Restriction map for
pFN21AB5939
##################################
# NO CUT ENZYMES ( 121 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AgeI AocI Aor51HI AscI
AseI AsnI Asp700I Asp718I AviII
AvrII BamHI BanI BbeI BbrPI
BbvCI BcgI BciVI BfaI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaAI BsaI BsaWI BseAI BsgI
BsiWI BsmI Bsp1407I BspEI BspHI
BspLU11I BsrDI BsrFI BsrGI BssSI
Bst1107I BstBI BstZ17I Bsu36I CelII
Cfr10I CpoI DraI DraIII Eco47III
Eco81I EcoRI EcoRV EcoT22I FauI
FseI FspI HincII HindII HindIII
HpaI KasI KpnI KspI MaeI
MfeI MluI MroI MslI MunI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI XbaI XcmI XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AcsI 1402 AAATTC [GA]^AATT[CT]
AflIII 572 ACGTGT A^C[GA][CT]GT
AhdI 487 GACGTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1309 GTGCAC G^TGCAC
AlwNI 1072 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 399 GGGCCC GGGCC^C
ApaLI 1309 GTGCAC G^TGCAC
ApoI 1402 AAATTC [GA]^AATT[CT]
AspEI 487 GACGTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 1309 GTGCAC G[AT]GC[AT]^C
AspI 70 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanII 399 GGGCCC G[GA]GC[CT]^C
BclI 1497 TGATCA T^GATCA
BsaBI 1441 GATGACGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 938 CGGCCG CG[GA][CT]^CG
BsiHKAI 1309 GTGCAC G[AT]GC[AT]^C
BsmBI 1234 GAGACG CGTCTC(1/5)
BsmFI 1091 GGGAC GGGAC(10/14)
BspDI 535 ATCGAT AT^CGAT
BsrBI 1413 GAGCGG CCGCTC(-3/-3)
BssHII 45 GCGCGC G^CGCGC
BstAPI 510 GCAGTTTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 657 GGTCACC G^GT[ACGT]ACC
BstPI 657 GGTCACC G^GT[ACGT]ACC
BstYI 854 AGATCC [GA]^GATC[CT]
ClaI 535 ATCGAT AT^CGAT
DraII 1454 AGGACCT [GA]G^G[ACGT]CC[CT]
DrdI 1351 GACAGGACGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 938 CGGCCG C^GGCCG
Eam1105I 487 GACGTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 928 GAAGAG CTCTTC(1/4)
EclXI 938 CGGCCG C^GGCCG
Eco52I 938 CGGCCG C^GGCCG
EcoO109I 1454 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoO65I 657 GGTCACC G^GT[ACGT]ACC
FbaI 1497 TGATCA T^GATCA
HaeII 108 GGCGCT [GA]GCGC^[CT]
Ksp632I 928 GAAGAG CTCTTC(1/4)
MamI 1441 GATGACGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 854 AGATCC [GA]^GATC[CT]
NcoI 1183 CCATGG C^CATGG
PflFI 70 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 1454 AGGACCT [GA]G^G[AT]CC[CT]
PstI 434 CTGCAG CTGCA^G
SfaNI 899 GCATC GCATC(5/9)
SfcI 434 CTGCAG C^T[GA][CT]AG
TfiI 988 GAATC G^A[AT]TC
Tsp509I 1403 AATT ^AATT
Tth111I 70 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoII 854 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AcyI 52 GACGCC G[GA]^CG[CT]C
247 GACGCC
BbsI 1132 GAAGAC GAAGAC(2/6)
1489 GAAGAC
BcnI 402 CCCGG CC^[GC]GG
936 CCCGG
BpuAI 1132 GAAGAC GAAGAC(2/6)
1489 GAAGAC
BsaHI 52 GACGCC G[GA]^CG[CT]C
247 GACGCC
BsmAI 323 GAGAC GTCTC(1/5)
1234 GAGAC
BspMI 128 ACCTGC ACCTGC(4/8)
671 ACCTGC
BstXI 455 CCAGCGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1286 CCAACGAGGTGG
DdeI 182 CTGAG C^T[ACGT]AG
814 CTGAG
EcoNI 918 CCTGAAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
957 CCTGGAGGAGG
HgaI 52 GACGC GACGC(5/10)
247 GACGC
Hin1I 52 GACGCC G[GA]^CG[CT]C
247 GACGCC
MseI 617 TTAA T^TAA
1397 TTAA
NciI 402 CCCGG CC^[GC]GG
936 CCCGG
PaeR7I 904 CTCGAG C^TCGAG
1450 CTCGAG
PflMI 72 CCACGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1183 CCATGGTGTGG
Tru9I 617 TTAA T^TAA
1397 TTAA
Van91I 72 CCACGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1183 CCATGGTGTGG
XhoI 904 CTCGAG C^TCGAG
1450 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 68 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AvaI AvaII BalI BbvI BmyI
BpmI BsaJI BseRI BsiYI BslI
BsoBI Bsp1286I BsrI BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr13I
DpnII DsaI EaeI EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
MaeII MaeIII MboI MboII MluNI
MnlI MscI MspA1I MspI MvaI
MvnI MwoI NdeII NlaIII NlaIV
PleI PvuII RsaI Sau3AI Sau96I
ScrFI SmlI StyI TaqI TseI
Tsp45I TspRI TthHB8I