(Circular) (Six-base) MAPSORT of: pFN21AB8521.seq Check: 1185 from: 1 to: 7573 pFN21AB8521 7573 bp With 182 enzymes: SgfI * April 15, 2010 09:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4462 4509 4462 Size: 47 7526 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5383 278 Size: 53 83 186 936 3847 2468 Fragments arranged by size: 3847 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4916 1937 Size: 2979 4594 Acc65I G'GTAC_C Cuts at: 3719 3921 4894 3719 Size: 202 973 6398 Fragments arranged by size: 6398 973 202 AclI AA'CG_TT Cuts at: 1563 5700 6073 1563 Size: 4137 373 3063 Fragments arranged by size: 4137 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2439 4013 4127 4429 4631 4707 5636 7020 Size: 807 1574 114 302 202 76 929 1384 Cuts at: 7020 7288 1632 Size: 268 1917 Fragments arranged by size: 1917 1574 1384 929 807 302 268 202 114 76 AfeI AGC'GCT Cuts at: 4034 4034 Size: 7573 AflII C'TTAA_G Cuts at: 820 1017 3146 820 Size: 197 2129 5247 Fragments arranged by size: 5247 2129 197 AflIII A'CryG_T Cuts at: 1203 2160 6472 7449 1203 Size: 957 4312 977 1327 Fragments arranged by size: 4312 1327 977 957 AgeI A'CCGG_T Cuts at: 6404 6404 Size: 7573 AhdI GACnn_n'nnGTC Cuts at: 6302 6302 Size: 7573 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7573 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7573 AlwNI CAG_nnn'CTG Cuts at: 3556 3991 4089 4210 4222 5316 6888 3556 Size: 435 98 121 12 1094 1572 4241 Fragments arranged by size: 4241 1572 1094 435 121 98 12 ApaI G_GGCC'C Cuts at: 3769 4587 3769 Size: 818 6755 ApaLI G'TGCA_C Cuts at: 4463 5630 6786 4463 Size: 1167 1156 5250 Fragments arranged by size: 5250 1167 1156 ApoI r'AATT_y Cuts at: 1493 2413 2975 4882 5108 5260 7369 1493 Size: 920 562 1907 226 152 2109 1697 Fragments arranged by size: 2109 1907 1697 920 562 226 152 AseI AT'TA_AT Cuts at: 160 1032 6127 160 Size: 872 5095 1606 Fragments arranged by size: 5095 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7573 AvaI C'yCGr_G Cuts at: 1870 1943 2644 2891 3650 4898 1870 Size: 73 701 247 759 1248 4545 Fragments arranged by size: 4545 1248 759 701 247 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2169 2202 2911 2944 3703 3736 2169 Size: 33 709 33 759 33 6006 Fragments arranged by size: 6006 759 709 33 33 33 BamHI G'GATC_C Cuts at: 4903 5270 4903 Size: 367 7206 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2029 2036 2713 3719 3921 Size: 325 217 627 242 7 677 1006 202 Cuts at: 3921 4537 4894 6349 618 Size: 616 357 1455 1842 Fragments arranged by size: 1842 1455 1006 677 627 616 357 325 242 217 202 7 BanII G_rGCy'C Cuts at: 1391 2870 3769 4331 4587 4859 4892 1391 Size: 1479 899 562 256 272 33 4072 Fragments arranged by size: 4072 1479 899 562 272 256 33 BbeI G_GCGC'C Cuts at: 2033 2040 2033 Size: 7 7566 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2466 2844 3740 4695 928 Size: 971 567 378 896 955 3806 Fragments arranged by size: 3806 971 955 896 567 378 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 5764 5798 1903 Size: 34 50 34 3743 34 3678 Fragments arranged by size: 3743 3678 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5465 6675 5465 Size: 1210 6363 BclI T'GATC_A Cuts at: 1548 1548 Size: 7573 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2896 3101 3251 Size: 107 122 71 631 605 1224 205 150 Cuts at: 3251 6184 136 Size: 2933 1525 Fragments arranged by size: 2933 1525 1224 631 605 205 150 122 107 71 BglII A'GATC_T Cuts at: 7568 7568 Size: 7573 BlpI GC'TnA_GC Cuts at: 4301 4990 4301 Size: 689 6884 Bme1580I G_kGCm'C Cuts at: 1790 2657 3769 3915 4467 4542 4587 4598 5634 Size: 867 1112 146 552 75 45 11 1036 Cuts at: 5634 6790 1790 Size: 1156 2573 Fragments arranged by size: 2573 1156 1112 1036 867 552 146 75 45 11 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7573 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2475 3724 4871 Size: 416 53 83 399 24 1058 1249 1147 Cuts at: 4871 5304 6262 442 Size: 433 958 1753 Fragments arranged by size: 1753 1249 1147 1058 958 433 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7573 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2030 2039 2416 2541 3269 3485 4160 6233 Size: 791 9 377 125 728 216 675 2073 Cuts at: 6233 1239 Size: 2579 Fragments arranged by size: 2579 2073 791 728 675 377 216 125 9 Bpu10I CC'TnA_GC Cuts at: 2864 4023 2864 Size: 1159 6414 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3032 3197 3281 4382 5698 6563 6861 3032 Size: 165 84 1101 1316 865 298 3744 Fragments arranged by size: 3744 1316 1101 865 298 165 84 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2519 2743 3750 4136 6236 882 Size: 617 1020 224 1007 386 2100 2219 Fragments arranged by size: 2219 2100 1020 1007 617 386 224 BsaAI yAC'GTr Cuts at: 493 1204 3056 4462 5364 493 Size: 711 1852 1406 902 2702 Fragments arranged by size: 2702 1852 1406 902 711 BsaBI GATnn'nnATC Cuts at: 7567 7567 Size: 7573 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2030 2037 3451 4169 Size: 53 83 186 936 497 7 1414 718 Cuts at: 4169 5380 5762 6397 275 Size: 1211 382 635 1451 Fragments arranged by size: 1451 1414 1211 936 718 635 497 382 186 83 53 7 BsaWI w'CCGG_w Cuts at: 3563 4802 6006 6404 6678 6825 7236 3563 Size: 1239 1204 398 274 147 411 3900 Fragments arranged by size: 3900 1239 1204 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2006 2036 3827 3857 1178 Size: 30 165 30 603 30 1791 30 4894 Fragments arranged by size: 4894 1791 603 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2027 2570 2573 2602 3104 3125 3233 3488 Size: 841 543 3 29 502 21 108 255 Cuts at: 3488 3670 3824 3839 3965 4175 4207 4733 4736 Size: 182 154 15 126 210 32 526 3 Cuts at: 4736 4745 4748 1186 Size: 9 3 4011 Fragments arranged by size: 4011 841 543 526 502 255 210 182 154 126 108 32 29 21 15 9 3 3 3 BseYI C'CCAG_C Cuts at: 2687 4215 4361 4586 4808 6776 7272 2687 Size: 1528 146 225 222 1968 496 2988 Fragments arranged by size: 2988 1968 1528 496 225 222 146 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2601 3078 4217 4331 1163 Size: 573 865 477 1139 114 4405 Fragments arranged by size: 4405 1139 865 573 477 114 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5023 5784 6812 1540 Size: 275 186 3022 761 1028 2301 Fragments arranged by size: 3022 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2345 2870 3432 4284 4467 4500 5634 5719 6790 Size: 525 562 852 183 33 1134 85 1071 Cuts at: 6790 2345 Size: 3128 Fragments arranged by size: 3128 1134 1071 852 562 525 183 85 33 BsmI GAATG_Cn' Cuts at: 3595 5084 5177 3595 Size: 1489 93 5991 Fragments arranged by size: 5991 1489 93 BsmBI CGTCTCn'nnnn_ Cuts at: 4191 4191 Size: 7573 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2345 2657 2870 3432 3769 3915 4284 Size: 399 555 312 213 562 337 146 369 Cuts at: 4284 4331 4467 4500 4542 4587 4598 4859 4892 Size: 47 136 33 42 45 11 261 33 Cuts at: 4892 5634 5719 6790 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 562 555 399 369 337 312 261 213 146 136 85 47 45 42 33 33 11 BspEI T'CCGG_A Cuts at: 3563 4802 7236 3563 Size: 1239 2434 3900 Fragments arranged by size: 3900 2434 1239 BspHI T'CATG_A Cuts at: 4650 5462 7192 4650 Size: 812 1730 5031 Fragments arranged by size: 5031 1730 812 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4353 4462 4509 4928 844 Size: 3509 109 47 419 3489 Fragments arranged by size: 3509 3489 419 109 47 BsrBI CCG'CTC Cuts at: 1394 1823 3223 3513 4228 5460 1394 Size: 429 1400 290 715 1232 3507 Fragments arranged by size: 3507 1400 1232 715 429 290 BsrDI GCAATG_nn' Cuts at: 65 1248 4246 6068 6242 65 Size: 1183 2998 1822 174 1396 Fragments arranged by size: 2998 1822 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3389 3546 3702 4150 4313 4832 6217 Size: 110 1579 157 156 448 163 519 1385 Cuts at: 6217 6404 1700 Size: 187 2869 Fragments arranged by size: 2869 1579 1385 519 448 187 163 157 156 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7573 BssHII G'CGCG_C Cuts at: 1925 3750 1925 Size: 1825 5748 BssSI C'ACGA_G Cuts at: 3340 5633 6645 3340 Size: 2293 1012 4268 Fragments arranged by size: 4268 2293 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 4635 794 Size: 3841 3732 BstBI TT'CG_AA Cuts at: 6377 6377 Size: 7573 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7573 BstXI CCAn_nnnn'nTGG Cuts at: 2059 3385 2059 Size: 1326 6247 BstYI r'GATC_y Cuts at: 1972 4206 4903 5270 5655 5672 7124 7210 7222 Size: 2234 697 367 385 17 1452 86 12 Cuts at: 7222 7568 1972 Size: 346 1977 Fragments arranged by size: 2234 1977 1452 697 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3557 3557 Size: 7573 BtgI C'CryG_G Cuts at: 513 1065 2058 2615 3916 4322 4578 513 Size: 552 993 557 1301 406 256 3508 Fragments arranged by size: 3508 1301 993 557 552 406 256 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 4113 487 Size: 683 56 2887 3947 Fragments arranged by size: 3947 2887 683 56 BtsI GCAGTG_nn' Cuts at: 4282 5089 5883 5903 4282 Size: 807 794 20 5952 Fragments arranged by size: 5952 807 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7573 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7538 DraI TTT'AAA Cuts at: 4877 4877 Size: 7573 DraIII CAC_nnn'GTG Cuts at: 4865 4865 Size: 7573 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6580 809 Size: 507 5264 1802 Fragments arranged by size: 5264 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2335 2449 3387 Size: 54 1425 50 275 18 505 114 938 Cuts at: 3387 4672 5020 5909 7538 8 Size: 1285 348 889 1629 43 Fragments arranged by size: 1629 1425 1285 938 889 505 348 275 114 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5020 1537 Size: 275 3208 4090 Fragments arranged by size: 4090 3208 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2881 3129 3279 3300 3816 4607 4722 5503 Size: 1528 248 150 21 516 791 115 781 Cuts at: 5503 1353 Size: 3423 Fragments arranged by size: 3423 1528 791 781 516 248 150 115 21 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3212 3971 6160 6534 6680 387 Size: 1064 138 1623 759 2189 374 146 1280 Fragments arranged by size: 2189 1623 1280 1064 759 374 146 138 EcoICRI GAG'CTC Cuts at: 2868 2868 Size: 7573 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2030 2039 2416 2439 2541 3269 3485 Size: 393 398 9 377 23 102 728 216 Cuts at: 3485 4013 4127 4160 4429 4631 4707 5636 6233 Size: 528 114 33 269 202 76 929 597 Cuts at: 6233 7020 7288 1239 Size: 787 268 1524 Fragments arranged by size: 1524 929 787 728 597 528 398 393 377 269 268 216 202 114 102 76 33 23 9 EcoNI CCTnn'n_nnAGG Cuts at: 3929 4022 3929 Size: 93 7480 EcoO109I rG'GnC_Cy Cuts at: 2291 3373 4583 2291 Size: 1082 1210 5281 Fragments arranged by size: 5281 1210 1082 EcoRI G'AATT_C Cuts at: 4882 5260 4882 Size: 378 7195 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2311 2311 Size: 7573 FspI TGC'GCA Cuts at: 4093 6079 4093 Size: 1986 5587 HaeII r_GCGC'y Cuts at: 1678 2033 2040 2128 4036 6720 1678 Size: 355 7 88 1908 2684 2531 Fragments arranged by size: 2684 2531 1908 355 88 7 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2147 2179 3827 3859 6214 Size: 32 135 32 575 32 1648 32 2355 Cuts at: 6214 6246 6288 6320 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2355 1648 575 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2281 4917 5163 669 Size: 988 281 343 2636 246 3079 Fragments arranged by size: 3079 2636 988 343 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7573 HpaI GTT'AAC Cuts at: 5163 5163 Size: 7573 KasI G'GCGC_C Cuts at: 2029 2036 2029 Size: 7 7566 KpnI G_GTAC'C Cuts at: 3723 3925 4898 3723 Size: 202 973 6398 Fragments arranged by size: 6398 973 202 MfeI C'AATT_G Cuts at: 5172 5172 Size: 7573 MluI A'CGCG_T Cuts at: 7449 7449 Size: 7573 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2657 3434 6687 6871 1305 Size: 1352 777 3253 184 2007 Fragments arranged by size: 3253 2007 1352 777 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2337 2451 10 Size: 54 1425 343 505 114 5132 Fragments arranged by size: 5132 1425 505 343 114 54 MslI CAynn'nnrTG Cuts at: 518 1166 2057 4239 5531 5890 6049 518 Size: 648 891 2182 1292 359 159 2042 Fragments arranged by size: 2182 2042 1292 891 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3208 3487 4219 4324 4625 4989 5666 6814 Size: 1602 279 732 105 301 364 677 1148 Cuts at: 6814 7059 1606 Size: 245 2120 Fragments arranged by size: 2120 1602 1148 732 677 364 301 279 245 105 NaeI GCC'GGC Cuts at: 3391 3548 4152 3391 Size: 157 604 6812 Fragments arranged by size: 6812 604 157 NarI GG'CG_CC Cuts at: 2030 2037 2030 Size: 7 7566 NcoI C'CATG_G Cuts at: 513 1065 2058 3916 513 Size: 552 993 1858 4170 Fragments arranged by size: 4170 1858 993 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7573 NgoMIV G'CCGG_C Cuts at: 3389 3546 4150 3389 Size: 157 604 6812 Fragments arranged by size: 6812 604 157 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7573 NotI GC'GGCC_GC Cuts at: 5020 5020 Size: 7573 NspI r_CATG'y Cuts at: 1132 1229 2164 4636 4931 6476 1132 Size: 97 935 2472 295 1545 2229 Fragments arranged by size: 2472 2229 1545 935 295 97 PasI CC'CwG_GG Cuts at: 1348 3762 1348 Size: 2414 5159 PciI A'CATG_T Cuts at: 2160 6472 2160 Size: 4312 3261 PflMI CCAn_nnn'nTGG Cuts at: 1108 4005 1108 Size: 2897 4676 PfoI T'CCnGG_A Cuts at: 2518 3783 4137 4266 4275 4761 2518 Size: 1265 354 129 9 486 5330 Fragments arranged by size: 5330 1265 486 354 129 9 PmeI GTTT'AAAC Cuts at: 4877 4877 Size: 7573 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5642 5674 7155 7187 5642 Size: 32 1481 32 6028 Fragments arranged by size: 6028 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2291 2291 Size: 7573 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7573 PsiI TTA'TAA Cuts at: 5143 5143 Size: 7573 PspOMI G'GGCC_C Cuts at: 3765 4583 3765 Size: 818 6755 PstI C_TGCA'G Cuts at: 830 3482 3687 4119 4925 830 Size: 2652 205 432 806 3478 Fragments arranged by size: 3478 2652 806 432 205 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7573 PvuII CAG'CTG Cuts at: 3487 4219 3487 Size: 732 6841 RsrII CG'GwC_CG Cuts at: 3456 3456 Size: 7573 SacI G_AGCT'C Cuts at: 2870 2870 Size: 7573 SacII CC_GC'GG Cuts at: 4325 4325 Size: 7573 SalI G'TCGA_C Cuts at: 1936 4915 1936 Size: 2979 4594 SanDI GG'GwC_CC Cuts at: 2291 2291 Size: 7573 SbfI CC_TGCA'GG Cuts at: 4925 4925 Size: 7573 ScaI AGT'ACT Cuts at: 5821 7533 5821 Size: 1712 5861 SfcI C'TryA_G Cuts at: 826 1045 2991 3478 3683 4115 4478 4921 6056 Size: 219 1946 487 205 432 363 443 1135 Cuts at: 6056 6737 6928 826 Size: 681 191 1471 Fragments arranged by size: 1946 1471 1135 681 487 443 432 363 219 205 191 SfiI GGCCn_nnn'nGGCC Cuts at: 3101 3101 Size: 7573 SfoI GGC'GCC Cuts at: 2031 2038 2031 Size: 7 7566 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7573 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7573 SmaI CCC'GGG Cuts at: 1872 4900 1872 Size: 3028 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3047 3146 3212 3260 4397 5677 Size: 197 926 1104 99 66 48 1137 1280 Cuts at: 5677 6578 6840 820 Size: 901 262 1553 Fragments arranged by size: 1553 1280 1137 1104 926 901 262 197 99 66 48 SnaBI TAC'GTA Cuts at: 493 5364 493 Size: 4871 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7573 SphI G_CATG'C Cuts at: 1132 4931 1132 Size: 3799 3774 SspI AAT'ATT Cuts at: 5 52 5497 5 Size: 47 5445 2081 Fragments arranged by size: 5445 2081 47 StuI AGG'CCT Cuts at: 1517 2213 4178 1517 Size: 696 1965 4912 Fragments arranged by size: 4912 1965 696 StyI C'CwwG_G Cuts at: 513 1065 2058 2226 2785 3105 3916 3999 4173 Size: 552 993 168 559 320 811 83 174 Cuts at: 4173 5012 513 Size: 839 3074 Fragments arranged by size: 3074 993 839 811 559 552 320 174 168 83 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2284 4658 4853 5600 5617 5770 5955 Size: 1255 704 2374 195 747 17 153 185 Cuts at: 5955 6400 325 Size: 445 1498 Fragments arranged by size: 2374 1498 1255 747 704 445 195 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2845 5819 7531 Size: 274 80 33 51 1443 868 2974 1712 Cuts at: 7531 96 Size: 138 Fragments arranged by size: 2974 1712 1443 868 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2006 2038 7533 7565 2006 Size: 32 5495 32 2014 Fragments arranged by size: 5495 2014 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7573 XbaI T'CTAG_A Cuts at: 4909 4909 Size: 7573 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4787 4787 Size: 7573 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7573 XmaI C'CCGG_G Cuts at: 1870 4898 1870 Size: 3028 4545 XmnI GAAnn'nnTTC Cuts at: 5702 5702 Size: 7573 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5381 276 Size: 53 83 186 936 3847 2468 Fragments arranged by size: 3847 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BbvCI BfrBI BplI BsiWI BstZ17I EcoRV FseI FspAI NruI NsiI PacI PmlI PspXI PsrI SapI SexAI SrfI SwaI