at: 2328 5345 6501 2328 Size: 3017 1156 3115 Fragments arranged by size: 3115 3017 1156 ApoI r'AATT_y Cuts at: 1493 2495 3533 4309 4597 4823 4975 7084 1493 Size: 1002 1038 776 288 226 152 2109 1697 Fragments arranged by size: 2109 1697 1038 1002 776 288 226 152 AseI AT'TA_AT Cuts at: 160 1032 2376 5842 160 Size: 872 1344 3466 1606 Fragments arranged by size: 3466 1606 1344 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7288 AvaI C'yCGr_G Cuts at: 1870 1943 2256 3825 4613 1870 Size: 73 313 1569 788 4545 Fragments arranged by size: 4545 1569 788 313 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 4424 4457 4424 Size: 33 7255 BamHI G'GATC_C Cuts at: 4618 4985 4618 Size: 367 6921 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2434 3046 3373 4167 4609 Size: 325 217 627 647 612 327 794 442 Cuts at: 4609 6064 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 794 647 627 612 442 327 325 217 BanII G_rGCy'C Cuts at: 1391 2603 3732 3870 4487 4512 4607 1391 Size: 1212 1129 138 617 25 95 4072 Fragments arranged by size: 4072 1212 1129 617 138 95 25 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2036 2465 3629 928 Size: 971 137 429 1164 4587 Fragments arranged by size: 4587 1164 971 429 137 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3170 3204 5479 5513 1903 Size: 34 1233 34 2275 34 3678 Fragments arranged by size: 3678 2275 1233 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3149 5180 6390 3149 Size: 2031 1210 4047 Fragments arranged by size: 4047 2031 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 7288 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5899 136 Size: 107 122 71 631 605 4227 1525 Fragments arranged by size: 4227 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2834 3035 4197 7283 2834 Size: 201 1162 3086 2839 Fragments arranged by size: 3086 2839 1162 201 BlpI GC'TnA_GC Cuts at: 4705 4705 Size: 7288 Bme1580I G_kGCm'C Cuts at: 1790 2332 5349 6505 1790 Size: 542 3017 1156 2573 Fragments arranged by size: 3017 2573 1156 542 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7288 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4025 5019 5977 Size: 416 53 83 399 24 2608 994 958 Cuts at: 5977 442 Size: 1753 Fragments arranged by size: 2608 1753 994 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7288 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2836 4364 2836 Size: 1528 5760 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2735 2763 2850 3488 4173 4394 5948 1239 Size: 1496 28 87 638 685 221 1554 2579 Fragments arranged by size: 2579 1554 1496 685 638 221 87 28 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2231 3374 4070 5413 6278 6576 2231 Size: 1143 696 1343 865 298 2943 Fragments arranged by size: 2943 1343 1143 865 696 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2378 4451 5951 882 Size: 617 879 2073 1500 2219 Fragments arranged by size: 2219 2073 1500 879 617 BsaAI yAC'GTr Cuts at: 493 1204 5079 493 Size: 711 3875 2702 Fragments arranged by size: 3875 2702 711 BsaBI GATnn'nnATC Cuts at: 2141 7282 2141 Size: 5141 2147 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5095 5477 6112 275 Size: 53 83 186 936 3562 382 635 1451 Fragments arranged by size: 3562 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3581 3602 5721 6119 6393 6540 6951 3581 Size: 21 2119 398 274 147 411 3918 Fragments arranged by size: 3918 2119 411 398 274 147 21 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2642 2672 2848 2878 3112 Size: 30 165 30 1239 30 176 30 234 Cuts at: 3112 3142 4469 4499 1178 Size: 30 1327 30 3967 Fragments arranged by size: 3967 1327 1239 234 176 165 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2229 3014 3408 1186 Size: 1043 785 394 5066 Fragments arranged by size: 5066 1043 785 394 BseYI C'CCAG_C Cuts at: 2614 2748 4212 4511 6491 6987 2614 Size: 134 1464 299 1980 496 2915 Fragments arranged by size: 2915 1980 1464 496 299 134 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2066 2571 2613 3805 4441 1163 Size: 573 330 505 42 1192 636 4010 Fragments arranged by size: 4010 1192 636 573 505 330 42 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4738 5499 6527 1540 Size: 275 186 2737 761 1028 2301 Fragments arranged by size: 2737 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2257 2332 3310 3870 5349 5434 6505 2257 Size: 75 978 560 1479 85 1071 3040 Fragments arranged by size: 3040 1479 1071 978 560 85 75 BsmI GAATG_Cn' Cuts at: 4799 4892 4799 Size: 93 7195 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2257 2332 2603 3310 3732 3870 4487 Size: 399 467 75 271 707 422 138 617 Cuts at: 4487 4512 4607 5349 5434 6505 1391 Size: 25 95 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 707 617 467 422 399 271 138 95 85 75 25 BspEI T'CCGG_A Cuts at: 6951 6951 Size: 7288 BspHI T'CATG_A Cuts at: 3637 3990 5177 6907 3637 Size: 353 1187 1730 4018 Fragments arranged by size: 4018 1730 1187 353 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3339 3984 4643 844 Size: 2495 645 659 3489 Fragments arranged by size: 3489 2495 659 645 BsrBI CCG'CTC Cuts at: 1394 1823 3416 5175 1394 Size: 429 1593 1759 3507 Fragments arranged by size: 3507 1759 1593 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5783 5957 65 Size: 1183 4535 174 1396 Fragments arranged by size: 4535 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5932 6119 1700 Size: 110 4122 187 2869 Fragments arranged by size: 4122 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3594 3903 96 Size: 3498 309 3481 Fragments arranged by size: 3498 3481 309 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7288 BssSI C'ACGA_G Cuts at: 5348 6360 5348 Size: 1012 6276 BstAPI GCAn_nnn'nTGC Cuts at: 794 3754 794 Size: 2960 4328 BstBI TT'CG_AA Cuts at: 2220 2978 6092 2220 Size: 758 3114 3416 Fragments arranged by size: 3416 3114 758 BstEII G'GTnAC_C Cuts at: 1184 2732 3831 4240 1184 Size: 1548 1099 409 4232 Fragments arranged by size: 4232 1548 1099 409 BstXI CCAn_nnnn'nTGG Cuts at: 3466 3970 4015 4123 3466 Size: 504 45 108 6631 Fragments arranged by size: 6631 504 108 45 BstYI r'GATC_y Cuts at: 1972 2834 3035 3423 4068 4197 4618 4985 5370 Size: 862 201 388 645 129 421 367 385 Cuts at: 5370 5387 6839 6925 6937 7283 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 862 645 421 388 385 367 346 201 129 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3837 4073 3837 Size: 236 7052 BtgI C'CryG_G Cuts at: 513 1065 2004 4461 513 Size: 552 939 2457 3340 Fragments arranged by size: 3340 2457 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2930 487 Size: 683 56 1704 4845 Fragments arranged by size: 4845 1704 683 56 BtsI GCAGTG_nn' Cuts at: 3271 4804 5598 5618 3271 Size: 1533 794 20 4941 Fragments arranged by size: 4941 1533 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7288 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2412 2447 529 Size: 35 1848 35 5370 Fragments arranged by size: 5370 1848 35 35 DraI TTT'AAA Cuts at: 4592 4592 Size: 7288 DraIII CAC_nnn'GTG Cuts at: 3410 3410 Size: 7288 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6295 809 Size: 507 4979 1802 Fragments arranged by size: 4979 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2628 4299 4464 Size: 54 1425 50 275 18 798 1671 165 Cuts at: 4464 4735 5624 7253 8 Size: 271 889 1629 43 Fragments arranged by size: 1671 1629 1425 889 798 275 271 165 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4735 1537 Size: 275 2923 4090 Fragments arranged by size: 4090 2923 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3216 3270 3377 3519 5218 1353 Size: 1863 54 107 142 1699 3423 Fragments arranged by size: 3423 1863 1699 142 107 54 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2644 5875 6249 6395 387 Size: 1064 138 1055 3231 374 146 1280 Fragments arranged by size: 3231 1280 1064 1055 374 146 138 EcoICRI GAG'CTC Cuts at: 3868 3868 Size: 7288 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2048 2735 2763 2850 3024 3488 4173 Size: 393 416 687 28 87 174 464 685 Cuts at: 4173 4394 5351 5948 6735 7003 1239 Size: 221 957 597 787 268 1524 Fragments arranged by size: 1524 957 787 687 685 597 464 416 393 268 221 174 87 28 EcoNI CCTnn'n_nnAGG Cuts at: 3368 3615 3659 3368 Size: 247 44 6997 Fragments arranged by size: 6997 247 44 EcoO109I rG'GnC_Cy Cuts at: 2061 4077 2061 Size: 2016 5272 EcoRI G'AATT_C Cuts at: 3533 4597 4975 3533 Size: 1064 378 5846 Fragments arranged by size: 5846 1064 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2482 3370 2482 Size: 888 6400 FspI TGC'GCA Cuts at: 5794 5794 Size: 7288 HaeII r_GCGC'y Cuts at: 1678 6435 1678 Size: 4757 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3112 3144 3154 3186 3415 Size: 32 135 32 1540 32 10 32 229 Cuts at: 3415 3447 4387 4419 5929 5961 6003 6035 1373 Size: 32 940 32 1510 32 42 32 2626 Fragments arranged by size: 2626 1540 1510 940 229 135 42 32 32 32 32 32 32 32 32 10 HincII GTy'rAC Cuts at: 669 1657 1938 4406 4632 4878 669 Size: 988 281 2468 226 246 3079 Fragments arranged by size: 3079 2468 988 281 246 226 HindIII A'AGCT_T Cuts at: 748 748 Size: 7288 HpaI GTT'AAC Cuts at: 4878 4878 Size: 7288 KpnI G_GTAC'C Cuts at: 2438 4613 2438 Size: 2175 5113 MfeI C'AATT_G Cuts at: 3957 4887 3957 Size: 930 6358 MluI A'CGCG_T Cuts at: 7164 7164 Size: 7288 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2049 6402 6586 1305 Size: 744 4353 184 2007 Fragments arranged by size: 4353 2007 744 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2630 10 Size: 54 1425 343 798 4668 Fragments arranged by size: 4668 1425 798 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3371 4165 4466 5246 5605 5764 518 Size: 648 2205 794 301 780 359 159 2042 Fragments arranged by size: 2205 2042 794 780 648 359 301 159 MspA1I CmG'CkG Cuts at: 1606 4060 4123 4704 5381 6529 6774 1606 Size: 2454 63 581 677 1148 245 2120 Fragments arranged by size: 2454 2120 1148 677 581 245 63 NcoI C'CATG_G Cuts at: 513 1065 2004 4461 513 Size: 552 939 2457 3340 Fragments arranged by size: 3340 2457 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7288 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7288 NotI GC'GGCC_GC Cuts at: 4735 4735 Size: 7288 NspI r_CATG'y Cuts at: 1132 1229 2627 3494 3595 4412 4646 6191 1132 Size: 97 1398 867 101 817 234 1545 2229 Fragments arranged by size: 2229 1545 1398 867 817 234 101 97 PasI CC'CwG_GG Cuts at: 1348 3611 1348 Size: 2263 5025 PciI A'CATG_T Cuts at: 2623 4408 6187 2623 Size: 1785 1779 3724 Fragments arranged by size: 3724 1785 1779 PflMI CCAn_nnn'nTGG Cuts at: 1108 4258 1108 Size: 3150 4138 PfoI T'CCnGG_A Cuts at: 2480 2779 3323 2480 Size: 299 544 6445 Fragments arranged by size: 6445 544 299 PmeI GTTT'AAAC Cuts at: 4592 4592 Size: 7288 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5357 5389 6870 6902 5357 Size: 32 1481 32 5743 Fragments arranged by size: 5743 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 4077 4077 Size: 7288 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7288 PsiI TTA'TAA Cuts at: 4858 4858 Size: 7288 PspXI vC'TCGA_Gb Cuts at: 2256 2256 Size: 7288 PstI C_TGCA'G Cuts at: 830 3660 4065 4184 4640 830 Size: 2830 405 119 456 3478 Fragments arranged by size: 3478 2830 456 405 119 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7288 PvuII CAG'CTG Cuts at: 4060 4123 4060 Size: 63 7225 SacI G_AGCT'C Cuts at: 3870 3870 Size: 7288 SalI G'TCGA_C Cuts at: 1936 4630 1936 Size: 2694 4594 SbfI CC_TGCA'GG Cuts at: 4640 4640 Size: 7288 ScaI AGT'ACT Cuts at: 5536 7248 5536 Size: 1712 5576 SfcI C'TryA_G Cuts at: 826 1045 2095 3656 4061 4180 4636 5771 6452 Size: 219 1050 1561 405 119 456 1135 681 Cuts at: 6452 6643 826 Size: 191 1471 Fragments arranged by size: 1561 1471 1135 1050 681 456 405 219 191 119 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7288 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7288 SmaI CCC'GGG Cuts at: 1872 4615 1872 Size: 2743 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2210 2256 3389 4085 5392 6293 Size: 197 926 267 46 1133 696 1307 901 Cuts at: 6293 6555 820 Size: 262 1553 Fragments arranged by size: 1553 1307 1133 926 901 696 267 262 197 46 SnaBI TAC'GTA Cuts at: 493 5079 493 Size: 4586 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7288 SphI G_CATG'C Cuts at: 1132 4646 1132 Size: 3514 3774 SspI AAT'ATT Cuts at: 5 52 2340 5212 5 Size: 47 2288 2872 2081 Fragments arranged by size: 2872 2288 2081 47 StuI AGG'CCT Cuts at: 1517 4100 1517 Size: 2583 4705 StyI C'CwwG_G Cuts at: 513 1065 2004 2769 3299 4461 4727 513 Size: 552 939 765 530 1162 266 3074 Fragments arranged by size: 3074 1162 939 765 552 530 266 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3325 5315 5332 5485 5670 6115 325 Size: 1255 1745 1990 17 153 185 445 1498 Fragments arranged by size: 1990 1745 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2260 3594 3903 Size: 274 80 33 51 1443 283 1334 309 Cuts at: 3903 5534 7246 96 Size: 1631 1712 138 Fragments arranged by size: 1712 1631 1443 1334 309 283 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2145 2177 2311 2343 2642 2674 4469 4501 7248 Size: 32 134 32 299 32 1795 32 2747 Cuts at: 7248 7280 2145 Size: 32 2153 Fragments arranged by size: 2747 2153 1795 299 134 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 4017 1316 Size: 2701 4587 XbaI T'CTAG_A Cuts at: 4624 4624 Size: 7288 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2710 2710 Size: 7288 XhoI C'TCGA_G Cuts at: 1943 2256 1943 Size: 313 6975 XmaI C'CCGG_G Cuts at: 1870 4613 1870 Size: 2743 4545 XmnI GAAnn'nnTTC Cuts at: 2495 3151 5417 2495 Size: 656 2266 4366 Fragments arranged by size: 4366 2266 656 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5096 276 Size: 53 83 186 936 3562 2468 Fragments arranged by size: 3562 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI