(Circular) (Six-base) MAPSORT of: pFN21AB9993.seq Check: 9625 from: 1 to: 6457 pFN21AB9993 6457 bp With 182 enzymes: SgfI * August 27, 2010 15:51 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4267 278 Size: 53 83 186 936 2731 2468 Fragments arranged by size: 2731 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3096 3730 3800 1937 Size: 1159 634 70 4594 Fragments arranged by size: 4594 1159 634 70 Acc65I G'GTAC_C Cuts at: 3778 3778 Size: 6457 AclI AA'CG_TT Cuts at: 1563 4584 4957 1563 Size: 3021 373 3063 Fragments arranged by size: 3063 3021 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4520 5904 6172 1632 Size: 2888 1384 268 1917 Fragments arranged by size: 2888 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6260 AflIII A'CryG_T Cuts at: 1203 5356 6333 1203 Size: 4153 977 1327 Fragments arranged by size: 4153 1327 977 AgeI A'CCGG_T Cuts at: 5288 5288 Size: 6457 AhdI GACnn_n'nnGTC Cuts at: 5186 5186 Size: 6457 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6457 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3248 1184 Size: 2064 4393 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3455 3487 3455 Size: 32 6425 AlwNI CAG_nnn'CTG Cuts at: 3131 4200 5772 3131 Size: 1069 1572 3816 Fragments arranged by size: 3816 1572 1069 ApaI G_GGCC'C Cuts at: 2136 3342 2136 Size: 1206 5251 ApaLI G'TGCA_C Cuts at: 3366 4514 5670 3366 Size: 1148 1156 4153 Fragments arranged by size: 4153 1156 1148 ApoI r'AATT_y Cuts at: 1493 2681 3766 3992 4144 6253 1493 Size: 1188 1085 226 152 2109 1697 Fragments arranged by size: 2109 1697 1188 1085 226 152 AseI AT'TA_AT Cuts at: 160 1032 5011 160 Size: 872 3979 1606 Fragments arranged by size: 3979 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6457 AvaI C'yCGr_G Cuts at: 1870 1943 2370 2477 3064 3372 3782 1870 Size: 73 427 107 587 308 410 4545 Fragments arranged by size: 4545 587 427 410 308 107 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2491 2524 2491 Size: 33 6424 BamHI G'GATC_C Cuts at: 2803 3787 4154 2803 Size: 984 367 5106 Fragments arranged by size: 5106 984 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2232 3778 5233 618 Size: 325 217 627 445 1546 1455 1842 Fragments arranged by size: 1842 1546 1455 627 445 325 217 BanII G_rGCy'C Cuts at: 1391 2136 3342 3468 3776 1391 Size: 745 1206 126 308 4072 Fragments arranged by size: 4072 1206 745 308 126 BbeI G_GCGC'C Cuts at: 2236 2236 Size: 6457 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5486 BbvCI CC'TCA_GC Cuts at: 3501 3501 Size: 6457 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2873 2907 4648 4682 1903 Size: 34 936 34 1741 34 3678 Fragments arranged by size: 3678 1741 936 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4349 5559 4349 Size: 1210 5247 BclI T'GATC_A Cuts at: 1548 1548 Size: 6457 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2222 5068 136 Size: 107 122 71 631 605 550 2846 1525 Fragments arranged by size: 2846 1525 631 605 550 122 107 71 BglII A'GATC_T Cuts at: 6452 6452 Size: 6457 BlpI GC'TnA_GC Cuts at: 2651 3007 3447 3485 3874 2651 Size: 356 440 38 389 5234 Fragments arranged by size: 5234 440 389 356 38 Bme1580I G_kGCm'C Cuts at: 1790 2136 2539 3342 3370 4518 5674 1790 Size: 346 403 803 28 1148 1156 2573 Fragments arranged by size: 2573 1156 1148 803 403 346 28 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6457 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3424 4188 5146 Size: 416 53 83 399 24 2007 764 958 Cuts at: 5146 442 Size: 1753 Fragments arranged by size: 2007 1753 958 764 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6457 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2087 2312 2507 2894 3108 5117 1239 Size: 848 225 195 387 214 2009 2579 Fragments arranged by size: 2579 2009 848 387 225 214 195 Bpu10I CC'TnA_GC Cuts at: 2610 3501 3597 2610 Size: 891 96 5470 Fragments arranged by size: 5470 891 96 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4582 5447 5745 4582 Size: 865 298 5294 Fragments arranged by size: 5294 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5120 882 Size: 617 3621 2219 Fragments arranged by size: 3621 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4248 493 Size: 711 3044 2702 Fragments arranged by size: 3044 2702 711 BsaBI GATnn'nnATC Cuts at: 6451 6451 Size: 6457 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2233 4264 4646 5281 Size: 53 83 186 936 700 2031 382 635 Cuts at: 5281 275 Size: 1451 Fragments arranged by size: 2031 1451 936 700 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2043 2806 4890 5288 5562 5709 6120 2043 Size: 763 2084 398 274 147 411 2380 Fragments arranged by size: 2380 2084 763 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2310 2340 3455 3485 1178 Size: 30 165 30 907 30 1115 30 4150 Fragments arranged by size: 4150 1115 907 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2114 2136 2315 2414 2641 2683 3035 3038 Size: 928 22 179 99 227 42 352 3 Cuts at: 3038 1186 Size: 4605 Fragments arranged by size: 4605 928 352 227 179 99 42 22 3 BseYI C'CCAG_C Cuts at: 2225 3164 3335 5660 6156 2225 Size: 939 171 2325 496 2526 Fragments arranged by size: 2526 2325 939 496 171 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3129 1163 Size: 573 1393 4491 Fragments arranged by size: 4491 1393 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3907 4668 5696 1540 Size: 275 186 1906 761 1028 2301 Fragments arranged by size: 2301 1906 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2183 3370 3548 3573 4518 4603 5674 2183 Size: 1187 178 25 945 85 1071 2966 Fragments arranged by size: 2966 1187 1071 945 178 85 25 BsmI GAATG_Cn' Cuts at: 2697 3968 4061 2697 Size: 1271 93 5093 Fragments arranged by size: 5093 1271 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2716 2716 Size: 6457 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2136 2183 2539 3342 3370 3468 3548 Size: 399 346 47 356 803 28 98 80 Cuts at: 3548 3573 3776 4518 4603 5674 1391 Size: 25 203 742 85 1071 2174 Fragments arranged by size: 2174 1071 803 742 399 356 346 203 98 85 80 47 28 25 BspEI T'CCGG_A Cuts at: 2043 2806 6120 2043 Size: 763 3314 2380 Fragments arranged by size: 3314 2380 763 BspHI T'CATG_A Cuts at: 2980 4346 6076 2980 Size: 1366 1730 3361 Fragments arranged by size: 3361 1730 1366 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3812 844 Size: 2968 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2650 3122 3748 4344 1394 Size: 429 827 472 626 596 3507 Fragments arranged by size: 3507 827 626 596 472 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3182 4952 5126 65 Size: 1183 1934 1770 174 1396 Fragments arranged by size: 1934 1770 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3489 5101 5288 1700 Size: 110 1679 1612 187 2869 Fragments arranged by size: 2869 1679 1612 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6457 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6457 BssSI C'ACGA_G Cuts at: 4517 5529 4517 Size: 1012 5445 BstAPI GCAn_nnn'nTGC Cuts at: 794 3152 794 Size: 2358 4099 BstBI TT'CG_AA Cuts at: 3399 5261 3399 Size: 1862 4595 BstEII G'GTnAC_C Cuts at: 1184 2563 2971 1184 Size: 1379 408 4670 Fragments arranged by size: 4670 1379 408 BstXI CCAn_nnnn'nTGG Cuts at: 2584 3172 2584 Size: 588 5869 BstYI r'GATC_y Cuts at: 1972 2008 2803 3211 3787 4154 4539 4556 6008 Size: 36 795 408 576 367 385 17 1452 Cuts at: 6008 6094 6106 6452 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 795 576 408 385 367 346 86 36 17 12 Bsu36I CC'TnA_GG Cuts at: 2207 2951 3621 2207 Size: 744 670 5043 Fragments arranged by size: 5043 744 670 BtgI C'CryG_G Cuts at: 513 1065 2004 2413 3441 3475 513 Size: 552 939 409 1028 34 3495 Fragments arranged by size: 3495 1028 939 552 409 34 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2046 2743 487 Size: 683 56 820 697 4201 Fragments arranged by size: 4201 820 697 683 56 BtsI GCAGTG_nn' Cuts at: 3141 3330 3370 3506 3973 4767 4787 3141 Size: 189 40 136 467 794 20 4811 Fragments arranged by size: 4811 794 467 189 136 40 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6457 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2329 2364 529 Size: 35 1765 35 4622 Fragments arranged by size: 4622 1765 35 35 DraI TTT'AAA Cuts at: 3761 3761 Size: 6457 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5464 809 Size: 507 4148 1802 Fragments arranged by size: 4148 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2214 2736 2917 Size: 54 1425 50 275 18 384 522 181 Cuts at: 2917 3559 3904 4793 6422 8 Size: 642 345 889 1629 43 Fragments arranged by size: 1629 1425 889 642 522 384 345 275 181 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3904 1537 Size: 275 2092 4090 Fragments arranged by size: 4090 2092 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3024 3414 4387 1353 Size: 1671 390 973 3423 Fragments arranged by size: 3423 1671 973 390 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5044 5418 5564 387 Size: 1064 138 3455 374 146 1280 Fragments arranged by size: 3455 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2087 2312 2507 2894 3108 4520 5117 Size: 393 455 225 195 387 214 1412 597 Cuts at: 5117 5904 6172 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1412 787 597 455 393 387 268 225 214 195 EcoNI CCTnn'n_nnAGG Cuts at: 2327 3308 2327 Size: 981 5476 EcoO109I rG'GnC_Cy Cuts at: 2132 2312 2474 2518 3339 3376 2132 Size: 180 162 44 821 37 5213 Fragments arranged by size: 5213 821 180 162 44 37 EcoRI G'AATT_C Cuts at: 3766 4144 3766 Size: 378 6079 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3425 3425 Size: 6457 FspI TGC'GCA Cuts at: 4963 4963 Size: 6457 HaeII r_GCGC'y Cuts at: 1678 2236 5604 1678 Size: 558 3368 2531 Fragments arranged by size: 3368 2531 558 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2042 2074 3223 3255 3266 Size: 32 135 32 470 32 1149 32 11 Cuts at: 3266 3298 3628 3660 5098 5130 5172 5204 1373 Size: 32 330 32 1438 32 42 32 2626 Fragments arranged by size: 2626 1438 1149 470 330 135 42 32 32 32 32 32 32 32 32 11 HincII GTy'rAC Cuts at: 669 1657 1938 3292 3801 4047 669 Size: 988 281 1354 509 246 3079 Fragments arranged by size: 3079 1354 988 509 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6457 HpaI GTT'AAC Cuts at: 4047 4047 Size: 6457 KasI G'GCGC_C Cuts at: 2232 2232 Size: 6457 KpnI G_GTAC'C Cuts at: 3782 3782 Size: 6457 MfeI C'AATT_G Cuts at: 4056 4056 Size: 6457 MluI A'CGCG_T Cuts at: 6333 6333 Size: 6457 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5571 5755 1305 Size: 4266 184 2007 Fragments arranged by size: 4266 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2216 2919 3561 10 Size: 54 1425 343 384 703 642 2906 Fragments arranged by size: 2906 1425 703 642 384 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4415 4774 4933 518 Size: 648 3249 359 159 2042 Fragments arranged by size: 3249 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2267 3873 4550 5698 5943 1606 Size: 661 1606 677 1148 245 2120 Fragments arranged by size: 2120 1606 1148 677 661 245 NaeI GCC'GGC Cuts at: 3491 3491 Size: 6457 NarI GG'CG_CC Cuts at: 2233 2233 Size: 6457 NcoI C'CATG_G Cuts at: 513 1065 2004 3441 3475 513 Size: 552 939 1437 34 3495 Fragments arranged by size: 3495 1437 939 552 34 NdeI CA'TA_TG Cuts at: 387 387 Size: 6457 NgoMIV G'CCGG_C Cuts at: 3489 3489 Size: 6457 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6457 NotI GC'GGCC_GC Cuts at: 3904 3904 Size: 6457 NspI r_CATG'y Cuts at: 1132 1229 2776 3815 5360 1132 Size: 97 1547 1039 1545 2229 Fragments arranged by size: 2229 1547 1545 1039 97 PasI CC'CwG_GG Cuts at: 1348 2128 2237 1348 Size: 780 109 5568 Fragments arranged by size: 5568 780 109 PciI A'CATG_T Cuts at: 5356 5356 Size: 6457 PflMI CCAn_nnn'nTGG Cuts at: 1108 2872 1108 Size: 1764 4693 PfoI T'CCnGG_A Cuts at: 2328 2328 Size: 6457 PmeI GTTT'AAAC Cuts at: 3761 3761 Size: 6457 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3455 3487 4526 4558 6039 6071 3455 Size: 32 1039 32 1481 32 3841 Fragments arranged by size: 3841 1481 1039 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2474 2518 3376 2474 Size: 44 858 5555 Fragments arranged by size: 5555 858 44 PshAI GACnn'nnGTC Cuts at: 1613 2163 2561 1613 Size: 550 398 5509 Fragments arranged by size: 5509 550 398 PsiI TTA'TAA Cuts at: 4027 4027 Size: 6457 PspOMI G'GGCC_C Cuts at: 2132 3338 2132 Size: 1206 5251 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3714 3746 3714 Size: 32 6425 PstI C_TGCA'G Cuts at: 830 3516 3809 830 Size: 2686 293 3478 Fragments arranged by size: 3478 2686 293 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6457 PvuII CAG'CTG Cuts at: 2267 2267 Size: 6457 SalI G'TCGA_C Cuts at: 1936 3799 1936 Size: 1863 4594 SanDI GG'GwC_CC Cuts at: 2474 2474 Size: 6457 SapI GCTCTTCn'nnn_ Cuts at: 3024 3414 3024 Size: 390 6067 SbfI CC_TGCA'GG Cuts at: 3809 3809 Size: 6457 ScaI AGT'ACT Cuts at: 3050 4705 6417 3050 Size: 1655 1712 3090 Fragments arranged by size: 3090 1712 1655 SfcI C'TryA_G Cuts at: 826 1045 2638 3189 3512 3731 3805 4940 5621 Size: 219 1593 551 323 219 74 1135 681 Cuts at: 5621 5812 826 Size: 191 1471 Fragments arranged by size: 1593 1471 1135 681 551 323 219 219 191 74 SfoI GGC'GCC Cuts at: 2234 2234 Size: 6457 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6457 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6457 SmaI CCC'GGG Cuts at: 1872 2479 3066 3374 3784 1872 Size: 607 587 308 410 4545 Fragments arranged by size: 4545 607 587 410 308 SmlI C'TyrA_G Cuts at: 820 1017 1943 4561 5462 5724 820 Size: 197 926 2618 901 262 1553 Fragments arranged by size: 2618 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4248 493 Size: 3755 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6457 SphI G_CATG'C Cuts at: 1132 3815 1132 Size: 2683 3774 SspI AAT'ATT Cuts at: 5 52 4381 5 Size: 47 4329 2081 Fragments arranged by size: 4329 2081 47 StuI AGG'CCT Cuts at: 1517 2924 1517 Size: 1407 5050 StyI C'CwwG_G Cuts at: 513 1065 2004 3081 3441 3475 3896 513 Size: 552 939 1077 360 34 421 3074 Fragments arranged by size: 3074 1077 939 552 421 360 34 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2977 3163 3317 4484 4501 4654 4839 Size: 1255 1397 186 154 1167 17 153 185 Cuts at: 4839 5284 325 Size: 445 1498 Fragments arranged by size: 1498 1397 1255 1167 445 186 185 154 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2031 3048 4703 Size: 274 80 33 51 1443 54 1017 1655 Cuts at: 4703 6415 96 Size: 1712 138 Fragments arranged by size: 1712 1655 1443 1017 274 138 80 54 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6417 6449 6417 Size: 32 6425 Tth111I GACn'n_nGTC Cuts at: 1316 2465 1316 Size: 1149 5308 XbaI T'CTAG_A Cuts at: 3793 3793 Size: 6457 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6457 XmaI C'CCGG_G Cuts at: 1870 2477 3064 3372 3782 1870 Size: 607 587 308 410 4545 Fragments arranged by size: 4545 607 587 410 308 XmnI GAAnn'nnTTC Cuts at: 3395 4586 3395 Size: 1191 5266 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4265 276 Size: 53 83 186 936 2731 2468 Fragments arranged by size: 2731 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BfrBI BplI BsiWI BstZ17I DraIII EcoICRI EcoRV FseI FspAI NruI NsiI PacI PmlI PspXI RsrII SacI SacII SexAI SfiI SrfI SwaI XcmI