Restriction map for
pFN21AB8496
(Linear) (Six-base) MAPSORT of: KIBB8496.seq Check: 5263 from: 1 to: 2583
KIBB8496.orf
With 182 enzymes: SgfI *
October 7, 2009 14:25 ..
Acc65I G'GTAC_C
Cuts at: 0 429 2583
Size: 429 2154
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 43 1019 2583
Size: 43 976 1564
Fragments arranged by size:
1564 976 43
AflIII A'CryG_T
Cuts at: 0 618 2403 2583
Size: 618 1785 180
Fragments arranged by size:
1785 618 180
AleI CACnn'nnGTG
Cuts at: 0 1366 2160 2583
Size: 1366 794 423
Fragments arranged by size:
1366 794 423
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 570 1508 2178 2583
Size: 570 938 670 405
Fragments arranged by size:
938 670 570 405
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1514 1546 1984 2016 2312 2344 2583
Size: 1514 32 438 32 296 32 239
Fragments arranged by size:
1514 438 296 239 32 32 32
ApaLI G'TGCA_C
Cuts at: 0 323 2583
Size: 323 2260
ApoI r'AATT_y
Cuts at: 0 490 1528 2304 2583
Size: 490 1038 776 279
Fragments arranged by size:
1038 776 490 279
AseI AT'TA_AT
Cuts at: 0 371 2583
Size: 371 2212
AvaI C'yCGr_G
Cuts at: 0 251 1820 2583
Size: 251 1569 763
Fragments arranged by size:
1569 763 251
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 2419 2452 2583
Size: 2419 33 131
Fragments arranged by size:
2419 131 33
BanI G'GyrC_C
Cuts at: 0 429 1041 1368 2162 2583
Size: 429 612 327 794 421
Fragments arranged by size:
794 612 429 421 327
BanII G_rGCy'C
Cuts at: 0 598 1727 1865 2482 2507 2583
Size: 598 1129 138 617 25 76
Fragments arranged by size:
1129 617 598 138 76 25
BbsI GAAGACnn'nnnn_
Cuts at: 0 31 460 1624 2583
Size: 31 429 1164 959
Fragments arranged by size:
1164 959 429 31
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1165 1199 2583
Size: 1165 34 1384
Fragments arranged by size:
1384 1165 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1144 2583
Size: 1144 1439
BglII A'GATC_T
Cuts at: 0 829 1030 2192 2583
Size: 829 201 1162 391
Fragments arranged by size:
1162 829 391 201
Bme1580I G_kGCm'C
Cuts at: 0 327 2583
Size: 327 2256
BmrI ACTGGGnnnn_n'
Cuts at: 0 2020 2583
Size: 2020 563
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 831 2359 2583
Size: 831 1528 224
Fragments arranged by size:
1528 831 224
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 730 758 845 1483 2168 2389 2583
Size: 730 28 87 638 685 221 194
Fragments arranged by size:
730 685 638 221 194 87 28
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 226 1369 2065 2583
Size: 226 1143 696 518
Fragments arranged by size:
1143 696 518 226
BsaI GGTCTCn'nnnn_
Cuts at: 0 373 2446 2583
Size: 373 2073 137
Fragments arranged by size:
2073 373 137
BsaBI GATnn'nnATC
Cuts at: 0 136 2583
Size: 136 2447
BsaWI w'CCGG_w
Cuts at: 0 1576 1597 2583
Size: 1576 21 986
Fragments arranged by size:
1576 986 21
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 637 667 843 873 1107 1137 2464 2494
Size: 637 30 176 30 234 30 1327 30
Cuts at: 2494 2583
Size: 89
Fragments arranged by size:
1327 637 234 176 89 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 224 1009 1403 2583
Size: 224 785 394 1180
Fragments arranged by size:
1180 785 394 224
BseYI C'CCAG_C
Cuts at: 0 609 743 2207 2506 2583
Size: 609 134 1464 299 77
Fragments arranged by size:
1464 609 299 134 77
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 61 566 608 1800 2436 2583
Size: 61 505 42 1192 636 147
Fragments arranged by size:
1192 636 505 147 61 42
BsiHKAI G_wGCw'C
Cuts at: 0 252 327 1305 1865 2583
Size: 252 75 978 560 718
Fragments arranged by size:
978 718 560 252 75
Bsp1286I G_dGCh'C
Cuts at: 0 252 327 598 1305 1727 1865 2482 2507
Size: 252 75 271 707 422 138 617 25
Cuts at: 2507 2583
Size: 76
Fragments arranged by size:
707 617 422 271 252 138 76 75
25
BspHI T'CATG_A
Cuts at: 0 1632 1985 2583
Size: 1632 353 598
Fragments arranged by size:
1632 598 353
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1334 1979 2583
Size: 1334 645 604
Fragments arranged by size:
1334 645 604
BsrBI CCG'CTC
Cuts at: 0 1411 2583
Size: 1411 1172
BsrGI T'GTAC_A
Cuts at: 0 1589 1898 2583
Size: 1589 309 685
Fragments arranged by size:
1589 685 309
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1749 2583
Size: 1749 834
BstBI TT'CG_AA
Cuts at: 0 215 973 2583
Size: 215 758 1610
Fragments arranged by size:
1610 758 215
BstEII G'GTnAC_C
Cuts at: 0 727 1826 2235 2583
Size: 727 1099 409 348
Fragments arranged by size:
1099 727 409 348
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1461 1965 2010 2118 2583
Size: 1461 504 45 108 465
Fragments arranged by size:
1461 504 465 108 45
BstYI r'GATC_y
Cuts at: 0 829 1030 1418 2063 2192 2583
Size: 829 201 388 645 129 391
Fragments arranged by size:
829 645 391 388 201 129
Bsu36I CC'TnA_GG
Cuts at: 0 1832 2068 2583
Size: 1832 236 515
Fragments arranged by size:
1832 515 236
BtgI C'CryG_G
Cuts at: 0 2456 2583
Size: 2456 127
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 925 2583
Size: 925 1658
BtsI GCAGTG_nn'
Cuts at: 0 1266 2583
Size: 1266 1317
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 407 442 2583
Size: 407 35 2141
Fragments arranged by size:
2141 407 35
DraIII CAC_nnn'GTG
Cuts at: 0 1405 2583
Size: 1405 1178
EaeI y'GGCC_r
Cuts at: 0 623 2294 2459 2583
Size: 623 1671 165 124
Fragments arranged by size:
1671 623 165 124
EarI CTCTTCn'nnn_
Cuts at: 0 1211 1265 1372 1514 2583
Size: 1211 54 107 142 1069
Fragments arranged by size:
1211 1069 142 107 54
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 639 2583
Size: 639 1944
EcoICRI GAG'CTC
Cuts at: 0 1863 2583
Size: 1863 720
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 43 730 758 845 1019 1483 2168 2389
Size: 43 687 28 87 174 464 685 221
Cuts at: 2389 2583
Size: 194
Fragments arranged by size:
687 685 464 221 194 174 87 43
28
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1363 1610 1654 2583
Size: 1363 247 44 929
Fragments arranged by size:
1363 929 247 44
EcoO109I rG'GnC_Cy
Cuts at: 0 56 2072 2583
Size: 56 2016 511
Fragments arranged by size:
2016 511 56
EcoRI G'AATT_C
Cuts at: 0 1528 2583
Size: 1528 1055
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 477 1365 2583
Size: 477 888 1218
Fragments arranged by size:
1218 888 477
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1107 1139 1149 1181 1410 1442 2382 2414
Size: 1107 32 10 32 229 32 940 32
Cuts at: 2414 2583
Size: 169
Fragments arranged by size:
1107 940 229 169 32 32 32 32
10
HincII GTy'rAC
Cuts at: 0 2401 2583
Size: 2401 182
KpnI G_GTAC'C
Cuts at: 0 433 2583
Size: 433 2150
MfeI C'AATT_G
Cuts at: 0 1952 2583
Size: 1952 631
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 44 2583
Size: 44 2539
MscI TGG'CCA
Cuts at: 0 625 2583
Size: 625 1958
MslI CAynn'nnrTG
Cuts at: 0 1366 2160 2461 2583
Size: 1366 794 301 122
Fragments arranged by size:
1366 794 301 122
MspA1I CmG'CkG
Cuts at: 0 2055 2118 2583
Size: 2055 63 465
Fragments arranged by size:
2055 465 63
NcoI C'CATG_G
Cuts at: 0 2456 2583
Size: 2456 127
NspI r_CATG'y
Cuts at: 0 622 1489 1590 2407 2583
Size: 622 867 101 817 176
Fragments arranged by size:
867 817 622 176 101
PasI CC'CwG_GG
Cuts at: 0 1606 2583
Size: 1606 977
PciI A'CATG_T
Cuts at: 0 618 2403 2583
Size: 618 1785 180
Fragments arranged by size:
1785 618 180
PflMI CCAn_nnn'nTGG
Cuts at: 0 2253 2583
Size: 2253 330
PfoI T'CCnGG_A
Cuts at: 0 475 774 1318 2583
Size: 475 299 544 1265
Fragments arranged by size:
1265 544 475 299
PpuMI rG'GwC_Cy
Cuts at: 0 2072 2583
Size: 2072 511
PspXI vC'TCGA_Gb
Cuts at: 0 251 2583
Size: 251 2332
PstI C_TGCA'G
Cuts at: 0 1655 2060 2179 2583
Size: 1655 405 119 404
Fragments arranged by size:
1655 405 404 119
PvuII CAG'CTG
Cuts at: 0 2055 2118 2583
Size: 2055 63 465
Fragments arranged by size:
2055 465 63
SacI G_AGCT'C
Cuts at: 0 1865 2583
Size: 1865 718
SfcI C'TryA_G
Cuts at: 0 90 1651 2056 2175 2583
Size: 90 1561 405 119 408
Fragments arranged by size:
1561 408 405 119 90
SmlI C'TyrA_G
Cuts at: 0 205 251 1384 2080 2583
Size: 205 46 1133 696 503
Fragments arranged by size:
1133 696 503 205 46
SspI AAT'ATT
Cuts at: 0 335 2583
Size: 335 2248
StuI AGG'CCT
Cuts at: 0 2095 2583
Size: 2095 488
StyI C'CwwG_G
Cuts at: 0 764 1294 2456 2583
Size: 764 530 1162 127
Fragments arranged by size:
1162 764 530 127
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1320 2583
Size: 1320 1263
TatI w'GTAC_w
Cuts at: 0 255 1589 1898 2583
Size: 255 1334 309 685
Fragments arranged by size:
1334 685 309 255
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 140 172 306 338 637 669 2464 2496
Size: 140 32 134 32 299 32 1795 32
Cuts at: 2496 2583
Size: 87
Fragments arranged by size:
1795 299 140 134 87 32 32 32
32
Tth111I GACn'n_nGTC
Cuts at: 0 2012 2583
Size: 2012 571
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 705 2583
Size: 705 1878
XhoI C'TCGA_G
Cuts at: 0 251 2583
Size: 251 2332
XmnI GAAnn'nnTTC
Cuts at: 0 490 1146 2583
Size: 490 656 1437
Fragments arranged by size:
1437 656 490
Enzymes that do cut:
Acc65I AcuI AflIII AleI AlfI AloI ApaLI ApoI
AseI AvaI BaeI BanI BanII BbsI BcgI BciVI
BglII Bme1580I BmrI BplI BpmI BpuEI BsaI BsaBI
BsaWI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspHI
BspMI BsrBI BsrGI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI CspCI DraIII EaeI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI Hin4I
HincII KpnI MfeI MmeI MscI MslI MspA1I NcoI
NspI PasI PciI PflMI PfoI PpuMI PspXI PstI
PvuII SacI SfcI SmlI SspI StuI StyI TaqII
TatI TstI Tth111I XcmI XhoI XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AgeI AhdI
AlwNI ApaI AscI AsiSI AvrII BamHI BbeI BbvCI
BclI BfrBI BglI BlpI BmgBI BmtI Bpu10I BsaAI
BsaHI BsiEI BsiWI BsmI BsmBI BspEI BsrDI BsrFI
BssHII BssSI BstZ17I ClaI DraI DrdI EagI EcoRV
FseI FspI FspAI HaeII HindIII HpaI KasI MluI
NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI
PacI PmeI PmlI PpiI PshAI PsiI PspOMI PsrI
PvuI RsrII SacII SalI SanDI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SwaI XbaI XmaI ZraI