Restriction map for
pFN21AB5718
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# NO CUT ENZYMES ( 123 enzymes) #
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AatII Acc65I AccI AccII AclI
AcyI AflII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AspHI
AspI AviII AvrII BalI BanI
BanII BbeI BbvCI BfrI BglI
BlnI BlpI Bpu1102I BsaBI BsaHI
BsaI BsgI BsiEI BsiHKAI BsiWI
BsmBI Bsp1407I BspDI BspLU11I BsrFI
BsrGI BssHII Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DraII DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO109I
EcoO65I EcoRI FauI FseI FspI
HgaI Hin1I HindIII HpaI KasI
KpnI KspI MamI MfeI MluI
MluNI MscI MunI MvnI NaeI
NarI NgoMIV NheI NotI NruI
NspV PacI PflFI PinAI PmeI
PpuMI PshAI PshBI Psp1406I PstI
PvuI RsrII SacI SacII SalI
SapI SbfI SfiI SfoI SfuI
SgrAI SnaBI SpeI Sse8387I SspBI
StuI Tth111I XbaI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AccIII 25 TCCGGA T^CCGGA
AflIII 1075 ACGTGT A^C[GA][CT]GT
AvaII 1493 GGTCC G^G[AT]CC
BamHI 534 GGATCC G^GATCC
BbrPI 740 CACGTG CAC^GTG
BcgI 1132 GCAGGGATGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BmyI 743 GTGCCC G[AGT]GC[ACT]^C
BseAI 25 TCCGGA T^CCGGA
Bsp1286I 743 GTGCCC G[AGT]GC[ACT]^C
BspEI 25 TCCGGA T^CCGGA
BspHI 1053 TCATGA T^CATGA
BspMI 779 ACCTGC ACCTGC(4/8)
BsrBI 3 GAGCGG CCGCTC(-3/-3)
BsrDI 638 GCAATG GCAATG(2/0)
BssSI 1226 CACGAG CACGAG(-5/-1)
CfoI 1684 GCGC GCG^C
Cfr13I 1493 GGTCC G^G[ACGT]CC
EcoNI 1126 CCTACAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 256 GATATC GAT^ATC
EcoT22I 71 ATGCAT ATGCA^T
HaeII 1683 AGCGCC [GA]GCGC^[CT]
HhaI 1684 GCGC GCG^C
HinP1I 1684 GCGC G^CGC
MroI 25 TCCGGA T^CCGGA
MslI 1837 CACGACAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 404 CAGCTG C[AC]G^C[GT]G
NcoI 1617 CCATGG C^CATGG
NdeI 1480 CATATG CA^TATG
NsiI 71 ATGCAT ATGCA^T
PaeR7I 1763 CTCGAG C^TCGAG
PflMI 931 CCAACACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 740 CACGTG CAC^GTG
PmlI 740 CACGTG CAC^GTG
PvuII 404 CAGCTG CAG^CTG
RcaI 1053 TCATGA T^CATGA
Sau96I 1493 GGTCC G^G[ACGT]CC
ScaI 829 AGTACT AGT^ACT
SexAI 936 ACCTGGT A^CC[AT]GGT
SfcI 1127 CTACAG C^T[GA][CT]AG
SmaI 2108 CCCGGG CCC^GGG
SphI 597 GCATGC GCATG^C
SspI 137 AATATT AAT^ATT
SwaI 182 ATTTAAAT ATTT^AAAT
Van91I 931 CCAACACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 1763 CTCGAG C^TCGAG
XmaCI 2108 CCCGGG C^CCGGG
XmaI 2108 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 19 enzymes) #
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AcsI 186 AAATTT [GA]^AATT[CT]
1598 AAATTT
AfaI 830 GTAC GT^AC
2105 GTAC
AlwNI 1100 CAGACACTG CAG[ACGT][ACGT][ACGT]^CTG
1523 CAGTTCCTG
ApoI 186 AAATTT [GA]^AATT[CT]
1598 AAATTT
AvaI 1763 CTCGAG C^[CT]CG[GA]G
2108 CCCGGG
BbsI 1885 GAAGAC GAAGAC(2/6)
2085 GAAGAC
BciVI 1082 GTATCC GTATCC(6/5)
1121 GTATCC
BclI 150 TGATCA T^GATCA
1937 TGATCA
BpuAI 1885 GAAGAC GAAGAC(2/6)
2085 GAAGAC
BsaWI 25 TCCGGA [AT]^CCGG[AT]
272 ACCGGA
BsmI 1457 GAATGC GAATGC(1/-1)
524 GCATTC
BsoBI 1763 CTCGAG C^[CT]CG[GA]G
2108 CCCGGG
DsaI 1553 CCGTGG C^C[GA][CT]GG
1617 CCATGG
EaeI 105 TGGCCG [CT]^GGCC[GA]
1030 CGGCCA
FbaI 150 TGATCA T^GATCA
1937 TGATCA
NspI 597 GCATGC [GA]CATG^[CT]
903 GCATGT
RsaI 830 GTAC GT^AC
2105 GTAC
SfaNI 205 GATGC GCATC(5/9)
1195 GATGC
XcmI 678 CCAGAGCAAACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1160 CCATTTCCACTGTGG
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# 3 OR MORE CUT ENZYMES ( 69 enzymes) #
#########################################
AciI AluI AlwI Asp700I BbvI
BcnI BfaI BglII BpmI BsaAI
BsaJI BseRI BsiYI BslI BsmAI
BsmFI BsrI BssKI BstF5I BstNI
BstYI Cac8I DdeI DpnII DraI
EarI EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PleI
Sau3AI ScrFI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII XmnI