(Circular) (Six-base) MAPSORT of: pFN21AB8485.seq Check: 8168 from: 1 to: 7474 pFN21AB8485 7474 bp With 182 enzymes: SgfI * April 15, 2010 09:29 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5284 278 Size: 53 83 186 936 3748 2468 Fragments arranged by size: 3748 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2733 4817 1937 Size: 796 2084 4594 Fragments arranged by size: 4594 2084 796 Acc65I G'GTAC_C Cuts at: 4795 4795 Size: 7474 AclI AA'CG_TT Cuts at: 1563 5601 5974 1563 Size: 4038 373 3063 Fragments arranged by size: 4038 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2007 2482 2818 4334 5537 6921 7189 1632 Size: 375 475 336 1516 1203 1384 268 1917 Fragments arranged by size: 1917 1516 1384 1203 475 375 336 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7277 AflIII A'CryG_T Cuts at: 1203 2748 3055 3664 6373 7350 1203 Size: 1545 307 609 2709 977 1327 Fragments arranged by size: 2709 1545 1327 977 609 307 AgeI A'CCGG_T Cuts at: 6305 6305 Size: 7474 AhdI GACnn_n'nnGTC Cuts at: 2893 4216 6203 2893 Size: 1323 1987 4164 Fragments arranged by size: 4164 1987 1323 AleI CACnn'nnGTG Cuts at: 1166 3165 3677 1166 Size: 1999 512 4963 Fragments arranged by size: 4963 1999 512 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3183 3338 3919 1184 Size: 1999 155 581 4739 Fragments arranged by size: 4739 1999 581 155 AlwNI CAG_nnn'CTG Cuts at: 2188 2485 2608 3312 3793 4592 5217 6789 2188 Size: 297 123 704 481 799 625 1572 2873 Fragments arranged by size: 2873 1572 799 704 625 481 297 123 ApaI G_GGCC'C Cuts at: 2082 2082 Size: 7474 ApaLI G'TGCA_C Cuts at: 3178 5531 6687 3178 Size: 2353 1156 3965 Fragments arranged by size: 3965 2353 1156 ApoI r'AATT_y Cuts at: 1493 4783 5009 5161 7270 1493 Size: 3290 226 152 2109 1697 Fragments arranged by size: 3290 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 6028 160 Size: 872 4996 1606 Fragments arranged by size: 4996 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7474 AvaI C'yCGr_G Cuts at: 1870 1943 2075 3632 3710 4799 1870 Size: 73 132 1557 78 1089 4545 Fragments arranged by size: 4545 1557 1089 132 78 73 BamHI G'GATC_C Cuts at: 4804 5171 4804 Size: 367 7107 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2678 2741 3300 3444 4064 Size: 325 217 627 891 63 559 144 620 Cuts at: 4064 4151 4795 6250 618 Size: 87 644 1455 1842 Fragments arranged by size: 1842 1455 891 644 627 620 559 325 217 144 87 63 BanII G_rGCy'C Cuts at: 1391 2082 2364 2831 3131 3540 4092 4793 1391 Size: 691 282 467 300 409 552 701 4072 Fragments arranged by size: 4072 701 691 552 467 409 300 282 BbeI G_GCGC'C Cuts at: 3448 4155 3448 Size: 707 6767 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2013 2799 928 Size: 971 114 786 5603 Fragments arranged by size: 5603 971 786 114 BbvCI CC'TCA_GC Cuts at: 2189 2189 Size: 7474 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2636 2670 4748 4782 5665 5699 1903 Size: 34 699 34 2078 34 883 34 3678 Fragments arranged by size: 3678 2078 883 699 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5366 6576 5366 Size: 1210 6264 BclI T'GATC_A Cuts at: 1548 3487 4254 1548 Size: 1939 767 4768 Fragments arranged by size: 4768 1939 767 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2303 3212 3431 Size: 107 122 71 631 605 631 909 219 Cuts at: 3431 6085 136 Size: 2654 1525 Fragments arranged by size: 2654 1525 909 631 631 605 219 122 107 71 BglII A'GATC_T Cuts at: 3696 7469 3696 Size: 3773 3701 BlpI GC'TnA_GC Cuts at: 3029 4184 4891 3029 Size: 1155 707 5612 Fragments arranged by size: 5612 1155 707 Bme1580I G_kGCm'C Cuts at: 1790 2082 2140 2681 2744 3182 3641 5535 6691 Size: 292 58 541 63 438 459 1894 1156 Cuts at: 6691 1790 Size: 2573 Fragments arranged by size: 2573 1894 1156 541 459 438 292 63 58 BmgBI CAC'GTC Cuts at: 1318 2650 3163 1318 Size: 1332 513 5629 Fragments arranged by size: 5629 1332 513 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3093 3824 5205 Size: 416 53 83 399 24 1676 731 1381 Cuts at: 5205 6163 442 Size: 958 1753 Fragments arranged by size: 1753 1676 1381 958 731 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7474 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2845 3094 3571 3718 2845 Size: 249 477 147 6601 Fragments arranged by size: 6601 477 249 147 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2306 3218 3245 3473 4621 6134 1239 Size: 1067 912 27 228 1148 1513 2579 Fragments arranged by size: 2579 1513 1148 1067 912 228 27 Bpu10I CC'TnA_GC Cuts at: 2189 3125 3794 2189 Size: 936 669 5869 Fragments arranged by size: 5869 936 669 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5599 6464 6762 5599 Size: 865 298 6311 Fragments arranged by size: 6311 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3452 6137 882 Size: 617 1953 2685 2219 Fragments arranged by size: 2685 2219 1953 617 BsaAI yAC'GTr Cuts at: 493 1204 2239 3058 3665 5265 493 Size: 711 1035 819 607 1600 2702 Fragments arranged by size: 2702 1600 1035 819 711 607 BsaBI GATnn'nnATC Cuts at: 7468 7468 Size: 7474 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3445 4152 4217 4467 Size: 53 83 186 936 1912 707 65 250 Cuts at: 4467 5281 5663 6298 275 Size: 814 382 635 1451 Fragments arranged by size: 1912 1451 936 814 707 635 382 250 186 83 65 53 BsaWI w'CCGG_w Cuts at: 2220 3278 3474 4092 5907 6305 6579 6726 7137 Size: 1058 196 618 1815 398 274 147 411 Cuts at: 7137 2220 Size: 2557 Fragments arranged by size: 2557 1815 1058 618 411 398 274 196 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2262 2292 3243 3273 3303 Size: 30 165 30 859 30 951 30 30 Cuts at: 3303 1178 Size: 5349 Fragments arranged by size: 5349 951 859 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2078 2777 3470 3544 4100 1186 Size: 892 699 693 74 556 4560 Fragments arranged by size: 4560 892 699 693 556 74 BseYI C'CCAG_C Cuts at: 2290 2401 2851 3192 4515 6677 7173 2290 Size: 111 450 341 1323 2162 496 2591 Fragments arranged by size: 2591 2162 1323 496 450 341 111 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2123 2593 2995 3176 3701 4607 1163 Size: 573 387 470 402 181 525 906 4030 Fragments arranged by size: 4030 906 573 525 470 402 387 181 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3174 4924 5685 6713 1540 Size: 275 186 1173 1750 761 1028 2301 Fragments arranged by size: 2301 1750 1173 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2543 2914 3182 4092 5535 5620 6691 2543 Size: 371 268 910 1443 85 1071 3326 Fragments arranged by size: 3326 1443 1071 910 371 268 85 BsmI GAATG_Cn' Cuts at: 3359 4985 5078 3359 Size: 1626 93 5755 Fragments arranged by size: 5755 1626 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2082 2140 2364 2543 2681 2744 2831 Size: 399 292 58 224 179 138 63 87 Cuts at: 2831 2914 3131 3182 3540 3641 4092 4793 5535 Size: 83 217 51 358 101 451 701 742 Cuts at: 5535 5620 6691 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 701 451 399 358 292 224 217 179 138 101 87 85 83 63 58 51 BspEI T'CCGG_A Cuts at: 2220 4092 7137 2220 Size: 1872 3045 2557 Fragments arranged by size: 3045 2557 1872 BspHI T'CATG_A Cuts at: 5363 7093 5363 Size: 1730 5744 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4408 4829 844 Size: 3564 421 3489 Fragments arranged by size: 3564 3489 421 BsrBI CCG'CTC Cuts at: 1394 1823 3298 3798 5361 1394 Size: 429 1475 500 1563 3507 Fragments arranged by size: 3507 1563 1475 500 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5969 6143 65 Size: 1183 4721 174 1396 Fragments arranged by size: 4721 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3441 4061 6118 6305 1700 Size: 110 1631 620 2057 187 2869 Fragments arranged by size: 2869 2057 1631 620 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7474 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7474 BssSI C'ACGA_G Cuts at: 4767 5534 6546 4767 Size: 767 1012 5695 Fragments arranged by size: 5695 1012 767 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7474 BstBI TT'CG_AA Cuts at: 6278 6278 Size: 7474 BstEII G'GTnAC_C Cuts at: 1184 4732 1184 Size: 3548 3926 BstXI CCAn_nnnn'nTGG Cuts at: 2167 3731 4523 4645 2167 Size: 1564 792 122 4996 Fragments arranged by size: 4996 1564 792 122 BstYI r'GATC_y Cuts at: 1972 2151 3696 4338 4438 4554 4804 5171 5556 Size: 179 1545 642 100 116 250 367 385 Cuts at: 5556 5573 7025 7111 7123 7469 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1545 1452 642 385 367 346 250 179 116 100 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2175 3417 3525 4542 513 Size: 552 939 171 1242 108 1017 3445 Fragments arranged by size: 3445 1242 1017 939 552 171 108 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2853 3372 3419 487 Size: 683 56 1627 519 47 4542 Fragments arranged by size: 4542 1627 683 519 56 47 BtsI GCAGTG_nn' Cuts at: 2309 2437 2581 2787 3966 4593 4990 5784 5804 Size: 128 144 206 1179 627 397 794 20 Cuts at: 5804 2309 Size: 3979 Fragments arranged by size: 3979 1179 794 627 397 206 144 128 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7474 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7439 DraI TTT'AAA Cuts at: 4778 4778 Size: 7474 DraIII CAC_nnn'GTG Cuts at: 2315 3757 2315 Size: 1442 6032 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4009 6481 809 Size: 507 2693 2472 1802 Fragments arranged by size: 2693 2472 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2410 3171 3213 Size: 54 1425 50 275 18 580 761 42 Cuts at: 3213 3273 3391 3528 4176 4650 4921 5810 7439 Size: 60 118 137 648 474 271 889 1629 Cuts at: 7439 8 Size: 43 Fragments arranged by size: 1629 1425 889 761 648 580 474 275 271 137 118 60 54 50 43 42 18 EagI C'GGCC_G Cuts at: 1537 1812 3171 4921 1537 Size: 275 1359 1750 4090 Fragments arranged by size: 4090 1750 1359 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4328 4572 5404 1353 Size: 2975 244 832 3423 Fragments arranged by size: 3423 2975 832 244 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4274 6061 6435 6581 387 Size: 1064 138 2685 1787 374 146 1280 Fragments arranged by size: 2685 1787 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 4090 4090 Size: 7474 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2007 2306 2482 2818 3218 3245 3473 Size: 393 375 299 176 336 400 27 228 Cuts at: 3473 4334 4621 5537 6134 6921 7189 1239 Size: 861 287 916 597 787 268 1524 Fragments arranged by size: 1524 916 861 787 597 400 393 375 336 299 287 268 228 176 27 EcoNI CCTnn'n_nnAGG Cuts at: 3452 3857 4415 3452 Size: 405 558 6511 Fragments arranged by size: 6511 558 405 EcoO109I rG'GnC_Cy Cuts at: 2079 2304 2634 2995 3073 3790 4027 4570 2079 Size: 225 330 361 78 717 237 543 4983 Fragments arranged by size: 4983 717 543 361 330 237 225 78 EcoRI G'AATT_C Cuts at: 4783 5161 4783 Size: 378 7096 FspI TGC'GCA Cuts at: 2463 4758 5980 2463 Size: 2295 1222 3957 Fragments arranged by size: 3957 2295 1222 HaeII r_GCGC'y Cuts at: 1678 2189 2852 3448 4155 4348 6621 1678 Size: 511 663 596 707 193 2273 2531 Fragments arranged by size: 2531 2273 707 663 596 511 193 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2879 2911 4154 4186 4202 Size: 32 135 32 1307 32 1243 32 16 Cuts at: 4202 4234 4694 4726 6115 6147 6189 6221 1373 Size: 32 460 32 1389 32 42 32 2626 Fragments arranged by size: 2626 1389 1307 1243 460 135 42 32 32 32 32 32 32 32 32 16 HincII GTy'rAC Cuts at: 669 1657 1938 4764 4818 5064 669 Size: 988 281 2826 54 246 3079 Fragments arranged by size: 3079 2826 988 281 246 54 HindIII A'AGCT_T Cuts at: 748 748 Size: 7474 HpaI GTT'AAC Cuts at: 5064 5064 Size: 7474 KasI G'GCGC_C Cuts at: 3444 4151 3444 Size: 707 6767 KpnI G_GTAC'C Cuts at: 4799 4799 Size: 7474 MfeI C'AATT_G Cuts at: 5073 5073 Size: 7474 MluI A'CGCG_T Cuts at: 2748 7350 2748 Size: 4602 2872 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4036 6588 6772 1305 Size: 2731 2552 184 2007 Fragments arranged by size: 2731 2552 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2412 3275 3530 4178 10 Size: 54 1425 343 580 863 255 648 3306 Fragments arranged by size: 3306 1425 863 648 580 343 255 54 MslI CAynn'nnrTG Cuts at: 518 1166 2648 2684 3165 3677 4139 5432 5791 Size: 648 1482 36 481 512 462 1293 359 Cuts at: 5791 5950 518 Size: 159 2042 Fragments arranged by size: 2042 1482 1293 648 512 481 462 359 159 36 MspA1I CmG'CkG Cuts at: 1606 2605 3218 4260 4739 4890 5567 6715 6960 Size: 999 613 1042 479 151 677 1148 245 Cuts at: 6960 1606 Size: 2120 Fragments arranged by size: 2120 1148 1042 999 677 613 479 245 151 NarI GG'CG_CC Cuts at: 3445 4152 3445 Size: 707 6767 NcoI C'CATG_G Cuts at: 513 1065 2004 2175 3525 513 Size: 552 939 171 1350 4462 Fragments arranged by size: 4462 1350 939 552 171 NdeI CA'TA_TG Cuts at: 387 387 Size: 7474 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7474 NotI GC'GGCC_GC Cuts at: 4921 4921 Size: 7474 NspI r_CATG'y Cuts at: 1132 1229 2777 3568 4132 4267 4832 6377 1132 Size: 97 1548 791 564 135 565 1545 2229 Fragments arranged by size: 2229 1548 1545 791 565 564 135 97 PasI CC'CwG_GG Cuts at: 1348 3131 3724 1348 Size: 1783 593 5098 Fragments arranged by size: 5098 1783 593 PciI A'CATG_T Cuts at: 6373 6373 Size: 7474 PflMI CCAn_nnn'nTGG Cuts at: 1108 2596 2884 3757 1108 Size: 1488 288 873 4825 Fragments arranged by size: 4825 1488 873 288 PfoI T'CCnGG_A Cuts at: 2454 2552 3048 4557 2454 Size: 98 496 1509 5371 Fragments arranged by size: 5371 1509 496 98 PmeI GTTT'AAAC Cuts at: 4778 4778 Size: 7474 PmlI CAC'GTG Cuts at: 3058 3058 Size: 7474 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5543 5575 7056 7088 5543 Size: 32 1481 32 5929 Fragments arranged by size: 5929 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2995 3073 4027 2995 Size: 78 954 6442 Fragments arranged by size: 6442 954 78 PshAI GACnn'nnGTC Cuts at: 1613 4769 1613 Size: 3156 4318 PsiI TTA'TAA Cuts at: 5044 5044 Size: 7474 PspOMI G'GGCC_C Cuts at: 2078 2078 Size: 7474 PstI C_TGCA'G Cuts at: 830 2118 2490 3114 3317 3327 4698 4826 830 Size: 1288 372 624 203 10 1371 128 3478 Fragments arranged by size: 3478 1371 1288 624 372 203 128 10 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7474 PvuII CAG'CTG Cuts at: 2605 2605 Size: 7474 RsrII CG'GwC_CG Cuts at: 2218 2218 Size: 7474 SacI G_AGCT'C Cuts at: 4092 4092 Size: 7474 SalI G'TCGA_C Cuts at: 1936 4816 1936 Size: 2880 4594 SapI GCTCTTCn'nnn_ Cuts at: 4572 4572 Size: 7474 SbfI CC_TGCA'GG Cuts at: 3114 3327 4826 3114 Size: 213 1499 5762 Fragments arranged by size: 5762 1499 213 ScaI AGT'ACT Cuts at: 5722 7434 5722 Size: 1712 5762 SfcI C'TryA_G Cuts at: 826 1045 2114 2144 2486 3110 3313 3323 4694 Size: 219 1069 30 342 624 203 10 1371 Cuts at: 4694 4822 5957 6638 6829 826 Size: 128 1135 681 191 1471 Fragments arranged by size: 1471 1371 1135 1069 681 624 342 219 203 191 128 30 10 SfiI GGCCn_nnn'nGGCC Cuts at: 2303 2303 Size: 7474 SfoI GGC'GCC Cuts at: 3446 4153 3446 Size: 707 6767 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7474 SgrAI Cr'CCGG_yG Cuts at: 1700 3441 1700 Size: 1741 5733 SmaI CCC'GGG Cuts at: 1872 2077 4801 1872 Size: 205 2724 4545 Fragments arranged by size: 4545 2724 205 SmlI C'TyrA_G Cuts at: 820 1017 1943 5578 6479 6741 820 Size: 197 926 3635 901 262 1553 Fragments arranged by size: 3635 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 5265 493 Size: 4772 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7474 SphI G_CATG'C Cuts at: 1132 3568 4832 1132 Size: 2436 1264 3774 Fragments arranged by size: 3774 2436 1264 SrfI GCCC'GGGC Cuts at: 2077 2077 Size: 7474 SspI AAT'ATT Cuts at: 5 52 5398 5 Size: 47 5346 2081 Fragments arranged by size: 5346 2081 47 StuI AGG'CCT Cuts at: 1517 2940 3551 4047 1517 Size: 1423 611 496 4944 Fragments arranged by size: 4944 1423 611 496 StyI C'CwwG_G Cuts at: 513 1065 2004 2175 3034 3525 3531 3882 4312 Size: 552 939 171 859 491 6 351 430 Cuts at: 4312 4913 513 Size: 601 3074 Fragments arranged by size: 3074 939 859 601 552 491 430 351 171 6 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2314 2393 2939 5501 5518 5671 5856 Size: 1255 734 79 546 2562 17 153 185 Cuts at: 5856 6301 325 Size: 445 1498 Fragments arranged by size: 2562 1498 1255 734 546 445 185 153 79 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5720 7432 96 Size: 274 80 33 51 1443 3743 1712 138 Fragments arranged by size: 3743 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2262 2294 3273 3305 7434 7466 2262 Size: 32 979 32 4129 32 2270 Fragments arranged by size: 4129 2270 979 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7474 XbaI T'CTAG_A Cuts at: 4810 4810 Size: 7474 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7474 XmaI C'CCGG_G Cuts at: 1870 2075 4799 1870 Size: 205 2724 4545 Fragments arranged by size: 4545 2724 205 XmnI GAAnn'nnTTC Cuts at: 5603 5603 Size: 7474 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5282 276 Size: 53 83 186 936 3748 2468 Fragments arranged by size: 3748 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BfrBI BsiWI BsmBI BstZ17I Bsu36I EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PspXI PsrI SacII SanDI SexAI SwaI XcmI