Restriction map for
pFN21AB8521
(Linear) (Six-base) MAPSORT of: KIBB8521.seq Check: 4990 from: 1 to: 2868
KIBB8521.orf
With 182 enzymes: SgfI *
October 7, 2009 14:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2457 2504 2868
Size: 2457 47 364
Fragments arranged by size:
2457 364 47
Acc65I G'GTAC_C
Cuts at: 0 1714 1916 2868
Size: 1714 202 952
Fragments arranged by size:
1714 952 202
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 434 2008 2122 2424 2626 2702 2868
Size: 434 1574 114 302 202 76 166
Fragments arranged by size:
1574 434 302 202 166 114 76
AfeI AGC'GCT
Cuts at: 0 2029 2868
Size: 2029 839
AflII C'TTAA_G
Cuts at: 0 1141 2868
Size: 1141 1727
AflIII A'CryG_T
Cuts at: 0 155 2868
Size: 155 2713
AlwNI CAG_nnn'CTG
Cuts at: 0 1551 1986 2084 2205 2217 2868
Size: 1551 435 98 121 12 651
Fragments arranged by size:
1551 651 435 121 98 12
ApaI G_GGCC'C
Cuts at: 0 1764 2582 2868
Size: 1764 818 286
Fragments arranged by size:
1764 818 286
ApaLI G'TGCA_C
Cuts at: 0 2458 2868
Size: 2458 410
ApoI r'AATT_y
Cuts at: 0 408 970 2868
Size: 408 562 1898
Fragments arranged by size:
1898 562 408
AvaI C'yCGr_G
Cuts at: 0 639 886 1645 2868
Size: 639 247 759 1223
Fragments arranged by size:
1223 759 639 247
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 164 197 906 939 1698 1731 2868
Size: 164 33 709 33 759 33 1137
Fragments arranged by size:
1137 759 709 164 33 33 33
BanI G'GyrC_C
Cuts at: 0 24 31 708 1714 1916 2532 2868
Size: 24 7 677 1006 202 616 336
Fragments arranged by size:
1006 677 616 336 202 24 7
BanII G_rGCy'C
Cuts at: 0 865 1764 2326 2582 2854 2868
Size: 865 899 562 256 272 14
Fragments arranged by size:
899 865 562 272 256 14
BbeI G_GCGC'C
Cuts at: 0 28 35 2868
Size: 28 7 2833
Fragments arranged by size:
2833 28 7
BbsI GAAGACnn'nnnn_
Cuts at: 0 461 839 1735 2690 2868
Size: 461 378 896 955 178
Fragments arranged by size:
955 896 461 378 178
BglI GCCn_nnn'nGGC
Cuts at: 0 891 1096 1246 2868
Size: 891 205 150 1622
Fragments arranged by size:
1622 891 205 150
BlpI GC'TnA_GC
Cuts at: 0 2296 2868
Size: 2296 572
Bme1580I G_kGCm'C
Cuts at: 0 652 1764 1910 2462 2537 2582 2593 2868
Size: 652 1112 146 552 75 45 11 275
Fragments arranged by size:
1112 652 552 275 146 75 45 11
BmrI ACTGGGnnnn_n'
Cuts at: 0 470 1719 2866 2868
Size: 470 1249 1147 2
Fragments arranged by size:
1249 1147 470 2
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 25 34 411 536 1264 1480 2155 2868
Size: 25 9 377 125 728 216 675 713
Fragments arranged by size:
728 713 675 377 216 125 25 9
Bpu10I CC'TnA_GC
Cuts at: 0 859 2018 2868
Size: 859 1159 850
Fragments arranged by size:
1159 859 850
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1027 1192 1276 2377 2868
Size: 1027 165 84 1101 491
Fragments arranged by size:
1101 1027 491 165 84
BsaI GGTCTCn'nnnn_
Cuts at: 0 514 738 1745 2131 2868
Size: 514 224 1007 386 737
Fragments arranged by size:
1007 737 514 386 224
BsaAI yAC'GTr
Cuts at: 0 1051 2457 2868
Size: 1051 1406 411
Fragments arranged by size:
1406 1051 411
BsaHI Gr'CG_yC
Cuts at: 0 25 32 1446 2164 2868
Size: 25 7 1414 718 704
Fragments arranged by size:
1414 718 704 25 7
BsaWI w'CCGG_w
Cuts at: 0 1558 2797 2868
Size: 1558 1239 71
Fragments arranged by size:
1558 1239 71
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1 31 1822 1852 2868
Size: 1 30 1791 30 1016
Fragments arranged by size:
1791 1016 30 30 1
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 22 565 568 597 1099 1120 1228 1483
Size: 22 543 3 29 502 21 108 255
Cuts at: 1483 1665 1819 1834 1960 2170 2202 2728 2731
Size: 182 154 15 126 210 32 526 3
Cuts at: 2731 2740 2743 2868
Size: 9 3 125
Fragments arranged by size:
543 526 502 255 210 182 154 126
125 108 32 29 22 21 15 9
3 3 3
BseYI C'CCAG_C
Cuts at: 0 682 2210 2356 2581 2803 2868
Size: 682 1528 146 225 222 65
Fragments arranged by size:
1528 682 225 222 146 65
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 596 1073 2212 2326 2868
Size: 596 477 1139 114 542
Fragments arranged by size:
1139 596 542 477 114
BsiHKAI G_wGCw'C
Cuts at: 0 340 865 1427 2279 2462 2495 2868
Size: 340 525 562 852 183 33 373
Fragments arranged by size:
852 562 525 373 340 183 33
BsmI GAATG_Cn'
Cuts at: 0 1590 2868
Size: 1590 1278
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2186 2868
Size: 2186 682
Bsp1286I G_dGCh'C
Cuts at: 0 340 652 865 1427 1764 1910 2279 2326
Size: 340 312 213 562 337 146 369 47
Cuts at: 2326 2462 2495 2537 2582 2593 2854 2868
Size: 136 33 42 45 11 261 14
Fragments arranged by size:
562 369 340 337 312 261 213 146
136 47 45 42 33 14 11
BspEI T'CCGG_A
Cuts at: 0 1558 2797 2868
Size: 1558 1239 71
Fragments arranged by size:
1558 1239 71
BspHI T'CATG_A
Cuts at: 0 2645 2868
Size: 2645 223
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2348 2457 2504 2868
Size: 2348 109 47 364
Fragments arranged by size:
2348 364 109 47
BsrBI CCG'CTC
Cuts at: 0 1218 1508 2223 2868
Size: 1218 290 715 645
Fragments arranged by size:
1218 715 645 290
BsrDI GCAATG_nn'
Cuts at: 0 2241 2868
Size: 2241 627
BsrFI r'CCGG_y
Cuts at: 0 1384 1541 1697 2145 2308 2827 2868
Size: 1384 157 156 448 163 519 41
Fragments arranged by size:
1384 519 448 163 157 156 41
BssHII G'CGCG_C
Cuts at: 0 1745 2868
Size: 1745 1123
BssSI C'ACGA_G
Cuts at: 0 1335 2868
Size: 1335 1533
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2630 2868
Size: 2630 238
BstXI CCAn_nnnn'nTGG
Cuts at: 0 54 1380 2868
Size: 54 1326 1488
Fragments arranged by size:
1488 1326 54
BstYI r'GATC_y
Cuts at: 0 2201 2868
Size: 2201 667
Bsu36I CC'TnA_GG
Cuts at: 0 1552 2868
Size: 1552 1316
BtgI C'CryG_G
Cuts at: 0 53 610 1911 2317 2573 2868
Size: 53 557 1301 406 256 295
Fragments arranged by size:
1301 557 406 295 256 53
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2108 2868
Size: 2108 760
BtsI GCAGTG_nn'
Cuts at: 0 2277 2868
Size: 2277 591
DraIII CAC_nnn'GTG
Cuts at: 0 2860 2868
Size: 2860 8
EaeI y'GGCC_r
Cuts at: 0 330 444 1382 2667 2868
Size: 330 114 938 1285 201
Fragments arranged by size:
1285 938 330 201 114
EarI CTCTTCn'nnn_
Cuts at: 0 876 1124 1274 1295 1811 2602 2717 2868
Size: 876 248 150 21 516 791 115 151
Fragments arranged by size:
876 791 516 248 151 150 115 21
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1207 1966 2868
Size: 1207 759 902
Fragments arranged by size:
1207 902 759
EcoICRI GAG'CTC
Cuts at: 0 863 2868
Size: 863 2005
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 25 34 411 434 536 1264 1480 2008
Size: 25 9 377 23 102 728 216 528
Cuts at: 2008 2122 2155 2424 2626 2702 2868
Size: 114 33 269 202 76 166
Fragments arranged by size:
728 528 377 269 216 202 166 114
102 76 33 25 23 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1924 2017 2868
Size: 1924 93 851
Fragments arranged by size:
1924 851 93
EcoO109I rG'GnC_Cy
Cuts at: 0 286 1368 2578 2868
Size: 286 1082 1210 290
Fragments arranged by size:
1210 1082 290 286
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 306 2868
Size: 306 2562
FspI TGC'GCA
Cuts at: 0 2088 2868
Size: 2088 780
HaeII r_GCGC'y
Cuts at: 0 28 35 123 2031 2868
Size: 28 7 88 1908 837
Fragments arranged by size:
1908 837 88 28 7
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 142 174 1822 1854 2868
Size: 142 32 1648 32 1014
Fragments arranged by size:
1648 1014 142 32 32
HincII GTy'rAC
Cuts at: 0 276 2868
Size: 276 2592
KasI G'GCGC_C
Cuts at: 0 24 31 2868
Size: 24 7 2837
Fragments arranged by size:
2837 24 7
KpnI G_GTAC'C
Cuts at: 0 1718 1920 2868
Size: 1718 202 948
Fragments arranged by size:
1718 948 202
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 652 1429 2868
Size: 652 777 1439
Fragments arranged by size:
1439 777 652
MscI TGG'CCA
Cuts at: 0 332 446 2868
Size: 332 114 2422
Fragments arranged by size:
2422 332 114
MslI CAynn'nnrTG
Cuts at: 0 52 2234 2868
Size: 52 2182 634
Fragments arranged by size:
2182 634 52
MspA1I CmG'CkG
Cuts at: 0 1203 1482 2214 2319 2620 2868
Size: 1203 279 732 105 301 248
Fragments arranged by size:
1203 732 301 279 248 105
NaeI GCC'GGC
Cuts at: 0 1386 1543 2147 2868
Size: 1386 157 604 721
Fragments arranged by size:
1386 721 604 157
NarI GG'CG_CC
Cuts at: 0 25 32 2868
Size: 25 7 2836
Fragments arranged by size:
2836 25 7
NcoI C'CATG_G
Cuts at: 0 53 1911 2868
Size: 53 1858 957
Fragments arranged by size:
1858 957 53
NgoMIV G'CCGG_C
Cuts at: 0 1384 1541 2145 2868
Size: 1384 157 604 723
Fragments arranged by size:
1384 723 604 157
NspI r_CATG'y
Cuts at: 0 159 2631 2868
Size: 159 2472 237
Fragments arranged by size:
2472 237 159
PasI CC'CwG_GG
Cuts at: 0 1757 2868
Size: 1757 1111
PciI A'CATG_T
Cuts at: 0 155 2868
Size: 155 2713
PflMI CCAn_nnn'nTGG
Cuts at: 0 2000 2868
Size: 2000 868
PfoI T'CCnGG_A
Cuts at: 0 513 1778 2132 2261 2270 2756 2868
Size: 513 1265 354 129 9 486 112
Fragments arranged by size:
1265 513 486 354 129 112 9
PpuMI rG'GwC_Cy
Cuts at: 0 286 2868
Size: 286 2582
PspOMI G'GGCC_C
Cuts at: 0 1760 2578 2868
Size: 1760 818 290
Fragments arranged by size:
1760 818 290
PstI C_TGCA'G
Cuts at: 0 1477 1682 2114 2868
Size: 1477 205 432 754
Fragments arranged by size:
1477 754 432 205
PvuII CAG'CTG
Cuts at: 0 1482 2214 2868
Size: 1482 732 654
Fragments arranged by size:
1482 732 654
RsrII CG'GwC_CG
Cuts at: 0 1451 2868
Size: 1451 1417
SacI G_AGCT'C
Cuts at: 0 865 2868
Size: 865 2003
SacII CC_GC'GG
Cuts at: 0 2320 2868
Size: 2320 548
SanDI GG'GwC_CC
Cuts at: 0 286 2868
Size: 286 2582
SfcI C'TryA_G
Cuts at: 0 986 1473 1678 2110 2473 2868
Size: 986 487 205 432 363 395
Fragments arranged by size:
986 487 432 395 363 205
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1096 2868
Size: 1096 1772
SfoI GGC'GCC
Cuts at: 0 26 33 2868
Size: 26 7 2835
Fragments arranged by size:
2835 26 7
SmlI C'TyrA_G
Cuts at: 0 1042 1141 1207 1255 2392 2868
Size: 1042 99 66 48 1137 476
Fragments arranged by size:
1137 1042 476 99 66 48
StuI AGG'CCT
Cuts at: 0 208 2173 2868
Size: 208 1965 695
Fragments arranged by size:
1965 695 208
StyI C'CwwG_G
Cuts at: 0 53 221 780 1100 1911 1994 2168 2868
Size: 53 168 559 320 811 83 174 700
Fragments arranged by size:
811 700 559 320 174 168 83 53
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 279 2653 2848 2868
Size: 279 2374 195 20
Fragments arranged by size:
2374 279 195 20
TatI w'GTAC_w
Cuts at: 0 840 2868
Size: 840 2028
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1 33 2868
Size: 1 32 2835
Fragments arranged by size:
2835 32 1
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2782 2868
Size: 2782 86
Enzymes that do cut:
AarI Acc65I AcuI AfeI AflII AflIII AlwNI ApaI
ApaLI ApoI AvaI BaeI BanI BanII BbeI BbsI
BglI BlpI Bme1580I BmrI BpmI Bpu10I BpuEI BsaI
BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssHII BssSI BstAPI BstXI BstYI Bsu36I BtgI
BtgZI BtsI DraIII EaeI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I FalI FspI HaeII Hin4I HincII KasI
KpnI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PasI PciI PflMI PfoI PpuMI PspOMI
PstI PvuII RsrII SacI SacII SanDI SfcI SfiI
SfoI SmlI StuI StyI TaqII TatI TstI XcmI
Enzymes that do not cut:
AatII AccI AclI AgeI AhdI AleI AlfI AloI
AscI AseI AsiSI AvrII BamHI BbvCI BcgI BciVI
BclI BfrBI BglII BmgBI BmtI BplI BsaBI BsiEI
BsiWI BsrGI BstBI BstEII BstZ17I ClaI CspCI DraI
DrdI EagI EcoRI EcoRV FseI FspAI HindIII HpaI
MfeI MluI NdeI NheI NotI NruI NsiI PacI
PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI
SalI SapI SbfI ScaI SexAI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI SwaI Tth111I XbaI
XhoI XmaI XmnI ZraI