Restriction map for pFN21AB5929
 (Circular) (Six-base) MAPSORT of: pFN21AB5929.seq  Check: 7413  from: 1  to: 6226



pFN21AB5929  6226 bp

 With 182 enzymes: SgfI * 

                        April 15, 2010 09:29  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    4036     278 
   Size:         53      83     186     936    2500    2468

  Fragments arranged by size: 

                2500    2468     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    3569    1937 
   Size:       1632    4594
 
Acc65I G'GTAC_C

Cuts at:   2042    3547    2042 
   Size:       1505    4721
 
AclI AA'CG_TT

Cuts at:   1563    4353    4726    1563 
   Size:       2790     373    3063

  Fragments arranged by size: 

                3063    2790     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2299    3035    3090    3372    4289    5673    5941    1632 
   Size:        667     736      55     282     917    1384     268    1917

  Fragments arranged by size: 

                1917    1384     917     736     667     282     268      55
            
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    6029
 
AflIII A'CryG_T

Cuts at:   1203    5125    6102    1203 
   Size:       3922     977    1327

  Fragments arranged by size: 

                3922    1327     977
 
AgeI A'CCGG_T

Cuts at:   5057    5057 
   Size:       6226
 
AhdI GACnn_n'nnGTC

Cuts at:   4955    4955 
   Size:       6226
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       6226
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       6226
 
AlwNI CAG_nnn'CTG

Cuts at:   2114    3969    5541    2114 
   Size:       1855    1572    2799

  Fragments arranged by size: 

                2799    1855    1572
 
ApaLI G'TGCA_C

Cuts at:   4283    5439    4283 
   Size:       1156    5070
 
ApoI r'AATT_y

Cuts at:   1493    2492    3186    3535    3761    3913    6022    1493 
   Size:        999     694     349     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697     999     694     349     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    4780     160 
   Size:        872    3748    1606

  Fragments arranged by size: 

                3748    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       6226
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2029    3551    1870 
   Size:         73      86    1522    4545

  Fragments arranged by size: 

                4545    1522      86      73
 
BamHI G'GATC_C

Cuts at:   3556    3923    3556 
   Size:        367    5859
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2033    2042    3547    5002     618 
   Size:        325     217     627     246       9    1505    1455    1842

  Fragments arranged by size: 

                1842    1505    1455     627     325     246     217       9
            
 
BanII G_rGCy'C

Cuts at:   1391    2026    3545    1391 
   Size:        635    1519    4072

  Fragments arranged by size: 

                4072    1519     635
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    3016     928 
   Size:        971    1117    4138

  Fragments arranged by size: 

                4138    1117     971
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    4417    4451    1903 
   Size:         34    2480      34    3678

  Fragments arranged by size: 

                3678    2480      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3241    4118    5328    3241 
   Size:        877    1210    4139

  Fragments arranged by size: 

                4139    1210     877
 
BclI T'GATC_A

Cuts at:   1548    2710    1548 
   Size:       1162    5064
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    2060    3215    4837 
   Size:        107     122      71     631     605     388    1155    1622

Cuts at:   4837     136 
   Size:       1525

  Fragments arranged by size: 

                1622    1525    1155     631     605     388     122     107
                  71
 
BglII A'GATC_T

Cuts at:   3347    3376    6221    3347 
   Size:         29    2845    3352

  Fragments arranged by size: 

                3352    2845      29
 
BlpI GC'TnA_GC

Cuts at:   3643    3643 
   Size:       6226
 
Bme1580I G_kGCm'C

Cuts at:   1790    2036    2693    4287    5443    1790 
   Size:        246     657    1594    1156    2573

  Fragments arranged by size: 

                2573    1594    1156     657     246
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       6226
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    2267    2335    2544 
   Size:        416      53      83     399      24     850      68     209

Cuts at:   2544    3957    4915     442 
   Size:       1413     958    1753

  Fragments arranged by size: 

                1753    1413     958     850     416     399     209      83
                  68      53      24
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       6226
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    4886    1239 
   Size:       3647    2579
 
Bpu10I CC'TnA_GC

Cuts at:   3425    3425 
   Size:       6226
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   4351    5216    5514    4351 
   Size:        865     298    5063

  Fragments arranged by size: 

                5063     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    2315    4889     882 
   Size:        617     816    2574    2219

  Fragments arranged by size: 

                2574    2219     816     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    4017     493 
   Size:        711    2813    2702

  Fragments arranged by size: 

                2813    2702     711
 
BsaBI GATnn'nnATC

Cuts at:   3375    3440    6220    3375 
   Size:         65    2780    3381

  Fragments arranged by size: 

                3381    2780      65
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    4033    4415    5050     275 
   Size:         53      83     186     936    2500     382     635    1451

  Fragments arranged by size: 

                2500    1451     936     635     382     186      83      53
            
 
BsaWI w'CCGG_w

Cuts at:   2039    4659    5057    5331    5478    5889    2039 
   Size:       2620     398     274     147     411    2376

  Fragments arranged by size: 

                2620    2376     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    2317    2347    1178 
   Size:         30     165      30     914      30    5057

  Fragments arranged by size: 

                5057     914     165      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    1186 
   Size:       6226
 
BseYI C'CCAG_C

Cuts at:   5429    5925    5429 
   Size:        496    5730
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    2859    1163 
   Size:        573    1123    4530

  Fragments arranged by size: 

                4530    1123     573
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    3676    4437    5465    1540 
   Size:        275     186    1675     761    1028    2301

  Fragments arranged by size: 

                2301    1675    1028     761     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   2374    4287    4372    5443    2374 
   Size:       1913      85    1071    3157

  Fragments arranged by size: 

                3157    1913    1071      85
 
BsmI GAATG_Cn'

Cuts at:   2117    3737    3830    2117 
   Size:       1620      93    4513

  Fragments arranged by size: 

                4513    1620      93
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2026    2036    2374    2693    3545    4287    4372 
   Size:        399     236      10     338     319     852     742      85

Cuts at:   4372    5443    1391 
   Size:       1071    2174

  Fragments arranged by size: 

                2174    1071     852     742     399     338     319     236
                  85      10
 
BspEI T'CCGG_A

Cuts at:   5889    5889 
   Size:       6226
 
BspHI T'CATG_A

Cuts at:   4115    5845    4115 
   Size:       1730    4496
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2099    3581     844 
   Size:       1255    1482    3489

  Fragments arranged by size: 

                3489    1482    1255
 
BsrBI CCG'CTC

Cuts at:   1394    1823    4113    1394 
   Size:        429    2290    3507

  Fragments arranged by size: 

                3507    2290     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    2639    2699    4721    4895      65 
   Size:       1183    1391      60    2022     174    1396

  Fragments arranged by size: 

                2022    1396    1391    1183     174      60
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    4870    5057    1700 
   Size:        110    3060     187    2869

  Fragments arranged by size: 

                3060    2869     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       6226
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       6226
 
BssSI C'ACGA_G

Cuts at:   4286    5298    4286 
   Size:       1012    5214
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794    2114     794 
   Size:       1320    4906
 
BstBI TT'CG_AA

Cuts at:   2611    5030    2611 
   Size:       2419    3807
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       6226
 
BstYI r'GATC_y

Cuts at:   1972    3145    3347    3376    3556    3923    4308    4325    5777 
   Size:       1173     202      29     180     367     385      17    1452

Cuts at:   5777    5863    5875    6221    1972 
   Size:         86      12     346    1977

  Fragments arranged by size: 

                1977    1452    1173     385     367     346     202     180
                  86      29      17      12
 
BtgI C'CryG_G

Cuts at:    513    1065    2004    2058    2067     513 
   Size:        552     939      54       9    4672

  Fragments arranged by size: 

                4672     939     552      54       9
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    5487

  Fragments arranged by size: 

                5487     683      56
 
BtsI GCAGTG_nn'

Cuts at:   3742    4536    4556    3742 
   Size:        794      20    5412

  Fragments arranged by size: 

                5412     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       6226
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    6191
 
DraI TTT'AAA

Cuts at:   3530    3530 
   Size:       6226
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    5233     809 
   Size:        507    3917    1802

  Fragments arranged by size: 

                3917    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    3673    4562    6191 
   Size:         54    1425      50     275      18    1843     889    1629

Cuts at:   6191       8 
   Size:         43

  Fragments arranged by size: 

                1843    1629    1425     889     275      54      50      43
                  18
 
EagI C'GGCC_G

Cuts at:   1537    1812    3673    1537 
   Size:        275    1861    4090

  Fragments arranged by size: 

                4090    1861     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2318    2761    3191    3219    4156    1353 
   Size:        965     443     430      28     937    3423

  Fragments arranged by size: 

                3423     965     937     443     430      28
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    2472    4813    5187    5333     387 
   Size:       1064     138     883    2341     374     146    1280

  Fragments arranged by size: 

                2341    1280    1064     883     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2299    3035    3090    3372    4289    4886    5673 
   Size:        393     667     736      55     282     917     597     787

Cuts at:   5673    5941    1239 
   Size:        268    1524

  Fragments arranged by size: 

                1524     917     787     736     667     597     393     282
                 268      55
 
EcoO109I rG'GnC_Cy

Cuts at:   3169    3169 
   Size:       6226
 
EcoRI G'AATT_C

Cuts at:   2492    3535    3913    2492 
   Size:       1043     378    4805

  Fragments arranged by size: 

                4805    1043     378
 
EcoRV GAT'ATC

Cuts at:   3061    3061 
   Size:       6226
 
FspI TGC'GCA

Cuts at:   4732    4732 
   Size:       6226
 
HaeII r_GCGC'y

Cuts at:   1678    5373    1678 
   Size:       3695    2531
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    3045    3050    3077    3082    3313 
   Size:         32     135      32    1473       5      27       5     231

Cuts at:   3313    3345    3501    3533    4867    4899    4941    4973    1373 
   Size:         32     156      32    1334      32      42      32    2626

  Fragments arranged by size: 

                2626    1473    1334     231     156     135      42      32
                  32      32      32      32      32      27       5       5
            
 
HincII GTy'rAC

Cuts at:    669    1657    1938    3570    3816     669 
   Size:        988     281    1632     246    3079

  Fragments arranged by size: 

                3079    1632     988     281     246
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       6226
 
HpaI GTT'AAC

Cuts at:   3816    3816 
   Size:       6226
 
KpnI G_GTAC'C

Cuts at:   2046    3551    2046 
   Size:       1505    4721
 
MfeI C'AATT_G

Cuts at:   2822    3825    2822 
   Size:       1003    5223
 
MluI A'CGCG_T

Cuts at:   6102    6102 
   Size:       6226
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    2370    2408    5340    5524    1305 
   Size:       1065      38    2932     184    2007

  Fragments arranged by size: 

                2932    2007    1065     184      38
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832      10 
   Size:         54    1425     343    4404

  Fragments arranged by size: 

                4404    1425     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    4184    4543    4702     518 
   Size:        648    3018     359     159    2042

  Fragments arranged by size: 

                3018    2042     648     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    2048    2060    2069    2353    3244    3642    4319    5467 
   Size:        442      12       9     284     891     398     677    1148

Cuts at:   5467    5712    1606 
   Size:        245    2120

  Fragments arranged by size: 

                2120    1148     891     677     442     398     284     245
                  12       9
 
NcoI C'CATG_G

Cuts at:    513    1065    2004     513 
   Size:        552     939    4735

  Fragments arranged by size: 

                4735     939     552
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       6226
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       6226
 
NotI GC'GGCC_GC

Cuts at:   3673    3673 
   Size:       6226
 
NspI r_CATG'y

Cuts at:   1132    1229    2548    3584    5129    1132 
   Size:         97    1319    1036    1545    2229

  Fragments arranged by size: 

                2229    1545    1319    1036      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       6226
 
PciI A'CATG_T

Cuts at:   5125    5125 
   Size:       6226
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    1108 
   Size:       6226
 
PmeI GTTT'AAAC

Cuts at:   3530    3530 
   Size:       6226
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   4295    4327    5808    5840    4295 
   Size:         32    1481      32    4681

  Fragments arranged by size: 

                4681    1481      32      32
 
PpuMI rG'GwC_Cy

Cuts at:   3169    3169 
   Size:       6226
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       6226
 
PsiI TTA'TAA

Cuts at:   3796    3796 
   Size:       6226
 
PstI C_TGCA'G

Cuts at:    830    2053    2110    2439    3578     830 
   Size:       1223      57     329    1139    3478

  Fragments arranged by size: 

                3478    1223    1139     329      57
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       6226
 
PvuII CAG'CTG

Cuts at:   2353    3244    2353 
   Size:        891    5335
 
SacII CC_GC'GG

Cuts at:   2061    2070    2061 
   Size:          9    6217
 
SalI G'TCGA_C

Cuts at:   1936    3568    1936 
   Size:       1632    4594
 
SapI GCTCTTCn'nnn_

Cuts at:   3219    3219 
   Size:       6226
 
SbfI CC_TGCA'GG

Cuts at:   3578    3578 
   Size:       6226
 
ScaI AGT'ACT

Cuts at:   2227    4474    6186    2227 
   Size:       2247    1712    2267

  Fragments arranged by size: 

                2267    2247    1712
 
SfcI C'TryA_G

Cuts at:    826    1045    2049    2106    2435    3108    3574    4709    5390 
   Size:        219    1004      57     329     673     466    1135     681

Cuts at:   5390    5581     826 
   Size:        191    1471

  Fragments arranged by size: 

                1471    1135    1004     681     673     466     329     219
                 191      57
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       6226
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       6226
 
SmaI CCC'GGG

Cuts at:   1872    3553    1872 
   Size:       1681    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    4330    5231    5493     820 
   Size:        197     926    2387     901     262    1553

  Fragments arranged by size: 

                2387    1553     926     901     262     197
 
SnaBI TAC'GTA

Cuts at:    493    4017     493 
   Size:       3524    2702
 
SpeI A'CTAG_T

Cuts at:    152    2222     152 
   Size:       2070    4156
 
SphI G_CATG'C

Cuts at:   1132    2548    3584    1132 
   Size:       1416    1036    3774

  Fragments arranged by size: 

                3774    1416    1036
 
SspI AAT'ATT

Cuts at:      5      52    2479    4150       5 
   Size:         47    2427    1671    2081

  Fragments arranged by size: 

                2427    2081    1671      47
 
StuI AGG'CCT

Cuts at:   1517    2441    3423    1517 
   Size:        924     982    4320

  Fragments arranged by size: 

                4320     982     924
 
StyI C'CwwG_G

Cuts at:    513    1065    2004    2940    3665     513 
   Size:        552     939     936     725    3074

  Fragments arranged by size: 

                3074     939     936     725     552
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    4253    4270    4423    4608    5053     325 
   Size:       1255    2673      17     153     185     445    1498

  Fragments arranged by size: 

                2673    1498    1255     445     185     153      17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    2225    4472    6184 
   Size:        274      80      33      51    1443     248    2247    1712

Cuts at:   6184      96 
   Size:        138

  Fragments arranged by size: 

                2247    1712    1443     274     248     138      80      51
                  33
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   6186    6218    6186 
   Size:         32    6194
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       6226
 
XbaI T'CTAG_A

Cuts at:   3457    3562    3457 
   Size:        105    6121
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   3509    3509 
   Size:       6226
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       6226
 
XmaI C'CCGG_G

Cuts at:   1870    3551    1870 
   Size:       1681    4545
 
XmnI GAAnn'nnTTC

Cuts at:   4355    4355 
   Size:       6226
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    4034     276 
   Size:         53      83     186     936    2500    2468

  Fragments arranged by size: 

                2500    2468     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI    AsiSI
     AvaI    BamHI     BanI    BanII     BbsI     BcgI    BciVI     BclI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BpmI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstYI     BtgI    BtgZI     BtsI     ClaI
    CspCI     DraI     DrdI     EaeI     EagI     EarI     EciI  Eco57MI
 EcoO109I    EcoRI    EcoRV     FspI    HaeII    Hin4I   HincII  HindIII
     HpaI     KpnI     MfeI     MluI     MmeI     MscI     MslI   MspA1I
     NcoI     NdeI     NheI     NotI     NspI     PasI     PciI    PflMI
     PmeI     PpiI    PpuMI    PshAI     PsiI     PstI     PvuI    PvuII
    SacII     SalI     SapI     SbfI     ScaI     SfcI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SspI     StuI     StyI
    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI
     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     AloI     ApaI     AscI    AvrII     BaeI     BbeI
    BbvCI    BfrBI     BplI    BsiWI    BsmBI    BstXI  BstZ17I   Bsu36I
   DraIII  EcoICRI    EcoNI     FalI     FseI    FspAI     KasI     NaeI
     NarI   NgoMIV     NruI     NsiI     PacI     PfoI     PmlI   PspOMI
    PspXI     PsrI    RsrII     SacI    SanDI    SexAI     SfiI     SfoI
     SrfI     SwaI
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