(Circular) (Six-base) MAPSORT of: pFN21AB5929.seq Check: 7413 from: 1 to: 6226 pFN21AB5929 6226 bp With 182 enzymes: SgfI * April 15, 2010 09:29 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4036 278 Size: 53 83 186 936 2500 2468 Fragments arranged by size: 2500 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3569 1937 Size: 1632 4594 Acc65I G'GTAC_C Cuts at: 2042 3547 2042 Size: 1505 4721 AclI AA'CG_TT Cuts at: 1563 4353 4726 1563 Size: 2790 373 3063 Fragments arranged by size: 3063 2790 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2299 3035 3090 3372 4289 5673 5941 1632 Size: 667 736 55 282 917 1384 268 1917 Fragments arranged by size: 1917 1384 917 736 667 282 268 55 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6029 AflIII A'CryG_T Cuts at: 1203 5125 6102 1203 Size: 3922 977 1327 Fragments arranged by size: 3922 1327 977 AgeI A'CCGG_T Cuts at: 5057 5057 Size: 6226 AhdI GACnn_n'nnGTC Cuts at: 4955 4955 Size: 6226 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6226 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6226 AlwNI CAG_nnn'CTG Cuts at: 2114 3969 5541 2114 Size: 1855 1572 2799 Fragments arranged by size: 2799 1855 1572 ApaLI G'TGCA_C Cuts at: 4283 5439 4283 Size: 1156 5070 ApoI r'AATT_y Cuts at: 1493 2492 3186 3535 3761 3913 6022 1493 Size: 999 694 349 226 152 2109 1697 Fragments arranged by size: 2109 1697 999 694 349 226 152 AseI AT'TA_AT Cuts at: 160 1032 4780 160 Size: 872 3748 1606 Fragments arranged by size: 3748 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6226 AvaI C'yCGr_G Cuts at: 1870 1943 2029 3551 1870 Size: 73 86 1522 4545 Fragments arranged by size: 4545 1522 86 73 BamHI G'GATC_C Cuts at: 3556 3923 3556 Size: 367 5859 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2033 2042 3547 5002 618 Size: 325 217 627 246 9 1505 1455 1842 Fragments arranged by size: 1842 1505 1455 627 325 246 217 9 BanII G_rGCy'C Cuts at: 1391 2026 3545 1391 Size: 635 1519 4072 Fragments arranged by size: 4072 1519 635 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3016 928 Size: 971 1117 4138 Fragments arranged by size: 4138 1117 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4417 4451 1903 Size: 34 2480 34 3678 Fragments arranged by size: 3678 2480 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3241 4118 5328 3241 Size: 877 1210 4139 Fragments arranged by size: 4139 1210 877 BclI T'GATC_A Cuts at: 1548 2710 1548 Size: 1162 5064 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2060 3215 4837 Size: 107 122 71 631 605 388 1155 1622 Cuts at: 4837 136 Size: 1525 Fragments arranged by size: 1622 1525 1155 631 605 388 122 107 71 BglII A'GATC_T Cuts at: 3347 3376 6221 3347 Size: 29 2845 3352 Fragments arranged by size: 3352 2845 29 BlpI GC'TnA_GC Cuts at: 3643 3643 Size: 6226 Bme1580I G_kGCm'C Cuts at: 1790 2036 2693 4287 5443 1790 Size: 246 657 1594 1156 2573 Fragments arranged by size: 2573 1594 1156 657 246 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6226 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2267 2335 2544 Size: 416 53 83 399 24 850 68 209 Cuts at: 2544 3957 4915 442 Size: 1413 958 1753 Fragments arranged by size: 1753 1413 958 850 416 399 209 83 68 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6226 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4886 1239 Size: 3647 2579 Bpu10I CC'TnA_GC Cuts at: 3425 3425 Size: 6226 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4351 5216 5514 4351 Size: 865 298 5063 Fragments arranged by size: 5063 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2315 4889 882 Size: 617 816 2574 2219 Fragments arranged by size: 2574 2219 816 617 BsaAI yAC'GTr Cuts at: 493 1204 4017 493 Size: 711 2813 2702 Fragments arranged by size: 2813 2702 711 BsaBI GATnn'nnATC Cuts at: 3375 3440 6220 3375 Size: 65 2780 3381 Fragments arranged by size: 3381 2780 65 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4033 4415 5050 275 Size: 53 83 186 936 2500 382 635 1451 Fragments arranged by size: 2500 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2039 4659 5057 5331 5478 5889 2039 Size: 2620 398 274 147 411 2376 Fragments arranged by size: 2620 2376 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2317 2347 1178 Size: 30 165 30 914 30 5057 Fragments arranged by size: 5057 914 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6226 BseYI C'CCAG_C Cuts at: 5429 5925 5429 Size: 496 5730 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2859 1163 Size: 573 1123 4530 Fragments arranged by size: 4530 1123 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3676 4437 5465 1540 Size: 275 186 1675 761 1028 2301 Fragments arranged by size: 2301 1675 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2374 4287 4372 5443 2374 Size: 1913 85 1071 3157 Fragments arranged by size: 3157 1913 1071 85 BsmI GAATG_Cn' Cuts at: 2117 3737 3830 2117 Size: 1620 93 4513 Fragments arranged by size: 4513 1620 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2026 2036 2374 2693 3545 4287 4372 Size: 399 236 10 338 319 852 742 85 Cuts at: 4372 5443 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 852 742 399 338 319 236 85 10 BspEI T'CCGG_A Cuts at: 5889 5889 Size: 6226 BspHI T'CATG_A Cuts at: 4115 5845 4115 Size: 1730 4496 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2099 3581 844 Size: 1255 1482 3489 Fragments arranged by size: 3489 1482 1255 BsrBI CCG'CTC Cuts at: 1394 1823 4113 1394 Size: 429 2290 3507 Fragments arranged by size: 3507 2290 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2639 2699 4721 4895 65 Size: 1183 1391 60 2022 174 1396 Fragments arranged by size: 2022 1396 1391 1183 174 60 BsrFI r'CCGG_y Cuts at: 1700 1810 4870 5057 1700 Size: 110 3060 187 2869 Fragments arranged by size: 3060 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6226 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6226 BssSI C'ACGA_G Cuts at: 4286 5298 4286 Size: 1012 5214 BstAPI GCAn_nnn'nTGC Cuts at: 794 2114 794 Size: 1320 4906 BstBI TT'CG_AA Cuts at: 2611 5030 2611 Size: 2419 3807 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6226 BstYI r'GATC_y Cuts at: 1972 3145 3347 3376 3556 3923 4308 4325 5777 Size: 1173 202 29 180 367 385 17 1452 Cuts at: 5777 5863 5875 6221 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1173 385 367 346 202 180 86 29 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2058 2067 513 Size: 552 939 54 9 4672 Fragments arranged by size: 4672 939 552 54 9 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5487 Fragments arranged by size: 5487 683 56 BtsI GCAGTG_nn' Cuts at: 3742 4536 4556 3742 Size: 794 20 5412 Fragments arranged by size: 5412 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6226 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6191 DraI TTT'AAA Cuts at: 3530 3530 Size: 6226 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5233 809 Size: 507 3917 1802 Fragments arranged by size: 3917 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3673 4562 6191 Size: 54 1425 50 275 18 1843 889 1629 Cuts at: 6191 8 Size: 43 Fragments arranged by size: 1843 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3673 1537 Size: 275 1861 4090 Fragments arranged by size: 4090 1861 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2318 2761 3191 3219 4156 1353 Size: 965 443 430 28 937 3423 Fragments arranged by size: 3423 965 937 443 430 28 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2472 4813 5187 5333 387 Size: 1064 138 883 2341 374 146 1280 Fragments arranged by size: 2341 1280 1064 883 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2299 3035 3090 3372 4289 4886 5673 Size: 393 667 736 55 282 917 597 787 Cuts at: 5673 5941 1239 Size: 268 1524 Fragments arranged by size: 1524 917 787 736 667 597 393 282 268 55 EcoO109I rG'GnC_Cy Cuts at: 3169 3169 Size: 6226 EcoRI G'AATT_C Cuts at: 2492 3535 3913 2492 Size: 1043 378 4805 Fragments arranged by size: 4805 1043 378 EcoRV GAT'ATC Cuts at: 3061 3061 Size: 6226 FspI TGC'GCA Cuts at: 4732 4732 Size: 6226 HaeII r_GCGC'y Cuts at: 1678 5373 1678 Size: 3695 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3045 3050 3077 3082 3313 Size: 32 135 32 1473 5 27 5 231 Cuts at: 3313 3345 3501 3533 4867 4899 4941 4973 1373 Size: 32 156 32 1334 32 42 32 2626 Fragments arranged by size: 2626 1473 1334 231 156 135 42 32 32 32 32 32 32 27 5 5 HincII GTy'rAC Cuts at: 669 1657 1938 3570 3816 669 Size: 988 281 1632 246 3079 Fragments arranged by size: 3079 1632 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6226 HpaI GTT'AAC Cuts at: 3816 3816 Size: 6226 KpnI G_GTAC'C Cuts at: 2046 3551 2046 Size: 1505 4721 MfeI C'AATT_G Cuts at: 2822 3825 2822 Size: 1003 5223 MluI A'CGCG_T Cuts at: 6102 6102 Size: 6226 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2370 2408 5340 5524 1305 Size: 1065 38 2932 184 2007 Fragments arranged by size: 2932 2007 1065 184 38 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4404 Fragments arranged by size: 4404 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4184 4543 4702 518 Size: 648 3018 359 159 2042 Fragments arranged by size: 3018 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2048 2060 2069 2353 3244 3642 4319 5467 Size: 442 12 9 284 891 398 677 1148 Cuts at: 5467 5712 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 891 677 442 398 284 245 12 9 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4735 Fragments arranged by size: 4735 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6226 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6226 NotI GC'GGCC_GC Cuts at: 3673 3673 Size: 6226 NspI r_CATG'y Cuts at: 1132 1229 2548 3584 5129 1132 Size: 97 1319 1036 1545 2229 Fragments arranged by size: 2229 1545 1319 1036 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6226 PciI A'CATG_T Cuts at: 5125 5125 Size: 6226 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6226 PmeI GTTT'AAAC Cuts at: 3530 3530 Size: 6226 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4295 4327 5808 5840 4295 Size: 32 1481 32 4681 Fragments arranged by size: 4681 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3169 3169 Size: 6226 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6226 PsiI TTA'TAA Cuts at: 3796 3796 Size: 6226 PstI C_TGCA'G Cuts at: 830 2053 2110 2439 3578 830 Size: 1223 57 329 1139 3478 Fragments arranged by size: 3478 1223 1139 329 57 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6226 PvuII CAG'CTG Cuts at: 2353 3244 2353 Size: 891 5335 SacII CC_GC'GG Cuts at: 2061 2070 2061 Size: 9 6217 SalI G'TCGA_C Cuts at: 1936 3568 1936 Size: 1632 4594 SapI GCTCTTCn'nnn_ Cuts at: 3219 3219 Size: 6226 SbfI CC_TGCA'GG Cuts at: 3578 3578 Size: 6226 ScaI AGT'ACT Cuts at: 2227 4474 6186 2227 Size: 2247 1712 2267 Fragments arranged by size: 2267 2247 1712 SfcI C'TryA_G Cuts at: 826 1045 2049 2106 2435 3108 3574 4709 5390 Size: 219 1004 57 329 673 466 1135 681 Cuts at: 5390 5581 826 Size: 191 1471 Fragments arranged by size: 1471 1135 1004 681 673 466 329 219 191 57 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6226 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6226 SmaI CCC'GGG Cuts at: 1872 3553 1872 Size: 1681 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4330 5231 5493 820 Size: 197 926 2387 901 262 1553 Fragments arranged by size: 2387 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4017 493 Size: 3524 2702 SpeI A'CTAG_T Cuts at: 152 2222 152 Size: 2070 4156 SphI G_CATG'C Cuts at: 1132 2548 3584 1132 Size: 1416 1036 3774 Fragments arranged by size: 3774 1416 1036 SspI AAT'ATT Cuts at: 5 52 2479 4150 5 Size: 47 2427 1671 2081 Fragments arranged by size: 2427 2081 1671 47 StuI AGG'CCT Cuts at: 1517 2441 3423 1517 Size: 924 982 4320 Fragments arranged by size: 4320 982 924 StyI C'CwwG_G Cuts at: 513 1065 2004 2940 3665 513 Size: 552 939 936 725 3074 Fragments arranged by size: 3074 939 936 725 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4253 4270 4423 4608 5053 325 Size: 1255 2673 17 153 185 445 1498 Fragments arranged by size: 2673 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2225 4472 6184 Size: 274 80 33 51 1443 248 2247 1712 Cuts at: 6184 96 Size: 138 Fragments arranged by size: 2247 1712 1443 274 248 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6186 6218 6186 Size: 32 6194 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6226 XbaI T'CTAG_A Cuts at: 3457 3562 3457 Size: 105 6121 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3509 3509 Size: 6226 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6226 XmaI C'CCGG_G Cuts at: 1870 3551 1870 Size: 1681 4545 XmnI GAAnn'nnTTC Cuts at: 4355 4355 Size: 6226 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4034 276 Size: 53 83 186 936 2500 2468 Fragments arranged by size: 2500 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SanDI SexAI SfiI SfoI SrfI SwaI