Restriction map for
pFN21AB5718
(Linear) (Six-base) MAPSORT of: KIBB5718.seq Check: 8181 from: 1 to: 2172
KIBB5718.orf
With 182 enzymes: SgfI *
October 7, 2009 14:17 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 740 854 1473 2172
Size: 740 114 619 699
Fragments arranged by size:
740 699 619 114
AflIII A'CryG_T
Cuts at: 0 1075 2172
Size: 1075 1097
AleI CACnn'nnGTG
Cuts at: 0 1841 2172
Size: 1841 331
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1448 1480 2172
Size: 1448 32 692
Fragments arranged by size:
1448 692 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1105 1528 2172
Size: 1105 423 644
Fragments arranged by size:
1105 644 423
ApoI r'AATT_y
Cuts at: 0 186 1598 2172
Size: 186 1412 574
Fragments arranged by size:
1412 574 186
AvaI C'yCGr_G
Cuts at: 0 1763 2108 2172
Size: 1763 345 64
Fragments arranged by size:
1763 345 64
BamHI G'GATC_C
Cuts at: 0 534 2172
Size: 534 1638
BbsI GAAGACnn'nnnn_
Cuts at: 0 1892 2092 2172
Size: 1892 200 80
Fragments arranged by size:
1892 200 80
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1121 1155 2172
Size: 1121 34 1017
Fragments arranged by size:
1121 1017 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1093 1132 2172
Size: 1093 39 1040
Fragments arranged by size:
1093 1040 39
BclI T'GATC_A
Cuts at: 0 150 1937 2172
Size: 150 1787 235
Fragments arranged by size:
1787 235 150
BfrBI ATG'CAT
Cuts at: 0 73 2172
Size: 73 2099
BglII A'GATC_T
Cuts at: 0 1246 1738 1974 2172
Size: 1246 492 236 198
Fragments arranged by size:
1246 492 236 198
Bme1580I G_kGCm'C
Cuts at: 0 747 2172
Size: 747 1425
BmrI ACTGGGnnnn_n'
Cuts at: 0 1726 2172
Size: 1726 446
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1778 2172
Size: 1778 394
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 137 750 1412 2172
Size: 137 613 662 760
Fragments arranged by size:
760 662 613 137
Bpu10I CC'TnA_GC
Cuts at: 0 2031 2172
Size: 2031 141
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 524 1190 1552 2172
Size: 524 666 362 620
Fragments arranged by size:
666 620 524 362
BsaAI yAC'GTr
Cuts at: 0 742 1150 2011 2172
Size: 742 408 861 161
Fragments arranged by size:
861 742 408 161
BsaWI w'CCGG_w
Cuts at: 0 25 272 2172
Size: 25 247 1900
Fragments arranged by size:
1900 247 25
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 43 73 459 489 2172
Size: 43 30 386 30 1683
Fragments arranged by size:
1683 386 43 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 96 316 319 467 1460 2172
Size: 96 220 3 148 993 712
Fragments arranged by size:
993 712 220 148 96 3
BsmI GAATG_Cn'
Cuts at: 0 524 1463 2172
Size: 524 939 709
Fragments arranged by size:
939 709 524
Bsp1286I G_dGCh'C
Cuts at: 0 747 2172
Size: 747 1425
BspEI T'CCGG_A
Cuts at: 0 25 2172
Size: 25 2147
BspHI T'CATG_A
Cuts at: 0 1053 2172
Size: 1053 1119
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 788 2172
Size: 788 1384
BsrBI CCG'CTC
Cuts at: 0 5 2172
Size: 5 2167
BsrDI GCAATG_nn'
Cuts at: 0 645 2172
Size: 645 1527
BssSI C'ACGA_G
Cuts at: 0 1226 2172
Size: 1226 946
BstYI r'GATC_y
Cuts at: 0 120 366 534 1246 1738 1974 2172
Size: 120 246 168 712 492 236 198
Fragments arranged by size:
712 492 246 236 198 168 120
BtgI C'CryG_G
Cuts at: 0 1553 1617 2172
Size: 1553 64 555
Fragments arranged by size:
1553 555 64
BtsI GCAGTG_nn'
Cuts at: 0 108 1833 2031 2046 2172
Size: 108 1725 198 15 126
Fragments arranged by size:
1725 198 126 108 15
DraI TTT'AAA
Cuts at: 0 185 1178 1281 2172
Size: 185 993 103 891
Fragments arranged by size:
993 891 185 103
EaeI y'GGCC_r
Cuts at: 0 105 1030 2172
Size: 105 925 1142
Fragments arranged by size:
1142 925 105
EarI CTCTTCn'nnn_
Cuts at: 0 73 627 1776 1841 2155 2172
Size: 73 554 1149 65 314 17
Fragments arranged by size:
1149 554 314 73 65 17
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 137 740 750 854 1412 1473 2172
Size: 137 603 10 104 558 61 699
Fragments arranged by size:
699 603 558 137 104 61 10
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1130 2172
Size: 1130 1042
EcoRV GAT'ATC
Cuts at: 0 258 2172
Size: 258 1914
HaeII r_GCGC'y
Cuts at: 0 1687 2172
Size: 1687 485
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 445 477 1432 1464 1730 1762 2079 2111
Size: 445 32 955 32 266 32 317 32
Cuts at: 2111 2172
Size: 61
Fragments arranged by size:
955 445 317 266 61 32 32 32
32
HincII GTy'rAC
Cuts at: 0 196 762 1298 2076 2172
Size: 196 566 536 778 96
Fragments arranged by size:
778 566 536 196 96
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 379 955 2172
Size: 379 576 1217
Fragments arranged by size:
1217 576 379
MslI CAynn'nnrTG
Cuts at: 0 1841 2172
Size: 1841 331
MspA1I CmG'CkG
Cuts at: 0 406 2172
Size: 406 1766
NcoI C'CATG_G
Cuts at: 0 1617 2172
Size: 1617 555
NdeI CA'TA_TG
Cuts at: 0 1481 2172
Size: 1481 691
NsiI A_TGCA'T
Cuts at: 0 75 2172
Size: 75 2097
NspI r_CATG'y
Cuts at: 0 601 907 2172
Size: 601 306 1265
Fragments arranged by size:
1265 601 306
PflMI CCAn_nnn'nTGG
Cuts at: 0 937 2172
Size: 937 1235
PfoI T'CCnGG_A
Cuts at: 0 1428 2172
Size: 1428 744
PmlI CAC'GTG
Cuts at: 0 742 2172
Size: 742 1430
PsiI TTA'TAA
Cuts at: 0 776 2172
Size: 776 1396
PvuII CAG'CTG
Cuts at: 0 406 2172
Size: 406 1766
ScaI AGT'ACT
Cuts at: 0 831 2172
Size: 831 1341
SexAI A'CCwGG_T
Cuts at: 0 936 2172
Size: 936 1236
SfcI C'TryA_G
Cuts at: 0 1127 2172
Size: 1127 1045
SmaI CCC'GGG
Cuts at: 0 2110 2172
Size: 2110 62
SmlI C'TyrA_G
Cuts at: 0 539 1205 1567 1763 2172
Size: 539 666 362 196 409
Fragments arranged by size:
666 539 409 362 196
SphI G_CATG'C
Cuts at: 0 601 2172
Size: 601 1571
SspI AAT'ATT
Cuts at: 0 139 2172
Size: 139 2033
StyI C'CwwG_G
Cuts at: 0 980 1617 2090 2172
Size: 980 637 473 82
Fragments arranged by size:
980 637 473 82
SwaI ATTT'AAAT
Cuts at: 0 185 2172
Size: 185 1987
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 460 1073 2172
Size: 460 613 1099
Fragments arranged by size:
1099 613 460
TatI w'GTAC_w
Cuts at: 0 829 2172
Size: 829 1343
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 867 899 2172
Size: 867 32 1273
Fragments arranged by size:
1273 867 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 685 1167 2172
Size: 685 482 1005
Fragments arranged by size:
1005 685 482
XhoI C'TCGA_G
Cuts at: 0 1763 2172
Size: 1763 409
XmaI C'CCGG_G
Cuts at: 0 2108 2172
Size: 2108 64
XmnI GAAnn'nnTTC
Cuts at: 0 1311 1397 1635 2172
Size: 1311 86 238 537
Fragments arranged by size:
1311 537 238 86
Enzymes that do cut:
AcuI AflIII AleI AloI AlwNI ApoI AvaI BamHI
BbsI BcgI BciVI BclI BfrBI BglII Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaAI BsaWI BsaXI BseRI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BssSI
BstYI BtgI BtsI DraI EaeI EarI Eco57MI EcoNI
EcoRV HaeII Hin4I HincII MmeI MslI MspA1I NcoI
NdeI NsiI NspI PflMI PfoI PmlI PsiI PvuII
ScaI SexAI SfcI SmaI SmlI SphI SspI StyI
SwaI TaqII TatI TstI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AlfI ApaI ApaLI AscI AseI AsiSI AvrII
BaeI BanI BanII BbeI BbvCI BglI BlpI BmgBI
BmtI BsaI BsaBI BsaHI BseYI BsgI BsiEI BsiHKAI
BsiWI BsmBI BsrFI BsrGI BssHII BstAPI BstBI BstEII
BstXI BstZ17I Bsu36I BtgZI ClaI CspCI DraIII DrdI
EagI EciI EcoICRI EcoO109I EcoRI FalI FseI FspI
FspAI HindIII HpaI KasI KpnI MfeI MluI MscI
NaeI NarI NgoMIV NheI NotI NruI PacI PasI
PciI PmeI PpiI PpuMI PshAI PspOMI PspXI PsrI
PstI PvuI RsrII SacI SacII SalI SanDI SapI
SbfI SfiI SfoI SgfI SgrAI SnaBI SpeI SrfI
StuI Tth111I XbaI ZraI