Restriction map for
pFN21AB5883
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# NO CUT ENZYMES ( 144 enzymes) #
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AatII Acc65I AccI AccII AclI
AcyI AfaI AflII AflIII AgeI
Alw44I AocI Aor51HI ApaLI AscI
AseI AsnI Asp700I Asp718I AspI
AviII BamHI BanI BbeI BbrPI
BbsI BbvCI BcgI BclI BfrI
BglII BlpI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BsiEI BsiWI BsmBI
BsmI Bsp1407I BspDI BspHI BspLU11I
BspMI BsrBI BsrFI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstUI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DraI DraIII DrdI
EagI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRV EcoT22I FbaI
FseI FspI HaeII HgaI Hin1I
HindIII HpaI KasI KpnI KspI
MaeIII MamI MfeI MluI MseI
MspA1I MunI MvnI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsaI RsrII SacI SacII
SalI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I SspBI SspI StuI
SwaI Tru9I Tsp45I Tth111I XbaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 30 enzymes) #
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AccIII 774 TCCGGA T^CCGGA
AhdI 332 GACAGGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 332 GACAGGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 319 CCCGAG C^[CT]CG[GA]G
AvrII 618 CCTAGG C^CTAGG
BciVI 448 GGATAC GTATCC(6/5)
BlnI 618 CCTAGG C^CTAGG
BsaWI 774 TCCGGA [AT]^CCGG[AT]
BseAI 774 TCCGGA T^CCGGA
BsoBI 319 CCCGAG C^[CT]CG[GA]G
BspEI 774 TCCGGA T^CCGGA
BsrDI 979 GCAATG GCAATG(2/0)
BstAPI 163 GCAAGGCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 1283 CCAAGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 753 GCGC GCG^C
DsaI 11 CCACGG C^C[GA][CT]GG
Eam1105I 332 GACAGGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
HhaI 753 GCGC GCG^C
HinP1I 753 GCGC G^CGC
HincII 1093 GTCAAC GT[CT]^[GA]AC
HindII 1093 GTCAAC GT[CT]^[GA]AC
MaeII 194 ACGT A^CGT
MroI 774 TCCGGA T^CCGGA
NspI 168 GCATGC [GA]CATG^[CT]
PleI 1359 GAGTC GAGTC(4/5)
PstI 42 CTGCAG CTGCA^G
SapI 233 GAAGAGC GCTCTTC(1/4)
SmlI 1114 CTTGAG C^T[CT][GA]AG
SphI 168 GCATGC GCATG^C
TfiI 4 GAATC G^A[AT]TC
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# TWO CUT ENZYMES ( 25 enzymes) #
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AcsI 352 GAATTC [GA]^AATT[CT]
1392 GAATTC
AlwNI 823 CAGCTACTG CAG[ACGT][ACGT][ACGT]^CTG
1069 CAGAGACTG
ApoI 352 GAATTC [GA]^AATT[CT]
1392 GAATTC
AspHI 694 GAGCAC G[AT]GC[AT]^C
1256 GAGCAC
BalI 50 TGGCCA TGG^CCA
905 TGGCCA
BcnI 146 CCGGG CC^[GC]GG
297 CCGGG
BglI 219 GCCTGGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
842 GCCTCTGTGGC
BsaI 459 GAGACC GGTCTC(1/5)
1293 GGTCTC
BsgI 673 CTGCAC GTGCAG(16/14)
1088 GTGCAG
BsiHKAI 694 GAGCAC G[AT]GC[AT]^C
1256 GAGCAC
BstYI 546 AGATCC [GA]^GATC[CT]
1101 AGATCC
EaeI 50 TGGCCA [CT]^GGCC[GA]
905 TGGCCA
EcoRI 352 GAATTC G^AATTC
1392 GAATTC
FauI 855 CCCGC CCCGC(4/6)
1329 CCCGC
HinfI 4 GAATC G^A[ACGT]TC
1359 GAGTC
MflI 546 AGATCC [GA]^GATC[CT]
1101 AGATCC
MluNI 50 TGGCCA TGG^CCA
905 TGGCCA
MscI 50 TGGCCA TGG^CCA
905 TGGCCA
MslI 785 CATTCCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
805 CACCATCATG
NciI 146 CCGGG CC^[GC]GG
297 CCGGG
TaqI 356 TCGA T^CGA
1297 TCGA
Tsp509I 353 AATT ^AATT
1393 AATT
TthHB8I 356 TCGA T^CGA
1297 TCGA
XcmI 314 CCACTCCCGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
498 CCAACAGCAGTATGG
XhoII 546 AGATCC [GA]^GATC[CT]
1101 AGATCC
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# 3 OR MORE CUT ENZYMES ( 59 enzymes) #
#########################################
AciI AluI AlwI ApaI AvaII
BanII BbvI BfaI BmyI BpmI
BsaJI BseRI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI Cac8I Cfr13I DdeI DpnII
DraII EarI EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HpaII
HphI ItaI Ksp632I MaeI MboI
MboII MnlI MspI MvaI MwoI
NdeII NlaIII NlaIV PflMI PpuMI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TseI TspRI Van91I