(Circular) (Six-base) MAPSORT of: pFN21AB5764.seq Check: 4374 from: 1 to: 6973 pFN21AB5764 6973 bp With 182 enzymes: SgfI * April 15, 2010 09:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4783 278 Size: 53 83 186 936 3247 2468 Fragments arranged by size: 3247 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4088 4316 1937 Size: 2151 228 4594 Fragments arranged by size: 4594 2151 228 Acc65I G'GTAC_C Cuts at: 4294 4294 Size: 6973 AclI AA'CG_TT Cuts at: 1563 5100 5473 1563 Size: 3537 373 3063 Fragments arranged by size: 3537 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2192 3339 3354 3869 5036 6420 6688 1632 Size: 560 1147 15 515 1167 1384 268 1917 Fragments arranged by size: 1917 1384 1167 1147 560 515 268 15 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6776 AflIII A'CryG_T Cuts at: 1203 5872 6849 1203 Size: 4669 977 1327 Fragments arranged by size: 4669 1327 977 AgeI A'CCGG_T Cuts at: 5804 5804 Size: 6973 AhdI GACnn_n'nnGTC Cuts at: 5702 5702 Size: 6973 AleI CACnn'nnGTG Cuts at: 1166 3992 1166 Size: 2826 4147 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6973 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3546 3578 3546 Size: 32 6941 AlwNI CAG_nnn'CTG Cuts at: 2609 3202 3314 4716 6288 2609 Size: 593 112 1402 1572 3294 Fragments arranged by size: 3294 1572 1402 593 112 ApaLI G'TGCA_C Cuts at: 2924 5030 6186 2924 Size: 2106 1156 3711 Fragments arranged by size: 3711 2106 1156 ApoI r'AATT_y Cuts at: 1493 2529 4282 4508 4660 6769 1493 Size: 1036 1753 226 152 2109 1697 Fragments arranged by size: 2109 1753 1697 1036 226 152 AseI AT'TA_AT Cuts at: 160 1032 3543 5527 160 Size: 872 2511 1984 1606 Fragments arranged by size: 2511 1984 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6973 AvaI C'yCGr_G Cuts at: 1870 1943 3168 4298 1870 Size: 73 1225 1130 4545 Fragments arranged by size: 4545 1225 1130 73 BamHI G'GATC_C Cuts at: 4303 4670 4303 Size: 367 6606 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2198 4294 5749 618 Size: 325 217 627 411 2096 1455 1842 Fragments arranged by size: 2096 1842 1455 627 411 325 217 BanII G_rGCy'C Cuts at: 1391 3014 4292 1391 Size: 1623 1278 4072 Fragments arranged by size: 4072 1623 1278 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3102 928 Size: 971 1203 4799 Fragments arranged by size: 4799 1203 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5164 5198 1903 Size: 34 3227 34 3678 Fragments arranged by size: 3678 3227 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3346 4865 6075 3346 Size: 1519 1210 4244 Fragments arranged by size: 4244 1519 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6973 BfrBI ATG'CAT Cuts at: 3188 3188 Size: 6973 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5584 136 Size: 107 122 71 631 605 3912 1525 Fragments arranged by size: 3912 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2218 6968 2218 Size: 4750 2223 BlpI GC'TnA_GC Cuts at: 4390 4390 Size: 6973 Bme1580I G_kGCm'C Cuts at: 1790 2928 3948 5034 6190 1790 Size: 1138 1020 1086 1156 2573 Fragments arranged by size: 2573 1156 1138 1086 1020 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6973 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4704 5662 442 Size: 416 53 83 399 24 3287 958 1753 Fragments arranged by size: 3287 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6973 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2993 3007 3515 5633 1239 Size: 1754 14 508 2118 2579 Fragments arranged by size: 2579 2118 1754 508 14 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2839 5098 5963 6261 2839 Size: 2259 865 298 3551 Fragments arranged by size: 3551 2259 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5636 882 Size: 617 4137 2219 Fragments arranged by size: 4137 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4764 493 Size: 711 3560 2702 Fragments arranged by size: 3560 2702 711 BsaBI GATnn'nnATC Cuts at: 2183 6967 2183 Size: 4784 2189 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4780 5162 5797 275 Size: 53 83 186 936 3247 382 635 1451 Fragments arranged by size: 3247 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4062 5406 5804 6078 6225 6636 4062 Size: 1344 398 274 147 411 4399 Fragments arranged by size: 4399 1344 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2589 2619 1178 Size: 30 165 30 1186 30 5532 Fragments arranged by size: 5532 1186 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2503 2975 3305 4097 1186 Size: 1317 472 330 792 4062 Fragments arranged by size: 4062 1317 792 472 330 BseYI C'CCAG_C Cuts at: 3307 6176 6672 3307 Size: 2869 496 3608 Fragments arranged by size: 3608 2869 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2939 3002 1163 Size: 573 1203 63 5134 Fragments arranged by size: 5134 1203 573 63 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4423 5184 6212 1540 Size: 275 186 2422 761 1028 2301 Fragments arranged by size: 2422 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2928 5034 5119 6190 2928 Size: 2106 85 1071 3711 Fragments arranged by size: 3711 2106 1071 85 BsmI GAATG_Cn' Cuts at: 4046 4484 4577 4046 Size: 438 93 6442 Fragments arranged by size: 6442 438 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2928 3014 3948 4292 5034 5119 6190 Size: 399 1138 86 934 344 742 85 1071 Cuts at: 6190 1391 Size: 2174 Fragments arranged by size: 2174 1138 1071 934 742 399 344 86 85 BspEI T'CCGG_A Cuts at: 4062 6636 4062 Size: 2574 4399 BspHI T'CATG_A Cuts at: 4862 6592 4862 Size: 1730 5243 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2538 4248 4328 844 Size: 1694 1710 80 3489 Fragments arranged by size: 3489 1710 1694 80 BsrBI CCG'CTC Cuts at: 1394 1823 2977 3748 4860 1394 Size: 429 1154 771 1112 3507 Fragments arranged by size: 3507 1154 1112 771 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5468 5642 65 Size: 1183 4220 174 1396 Fragments arranged by size: 4220 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5617 5804 1700 Size: 110 3807 187 2869 Fragments arranged by size: 3807 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6973 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6973 BssSI C'ACGA_G Cuts at: 2485 2957 5033 6045 2485 Size: 472 2076 1012 3413 Fragments arranged by size: 3413 2076 1012 472 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6973 BstBI TT'CG_AA Cuts at: 5777 5777 Size: 6973 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6973 BstXI CCAn_nnnn'nTGG Cuts at: 2195 4137 2195 Size: 1942 5031 BstYI r'GATC_y Cuts at: 1972 2184 2218 4303 4670 5055 5072 6524 6610 Size: 212 34 2085 367 385 17 1452 86 Cuts at: 6610 6622 6968 1972 Size: 12 346 1977 Fragments arranged by size: 2085 1977 1452 385 367 346 212 86 34 17 12 BtgI C'CryG_G Cuts at: 513 1065 2640 513 Size: 552 1575 4846 Fragments arranged by size: 4846 1575 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2044 487 Size: 683 56 818 5416 Fragments arranged by size: 5416 818 683 56 BtsI GCAGTG_nn' Cuts at: 4489 5283 5303 4489 Size: 794 20 6159 Fragments arranged by size: 6159 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6973 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6938 DraI TTT'AAA Cuts at: 2502 4277 2502 Size: 1775 5198 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5980 809 Size: 507 4664 1802 Fragments arranged by size: 4664 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2084 2316 4420 Size: 54 1425 50 275 18 254 232 2104 Cuts at: 4420 5309 6938 8 Size: 889 1629 43 Fragments arranged by size: 2104 1629 1425 889 275 254 232 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4420 1537 Size: 275 2608 4090 Fragments arranged by size: 4090 2608 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2789 3380 4903 1353 Size: 1436 591 1523 3423 Fragments arranged by size: 3423 1523 1436 591 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5560 5934 6080 387 Size: 1064 138 3971 374 146 1280 Fragments arranged by size: 3971 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2192 2993 3007 3339 3354 3515 3869 Size: 393 560 801 14 332 15 161 354 Cuts at: 3869 5036 5633 6420 6688 1239 Size: 1167 597 787 268 1524 Fragments arranged by size: 1524 1167 801 787 597 560 393 354 332 268 161 15 14 EcoNI CCTnn'n_nnAGG Cuts at: 2957 2957 Size: 6973 EcoO109I rG'GnC_Cy Cuts at: 2445 2464 3392 2445 Size: 19 928 6026 Fragments arranged by size: 6026 928 19 EcoRI G'AATT_C Cuts at: 4282 4660 4282 Size: 378 6595 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2791 3839 2791 Size: 1048 5925 FspI TGC'GCA Cuts at: 5479 5479 Size: 6973 HaeII r_GCGC'y Cuts at: 1678 6120 1678 Size: 4442 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2117 2149 3866 3898 3951 Size: 32 135 32 545 32 1717 32 53 Cuts at: 3951 3983 5614 5646 5688 5720 1373 Size: 32 1631 32 42 32 2626 Fragments arranged by size: 2626 1717 1631 545 135 53 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4089 4317 4563 669 Size: 988 281 2151 228 246 3079 Fragments arranged by size: 3079 2151 988 281 246 228 HindIII A'AGCT_T Cuts at: 748 2496 3179 3665 748 Size: 1748 683 486 4056 Fragments arranged by size: 4056 1748 683 486 HpaI GTT'AAC Cuts at: 4563 4563 Size: 6973 KpnI G_GTAC'C Cuts at: 4298 4298 Size: 6973 MfeI C'AATT_G Cuts at: 4572 4572 Size: 6973 MluI A'CGCG_T Cuts at: 6849 6849 Size: 6973 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6087 6271 1305 Size: 4782 184 2007 Fragments arranged by size: 4782 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2318 10 Size: 54 1425 343 486 4665 Fragments arranged by size: 4665 1425 486 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3992 4931 5290 5449 518 Size: 648 2826 939 359 159 2042 Fragments arranged by size: 2826 2042 939 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2083 2363 4389 5066 6214 6459 1606 Size: 477 280 2026 677 1148 245 2120 Fragments arranged by size: 2120 2026 1148 677 477 280 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6421 NdeI CA'TA_TG Cuts at: 387 387 Size: 6973 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6973 NotI GC'GGCC_GC Cuts at: 4420 4420 Size: 6973 NsiI A_TGCA'T Cuts at: 3190 3190 Size: 6973 NspI r_CATG'y Cuts at: 1132 1229 4331 5876 1132 Size: 97 3102 1545 2229 Fragments arranged by size: 3102 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6973 PciI A'CATG_T Cuts at: 5872 5872 Size: 6973 PflMI CCAn_nnn'nTGG Cuts at: 1108 2432 2800 2911 3151 3788 1108 Size: 1324 368 111 240 637 4293 Fragments arranged by size: 4293 1324 637 368 240 111 PfoI T'CCnGG_A Cuts at: 2970 3023 3531 3567 4136 2970 Size: 53 508 36 569 5807 Fragments arranged by size: 5807 569 508 53 36 PmeI GTTT'AAAC Cuts at: 4277 4277 Size: 6973 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5042 5074 6555 6587 5042 Size: 32 1481 32 5428 Fragments arranged by size: 5428 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2445 2464 3392 2445 Size: 19 928 6026 Fragments arranged by size: 6026 928 19 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6973 PsiI TTA'TAA Cuts at: 4543 4543 Size: 6973 PstI C_TGCA'G Cuts at: 830 2262 3198 3481 4230 4325 830 Size: 1432 936 283 749 95 3478 Fragments arranged by size: 3478 1432 936 749 283 95 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6973 PvuII CAG'CTG Cuts at: 2363 2363 Size: 6973 SalI G'TCGA_C Cuts at: 1936 4087 4315 1936 Size: 2151 228 4594 Fragments arranged by size: 4594 2151 228 SanDI GG'GwC_CC Cuts at: 2445 2445 Size: 6973 SbfI CC_TGCA'GG Cuts at: 4325 4325 Size: 6973 ScaI AGT'ACT Cuts at: 5221 6933 5221 Size: 1712 5261 SfcI C'TryA_G Cuts at: 826 1045 2258 3194 3477 4226 4321 5456 6137 Size: 219 1213 936 283 749 95 1135 681 Cuts at: 6137 6328 826 Size: 191 1471 Fragments arranged by size: 1471 1213 1135 936 749 681 283 219 191 95 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6973 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6973 SmaI CCC'GGG Cuts at: 1872 3170 4300 1872 Size: 1298 1130 4545 Fragments arranged by size: 4545 1298 1130 SmlI C'TyrA_G Cuts at: 820 1017 1943 2818 5077 5978 6240 820 Size: 197 926 875 2259 901 262 1553 Fragments arranged by size: 2259 1553 926 901 875 262 197 SnaBI TAC'GTA Cuts at: 493 4764 493 Size: 4271 2702 SpeI A'CTAG_T Cuts at: 152 2249 3515 152 Size: 2097 1266 3610 Fragments arranged by size: 3610 2097 1266 SphI G_CATG'C Cuts at: 1132 4331 1132 Size: 3199 3774 SspI AAT'ATT Cuts at: 5 52 2650 4897 5 Size: 47 2598 2247 2081 Fragments arranged by size: 2598 2247 2081 47 StuI AGG'CCT Cuts at: 1517 2169 1517 Size: 652 6321 StyI C'CwwG_G Cuts at: 513 1065 3621 4412 513 Size: 552 2556 791 3074 Fragments arranged by size: 3074 2556 791 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2886 3053 5000 5017 5170 5355 5800 Size: 1255 1306 167 1947 17 153 185 445 Cuts at: 5800 325 Size: 1498 Fragments arranged by size: 1947 1498 1306 1255 445 185 167 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2557 3030 4071 Size: 274 80 33 51 1443 580 473 1041 Cuts at: 4071 5219 6931 96 Size: 1148 1712 138 Fragments arranged by size: 1712 1443 1148 1041 580 473 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2859 2891 6933 6965 2859 Size: 32 4042 32 2867 Fragments arranged by size: 4042 2867 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3106 1316 Size: 1790 5183 XbaI T'CTAG_A Cuts at: 4309 4309 Size: 6973 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 3789 2011 Size: 1778 5195 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6973 XmaI C'CCGG_G Cuts at: 1870 3168 4298 1870 Size: 1298 1130 4545 Fragments arranged by size: 4545 1298 1130 XmnI GAAnn'nnTTC Cuts at: 5102 5102 Size: 6973 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4781 276 Size: 53 83 186 936 3247 2468 Fragments arranged by size: 3247 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SapI SexAI SfiI SfoI SrfI SwaI