Restriction map for
pFN21AB5764
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# NO CUT ENZYMES ( 117 enzymes) #
##################################
AatII Acc65I AclI AcyI AflII
AflIII AgeI AhdI AocI Aor51HI
ApaI AscI Asp700I Asp718I AspEI
AviII AvrII BamHI BbeI BbrPI
BbvCI BcgI BclI BfrI BglI
BlnI BlpI Bpu1102I BsaAI BsaHI
BsaI BsiEI BsiWI BsmBI Bsp1407I
BspDI BspHI BspLU11I BsrDI BsrFI
BsrGI BssHII Bst1107I BstAPI BstBI
BstEII BstPI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO65I EcoRI EcoRV FbaI
FseI FspI HaeII HgaI Hin1I
HpaI KasI KpnI KspI MaeII
MfeI MluI MunI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NspI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PshAI
Psp1406I PvuI RcaI RsrII SacI
SacII SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SnaBI
SphI Sse8387I SspBI SwaI XbaI
XhoI XmnI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AccI 2082 GTCGAC GT^[AC][GT]AC
AccII 57 CGCG CG^CG
AccIII 2057 TCCGGA T^CCGGA
AcsI 524 AAATTT [GA]^AATT[CT]
Alw44I 919 GTGCAC G^TGCAC
ApaLI 919 GTGCAC G^TGCAC
ApoI 524 AAATTT [GA]^AATT[CT]
AseI 1537 ATTAAT AT^TAAT
AsnI 1537 ATTAAT AT^TAAT
AspHI 919 GTGCAC G[AT]GC[AT]^C
AspI 1098 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 1163 CCCGGG C^[CT]CG[GA]G
BalI 311 TGGCCA TGG^CCA
BanI 193 GGCACC G^G[CT][GA]CC
BanII 1005 GGGCTC G[GA]GC[CT]^C
BbsI 1104 GTCTTC GAAGAC(2/6)
BciVI 1347 GGATAC GTATCC(6/5)
BglII 213 AGATCT A^GATCT
BpuAI 1104 GTCTTC GAAGAC(2/6)
BsaBI 174 GATTCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 2057 TCCGGA [AT]^CCGG[AT]
BseAI 2057 TCCGGA T^CCGGA
BsiHKAI 919 GTGCAC G[AT]GC[AT]^C
BsmI 2035 GAATGC GAATGC(1/-1)
BsoBI 1163 CCCGGG C^[CT]CG[GA]G
BspEI 2057 TCCGGA T^CCGGA
BstUI 57 CGCG CG^CG
DraI 495 TTTAAA TTT^AAA
DsaI 635 CCGTGG C^C[GA][CT]GG
EcoNI 948 CCTGCACGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 1616 CCTTGG C^C[AT][AT]GG
EcoT22I 1181 ATGCAT ATGCA^T
HincII 2082 GTCGAC GT[CT]^[GA]AC
HindII 2082 GTCGAC GT[CT]^[GA]AC
MamI 174 GATTCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 311 TGGCCA TGG^CCA
MroI 2057 TCCGGA T^CCGGA
MscI 311 TGGCCA TGG^CCA
MslI 1983 CACTGAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 57 CGCG CG^CG
NsiI 1181 ATGCAT ATGCA^T
PflFI 1098 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 1537 ATTAAT AT^TAAT
PvuII 356 CAGCTG CAG^CTG
SalI 2082 GTCGAC G^TCGAC
SmaI 1163 CCCGGG CCC^GGG
SmlI 813 CTTGAG C^T[CT][GA]AG
SspI 643 AATATT AAT^ATT
StuI 162 AGGCCT AGG^CCT
StyI 1616 CCTTGG C^C[AT][AT]GG
Tsp45I 1234 GTCAC ^GT[GC]AC
Tth111I 1098 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 1777 CCAGAGAGTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 1163 CCCGGG C^CCGGG
XmaI 1163 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
BsgI 949 CTGCAC GTGCAG(16/14)
976 GTGCAG
BspMI 2234 ACCTGC ACCTGC(4/8)
542 GCAGGT
BsrBI 1741 CCGCTC CCGCTC(-3/-3)
970 GAGCGG
BsrI 275 CCAGT ACTGG(1/-1)
1214 CCAGT
BssSI 480 CACGAG CACGAG(-5/-1)
952 CACGAG
BstXI 183 CCAAGACAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2125 CCAAACTCCTGG
BstYI 179 AGATCC [GA]^GATC[CT]
213 AGATCT
CfoI 2039 GCGC GCG^C
2172 GCGC
EaeI 79 CGGCCA [CT]^GGCC[GA]
311 TGGCCA
EarI 778 CTCTTC CTCTTC(1/4)
1380 GAAGAG
FauI 61 GCGGG CCCGC(4/6)
972 GCGGG
HhaI 2039 GCGC GCG^C
2172 GCGC
HinP1I 2039 GCGC G^CGC
2172 GCGC
Ksp632I 778 CTCTTC CTCTTC(1/4)
1380 GAAGAG
MflI 179 AGATCC [GA]^GATC[CT]
213 AGATCT
MspA1I 76 CAGCGG C[AC]G^C[GT]G
356 CAGCTG
PleI 1521 GAGTC GAGTC(4/5)
2080 GAGTC
SpeI 244 ACTAGT A^CTAGT
1510 ACTAGT
XhoII 179 AGATCC [GA]^GATC[CT]
213 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 67 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AvaII BbvI BcnI BfaI BmyI
BpmI BsaJI BseRI BsiYI BslI
BsmAI BsmFI Bsp1286I BssKI BstF5I
BstNI Cac8I Cfr13I DdeI DpnII
DraII EcoO109I EcoRII Fnu4HI FokI
HaeIII HapII HindIII HinfI HpaII
HphI ItaI MaeI MaeIII MboI
MboII MnlI MseI MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PflMI PpuMI PstI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI TaqI
TfiI Tru9I TseI Tsp509I TspRI
TthHB8I Van91I