Restriction map for pFN21AB5706
 (Linear) (Six-base) MAPSORT of: KIBB5706.seq  Check: 5264  from: 1  to: 2151



KIBB5706.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:16  ..

AccI GT'mk_AC

Cuts at:      0    1500    2151 
   Size:       1500     651
 
AflIII A'CryG_T

Cuts at:      0     651    2151 
   Size:        651    1500
 
AlwNI CAG_nnn'CTG

Cuts at:      0     200     849    2151 
   Size:        200     649    1302

  Fragments arranged by size: 

                1302     649     200
 
ApaI G_GGCC'C

Cuts at:      0    1458    1989    2151 
   Size:       1458     531     162

  Fragments arranged by size: 

                1458     531     162
 
ApoI r'AATT_y

Cuts at:      0    1147    1922    2151 
   Size:       1147     775     229

  Fragments arranged by size: 

                1147     775     229
 
AvaI C'yCGr_G

Cuts at:      0    1388    2151 
   Size:       1388     763
 
BanI G'GyrC_C

Cuts at:      0     708    2151 
   Size:        708    1443
 
BanII G_rGCy'C

Cuts at:      0    1458    1989    2151 
   Size:       1458     531     162

  Fragments arranged by size: 

                1458     531     162
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     160    2151 
   Size:        160    1991
 
BclI T'GATC_A

Cuts at:      0     539    2151 
   Size:        539    1612
 
BlpI GC'TnA_GC

Cuts at:      0    1655    2151 
   Size:       1655     496
 
Bme1580I G_kGCm'C

Cuts at:      0    1458    1989    2151 
   Size:       1458     531     162

  Fragments arranged by size: 

                1458     531     162
 
BmgBI CAC'GTC

Cuts at:      0     652    2151 
   Size:        652    1499
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     171     179     888    1675    1825    2151 
   Size:        171       8     709     787     150     326

  Fragments arranged by size: 

                 787     709     326     171     150       8
 
BmtI G_CTAG'C

Cuts at:      0    1663    1999    2151 
   Size:       1663     336     152

  Fragments arranged by size: 

                1663     336     152
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     803    2151 
   Size:        803    1348
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     579    1463    1781    2151 
   Size:        579     884     318     370

  Fragments arranged by size: 

                 884     579     370     318
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     353     713     922    2151 
   Size:        353     360     209    1229

  Fragments arranged by size: 

                1229     360     353     209
 
BsaBI GATnn'nnATC

Cuts at:      0    1598    2151 
   Size:       1598     553
 
BsaWI w'CCGG_w

Cuts at:      0    1674    2151 
   Size:       1674     477
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    2084    2114    2151 
   Size:       2084      30      37

  Fragments arranged by size: 

                2084      37      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     704     716    1862    2151 
   Size:        704      12    1146     289

  Fragments arranged by size: 

                1146     704     289      12
 
BseYI C'CCAG_C

Cuts at:      0     858    1302    2151 
   Size:        858     444     849

  Fragments arranged by size: 

                 858     849     444
 
BsiHKAI G_wGCw'C

Cuts at:      0    2130    2151 
   Size:       2130      21
 
BsmI GAATG_Cn'

Cuts at:      0     107     648    1531    2151 
   Size:        107     541     883     620

  Fragments arranged by size: 

                 883     620     541     107
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0       2    2151 
   Size:          2    2149
 
Bsp1286I G_dGCh'C

Cuts at:      0    1458    1989    2130    2151 
   Size:       1458     531     141      21

  Fragments arranged by size: 

                1458     531     141      21
 
BspEI T'CCGG_A

Cuts at:      0    1674    2151 
   Size:       1674     477
 
BspHI T'CATG_A

Cuts at:      0    2112    2151 
   Size:       2112      39
 
BsrGI T'GTAC_A

Cuts at:      0     209    1899    2151 
   Size:        209    1690     252

  Fragments arranged by size: 

                1690     252     209
 
BstYI r'GATC_y

Cuts at:      0     469    2151 
   Size:        469    1682
 
BstZ17I GTA'TAC

Cuts at:      0    1501    2151 
   Size:       1501     650
 
BtgI C'CryG_G

Cuts at:      0      17    2018    2151 
   Size:         17    2001     133

  Fragments arranged by size: 

                2001     133      17
 
DraI TTT'AAA

Cuts at:      0    1194    2151 
   Size:       1194     957
 
EarI CTCTTCn'nnn_

Cuts at:      0      71     501     575     794    1043    1535    2151 
   Size:         71     430      74     219     249     492     616

  Fragments arranged by size: 

                 616     492     430     249     219      74      71
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     579    1463    1781    2151 
   Size:        579     884     318     370

  Fragments arranged by size: 

                 884     579     370     318
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     277    1372    2151 
   Size:        277    1095     779

  Fragments arranged by size: 

                1095     779     277
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1891    1985    1986    2151 
   Size:       1891      94       1     165

  Fragments arranged by size: 

                1891     165      94       1
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     606     638    2084    2116    2151 
   Size:        606      32    1446      32      35

  Fragments arranged by size: 

                1446     606      35      32      32
 
MfeI C'AATT_G

Cuts at:      0    1103    2151 
   Size:       1103    1048
 
MslI CAynn'nnrTG

Cuts at:      0    1232    1370    2151 
   Size:       1232     138     781

  Fragments arranged by size: 

                1232     781     138
 
MspA1I CmG'CkG

Cuts at:      0      31     499    1846    2151 
   Size:         31     468    1347     305

  Fragments arranged by size: 

                1347     468     305      31
 
NdeI CA'TA_TG

Cuts at:      0      87     318     375    1035    2151 
   Size:         87     231      57     660    1116

  Fragments arranged by size: 

                1116     660     231      87      57
 
NheI G'CTAG_C

Cuts at:      0    1659    1995    2151 
   Size:       1659     336     156

  Fragments arranged by size: 

                1659     336     156
 
PpuMI rG'GwC_Cy

Cuts at:      0    1891    2151 
   Size:       1891     260
 
PsiI TTA'TAA

Cuts at:      0     814    1637    2151 
   Size:        814     823     514

  Fragments arranged by size: 

                 823     814     514
 
PspOMI G'GGCC_C

Cuts at:      0    1454    1985    2151 
   Size:       1454     531     166

  Fragments arranged by size: 

                1454     531     166
 
PstI C_TGCA'G

Cuts at:      0    1245    1845    2151 
   Size:       1245     600     306

  Fragments arranged by size: 

                1245     600     306
 
PvuII CAG'CTG

Cuts at:      0      31     499    1846    2151 
   Size:         31     468    1347     305

  Fragments arranged by size: 

                1347     468     305      31
 
ScaI AGT'ACT

Cuts at:      0    2036    2151 
   Size:       2036     115
 
SexAI A'CCwGG_T

Cuts at:      0    1815    2151 
   Size:       1815     336
 
SfcI C'TryA_G

Cuts at:      0     137     464    1241    1263    1697    1841    2000    2151 
   Size:        137     327     777      22     434     144     159     151

  Fragments arranged by size: 

                 777     434     327     159     151     144     137      22
            
 
SmlI C'TyrA_G

Cuts at:      0     368     728     901    2151 
   Size:        368     360     173    1250

  Fragments arranged by size: 

                1250     368     360     173
 
SpeI A'CTAG_T

Cuts at:      0    1569    2151 
   Size:       1569     582
 
SspI AAT'ATT

Cuts at:      0     594    1258    1482    2151 
   Size:        594     664     224     669

  Fragments arranged by size: 

                 669     664     594     224
 
StyI C'CwwG_G

Cuts at:      0     332    1124    2042    2151 
   Size:        332     792     918     109

  Fragments arranged by size: 

                 918     792     332     109
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     417    2151 
   Size:        417    1734
 
TatI w'GTAC_w

Cuts at:      0     209    1899    2034    2151 
   Size:        209    1690     135     117

  Fragments arranged by size: 

                1690     209     135     117
 
XmnI GAAnn'nnTTC

Cuts at:      0    1073    2151 
   Size:       1073    1078
 

 Enzymes that do cut:

     AccI   AflIII    AlwNI     ApaI     ApoI     AvaI     BanI    BanII
     BbsI     BclI     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI
     BpmI    BpuEI    BsaBI    BsaWI    BsaXI    BseRI    BseYI  BsiHKAI
     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BsrGI    BstYI  BstZ17I
     BtgI     DraI     EarI  Eco57MI    EcoNI EcoO109I    Hin4I     MfeI
     MslI   MspA1I     NdeI     NheI    PpuMI     PsiI   PspOMI     PstI
    PvuII     ScaI    SexAI     SfcI     SmlI     SpeI     SspI     StyI
    TaqII     TatI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI    AflII     AgeI
     AhdI     AleI     AlfI     AloI    ApaLI     AscI     AseI    AsiSI
    AvrII     BaeI    BamHI     BbeI    BbvCI     BcgI    BciVI    BfrBI
     BglI    BglII   Bpu10I     BsaI    BsaAI    BsaHI     BsgI    BsiEI
    BsiWI    BspMI    BsrBI    BsrDI    BsrFI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI   Bsu36I    BtgZI     BtsI     ClaI    CspCI
   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI    HaeII   HincII  HindIII     HpaI
     KasI     KpnI     MluI     MmeI     MscI     NaeI     NarI     NcoI
   NgoMIV     NotI     NruI     NsiI     NspI     PacI     PasI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI    PshAI    PspXI     PsrI
     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SphI     SrfI
     StuI     SwaI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI
     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com