Restriction map for
pFN21AB8491
(Linear) (Six-base) MAPSORT of: KIBB8491.seq Check: 6937 from: 1 to: 2709
KIBB8491.orf
With 182 enzymes: SgfI *
October 7, 2009 14:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1536 2709
Size: 1536 1173
AccI GT'mk_AC
Cuts at: 0 2186 2709
Size: 2186 523
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 116 1846 2512 2709
Size: 116 1730 666 197
Fragments arranged by size:
1730 666 197 116
AflII C'TTAA_G
Cuts at: 0 1105 2709
Size: 1105 1604
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 908 2709
Size: 908 1801
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2539 2571 2709
Size: 2539 32 138
Fragments arranged by size:
2539 138 32
AlwNI CAG_nnn'CTG
Cuts at: 0 555 618 1125 1461 1485 1623 1959 2148
Size: 555 63 507 336 24 138 336 189
Cuts at: 2148 2709
Size: 561
Fragments arranged by size:
561 555 507 336 336 189 138 63
24
ApaI G_GGCC'C
Cuts at: 0 1089 1927 2709
Size: 1089 838 782
Fragments arranged by size:
1089 838 782
ApoI r'AATT_y
Cuts at: 0 2130 2709
Size: 2130 579
AvaI C'yCGr_G
Cuts at: 0 34 155 626 1010 1577 1759 2447 2673
Size: 34 121 471 384 567 182 688 226
Cuts at: 2673 2709
Size: 36
Fragments arranged by size:
688 567 471 384 226 182 121 36
34
BamHI G'GATC_C
Cuts at: 0 357 1331 2709
Size: 357 974 1378
Fragments arranged by size:
1378 974 357
BanI G'GyrC_C
Cuts at: 0 2663 2709
Size: 2663 46
BanII G_rGCy'C
Cuts at: 0 80 162 806 1089 1568 1927 1967 2463
Size: 80 82 644 283 479 359 40 496
Cuts at: 2463 2709
Size: 246
Fragments arranged by size:
644 496 479 359 283 246 82 80
40
BbvCI CC'TCA_GC
Cuts at: 0 130 1553 2471 2709
Size: 130 1423 918 238
Fragments arranged by size:
1423 918 238 130
BciVI GTATCCnnnnn_n'
Cuts at: 0 1251 2709
Size: 1251 1458
BfrBI ATG'CAT
Cuts at: 0 565 2709
Size: 565 2144
BglI GCCn_nnn'nGGC
Cuts at: 0 868 2709
Size: 868 1841
BglII A'GATC_T
Cuts at: 0 1905 2709
Size: 1905 804
BlpI GC'TnA_GC
Cuts at: 0 471 752 1702 2111 2709
Size: 471 281 950 409 598
Fragments arranged by size:
950 598 471 409 281
Bme1580I G_kGCm'C
Cuts at: 0 1089 1927 2162 2666 2709
Size: 1089 838 235 504 43
Fragments arranged by size:
1089 838 504 235 43
BmrI ACTGGGnnnn_n'
Cuts at: 0 17 1689 2709
Size: 17 1672 1020
Fragments arranged by size:
1672 1020 17
BmtI G_CTAG'C
Cuts at: 0 1501 2709
Size: 1501 1208
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1160 2346 2607 2709
Size: 1160 1186 261 102
Fragments arranged by size:
1186 1160 261 102
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 301 505 946 1360 1384 1645 1737 1892
Size: 301 204 441 414 24 261 92 155
Cuts at: 1892 1981 2137 2170 2709
Size: 89 156 33 539
Fragments arranged by size:
539 441 414 301 261 204 156 155
92 89 33 24
Bpu10I CC'TnA_GC
Cuts at: 0 130 1397 1553 2289 2321 2471 2709
Size: 130 1267 156 736 32 150 238
Fragments arranged by size:
1267 736 238 156 150 130 32
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1715 2110 2228 2709
Size: 1715 395 118 481
Fragments arranged by size:
1715 481 395 118
BsaI GGTCTCn'nnnn_
Cuts at: 0 1192 2709
Size: 1192 1517
BsaAI yAC'GTr
Cuts at: 0 340 2254 2709
Size: 340 1914 455
Fragments arranged by size:
1914 455 340
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 478 508 1791 1821 2539 2569 2709
Size: 478 30 1283 30 718 30 140
Fragments arranged by size:
1283 718 478 140 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 502 943 1147 1642 1727 1798 1803 1890
Size: 502 441 204 495 85 71 5 87
Cuts at: 1890 2322 2700 2709
Size: 432 378 9
Fragments arranged by size:
502 495 441 432 378 204 87 85
71 9 5
BseYI C'CCAG_C
Cuts at: 0 107 161 1015 1088 1202 1993 2356 2453
Size: 107 54 854 73 114 791 363 97
Cuts at: 2453 2709
Size: 256
Fragments arranged by size:
854 791 363 256 114 107 97 73
54
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1243 1327 1378 1991 2709
Size: 1243 84 51 613 718
Fragments arranged by size:
1243 718 613 84 51
BsiEI CG_ry'CG
Cuts at: 0 675 2709
Size: 675 2034
BsiHKAI G_wGCw'C
Cuts at: 0 806 1100 1568 2709
Size: 806 294 468 1141
Fragments arranged by size:
1141 806 468 294
BsmI GAATG_Cn'
Cuts at: 0 1944 2709
Size: 1944 765
BsmBI CGTCTCn'nnnn_
Cuts at: 0 381 2709
Size: 381 2328
Bsp1286I G_dGCh'C
Cuts at: 0 80 162 806 1089 1100 1568 1927 1967
Size: 80 82 644 283 11 468 359 40
Cuts at: 1967 2162 2463 2666 2709
Size: 195 301 203 43
Fragments arranged by size:
644 468 359 301 283 203 195 82
80 43 40 11
BspHI T'CATG_A
Cuts at: 0 192 2709
Size: 192 2517
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1536 2709
Size: 1536 1173
BsrBI CCG'CTC
Cuts at: 0 756 999 1383 2709
Size: 756 243 384 1326
Fragments arranged by size:
1326 756 384 243
BsrFI r'CCGG_y
Cuts at: 0 134 740 2709
Size: 134 606 1969
Fragments arranged by size:
1969 606 134
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1665 2100 2145 2709
Size: 1665 435 45 564
Fragments arranged by size:
1665 564 435 45
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1818 2709
Size: 1818 891
BstYI r'GATC_y
Cuts at: 0 45 357 1331 1905 2061 2475 2570 2709
Size: 45 312 974 574 156 414 95 139
Fragments arranged by size:
974 574 414 312 156 139 95 45
Bsu36I CC'TnA_GG
Cuts at: 0 1429 1766 2054 2709
Size: 1429 337 288 655
Fragments arranged by size:
1429 655 337 288
BtgI C'CryG_G
Cuts at: 0 149 656 863 1811 1817 2294 2709
Size: 149 507 207 948 6 477 415
Fragments arranged by size:
948 507 477 415 207 149 6
BtsI GCAGTG_nn'
Cuts at: 0 2355 2367 2709
Size: 2355 12 342
Fragments arranged by size:
2355 342 12
EaeI y'GGCC_r
Cuts at: 0 517 1487 1814 2520 2709
Size: 517 970 327 706 189
Fragments arranged by size:
970 706 517 327 189
EarI CTCTTCn'nnn_
Cuts at: 0 389 989 1055 1162 2488 2709
Size: 389 600 66 107 1326 221
Fragments arranged by size:
1326 600 389 221 107 66
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 55 157 2709
Size: 55 102 2552
Fragments arranged by size:
2552 102 55
EcoICRI GAG'CTC
Cuts at: 0 804 1566 2709
Size: 804 762 1143
Fragments arranged by size:
1143 804 762
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 116 301 505 946 1360 1384 1645 1737
Size: 116 185 204 441 414 24 261 92
Cuts at: 1737 1846 1892 1981 2137 2170 2512 2709
Size: 109 46 89 156 33 342 197
Fragments arranged by size:
441 414 342 261 204 197 185 156
116 109 92 89 46 33 24
EcoNI CCTnn'n_nnAGG
Cuts at: 0 194 856 1428 2709
Size: 194 662 572 1281
Fragments arranged by size:
1281 662 572 194
EcoO109I rG'GnC_Cy
Cuts at: 0 523 870 1458 1924 1997 2015 2082 2121
Size: 523 347 588 466 73 18 67 39
Cuts at: 2121 2316 2709
Size: 195 393
Fragments arranged by size:
588 523 466 393 347 195 73 67
39 18
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 303 2709
Size: 303 2406
HaeII r_GCGC'y
Cuts at: 0 377 606 1126 1879 2709
Size: 377 229 520 753 830
Fragments arranged by size:
830 753 520 377 229
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1017 1049 1791 1823 2709
Size: 1017 32 742 32 886
Fragments arranged by size:
1017 886 742 32 32
HincII GTy'rAC
Cuts at: 0 2187 2709
Size: 2187 522
HindIII A'AGCT_T
Cuts at: 0 231 2709
Size: 231 2478
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 222 938 1022 1130 1325 2709
Size: 222 716 84 108 195 1384
Fragments arranged by size:
1384 716 222 195 108 84
MscI TGG'CCA
Cuts at: 0 1489 2709
Size: 1489 1220
MslI CAynn'nnrTG
Cuts at: 0 1682 1816 2709
Size: 1682 134 893
Fragments arranged by size:
1682 893 134
MspA1I CmG'CkG
Cuts at: 0 13 165 975 1290 1329 1338 1362 1371
Size: 13 152 810 315 39 9 24 9
Cuts at: 1371 1452 1623 1635 1698 1993 2115 2409 2510
Size: 81 171 12 63 295 122 294 101
Cuts at: 2510 2709
Size: 199
Fragments arranged by size:
810 315 295 294 199 171 152 122
101 81 63 39 24 13 12 9
9
NaeI GCC'GGC
Cuts at: 0 136 2709
Size: 136 2573
NcoI C'CATG_G
Cuts at: 0 863 1811 2294 2709
Size: 863 948 483 415
Fragments arranged by size:
948 863 483 415
NgoMIV G'CCGG_C
Cuts at: 0 134 2709
Size: 134 2575
NheI G'CTAG_C
Cuts at: 0 1497 2709
Size: 1497 1212
NsiI A_TGCA'T
Cuts at: 0 567 2709
Size: 567 2142
PasI CC'CwG_GG
Cuts at: 0 73 2709
Size: 73 2636
PflMI CCAn_nnn'nTGG
Cuts at: 0 483 1623 1959 2148 2709
Size: 483 1140 336 189 561
Fragments arranged by size:
1140 561 483 336 189
PfoI T'CCnGG_A
Cuts at: 0 2064 2709
Size: 2064 645
PmlI CAC'GTG
Cuts at: 0 340 2709
Size: 340 2369
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2539 2571 2709
Size: 2539 32 138
Fragments arranged by size:
2539 138 32
PpuMI rG'GwC_Cy
Cuts at: 0 1458 2316 2709
Size: 1458 858 393
Fragments arranged by size:
1458 858 393
PspOMI G'GGCC_C
Cuts at: 0 1085 1923 2709
Size: 1085 838 786
Fragments arranged by size:
1085 838 786
PstI C_TGCA'G
Cuts at: 0 623 905 992 1130 1289 1457 1607 1661
Size: 623 282 87 138 159 168 150 54
Cuts at: 1661 1673 2709
Size: 12 1036
Fragments arranged by size:
1036 623 282 168 159 150 138 87
54 12
PvuII CAG'CTG
Cuts at: 0 13 165 1290 1338 1362 1452 1623 1698
Size: 13 152 1125 48 24 90 171 75
Cuts at: 1698 1993 2115 2409 2709
Size: 295 122 294 300
Fragments arranged by size:
1125 300 295 294 171 152 122 90
75 48 24 13
SacI G_AGCT'C
Cuts at: 0 806 1568 2709
Size: 806 762 1141
Fragments arranged by size:
1141 806 762
SalI G'TCGA_C
Cuts at: 0 2185 2709
Size: 2185 524
SanDI GG'GwC_CC
Cuts at: 0 2316 2709
Size: 2316 393
SapI GCTCTTCn'nnn_
Cuts at: 0 989 1162 2709
Size: 989 173 1547
Fragments arranged by size:
1547 989 173
SbfI CC_TGCA'GG
Cuts at: 0 992 2709
Size: 992 1717
SfcI C'TryA_G
Cuts at: 0 619 640 901 988 1126 1285 1453 1603
Size: 619 21 261 87 138 159 168 150
Cuts at: 1603 1657 1669 2618 2709
Size: 54 12 949 91
Fragments arranged by size:
949 619 261 168 159 150 138 91
87 54 21 12
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 868 2709
Size: 868 1841
SmaI CCC'GGG
Cuts at: 0 1579 1761 2709
Size: 1579 182 948
Fragments arranged by size:
1579 948 182
SmlI C'TyrA_G
Cuts at: 0 1105 1730 2125 2243 2709
Size: 1105 625 395 118 466
Fragments arranged by size:
1105 625 466 395 118
StyI C'CwwG_G
Cuts at: 0 863 1811 2294 2401 2709
Size: 863 948 483 107 308
Fragments arranged by size:
948 863 483 308 107
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2554 2709
Size: 2554 155
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2252 2284 2643 2675 2709
Size: 2252 32 359 32 34
Fragments arranged by size:
2252 359 34 32 32
XmaI C'CCGG_G
Cuts at: 0 1577 1759 2709
Size: 1577 182 950
Fragments arranged by size:
1577 950 182
XmnI GAAnn'nnTTC
Cuts at: 0 302 2709
Size: 302 2407
Enzymes that do cut:
AarI AccI AcuI AflII AlfI AloI AlwNI ApaI
ApoI AvaI BamHI BanI BanII BbvCI BciVI BfrBI
BglI BglII BlpI Bme1580I BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI
BsrFI BstAPI BstXI BstYI Bsu36I BtgI BtsI EaeI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII
Hin4I HincII HindIII MmeI MscI MslI MspA1I NaeI
NcoI NgoMIV NheI NsiI PasI PflMI PfoI PmlI
PpiI PpuMI PspOMI PstI PvuII SacI SalI SanDI
SapI SbfI SfcI SfiI SmaI SmlI StyI TaqII
TstI XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflIII AgeI AhdI AleI
ApaLI AscI AseI AsiSI AvrII BaeI BbeI BbsI
BcgI BclI BmgBI BsaBI BsaHI BsaWI BsiWI BspEI
BsrDI BsrGI BssHII BssSI BstBI BstEII BstZ17I BtgZI
ClaI CspCI DraI DraIII DrdI EagI EcoRI EcoRV
FseI FspI FspAI HpaI KasI KpnI MfeI MluI
NarI NdeI NotI NruI NspI PacI PciI PmeI
PshAI PsiI PspXI PsrI PvuI RsrII SacII ScaI
SexAI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI
SspI StuI SwaI TatI Tth111I XbaI XcmI XhoI
ZraI