Restriction map for
pFN21AB5710
(Linear) (Six-base) MAPSORT of: KIBB5710.seq Check: 3339 from: 1 to: 2151
KIBB5710.orf
With 182 enzymes: SgfI *
October 7, 2009 14:16 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 978 1092 2151
Size: 978 114 1059
Fragments arranged by size:
1059 978 114
Acc65I G'GTAC_C
Cuts at: 0 685 2151
Size: 685 1466
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 220 235 328 695 741 898 1375 1613
Size: 220 15 93 367 46 157 477 238
Cuts at: 1613 2151
Size: 538
Fragments arranged by size:
538 477 367 238 220 157 93 46
15
AfeI AGC'GCT
Cuts at: 0 577 2151
Size: 577 1574
AhdI GACnn_n'nnGTC
Cuts at: 0 483 516 2151
Size: 483 33 1635
Fragments arranged by size:
1635 483 33
AleI CACnn'nnGTG
Cuts at: 0 1330 2151
Size: 1330 821
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 659 1840 2029 2151
Size: 659 1181 189 122
Fragments arranged by size:
1181 659 189 122
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1895 1927 2151
Size: 1895 32 224
Fragments arranged by size:
1895 224 32
AlwNI CAG_nnn'CTG
Cuts at: 0 90 630 846 876 1113 1991 2079 2151
Size: 90 540 216 30 237 878 88 72
Fragments arranged by size:
878 540 237 216 90 88 72 30
ApaI G_GGCC'C
Cuts at: 0 1545 2151
Size: 1545 606
BamHI G'GATC_C
Cuts at: 0 1314 2151
Size: 1314 837
BanI G'GyrC_C
Cuts at: 0 685 953 1863 2040 2151
Size: 685 268 910 177 111
Fragments arranged by size:
910 685 268 177 111
BanII G_rGCy'C
Cuts at: 0 332 458 1069 1370 1545 2151
Size: 332 126 611 301 175 606
Fragments arranged by size:
611 606 332 301 175 126
BbeI G_GCGC'C
Cuts at: 0 957 2044 2151
Size: 957 1087 107
Fragments arranged by size:
1087 957 107
BbsI GAAGACnn'nnnn_
Cuts at: 0 899 2151
Size: 899 1252
BbvCI CC'TCA_GC
Cuts at: 0 551 970 2151
Size: 551 419 1181
Fragments arranged by size:
1181 551 419
BglI GCCn_nnn'nGGC
Cuts at: 0 865 962 1064 1144 2151
Size: 865 97 102 80 1007
Fragments arranged by size:
1007 865 102 97 80
BglII A'GATC_T
Cuts at: 0 1653 2151
Size: 1653 498
BlpI GC'TnA_GC
Cuts at: 0 17 2151
Size: 17 2134
Bme1580I G_kGCm'C
Cuts at: 0 1545 1866 2151
Size: 1545 321 285
Fragments arranged by size:
1545 321 285
BmrI ACTGGGnnnn_n'
Cuts at: 0 2049 2151
Size: 2049 102
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1251 2151
Size: 1251 900
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 145 610 754 784 826 949 1021 1063
Size: 145 465 144 30 42 123 72 42
Cuts at: 1063 1105 1198 1417 1486 1642 1678 1714 1853
Size: 42 93 219 69 156 36 36 139
Cuts at: 1853 1948 2151
Size: 95 203
Fragments arranged by size:
465 219 203 156 145 144 139 123
95 93 72 69 42 42 42 36
36 30
Bpu10I CC'TnA_GC
Cuts at: 0 91 551 970 1369 2151
Size: 91 460 419 399 782
Fragments arranged by size:
782 460 419 399 91
BsaI GGTCTCn'nnnn_
Cuts at: 0 35 700 2151
Size: 35 665 1451
Fragments arranged by size:
1451 665 35
BsaAI yAC'GTr
Cuts at: 0 1803 2151
Size: 1803 348
BsaHI Gr'CG_yC
Cuts at: 0 954 2041 2112 2151
Size: 954 1087 71 39
Fragments arranged by size:
1087 954 71 39
BsaWI w'CCGG_w
Cuts at: 0 833 2151
Size: 833 1318
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1171 1201 1204 1234 2151
Size: 1171 30 3 30 917
Fragments arranged by size:
1171 917 30 30 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 559 568 631 763 1147 1270 1462 1702
Size: 559 9 63 132 384 123 192 240
Cuts at: 1702 1747 1798 2047 2151
Size: 45 51 249 104
Fragments arranged by size:
559 384 249 240 192 132 123 104
63 51 45 9
BseYI C'CCAG_C
Cuts at: 0 354 728 919 1079 1271 2026 2151
Size: 354 374 191 160 192 755 125
Fragments arranged by size:
755 374 354 192 191 160 125
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1126 1434 2151
Size: 1126 308 717
Fragments arranged by size:
1126 717 308
BsiEI CG_ry'CG
Cuts at: 0 1430 1434 2151
Size: 1430 4 717
Fragments arranged by size:
1430 717 4
BsiHKAI G_wGCw'C
Cuts at: 0 332 458 1370 2151
Size: 332 126 912 781
Fragments arranged by size:
912 781 332 126
BsmI GAATG_Cn'
Cuts at: 0 648 1171 2151
Size: 648 523 980
Fragments arranged by size:
980 648 523
BsmBI CGTCTCn'nnnn_
Cuts at: 0 71 487 1369 1492 2151
Size: 71 416 882 123 659
Fragments arranged by size:
882 659 416 123 71
Bsp1286I G_dGCh'C
Cuts at: 0 332 458 1069 1370 1545 1866 2151
Size: 332 126 611 301 175 321 285
Fragments arranged by size:
611 332 321 301 285 175 126
BspHI T'CATG_A
Cuts at: 0 404 458 1644 2151
Size: 404 54 1186 507
Fragments arranged by size:
1186 507 404 54
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 515 978 1092 1565 2151
Size: 515 463 114 473 586
Fragments arranged by size:
586 515 473 463 114
BsrBI CCG'CTC
Cuts at: 0 594 660 1063 1215 1230 1440 2037 2151
Size: 594 66 403 152 15 210 597 114
Fragments arranged by size:
597 594 403 210 152 114 66 15
BsrFI r'CCGG_y
Cuts at: 0 668 950 1015 1508 2151
Size: 668 282 65 493 643
Fragments arranged by size:
668 643 493 282 65
BssHII G'CGCG_C
Cuts at: 0 1074 1481 1483 2151
Size: 1074 407 2 668
Fragments arranged by size:
1074 668 407 2
BstAPI GCAn_nnn'nTGC
Cuts at: 0 603 1113 1749 2151
Size: 603 510 636 402
Fragments arranged by size:
636 603 510 402
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1332 2151
Size: 1332 819
BstYI r'GATC_y
Cuts at: 0 177 585 1314 1352 1653 2151
Size: 177 408 729 38 301 498
Fragments arranged by size:
729 498 408 301 177 38
Bsu36I CC'TnA_GG
Cuts at: 0 83 1885 2151
Size: 83 1802 266
Fragments arranged by size:
1802 266 83
BtgI C'CryG_G
Cuts at: 0 1282 1331 1488 1778 1832 2097 2151
Size: 1282 49 157 290 54 265 54
Fragments arranged by size:
1282 290 265 157 54 54 49
BtsI GCAGTG_nn'
Cuts at: 0 698 2151
Size: 698 1453
EaeI y'GGCC_r
Cuts at: 0 1017 1427 1849 2151
Size: 1017 410 422 302
Fragments arranged by size:
1017 422 410 302
EagI C'GGCC_G
Cuts at: 0 1427 2151
Size: 1427 724
EarI CTCTTCn'nnn_
Cuts at: 0 272 2151
Size: 272 1879
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1240 1740 2151
Size: 1240 500 411
Fragments arranged by size:
1240 500 411
EcoICRI GAG'CTC
Cuts at: 0 330 456 1368 2151
Size: 330 126 912 783
Fragments arranged by size:
912 783 330 126
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 145 220 235 328 610 695 741 754
Size: 145 75 15 93 282 85 46 13
Cuts at: 754 784 826 898 949 1021 1063 1105 1198
Size: 30 42 72 51 72 42 42 93
Cuts at: 1198 1375 1417 1486 1613 1642 1678 1714 1853
Size: 177 42 69 127 29 36 36 139
Cuts at: 1853 1948 2151
Size: 95 203
Fragments arranged by size:
282 203 177 145 139 127 95 93
93 85 75 72 72 69 51 46
42 42 42 42 36 36 30 29
15 13
EcoNI CCTnn'n_nnAGG
Cuts at: 0 541 613 859 2151
Size: 541 72 246 1292
Fragments arranged by size:
1292 541 246 72
EcoO109I rG'GnC_Cy
Cuts at: 0 87 184 873 1554 2151
Size: 87 97 689 681 597
Fragments arranged by size:
689 681 597 97 87
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 240 2151
Size: 240 1911
FseI GG_CCGG'CC
Cuts at: 0 672 2151
Size: 672 1479
HaeII r_GCGC'y
Cuts at: 0 579 789 847 957 1461 2044 2151
Size: 579 210 58 110 504 583 107
Fragments arranged by size:
583 579 504 210 110 107 58
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 469 501 502 534 1306 1338 2151
Size: 469 32 1 32 772 32 813
Fragments arranged by size:
813 772 469 32 32 32 1
HincII GTy'rAC
Cuts at: 0 1292 2151
Size: 1292 859
HindIII A'AGCT_T
Cuts at: 0 1639 2151
Size: 1639 512
KasI G'GCGC_C
Cuts at: 0 953 2040 2151
Size: 953 1087 111
Fragments arranged by size:
1087 953 111
KpnI G_GTAC'C
Cuts at: 0 689 2151
Size: 689 1462
MfeI C'AATT_G
Cuts at: 0 739 2151
Size: 739 1412
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1475 1944 2151
Size: 1475 469 207
Fragments arranged by size:
1475 469 207
MslI CAynn'nnrTG
Cuts at: 0 1330 1822 2151
Size: 1330 492 329
Fragments arranged by size:
1330 492 329
MspA1I CmG'CkG
Cuts at: 0 98 348 600 732 927 999 1083 1095
Size: 98 250 252 132 195 72 84 12
Cuts at: 1095 1125 1275 1284 1395 1848 1855 1959 2076
Size: 30 150 9 111 453 7 104 117
Cuts at: 2076 2151
Size: 75
Fragments arranged by size:
453 252 250 195 150 132 117 111
104 98 84 75 72 30 12 9
7
NaeI GCC'GGC
Cuts at: 0 670 952 1017 2151
Size: 670 282 65 1134
Fragments arranged by size:
1134 670 282 65
NarI GG'CG_CC
Cuts at: 0 954 2041 2151
Size: 954 1087 110
Fragments arranged by size:
1087 954 110
NcoI C'CATG_G
Cuts at: 0 1778 1832 2151
Size: 1778 54 319
Fragments arranged by size:
1778 319 54
NgoMIV G'CCGG_C
Cuts at: 0 668 950 1015 2151
Size: 668 282 65 1136
Fragments arranged by size:
1136 668 282 65
NspI r_CATG'y
Cuts at: 0 433 2151
Size: 433 1718
PflMI CCAn_nnn'nTGG
Cuts at: 0 1494 2151
Size: 1494 657
PpuMI rG'GwC_Cy
Cuts at: 0 87 184 1554 2151
Size: 87 97 1370 597
Fragments arranged by size:
1370 597 97 87
PspOMI G'GGCC_C
Cuts at: 0 1541 2151
Size: 1541 610
PstI C_TGCA'G
Cuts at: 0 830 1118 1280 1457 1562 1574 1742 2151
Size: 830 288 162 177 105 12 168 409
Fragments arranged by size:
830 409 288 177 168 162 105 12
PvuII CAG'CTG
Cuts at: 0 348 600 732 927 999 1083 1275 1395
Size: 348 252 132 195 72 84 192 120
Cuts at: 1395 1855 2151
Size: 460 296
Fragments arranged by size:
460 348 296 252 195 192 132 120
84 72
SacI G_AGCT'C
Cuts at: 0 332 458 1370 2151
Size: 332 126 912 781
Fragments arranged by size:
912 781 332 126
SacII CC_GC'GG
Cuts at: 0 1285 2151
Size: 1285 866
SapI GCTCTTCn'nnn_
Cuts at: 0 272 2151
Size: 272 1879
SbfI CC_TGCA'GG
Cuts at: 0 1574 2151
Size: 1574 577
ScaI AGT'ACT
Cuts at: 0 244 2151
Size: 244 1907
SexAI A'CCwGG_T
Cuts at: 0 40 2151
Size: 40 2111
SfcI C'TryA_G
Cuts at: 0 826 1114 1276 1453 1558 1570 1738 2151
Size: 826 288 162 177 105 12 168 413
Fragments arranged by size:
826 413 288 177 168 162 105 12
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1144 2151
Size: 1144 1007
SfoI GGC'GCC
Cuts at: 0 955 2042 2151
Size: 955 1087 109
Fragments arranged by size:
1087 955 109
StyI C'CwwG_G
Cuts at: 0 1778 1832 2105 2151
Size: 1778 54 273 46
Fragments arranged by size:
1778 273 54 46
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1716 2151
Size: 1716 435
TatI w'GTAC_w
Cuts at: 0 242 1679 2151
Size: 242 1437 472
Fragments arranged by size:
1437 472 242
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 188 2151
Size: 188 1963
XmnI GAAnn'nnTTC
Cuts at: 0 422 2151
Size: 422 1729
Enzymes that do cut:
AarI Acc65I AcuI AfeI AhdI AleI AlfI AloI
AlwNI ApaI BamHI BanI BanII BbeI BbsI BbvCI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI
BsrFI BssHII BstAPI BstXI BstYI Bsu36I BtgI BtsI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
FalI FseI HaeII Hin4I HincII HindIII KasI KpnI
MfeI MmeI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PflMI PpuMI PspOMI PstI PvuII SacI SacII
SapI SbfI ScaI SexAI SfcI SfiI SfoI StyI
TaqII TatI XcmI XmnI
Enzymes that do not cut:
AatII AccI AclI AflII AflIII AgeI ApaLI ApoI
AscI AseI AsiSI AvaI AvrII BaeI BcgI BciVI
BclI BfrBI BmgBI BmtI BpuEI BsaBI BsiWI BspEI
BsrDI BsrGI BssSI BstBI BstEII BstZ17I BtgZI ClaI
CspCI DraI DraIII DrdI EcoRI EcoRV FspI FspAI
HpaI MluI MscI NdeI NheI NotI NruI NsiI
PacI PasI PciI PfoI PmeI PmlI PpiI PshAI
PsiI PspXI PsrI PvuI RsrII SalI SanDI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
StuI SwaI TstI Tth111I XbaI XhoI XmaI ZraI