(Circular) (Six-base) MAPSORT of: pFN21AB5787.seq Check: 7461 from: 1 to: 7192 pFN21AB5787 7192 bp With 182 enzymes: SgfI * April 15, 2010 09:29 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2701 2701 Size: 7192 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5002 278 Size: 53 83 186 936 3466 2468 Fragments arranged by size: 3466 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3387 4535 1937 Size: 1450 1148 4594 Fragments arranged by size: 4594 1450 1148 Acc65I G'GTAC_C Cuts at: 4513 4513 Size: 7192 AclI AA'CG_TT Cuts at: 1563 5319 5692 1563 Size: 3756 373 3063 Fragments arranged by size: 3756 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2080 2127 2285 3278 3842 3854 4310 5255 Size: 448 47 158 993 564 12 456 945 Cuts at: 5255 6639 6907 1632 Size: 1384 268 1917 Fragments arranged by size: 1917 1384 993 945 564 456 448 268 158 47 12 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6995 AflIII A'CryG_T Cuts at: 1203 4467 6091 7068 1203 Size: 3264 1624 977 1327 Fragments arranged by size: 3264 1624 1327 977 AgeI A'CCGG_T Cuts at: 6023 6023 Size: 7192 AhdI GACnn_n'nnGTC Cuts at: 4372 5921 4372 Size: 1549 5643 AleI CACnn'nnGTG Cuts at: 1166 2939 3085 3284 3494 1166 Size: 1773 146 199 210 4864 Fragments arranged by size: 4864 1773 210 199 146 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7192 AlwNI CAG_nnn'CTG Cuts at: 2155 2386 3049 3647 3697 4935 6507 2155 Size: 231 663 598 50 1238 1572 2840 Fragments arranged by size: 2840 1572 1238 663 598 231 50 ApaLI G'TGCA_C Cuts at: 3854 5249 6405 3854 Size: 1395 1156 4641 Fragments arranged by size: 4641 1395 1156 ApoI r'AATT_y Cuts at: 1493 4501 4727 4879 6988 1493 Size: 3008 226 152 2109 1697 Fragments arranged by size: 3008 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5746 160 Size: 872 4714 1606 Fragments arranged by size: 4714 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7192 AvaI C'yCGr_G Cuts at: 1870 1943 4517 1870 Size: 73 2574 4545 Fragments arranged by size: 4545 2574 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2688 2721 4217 4250 2688 Size: 33 1496 33 5630 Fragments arranged by size: 5630 1496 33 33 BamHI G'GATC_C Cuts at: 4522 4889 4522 Size: 367 6825 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2579 2834 3313 3500 4207 Size: 325 217 627 792 255 479 187 707 Cuts at: 4207 4513 5968 618 Size: 306 1455 1842 Fragments arranged by size: 1842 1455 792 707 627 479 325 306 255 217 187 BanII G_rGCy'C Cuts at: 1391 2013 2145 2553 3654 4062 4385 4511 1391 Size: 622 132 408 1101 408 323 126 4072 Fragments arranged by size: 4072 1101 622 408 408 323 132 126 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2086 2320 2467 3405 4090 928 Size: 971 187 234 147 938 685 4030 Fragments arranged by size: 4030 971 938 685 234 187 147 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5383 5417 1903 Size: 34 3446 34 3678 Fragments arranged by size: 3678 3446 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3835 5084 6294 3835 Size: 1249 1210 4733 Fragments arranged by size: 4733 1249 1210 BclI T'GATC_A Cuts at: 1548 3900 1548 Size: 2352 4840 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5803 136 Size: 107 122 71 631 605 4131 1525 Fragments arranged by size: 4131 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2286 7187 2286 Size: 4901 2291 BlpI GC'TnA_GC Cuts at: 4609 4609 Size: 7192 Bme1580I G_kGCm'C Cuts at: 1790 3503 3858 5253 6409 1790 Size: 1713 355 1395 1156 2573 Fragments arranged by size: 2573 1713 1395 1156 355 BmgBI CAC'GTC Cuts at: 1318 3853 4204 1318 Size: 2535 351 4306 Fragments arranged by size: 4306 2535 351 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3378 3635 4923 Size: 416 53 83 399 24 1961 257 1288 Cuts at: 4923 5881 442 Size: 958 1753 Fragments arranged by size: 1961 1753 1288 958 416 399 257 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3464 1056 Size: 2408 4784 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2030 2836 4190 5852 1239 Size: 791 806 1354 1662 2579 Fragments arranged by size: 2579 1662 1354 806 791 Bpu10I CC'TnA_GC Cuts at: 3648 4379 3648 Size: 731 6461 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2381 5317 6182 6480 2381 Size: 2936 865 298 3093 Fragments arranged by size: 3093 2936 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3438 5855 882 Size: 617 1939 2417 2219 Fragments arranged by size: 2417 2219 1939 617 BsaAI yAC'GTr Cuts at: 493 1204 3295 4983 493 Size: 711 2091 1688 2702 Fragments arranged by size: 2702 2091 1688 711 BsaBI GATnn'nnATC Cuts at: 3665 7186 3665 Size: 3521 3671 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3069 4368 4999 5381 Size: 53 83 186 936 1536 1299 631 382 Cuts at: 5381 6016 275 Size: 635 1451 Fragments arranged by size: 1536 1451 1299 936 635 631 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2823 5625 6023 6297 6444 6855 2823 Size: 2802 398 274 147 411 3160 Fragments arranged by size: 3160 2802 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3365 3395 1178 Size: 30 165 30 1962 30 4975 Fragments arranged by size: 4975 1962 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2027 2546 4384 1186 Size: 841 519 1838 3994 Fragments arranged by size: 3994 1838 841 519 BseYI C'CCAG_C Cuts at: 2155 3448 3747 3982 4152 6395 6891 2155 Size: 1293 299 235 170 2243 496 2456 Fragments arranged by size: 2456 2243 1293 496 299 235 170 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3062 1163 Size: 573 1326 5293 Fragments arranged by size: 5293 1326 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3511 4642 5403 6431 1540 Size: 275 186 1510 1131 761 1028 2301 Fragments arranged by size: 2301 1510 1131 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2553 2831 3858 4385 5253 5338 6409 2553 Size: 278 1027 527 868 85 1071 3336 Fragments arranged by size: 3336 1071 1027 868 527 278 85 BsmI GAATG_Cn' Cuts at: 3028 4703 4796 3028 Size: 1675 93 5424 Fragments arranged by size: 5424 1675 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2037 2037 Size: 7192 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2013 2145 2553 2831 3503 3654 3858 Size: 399 223 132 408 278 672 151 204 Cuts at: 3858 4062 4385 4511 5253 5338 6409 1391 Size: 204 323 126 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 672 408 399 323 278 223 204 204 151 132 126 85 BspEI T'CCGG_A Cuts at: 6855 6855 Size: 7192 BspHI T'CATG_A Cuts at: 5081 6811 5081 Size: 1730 5462 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2701 3475 3950 4547 844 Size: 1857 774 475 597 3489 Fragments arranged by size: 3489 1857 774 597 475 BsrBI CCG'CTC Cuts at: 1394 1823 5079 1394 Size: 429 3256 3507 Fragments arranged by size: 3507 3256 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4204 5687 5861 65 Size: 1183 2956 1483 174 1396 Fragments arranged by size: 2956 1483 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5836 6023 1700 Size: 110 4026 187 2869 Fragments arranged by size: 4026 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7192 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7192 BssSI C'ACGA_G Cuts at: 2016 5252 6264 2016 Size: 3236 1012 2944 Fragments arranged by size: 3236 2944 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7192 BstBI TT'CG_AA Cuts at: 5996 5996 Size: 7192 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7192 BstXI CCAn_nnnn'nTGG Cuts at: 2860 2911 2941 3286 3456 2860 Size: 51 30 345 170 6596 Fragments arranged by size: 6596 345 170 51 30 BstYI r'GATC_y Cuts at: 1972 2286 2955 4522 4889 5274 5291 6743 6829 Size: 314 669 1567 367 385 17 1452 86 Cuts at: 6829 6841 7187 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1567 1452 669 385 367 346 314 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2466 2466 Size: 7192 BtgI C'CryG_G Cuts at: 513 1065 2004 2721 2934 3465 3564 3634 3874 Size: 552 939 717 213 531 99 70 240 Cuts at: 3874 3925 513 Size: 51 3780 Fragments arranged by size: 3780 939 717 552 531 240 213 99 70 51 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6453 Fragments arranged by size: 6453 683 56 BtsI GCAGTG_nn' Cuts at: 2069 2946 3033 3390 4708 5502 5522 2069 Size: 877 87 357 1318 794 20 3739 Fragments arranged by size: 3739 1318 877 794 357 87 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7192 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7157 DraI TTT'AAA Cuts at: 4496 4496 Size: 7192 DraIII CAC_nnn'GTG Cuts at: 2566 3915 2566 Size: 1349 5843 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3809 6199 809 Size: 507 2493 2390 1802 Fragments arranged by size: 2493 2390 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2146 2557 3561 Size: 54 1425 50 275 18 316 411 1004 Cuts at: 3561 3928 4244 4639 5528 7157 8 Size: 367 316 395 889 1629 43 Fragments arranged by size: 1629 1425 1004 889 411 395 367 316 316 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4639 1537 Size: 275 2827 4090 Fragments arranged by size: 4090 2827 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4107 4122 5122 1353 Size: 2754 15 1000 3423 Fragments arranged by size: 3423 2754 1000 15 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4387 5779 6153 6299 387 Size: 1064 138 2798 1392 374 146 1280 Fragments arranged by size: 2798 1392 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2551 4383 2551 Size: 1832 5360 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2030 2080 2127 2285 2836 3278 3842 Size: 393 398 50 47 158 551 442 564 Cuts at: 3842 3854 4190 4310 5255 5852 6639 6907 1239 Size: 12 336 120 945 597 787 268 1524 Fragments arranged by size: 1524 945 787 597 564 551 442 398 393 336 268 158 120 50 47 12 EcoNI CCTnn'n_nnAGG Cuts at: 2045 3836 2045 Size: 1791 5401 EcoO109I rG'GnC_Cy Cuts at: 2369 2593 2794 3244 3715 2369 Size: 224 201 450 471 5846 Fragments arranged by size: 5846 471 450 224 201 EcoRI G'AATT_C Cuts at: 4501 4879 4501 Size: 378 6814 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2389 2389 Size: 7192 FspI TGC'GCA Cuts at: 5698 5698 Size: 7192 HaeII r_GCGC'y Cuts at: 1678 2156 6339 1678 Size: 478 4183 2531 Fragments arranged by size: 4183 2531 478 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2374 2406 2658 2690 3954 Size: 32 135 32 802 32 252 32 1264 Cuts at: 3954 3986 4358 4390 5833 5865 5907 5939 1373 Size: 32 372 32 1443 32 42 32 2626 Fragments arranged by size: 2626 1443 1264 802 372 252 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3790 4536 4782 669 Size: 988 281 1852 746 246 3079 Fragments arranged by size: 3079 1852 988 746 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7192 HpaI GTT'AAC Cuts at: 4782 4782 Size: 7192 KpnI G_GTAC'C Cuts at: 4517 4517 Size: 7192 MfeI C'AATT_G Cuts at: 4791 4791 Size: 7192 MluI A'CGCG_T Cuts at: 7068 7068 Size: 7192 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4389 4428 6306 6490 1305 Size: 3084 39 1878 184 2007 Fragments arranged by size: 3084 2007 1878 184 39 MscI TGG'CCA Cuts at: 10 64 1489 1832 2559 3563 3930 10 Size: 54 1425 343 727 1004 367 3272 Fragments arranged by size: 3272 1425 1004 727 367 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2615 2909 2939 3085 3284 3494 3930 Size: 648 1449 294 30 146 199 210 436 Cuts at: 3930 5150 5509 5668 518 Size: 1220 359 159 2042 Fragments arranged by size: 2042 1449 1220 648 436 359 294 210 199 159 146 30 MspA1I CmG'CkG Cuts at: 1606 3494 3892 4359 4608 5285 6433 6678 1606 Size: 1888 398 467 249 677 1148 245 2120 Fragments arranged by size: 2120 1888 1148 677 467 398 249 245 NcoI C'CATG_G Cuts at: 513 1065 2004 3465 3564 3634 3874 3925 513 Size: 552 939 1461 99 70 240 51 3780 Fragments arranged by size: 3780 1461 939 552 240 99 70 51 NdeI CA'TA_TG Cuts at: 387 3847 387 Size: 3460 3732 NheI G'CTAG_C Cuts at: 1052 3460 1052 Size: 2408 4784 NotI GC'GGCC_GC Cuts at: 4639 4639 Size: 7192 NspI r_CATG'y Cuts at: 1132 1229 4471 4550 6095 1132 Size: 97 3242 79 1545 2229 Fragments arranged by size: 3242 2229 1545 97 79 PasI CC'CwG_GG Cuts at: 1348 2180 1348 Size: 832 6360 PciI A'CATG_T Cuts at: 4467 6091 4467 Size: 1624 5568 PflMI CCAn_nnn'nTGG Cuts at: 1108 2155 3479 1108 Size: 1047 1324 4821 Fragments arranged by size: 4821 1324 1047 PfoI T'CCnGG_A Cuts at: 2247 3351 2247 Size: 1104 6088 PmeI GTTT'AAAC Cuts at: 4496 4496 Size: 7192 PmlI CAC'GTG Cuts at: 3295 3295 Size: 7192 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5261 5293 6774 6806 5261 Size: 32 1481 32 5647 Fragments arranged by size: 5647 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2369 2593 2794 3715 2369 Size: 224 201 921 5846 Fragments arranged by size: 5846 921 224 201 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7192 PsiI TTA'TAA Cuts at: 4277 4762 4277 Size: 485 6707 PstI C_TGCA'G Cuts at: 830 2064 3397 4544 830 Size: 1234 1333 1147 3478 Fragments arranged by size: 3478 1333 1234 1147 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7192 PvuII CAG'CTG Cuts at: 3494 3892 3494 Size: 398 6794 SacI G_AGCT'C Cuts at: 2553 4385 2553 Size: 1832 5360 SalI G'TCGA_C Cuts at: 1936 4534 1936 Size: 2598 4594 SanDI GG'GwC_CC Cuts at: 2369 2369 Size: 7192 SbfI CC_TGCA'GG Cuts at: 4544 4544 Size: 7192 ScaI AGT'ACT Cuts at: 5440 7152 5440 Size: 1712 5480 SexAI A'CCwGG_T Cuts at: 2692 2692 Size: 7192 SfcI C'TryA_G Cuts at: 826 1045 2060 2431 2481 3393 4540 5675 6356 Size: 219 1015 371 50 912 1147 1135 681 Cuts at: 6356 6547 826 Size: 191 1471 Fragments arranged by size: 1471 1147 1135 1015 912 681 371 219 191 50 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7192 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7192 SmaI CCC'GGG Cuts at: 1872 4519 1872 Size: 2647 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2396 5296 6197 6459 820 Size: 197 926 453 2900 901 262 1553 Fragments arranged by size: 2900 1553 926 901 453 262 197 SnaBI TAC'GTA Cuts at: 493 4983 493 Size: 4490 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7192 SphI G_CATG'C Cuts at: 1132 4550 1132 Size: 3418 3774 SspI AAT'ATT Cuts at: 5 52 5116 5 Size: 47 5064 2081 Fragments arranged by size: 5064 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7192 StyI C'CwwG_G Cuts at: 513 1065 2004 2115 3465 3564 3634 3874 3925 Size: 552 939 111 1350 99 70 240 51 Cuts at: 3925 4631 513 Size: 706 3074 Fragments arranged by size: 3074 1350 939 706 552 240 111 99 70 51 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2320 2636 3161 4166 4243 5219 5236 Size: 1255 740 316 525 1005 77 976 17 Cuts at: 5236 5389 5574 6019 325 Size: 153 185 445 1498 Fragments arranged by size: 1498 1255 1005 976 740 525 445 316 185 153 77 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2883 5438 7150 Size: 274 80 33 51 1443 906 2555 1712 Cuts at: 7150 96 Size: 138 Fragments arranged by size: 2555 1712 1443 906 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2880 2912 3365 3397 7152 7184 2880 Size: 32 453 32 3755 32 2888 Fragments arranged by size: 3755 2888 453 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 4094 1316 Size: 2778 4414 XbaI T'CTAG_A Cuts at: 4528 4528 Size: 7192 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2938 3187 4183 2938 Size: 249 996 5947 Fragments arranged by size: 5947 996 249 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7192 XmaI C'CCGG_G Cuts at: 1870 4517 1870 Size: 2647 4545 XmnI GAAnn'nnTTC Cuts at: 2165 2288 3827 5321 2165 Size: 123 1539 1494 4036 Fragments arranged by size: 4036 1539 1494 123 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5000 276 Size: 53 83 186 936 3466 2468 Fragments arranged by size: 3466 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BbeI BbvCI BfrBI BplI BsiWI BstZ17I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PspOMI PspXI PsrI RsrII SacII SapI SfiI SfoI SrfI SwaI