Restriction map for
pFN21AB5763
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# NO CUT ENZYMES ( 88 enzymes) #
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AatII AccIII AclI AflII AgeI
AscI AseI AsnI Asp700I AspI
AviII AvrII BamHI BbvCI BcgI
BciVI BclI BfrI BglII BlnI
BlpI Bpu1102I BsaBI BsaWI BseAI
BsiWI BsmBI BsmI BspDI BspEI
BspLU11I BsrDI BssHII BstBI CelII
ClaI CpoI DraIII DrdI EagI
EclXI Eco52I EcoNI EcoRV EcoT22I
FbaI FseI FspI HindIII HpaI
KspI MamI MfeI MluI MroI
MunI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmeI PshAI PshBI Psp1406I
PvuI RsrII SacII SalI SbfI
SexAI SfiI SfuI SgrAI SnaBI
Sse8387I SspI StuI SwaI Tth111I
XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AflIII 471 ACACGT A^C[GA][CT]GT
AhdI 730 GACCTCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 621 GTGCAC G^TGCAC
Aor51HI 997 AGCGCT AGC^GCT
ApaLI 621 GTGCAC G^TGCAC
AspEI 730 GACCTCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbeI 773 GGCGCC GGCGC^C
BbrPI 472 CACGTG CAC^GTG
BsaI 175 GAGACC GGTCTC(1/5)
BsiEI 948 CGACCG CG[GA][CT]^CG
Bsp1407I 801 TGTACA T^GTACA
BsrGI 801 TGTACA T^GTACA
BssSI 611 CTCGTG CACGAG(-5/-1)
Bst1107I 714 GTATAC GTA^TAC
BstAPI 1012 GCAGCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 276 GGTGACC G^GT[ACGT]ACC
BstPI 276 GGTGACC G^GT[ACGT]ACC
BstZ17I 714 GTATAC GTA^TAC
DraI 325 TTTAAA TTT^AAA
Eam1105I 730 GACCTCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 781 CTCTTC CTCTTC(1/4)
Eco47III 997 AGCGCT AGC^GCT
EcoO65I 276 GGTGACC G^GT[ACGT]ACC
HgaI 2083 GACGC GACGC(5/10)
HincII 2128 GTCAAC GT[CT]^[GA]AC
HindII 2128 GTCAAC GT[CT]^[GA]AC
KasI 773 GGCGCC G^GCGCC
Ksp632I 781 CTCTTC CTCTTC(1/4)
NarI 773 GGCGCC GG^CGCC
PmaCI 472 CACGTG CAC^GTG
PmlI 472 CACGTG CAC^GTG
PvuII 1013 CAGCTG CAG^CTG
SapI 780 GCTCTTC GCTCTTC(1/4)
ScaI 644 AGTACT AGT^ACT
SfoI 773 GGCGCC GGC^GCC
SmaI 52 CCCGGG CCC^GGG
SpeI 1674 ACTAGT A^CTAGT
SphI 977 GCATGC GCATG^C
SspBI 801 TGTACA T^GTACA
XmaCI 52 CCCGGG C^CCGGG
XmaI 52 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 29 enzymes) #
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Acc65I 677 GGTACC G^GTACC
835 GGTACC
AccI 397 GTCTAC GT^[AC][GT]AC
714 GTATAC
AcsI 385 GAATTC [GA]^AATT[CT]
2245 GAATTC
AcyI 773 GGCGCC G[GA]^CG[CT]C
2083 GACGCC
AocI 297 CCTCAGG CC^T[ACGT]AGG
360 CCTGAGG
ApoI 385 GAATTC [GA]^AATT[CT]
2245 GAATTC
Asp718I 677 GGTACC G^GTACC
835 GGTACC
BbsI 22 GAAGAC GAAGAC(2/6)
2103 GTCTTC
BfaI 1226 CTAG C^TAG
1675 CTAG
BpuAI 22 GAAGAC GAAGAC(2/6)
2103 GTCTTC
BsaAI 400 TACGTG [CT]AC^GT[GA]
472 CACGTG
BsaHI 773 GGCGCC G[GA]^CG[CT]C
2083 GACGCC
BspHI 650 TCATGA T^CATGA
821 TCATGA
Bsu36I 297 CCTCAGG CC^T[ACGT]AGG
360 CCTGAGG
DsaI 1289 CCATGG C^C[GA][CT]GG
2352 CCATGG
Eco81I 297 CCTCAGG CC^T[ACGT]AGG
360 CCTGAGG
EcoRI 385 GAATTC G^AATTC
2245 GAATTC
Hin1I 773 GGCGCC G[GA]^CG[CT]C
2083 GACGCC
KpnI 677 GGTACC GGTAC^C
835 GGTACC
MaeI 1226 CTAG C^TAG
1675 CTAG
NaeI 1466 GCCGGC GCC^GGC
1758 GCCGGC
NcoI 1289 CCATGG C^CATGG
2352 CCATGG
NgoMIV 1466 GCCGGC G^CCGGC
1758 GCCGGC
NspI 515 ACATGC [GA]CATG^[CT]
977 GCATGC
PpuMI 2168 AGGACCC [GA]G^G[AT]CC[CT]
2441 AGGACCC
PstI 1357 CTGCAG CTGCA^G
2383 CTGCAG
RcaI 650 TCATGA T^CATGA
821 TCATGA
SacI 583 GAGCTC GAGCT^C
691 GAGCTC
TfiI 855 GATTC G^A[AT]TC
2305 GATTC
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# 3 OR MORE CUT ENZYMES ( 100 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI ApaI AspHI AvaI AvaII
BalI BanI BanII BbvI BcnI
BglI BmyI BpmI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrFI BsrI BssKI BstF5I BstNI
BstUI BstXI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
EaeI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PflMI PleI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII