Restriction map for
pFN21AB8515
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# NO CUT ENZYMES ( 70 enzymes) #
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Acc65I AclI AgeI AhdI Alw44I
ApaLI AscI AseI AsnI Asp718I
AspEI AspI AvrII BciVI BclI
BglII BlnI BsiWI BsmI Bsp1407I
BspLU11I BsrGI BssHII BssSI Bst1107I
BstBI BstZ17I DraI Eam1105I EcoRV
EcoT22I FbaI HindIII HpaI KpnI
MfeI MluI MunI NcoI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmeI
PshAI PshBI Psp1406I PvuI SalI
SbfI SexAI SfiI SfuI SgrAI
SnaBI SpeI Sse8387I SspBI SspI
StuI SwaI Tth111I XbaI XhoI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AccI 2227 GTCTAC GT^[AC][GT]AC
AccIII 1574 TCCGGA T^CCGGA
AflII 1201 CTTAAG C^TTAAG
AocI 2368 CCTGAGG CC^T[ACGT]AGG
Aor51HI 736 AGCGCT AGC^GCT
AviII 656 TGCGCA TGC^GCA
BalI 422 TGGCCA TGG^CCA
BbeI 2672 GGCGCC GGCGC^C
BbsI 2485 GTCTTC GAAGAC(2/6)
BbvCI 1432 GCTGAGG CCTCAGC(-5/-2)
BcgI 2601 CGAGAGAGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 1201 CTTAAG C^TTAAG
BlpI 732 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 732 GCTCAGC GC^T[ACGT]AGC
BpuAI 2485 GTCTTC GAAGAC(2/6)
BseAI 1574 TCCGGA T^CCGGA
BsmBI 1979 GAGACG CGTCTC(1/5)
BspDI 1546 ATCGAT AT^CGAT
BspEI 1574 TCCGGA T^CCGGA
BspHI 2552 TCATGA T^CATGA
BsrDI 476 GCAATG GCAATG(2/0)
BstEII 1437 GGTCACC G^GT[ACGT]ACC
BstPI 1437 GGTCACC G^GT[ACGT]ACC
Bsu36I 2368 CCTGAGG CC^T[ACGT]AGG
CelII 732 GCTCAGC GC^T[ACGT]AGC
ClaI 1546 ATCGAT AT^CGAT
CpoI 752 CGGACCG CG^G[AT]CCG
Eco47III 736 AGCGCT AGC^GCT
Eco81I 2368 CCTGAGG CC^T[ACGT]AGG
EcoO65I 1437 GGTCACC G^GT[ACGT]ACC
EcoRI 1915 GAATTC G^AATTC
FseI 585 GGCCGGCC GGCCGG^CC
FspI 656 TGCGCA TGC^GCA
HgaI 2776 GACGC GACGC(5/10)
HincII 2500 GTCAAC GT[CT]^[GA]AC
HindII 2500 GTCAAC GT[CT]^[GA]AC
KasI 2672 GGCGCC G^GCGCC
KspI 2795 CCGCGG CCGC^GG
MluNI 422 TGGCCA TGG^CCA
MroI 1574 TCCGGA T^CCGGA
MscI 422 TGGCCA TGG^CCA
MseI 1202 TTAA T^TAA
NaeI 586 GCCGGC GCC^GGC
NarI 2672 GGCGCC GG^CGCC
NgoMIV 586 GCCGGC G^CCGGC
NspI 560 GCATGC [GA]CATG^[CT]
RcaI 2552 TCATGA T^CATGA
RsrII 752 CGGACCG CG^G[AT]CCG
SacII 2795 CCGCGG CCGC^GG
ScaI 2105 AGTACT AGT^ACT
SfoI 2672 GGCGCC GGC^GCC
SmlI 1201 CTTAAG C^T[CT][GA]AG
SphI 560 GCATGC GCATG^C
Tru9I 1202 TTAA T^TAA
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# TWO CUT ENZYMES ( 14 enzymes) #
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AatII 1230 GACGTC GACGT^C
2716 GACGTC
ApaI 228 GGGCCC GGGCC^C
934 GGGCCC
BamHI 1633 GGATCC G^GATCC
1722 GGATCC
BsaBI 546 GATTGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1539 GATGAACATC
BsrFI 586 GCCGGC [GA]^CCGG[CT]
2113 GCCGGT
BstXI 206 CCACTGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
348 CCAGTCCCCTGG
Cfr10I 586 GCCGGC [GA]^CCGG[CT]
2113 GCCGGT
DrdI 2379 GACGGACACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2491 GACAGCATCGTC
EcoNI 2298 CCTCTGCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2633 CCTTCTTCAGG
MamI 546 GATTGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1539 GATGAACATC
SmaI 103 CCCGGG CCC^GGG
1112 CCCGGG
XcmI 203 CCACCACTGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
797 CCAACCAGAAGCTGG
XmaCI 103 CCCGGG C^CCGGG
1112 CCCGGG
XmaI 103 CCCGGG C^CCGGG
1112 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 120 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI ApoI
Asp700I AspHI AvaI AvaII BanI
BanII BbrPI BbvI BcnI BfaI
BglI BmyI BpmI BsaAI BsaHI
BsaI BsaJI BsaWI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrI BssKI BstAPI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII DraIII DsaI
EaeI EagI EarI EclXI Eco52I
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PflMI
PleI PmaCI PmlI PpuMI PstI
PvuII RsaI SacI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TaqI TfiI TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XhoII XmnI