(Circular) (Six-base) MAPSORT of: pFN21AB5736.seq Check: 9291 from: 1 to: 6913 pFN21AB5736 6913 bp With 182 enzymes: SgfI * April 15, 2010 09:29 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2950 2950 Size: 6913 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2756 4723 278 Size: 53 83 186 936 1220 1967 2468 Fragments arranged by size: 2468 1967 1220 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3785 4256 1937 Size: 1848 471 4594 Fragments arranged by size: 4594 1848 471 Acc65I G'GTAC_C Cuts at: 4234 4234 Size: 6913 AclI AA'CG_TT Cuts at: 1563 5040 5413 1563 Size: 3477 373 3063 Fragments arranged by size: 3477 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2746 3579 4976 6360 6628 1632 Size: 1114 833 1397 1384 268 1917 Fragments arranged by size: 1917 1397 1384 1114 833 268 AflII C'TTAA_G Cuts at: 820 1017 2702 820 Size: 197 1685 5031 Fragments arranged by size: 5031 1685 197 AflIII A'CryG_T Cuts at: 1203 5812 6789 1203 Size: 4609 977 1327 Fragments arranged by size: 4609 1327 977 AgeI A'CCGG_T Cuts at: 5744 5744 Size: 6913 AhdI GACnn_n'nnGTC Cuts at: 3317 3476 5642 3317 Size: 159 2166 4588 Fragments arranged by size: 4588 2166 159 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6913 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2703 1184 Size: 1519 5394 AlwNI CAG_nnn'CTG Cuts at: 3308 4656 6228 3308 Size: 1348 1572 3993 Fragments arranged by size: 3993 1572 1348 ApaI G_GGCC'C Cuts at: 3838 3938 4108 3838 Size: 100 170 6643 Fragments arranged by size: 6643 170 100 ApaLI G'TGCA_C Cuts at: 3026 4970 6126 3026 Size: 1944 1156 3813 Fragments arranged by size: 3813 1944 1156 ApoI r'AATT_y Cuts at: 1493 2214 4222 4448 4600 6709 1493 Size: 721 2008 226 152 2109 1697 Fragments arranged by size: 2109 2008 1697 721 226 152 AseI AT'TA_AT Cuts at: 160 1032 5467 160 Size: 872 4435 1606 Fragments arranged by size: 4435 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6913 AvaI C'yCGr_G Cuts at: 1870 1943 2294 2322 2369 3824 3839 4238 1870 Size: 73 351 28 47 1455 15 399 4545 Fragments arranged by size: 4545 1455 399 351 73 47 28 15 BamHI G'GATC_C Cuts at: 3070 4243 4610 3070 Size: 1173 367 5373 Fragments arranged by size: 5373 1173 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2159 2335 3167 3965 4234 Size: 325 217 627 372 176 832 798 269 Cuts at: 4234 5689 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 832 798 627 372 325 269 217 176 BanII G_rGCy'C Cuts at: 1391 2588 3114 3371 3838 3876 3938 3985 3996 Size: 1197 526 257 467 38 62 47 11 Cuts at: 3996 4108 4130 4232 1391 Size: 112 22 102 4072 Fragments arranged by size: 4072 1197 526 467 257 112 102 62 47 38 22 11 BbeI G_GCGC'C Cuts at: 2163 2339 2163 Size: 176 6737 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3508 928 Size: 971 1609 4333 Fragments arranged by size: 4333 1609 971 BbvCI CC'TCA_GC Cuts at: 3014 3014 Size: 6913 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2104 2138 5104 5138 1903 Size: 34 167 34 2966 34 3678 Fragments arranged by size: 3678 2966 167 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2272 2570 3598 4805 6015 2272 Size: 298 1028 1207 1210 3170 Fragments arranged by size: 3170 1210 1207 1028 298 BclI T'GATC_A Cuts at: 1548 1548 Size: 6913 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3888 5524 136 Size: 107 122 71 631 605 2216 1636 1525 Fragments arranged by size: 2216 1636 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6908 6908 Size: 6913 BlpI GC'TnA_GC Cuts at: 4330 4330 Size: 6913 Bme1580I G_kGCm'C Cuts at: 1790 3030 3838 3938 3970 4108 4137 4974 6130 Size: 1240 808 100 32 138 29 837 1156 Cuts at: 6130 1790 Size: 2573 Fragments arranged by size: 2573 1240 1156 837 808 138 100 32 29 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6913 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2031 3969 4644 Size: 416 53 83 399 24 614 1938 675 Cuts at: 4644 5602 442 Size: 958 1753 Fragments arranged by size: 1938 1753 958 675 614 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6913 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2605 2605 Size: 6913 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2292 2325 2465 2579 2633 2765 3285 3568 Size: 1053 33 140 114 54 132 520 283 Cuts at: 3568 3862 4059 4068 4095 5573 1239 Size: 294 197 9 27 1478 2579 Fragments arranged by size: 2579 1478 1053 520 294 283 197 140 132 114 54 33 27 9 Bpu10I CC'TnA_GC Cuts at: 3014 3414 3014 Size: 400 6513 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2380 2536 3323 5038 5903 6201 2380 Size: 156 787 1715 865 298 3092 Fragments arranged by size: 3092 1715 865 787 298 156 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3257 3516 4113 5576 882 Size: 617 1758 259 597 1463 2219 Fragments arranged by size: 2219 1758 1463 617 597 259 BsaAI yAC'GTr Cuts at: 493 1204 3400 4704 493 Size: 711 2196 1304 2702 Fragments arranged by size: 2702 2196 1304 711 BsaBI GATnn'nnATC Cuts at: 3347 6907 3347 Size: 3560 3353 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2160 2336 2753 4720 Size: 53 83 186 936 627 176 417 1967 Cuts at: 4720 5102 5737 275 Size: 382 635 1451 Fragments arranged by size: 1967 1451 936 635 627 417 382 186 176 83 53 BsaWI w'CCGG_w Cuts at: 5346 5744 6018 6165 6576 5346 Size: 398 274 147 411 5683 Fragments arranged by size: 5683 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2570 2600 2738 2768 2879 Size: 30 165 30 1167 30 138 30 111 Cuts at: 2879 2909 3566 3596 3598 3628 1178 Size: 30 657 30 2 30 4463 Fragments arranged by size: 4463 1167 657 165 138 111 30 30 30 30 30 30 30 2 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2578 2657 3069 3184 3606 3842 3930 1186 Size: 1392 79 412 115 422 236 88 4169 Fragments arranged by size: 4169 1392 422 412 236 115 88 79 BseYI C'CCAG_C Cuts at: 2525 3066 3175 4187 6116 6612 2525 Size: 541 109 1012 1929 496 2826 Fragments arranged by size: 2826 1929 1012 541 496 109 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 4112 1163 Size: 573 2376 3964 Fragments arranged by size: 3964 2376 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2925 4363 5124 6152 1540 Size: 275 186 924 1438 761 1028 2301 Fragments arranged by size: 2301 1438 1028 924 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2588 3030 3206 4974 5059 6130 2588 Size: 442 176 1768 85 1071 3371 Fragments arranged by size: 3371 1768 1071 442 176 85 BsmI GAATG_Cn' Cuts at: 4424 4517 4424 Size: 93 6820 BsmBI CGTCTCn'nnnn_ Cuts at: 2036 2868 2036 Size: 832 6081 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2588 3030 3114 3206 3371 3838 3876 Size: 399 798 442 84 92 165 467 38 Cuts at: 3876 3938 3970 3985 3996 4108 4130 4137 4232 Size: 62 32 15 11 112 22 7 95 Cuts at: 4232 4974 5059 6130 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 798 742 467 442 399 165 112 95 92 85 84 62 38 32 22 15 11 7 BspEI T'CCGG_A Cuts at: 6576 6576 Size: 6913 BspHI T'CATG_A Cuts at: 2598 2901 4053 4802 6532 2598 Size: 303 1152 749 1730 2979 Fragments arranged by size: 2979 1730 1152 749 303 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2649 2950 4268 844 Size: 1805 301 1318 3489 Fragments arranged by size: 3489 1805 1318 301 BsrBI CCG'CTC Cuts at: 1394 1823 2491 2869 3418 3832 4800 1394 Size: 429 668 378 549 414 968 3507 Fragments arranged by size: 3507 968 668 549 429 414 378 BsrDI GCAATG_nn' Cuts at: 65 1248 2869 5408 5582 65 Size: 1183 1621 2539 174 1396 Fragments arranged by size: 2539 1621 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2712 5557 5744 1700 Size: 110 902 2845 187 2869 Fragments arranged by size: 2869 2845 902 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6913 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6913 BssSI C'ACGA_G Cuts at: 4973 5985 4973 Size: 1012 5901 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6913 BstBI TT'CG_AA Cuts at: 5717 5717 Size: 6913 BstEII G'GTnAC_C Cuts at: 1184 3280 1184 Size: 2096 4817 BstXI CCAn_nnnn'nTGG Cuts at: 2540 2611 3292 4004 4048 2540 Size: 71 681 712 44 5405 Fragments arranged by size: 5405 712 681 71 44 BstYI r'GATC_y Cuts at: 1972 2094 3070 4243 4610 4995 5012 6464 6550 Size: 122 976 1173 367 385 17 1452 86 Cuts at: 6550 6562 6908 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1173 976 385 367 346 122 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3081 3081 Size: 6913 BtgI C'CryG_G Cuts at: 513 1065 2004 2154 2673 3153 3291 4176 513 Size: 552 939 150 519 480 138 885 3250 Fragments arranged by size: 3250 939 885 552 519 480 150 138 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3942 487 Size: 683 56 2716 3458 Fragments arranged by size: 3458 2716 683 56 BtsI GCAGTG_nn' Cuts at: 2518 2692 3509 3748 3784 3862 3868 4095 4429 Size: 174 817 239 36 78 6 227 334 Cuts at: 4429 5223 5243 2518 Size: 794 20 4188 Fragments arranged by size: 4188 817 794 334 239 227 174 78 36 20 6 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6913 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6878 DraI TTT'AAA Cuts at: 4217 4217 Size: 6913 DraIII CAC_nnn'GTG Cuts at: 3794 3794 Size: 6913 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5920 809 Size: 507 4604 1802 Fragments arranged by size: 4604 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2571 2854 2922 Size: 54 1425 50 275 18 741 283 68 Cuts at: 2922 3057 4173 4360 5249 6878 8 Size: 135 1116 187 889 1629 43 Fragments arranged by size: 1629 1425 1116 889 741 283 275 187 135 68 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2922 4360 1537 Size: 275 1110 1438 4090 Fragments arranged by size: 4090 1438 1110 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2067 4843 1353 Size: 714 2776 3423 Fragments arranged by size: 3423 2776 714 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5500 5874 6020 387 Size: 1064 138 3911 374 146 1280 Fragments arranged by size: 3911 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2586 2586 Size: 6913 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2292 2325 2465 2579 2633 2746 2765 Size: 393 660 33 140 114 54 113 19 Cuts at: 2765 3285 3568 3579 3862 4059 4068 4095 4976 Size: 520 283 11 283 197 9 27 881 Cuts at: 4976 5573 6360 6628 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 881 787 660 597 520 393 283 283 268 197 140 114 113 54 33 27 19 11 9 EcoNI CCTnn'n_nnAGG Cuts at: 2066 2066 Size: 6913 EcoO109I rG'GnC_Cy Cuts at: 3835 3934 3935 4104 4105 3835 Size: 99 1 169 1 6643 Fragments arranged by size: 6643 169 99 1 1 EcoRI G'AATT_C Cuts at: 4222 4600 4222 Size: 378 6535 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2848 3508 2848 Size: 660 6253 FspI TGC'GCA Cuts at: 5419 5419 Size: 6913 HaeII r_GCGC'y Cuts at: 1678 2163 2339 2526 2566 2710 6060 1678 Size: 485 176 187 40 144 3350 2531 Fragments arranged by size: 3350 2531 485 187 176 144 40 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2290 2322 2608 2640 2738 Size: 32 135 32 718 32 286 32 98 Cuts at: 2738 2770 3260 3292 3303 3335 3462 3494 3704 Size: 32 490 32 11 32 127 32 210 Cuts at: 3704 3736 3833 3865 4112 4144 5554 5586 5628 Size: 32 97 32 247 32 1410 32 42 Cuts at: 5628 5660 1373 Size: 32 2626 Fragments arranged by size: 2626 1410 718 490 286 247 210 135 127 98 97 42 32 32 32 32 32 32 32 32 32 32 32 32 32 11 HincII GTy'rAC Cuts at: 669 1657 1938 3382 3481 3556 3786 4257 4503 Size: 988 281 1444 99 75 230 471 246 Cuts at: 4503 669 Size: 3079 Fragments arranged by size: 3079 1444 988 471 281 246 230 99 75 HindIII A'AGCT_T Cuts at: 748 4162 748 Size: 3414 3499 HpaI GTT'AAC Cuts at: 4503 4503 Size: 6913 KasI G'GCGC_C Cuts at: 2159 2335 2159 Size: 176 6737 KpnI G_GTAC'C Cuts at: 4238 4238 Size: 6913 MfeI C'AATT_G Cuts at: 3123 4512 3123 Size: 1389 5524 MluI A'CGCG_T Cuts at: 6789 6789 Size: 6913 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3493 6027 6211 1305 Size: 2188 2534 184 2007 Fragments arranged by size: 2534 2188 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2856 3059 4175 10 Size: 54 1425 343 1024 203 1116 2748 Fragments arranged by size: 2748 1425 1116 1024 343 203 54 MslI CAynn'nnrTG Cuts at: 518 1166 2064 2418 4002 4046 4175 4871 5230 Size: 648 898 354 1584 44 129 696 359 Cuts at: 5230 5389 518 Size: 159 2042 Fragments arranged by size: 2042 1584 898 696 648 359 354 159 129 44 MspA1I CmG'CkG Cuts at: 1606 2149 2156 2382 3155 3444 4329 5006 6154 Size: 543 7 226 773 289 885 677 1148 Cuts at: 6154 6399 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 885 773 677 543 289 245 226 7 NarI GG'CG_CC Cuts at: 2160 2336 2160 Size: 176 6737 NcoI C'CATG_G Cuts at: 513 1065 2004 3291 4176 513 Size: 552 939 1287 885 3250 Fragments arranged by size: 3250 1287 939 885 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6913 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6913 NotI GC'GGCC_GC Cuts at: 4360 4360 Size: 6913 NspI r_CATG'y Cuts at: 1132 1229 2953 3334 4271 5816 1132 Size: 97 1724 381 937 1545 2229 Fragments arranged by size: 2229 1724 1545 937 381 97 PasI CC'CwG_GG Cuts at: 1348 3989 1348 Size: 2641 4272 PciI A'CATG_T Cuts at: 5812 5812 Size: 6913 PflMI CCAn_nnn'nTGG Cuts at: 1108 3066 3308 3400 3636 1108 Size: 1958 242 92 236 4385 Fragments arranged by size: 4385 1958 242 236 92 PfoI T'CCnGG_A Cuts at: 3321 3321 Size: 6913 PmeI GTTT'AAAC Cuts at: 4217 4217 Size: 6913 PmlI CAC'GTG Cuts at: 3400 3400 Size: 6913 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4982 5014 6495 6527 4982 Size: 32 1481 32 5368 Fragments arranged by size: 5368 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6913 PsiI TTA'TAA Cuts at: 4483 4483 Size: 6913 PspOMI G'GGCC_C Cuts at: 3834 3934 4104 3834 Size: 100 170 6643 Fragments arranged by size: 6643 170 100 PstI C_TGCA'G Cuts at: 830 2148 4265 830 Size: 1318 2117 3478 Fragments arranged by size: 3478 2117 1318 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6913 SacI G_AGCT'C Cuts at: 2588 2588 Size: 6913 SacII CC_GC'GG Cuts at: 2157 3156 2157 Size: 999 5914 SalI G'TCGA_C Cuts at: 1936 3784 4255 1936 Size: 1848 471 4594 Fragments arranged by size: 4594 1848 471 SbfI CC_TGCA'GG Cuts at: 4265 4265 Size: 6913 ScaI AGT'ACT Cuts at: 2168 5161 6873 2168 Size: 2993 1712 2208 Fragments arranged by size: 2993 2208 1712 SexAI A'CCwGG_T Cuts at: 2085 2085 Size: 6913 SfcI C'TryA_G Cuts at: 826 1045 2144 3685 4261 5396 6077 6268 826 Size: 219 1099 1541 576 1135 681 191 1471 Fragments arranged by size: 1541 1471 1135 1099 681 576 219 191 SfoI GGC'GCC Cuts at: 2161 2337 2161 Size: 176 6737 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6913 SgrAI Cr'CCGG_yG Cuts at: 1700 2712 1700 Size: 1012 5901 SmaI CCC'GGG Cuts at: 1872 4240 1872 Size: 2368 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2395 2551 2702 3338 5017 5918 Size: 197 926 452 156 151 636 1679 901 Cuts at: 5918 6180 820 Size: 262 1553 Fragments arranged by size: 1679 1553 926 901 636 452 262 197 156 151 SnaBI TAC'GTA Cuts at: 493 4704 493 Size: 4211 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6913 SphI G_CATG'C Cuts at: 1132 4271 1132 Size: 3139 3774 SspI AAT'ATT Cuts at: 5 52 4837 5 Size: 47 4785 2081 Fragments arranged by size: 4785 2081 47 StuI AGG'CCT Cuts at: 1517 2401 3086 3462 1517 Size: 884 685 376 4968 Fragments arranged by size: 4968 884 685 376 StyI C'CwwG_G Cuts at: 513 1065 2004 3285 3291 4176 4352 513 Size: 552 939 1281 6 885 176 3074 Fragments arranged by size: 3074 1281 939 885 552 176 6 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3130 4002 4940 4957 5110 5295 5740 Size: 1255 1550 872 938 17 153 185 445 Cuts at: 5740 325 Size: 1498 Fragments arranged by size: 1550 1498 1255 938 872 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2166 2826 5159 Size: 274 80 33 51 1443 189 660 2333 Cuts at: 5159 6871 96 Size: 1712 138 Fragments arranged by size: 2333 1712 1443 660 274 189 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3317 3349 4062 4094 6873 6905 3317 Size: 32 713 32 2779 32 3325 Fragments arranged by size: 3325 2779 713 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6913 XbaI T'CTAG_A Cuts at: 4249 4249 Size: 6913 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2540 2540 Size: 6913 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6913 XmaI C'CCGG_G Cuts at: 1870 4238 1870 Size: 2368 4545 XmnI GAAnn'nnTTC Cuts at: 5042 5042 Size: 6913 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2754 4721 276 Size: 53 83 186 936 1220 1967 2468 Fragments arranged by size: 2468 1967 1220 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PstI PvuI SacI SacII SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BfrBI BsiWI BstZ17I EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PpuMI PspXI PsrI PvuII RsrII SanDI SapI SfiI SrfI SwaI