Restriction map for pFN21AB5883
 (Linear) (Six-base) MAPSORT of: KIBB5883.seq  Check: 2861  from: 1  to: 1401



KIBB5883.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:18  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      82    1401 
   Size:         82    1319
 
AhdI GACnn_n'nnGTC

Cuts at:      0     337    1401 
   Size:        337    1064
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1378    1401 
   Size:       1378      23
 
AlwNI CAG_nnn'CTG

Cuts at:      0     828    1074    1401 
   Size:        828     246     327

  Fragments arranged by size: 

                 828     327     246
 
ApaI G_GGCC'C

Cuts at:      0      20     303     720    1063    1401 
   Size:         20     283     417     343     338

  Fragments arranged by size: 

                 417     343     338     283      20
 
ApoI r'AATT_y

Cuts at:      0     352    1392    1401 
   Size:        352    1040       9

  Fragments arranged by size: 

                1040     352       9
 
AvaI C'yCGr_G

Cuts at:      0     319    1401 
   Size:        319    1082
 
AvrII C'CTAG_G

Cuts at:      0     618    1401 
   Size:        618     783
 
BanII G_rGCy'C

Cuts at:      0      20     303     720     932    1063    1401 
   Size:         20     283     417     212     131     338

  Fragments arranged by size: 

                 417     338     283     212     131      20
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     442    1401 
   Size:        442     959
 
BglI GCCn_nnn'nGGC

Cuts at:      0     225     848    1401 
   Size:        225     623     553

  Fragments arranged by size: 

                 623     553     225
 
Bme1580I G_kGCm'C

Cuts at:      0      20     152     303     617     720     796    1063    1401 
   Size:         20     132     151     314     103      76     267     338

  Fragments arranged by size: 

                 338     314     267     151     132     103      76      20
            
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     723     758    1401 
   Size:        723      35     643

  Fragments arranged by size: 

                 723     643      35
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1311    1401 
   Size:       1311      90
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     260     346    1072    1401 
   Size:        260      86     726     329

  Fragments arranged by size: 

                 726     329     260      86
 
Bpu10I CC'TnA_GC

Cuts at:      0    1181    1401 
   Size:       1181     220
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1135    1401 
   Size:       1135     266
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     453    1299    1401 
   Size:        453     846     102

  Fragments arranged by size: 

                 846     453     102
 
BsaWI w'CCGG_w

Cuts at:      0     774    1401 
   Size:        774     627
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     353     982     999    1008    1401 
   Size:        353     629      17       9     393

  Fragments arranged by size: 

                 629     393     353      17       9
 
BseYI C'CCAG_C

Cuts at:      0      74     204     228     392    1401 
   Size:         74     130      24     164    1009

  Fragments arranged by size: 

                1009     164     130      74      24
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     658    1109    1401 
   Size:        658     451     292

  Fragments arranged by size: 

                 658     451     292
 
BsiHKAI G_wGCw'C

Cuts at:      0     698    1260    1401 
   Size:        698     562     141

  Fragments arranged by size: 

                 698     562     141
 
Bsp1286I G_dGCh'C

Cuts at:      0      20     152     303     617     698     720     796     932 
   Size:         20     132     151     314      81      22      76     136

Cuts at:    932    1063    1260    1401 
   Size:        131     197     141

  Fragments arranged by size: 

                 314     197     151     141     136     132     131      81
                  76      22      20
 
BspEI T'CCGG_A

Cuts at:      0     774    1401 
   Size:        774     627
 
BsrDI GCAATG_nn'

Cuts at:      0     986    1401 
   Size:        986     415
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     169    1401 
   Size:        169    1232
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1290    1401 
   Size:       1290     111
 
BstYI r'GATC_y

Cuts at:      0     546    1101    1401 
   Size:        546     555     300

  Fragments arranged by size: 

                 555     546     300
 
BtgI C'CryG_G

Cuts at:      0      11    1401 
   Size:         11    1390
 
EaeI y'GGCC_r

Cuts at:      0      50     905    1401 
   Size:         50     855     496

  Fragments arranged by size: 

                 855     496      50
 
EarI CTCTTCn'nnn_

Cuts at:      0     180     228     410    1162    1401 
   Size:        180      48     182     752     239

  Fragments arranged by size: 

                 752     239     182     180      48
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      82     260     346    1072    1401 
   Size:         82     178      86     726     329

  Fragments arranged by size: 

                 726     329     178      86      82
 
EcoO109I rG'GnC_Cy

Cuts at:      0      16      66      99     217     261     339     704     717 
   Size:         16      50      33     118      44      78     365      13

Cuts at:    717     890     958    1060    1401 
   Size:        173      68     102     341

  Fragments arranged by size: 

                 365     341     173     118     102      78      68      50
                  44      33      16      13
 
EcoRI G'AATT_C

Cuts at:      0     352    1392    1401 
   Size:        352    1040       9

  Fragments arranged by size: 

                1040     352       9
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     323     355     531     563    1047    1079    1401 
   Size:        323      32     176      32     484      32     322

  Fragments arranged by size: 

                 484     323     322     176      32      32      32
 
HincII GTy'rAC

Cuts at:      0    1095    1401 
   Size:       1095     306
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     905    1241    1401 
   Size:        905     336     160

  Fragments arranged by size: 

                 905     336     160
 
MscI TGG'CCA

Cuts at:      0      52     907    1401 
   Size:         52     855     494

  Fragments arranged by size: 

                 855     494      52
 
MslI CAynn'nnrTG

Cuts at:      0     789     809    1401 
   Size:        789      20     592

  Fragments arranged by size: 

                 789     592      20
 
NspI r_CATG'y

Cuts at:      0     172    1401 
   Size:        172    1229
 
PasI CC'CwG_GG

Cuts at:      0    1192    1401 
   Size:       1192     209
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     257     748    1192    1401 
   Size:        257     491     444     209

  Fragments arranged by size: 

                 491     444     257     209
 
PpuMI rG'GwC_Cy

Cuts at:      0      99     261     339     890     958    1401 
   Size:         99     162      78     551      68     443

  Fragments arranged by size: 

                 551     443     162      99      78      68
 
PspOMI G'GGCC_C

Cuts at:      0      16     299     716    1059    1401 
   Size:         16     283     417     343     342

  Fragments arranged by size: 

                 417     343     342     283      16
 
PstI C_TGCA'G

Cuts at:      0      46    1401 
   Size:         46    1355
 
SapI GCTCTTCn'nnn_

Cuts at:      0     228    1401 
   Size:        228    1173
 
SfcI C'TryA_G

Cuts at:      0      42     443    1265    1401 
   Size:         42     401     822     136

  Fragments arranged by size: 

                 822     401     136      42
 
SmlI C'TyrA_G

Cuts at:      0    1114    1401 
   Size:       1114     287
 
SphI G_CATG'C

Cuts at:      0     172    1401 
   Size:        172    1229
 
StyI C'CwwG_G

Cuts at:      0      94     618    1283    1401 
   Size:         94     524     665     118

  Fragments arranged by size: 

                 665     524     118      94
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     321     505    1401 
   Size:        321     184     896

  Fragments arranged by size: 

                 896     321     184
 

 Enzymes that do cut:

     AcuI     AhdI     AloI    AlwNI     ApaI     ApoI     AvaI    AvrII
    BanII    BciVI     BglI Bme1580I     BmrI     BplI     BpmI   Bpu10I
    BpuEI     BsaI    BsaWI    BseRI    BseYI     BsgI  BsiHKAI Bsp1286I
    BspEI    BsrDI   BstAPI    BstXI    BstYI     BtgI     EaeI     EarI
  Eco57MI EcoO109I    EcoRI    Hin4I   HincII     MmeI     MscI     MslI
     NspI     PasI    PflMI    PpuMI   PspOMI     PstI     SapI     SfcI
     SmlI     SphI     StyI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AleI     AlfI    ApaLI     AscI     AseI    AsiSI     BaeI
    BamHI     BanI     BbeI     BbsI    BbvCI     BcgI     BclI    BfrBI
    BglII     BlpI    BmgBI     BmtI    BsaAI    BsaBI    BsaHI    BsaXI
    BsiEI    BsiWI     BsmI    BsmBI    BspHI    BspMI    BsrBI    BsrFI
    BsrGI   BssHII    BssSI    BstBI   BstEII  BstZ17I   Bsu36I    BtgZI
     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EagI     EciI
  EcoICRI    EcoNI    EcoRV     FalI     FseI     FspI    FspAI    HaeII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI   MspA1I     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     PacI     PciI     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI
    PspXI     PsrI     PvuI    PvuII    RsrII     SacI    SacII     SalI
    SanDI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SrfI     SspI     StuI     SwaI    TaqII
     TatI     TstI  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI
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