Restriction map for
pFN21AB8491
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# NO CUT ENZYMES ( 105 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AfaI AflIII AgeI AhdI Alw44I
Aor51HI ApaLI AscI AseI AsnI
Asp718I AspEI AspI AviII AvrII
BbeI BbsI BcgI BclI BlnI
BpuAI BsaBI BsaHI BsaWI BseAI
BsiWI Bsp1407I BspDI BspEI BspLU11I
BsrDI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstZ17I ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoO65I
EcoRI EcoRV FbaI FseI FspI
HgaI Hin1I HpaI KasI KpnI
KspI MamI MfeI MluI MroI
MunI NarI NdeI NotI NruI
NspI NspV PacI PaeR7I PflFI
PinAI PmeI PshAI PshBI Psp1406I
PvuI RsaI RsrII SacII ScaI
SexAI SfoI SfuI SgrAI SnaBI
SpeI SphI SspBI SspI StuI
SwaI Tth111I XbaI XcmI XhoI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AccI 2185 GTCGAC GT^[AC][GT]AC
AccII 1589 CGCG CG^CG
AcsI 2130 GAATTT [GA]^AATT[CT]
AflII 1105 CTTAAG C^TTAAG
ApoI 2130 GAATTT [GA]^AATT[CT]
Asp700I 298 GAAAGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 1487 TGGCCA TGG^CCA
BanI 2663 GGCACC G^G[CT][GA]CC
BbrPI 338 CACGTG CAC^GTG
BciVI 1257 GGATAC GTATCC(6/5)
BfrI 1105 CTTAAG C^TTAAG
BglI 862 GCCATGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1905 AGATCT A^GATCT
BsaI 1198 GAGACC GGTCTC(1/5)
BsiEI 672 CGACCG CG[GA][CT]^CG
BsmBI 387 GAGACG CGTCTC(1/5)
BsmI 1944 GCATTC GAATGC(1/-1)
BspHI 192 TCATGA T^CATGA
BspMI 1545 GCAGGT ACCTGC(4/8)
BstUI 1589 CGCG CG^CG
BstXI 1811 CCATGGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
EcoT22I 563 ATGCAT ATGCA^T
HincII 2185 GTCGAC GT[CT]^[GA]AC
HindII 2185 GTCGAC GT[CT]^[GA]AC
HindIII 231 AAGCTT A^AGCTT
MluNI 1487 TGGCCA TGG^CCA
MscI 1487 TGGCCA TGG^CCA
MseI 1106 TTAA T^TAA
MvnI 1589 CGCG CG^CG
NaeI 134 GCCGGC GCC^GGC
NgoMIV 134 GCCGGC G^CCGGC
NheI 1497 GCTAGC G^CTAGC
NsiI 563 ATGCAT ATGCA^T
PmaCI 338 CACGTG CAC^GTG
PmlI 338 CACGTG CAC^GTG
RcaI 192 TCATGA T^CATGA
SalI 2185 GTCGAC G^TCGAC
SbfI 987 CCTGCAGG CCTGCA^GG
SfiI 861 GGCCATGGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
Sse8387I 987 CCTGCAGG CCTGCA^GG
Tru9I 1106 TTAA T^TAA
XmnI 298 GAAAGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 14 enzymes) #
###################################
ApaI 1085 GGGCCC GGGCC^C
1923 GGGCCC
BamHI 357 GGATCC G^GATCC
1331 GGATCC
BsaAI 338 CACGTG [CT]AC^GT[GA]
2252 CACGTA
BsrFI 134 GCCGGC [GA]^CCGG[CT]
740 ACCGGC
Cfr10I 134 GCCGGC [GA]^CCGG[CT]
740 ACCGGC
MaeII 339 ACGT A^CGT
2253 ACGT
MslI 1678 CACTGGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1812 CATGGCCGTG
PpuMI 1457 AGGACCT [GA]G^G[AT]CC[CT]
2315 GGGTCCC
SacI 802 GAGCTC GAGCT^C
1564 GAGCTC
SapI 994 GAAGAGC GCTCTTC(1/4)
1167 GAAGAGC
SmaI 1577 CCCGGG CCC^GGG
1759 CCCGGG
Tsp45I 100 GTGAC ^GT[GC]AC
1375 GTGAC
XmaCI 1577 CCCGGG C^CCGGG
1759 CCCGGG
XmaI 1577 CCCGGG C^CCGGG
1759 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 97 enzymes) #
#########################################
AciI AluI AlwI AlwNI AocI
AspHI AvaI AvaII BanII BbvCI
BbvI BcnI BfaI BlpI BmyI
BpmI Bpu1102I BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrBI BsrI BssKI
BstAPI BstF5I BstNI BstYI Bsu36I
Cac8I CelII CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MflI MnlI MspA1I
MspI MvaI MwoI NciI NcoI
NdeII NlaIII NlaIV PflMI PleI
PstI PvuII Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI TseI Tsp509I TspRI TthHB8I
Van91I XhoII