Restriction map for pFN21AB9973
 (Linear) (Six-base) MAPSORT of: KIBB9973.seq  Check: 9355  from: 1  to: 1662



KIBB9973.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:26  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     149     778    1505    1662 
   Size:        149     629     727     157

  Fragments arranged by size: 

                 727     629     157     149
 
AccI GT'mk_AC

Cuts at:      0     209    1662 
   Size:        209    1453
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     279     376    1662 
   Size:        279      97    1286

  Fragments arranged by size: 

                1286     279      97
 
AflII C'TTAA_G

Cuts at:      0     244    1662 
   Size:        244    1418
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1318    1662 
   Size:       1318     344
 
AlwNI CAG_nnn'CTG

Cuts at:      0     150     159     435     720    1662 
   Size:        150       9     276     285     942

  Fragments arranged by size: 

                 942     285     276     150       9
 
ApaI G_GGCC'C

Cuts at:      0    1114    1662 
   Size:       1114     548
 
AvaI C'yCGr_G

Cuts at:      0     865    1290    1662 
   Size:        865     425     372

  Fragments arranged by size: 

                 865     425     372
 
BamHI G'GATC_C

Cuts at:      0     732    1459    1662 
   Size:        732     727     203

  Fragments arranged by size: 

                 732     727     203
 
BanI G'GyrC_C

Cuts at:      0      13    1503    1662 
   Size:         13    1490     159

  Fragments arranged by size: 

                1490     159      13
 
BanII G_rGCy'C

Cuts at:      0      52    1010    1114    1354    1662 
   Size:         52     958     104     240     308

  Fragments arranged by size: 

                 958     308     240     104      52
 
BbeI G_GCGC'C

Cuts at:      0      17    1662 
   Size:         17    1645
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1162    1482    1662 
   Size:       1162     320     180

  Fragments arranged by size: 

                1162     320     180
 
BbvCI CC'TCA_GC

Cuts at:      0    1237    1662 
   Size:       1237     425
 
BglI GCCn_nnn'nGGC

Cuts at:      0     652    1232    1662 
   Size:        652     580     430

  Fragments arranged by size: 

                 652     580     430
 
BlpI GC'TnA_GC

Cuts at:      0    1262    1662 
   Size:       1262     400
 
Bme1580I G_kGCm'C

Cuts at:      0     901    1114    1508    1662 
   Size:        901     213     394     154

  Fragments arranged by size: 

                 901     394     213     154
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     793    1502    1662 
   Size:        793     709     160

  Fragments arranged by size: 

                 793     709     160
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     711    1218    1479    1662 
   Size:        711     507     261     183

  Fragments arranged by size: 

                 711     507     261     183
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     412     457     519     947     960    1339    1449    1462 
   Size:        412      45      62     428      13     379     110      13

Cuts at:   1462    1662 
   Size:        200

  Fragments arranged by size: 

                 428     412     379     200     110      62      45      13
                  13
 
Bpu10I CC'TnA_GC

Cuts at:      0    1237    1662 
   Size:       1237     425
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     172     787    1178    1662 
   Size:        172     615     391     484

  Fragments arranged by size: 

                 615     484     391     172
 
BsaHI Gr'CG_yC

Cuts at:      0      14    1662 
   Size:         14    1648
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     169     199    1662 
   Size:        169      30    1463

  Fragments arranged by size: 

                1463     169      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     112    1278    1344    1362    1662 
   Size:        112    1166      66      18     300

  Fragments arranged by size: 

                1166     300     112      66      18
 
BseYI C'CCAG_C

Cuts at:      0      45    1018    1662 
   Size:         45     973     644

  Fragments arranged by size: 

                 973     644      45
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     349     706    1662 
   Size:        349     357     956

  Fragments arranged by size: 

                 956     357     349
 
BsiHKAI G_wGCw'C

Cuts at:      0     769    1336    1662 
   Size:        769     567     326

  Fragments arranged by size: 

                 769     567     326
 
BsmI GAATG_Cn'

Cuts at:      0     535    1662 
   Size:        535    1127
 
Bsp1286I G_dGCh'C

Cuts at:      0      52     769     901    1010    1114    1336    1354    1508 
   Size:         52     717     132     109     104     222      18     154

Cuts at:   1508    1662 
   Size:        154

  Fragments arranged by size: 

                 717     222     154     154     132     109     104      52
                  18
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     149     778    1505    1662 
   Size:        149     629     727     157

  Fragments arranged by size: 

                 727     629     157     149
 
BsrBI CCG'CTC

Cuts at:      0    1591    1662 
   Size:       1591      71
 
BsrDI GCAATG_nn'

Cuts at:      0     899    1662 
   Size:        899     763
 
BsrFI r'CCGG_y

Cuts at:      0    1074    1662 
   Size:       1074     588
 
BssSI C'ACGA_G

Cuts at:      0     183    1662 
   Size:        183    1479
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     150     509     720    1662 
   Size:        150     359     211     942

  Fragments arranged by size: 

                 942     359     211     150
 
BstEII G'GTnAC_C

Cuts at:      0     549    1662 
   Size:        549    1113
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     127    1662 
   Size:        127    1535
 
BstYI r'GATC_y

Cuts at:      0     228     732    1211    1459    1662 
   Size:        228     504     479     248     203

  Fragments arranged by size: 

                 504     479     248     228     203
 
BtgI C'CryG_G

Cuts at:      0     641    1662 
   Size:        641    1021
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     303    1662 
   Size:        303    1359
 
BtsI GCAGTG_nn'

Cuts at:      0     603     747    1219    1662 
   Size:        603     144     472     443

  Fragments arranged by size: 

                 603     472     443     144
 
DraIII CAC_nnn'GTG

Cuts at:      0     729    1662 
   Size:        729     933
 
EaeI y'GGCC_r

Cuts at:      0     754    1128    1529    1662 
   Size:        754     374     401     133

  Fragments arranged by size: 

                 754     401     374     133
 
EarI CTCTTCn'nnn_

Cuts at:      0     352     377    1662 
   Size:        352      25    1285

  Fragments arranged by size: 

                1285     352      25
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1581    1662 
   Size:       1581      81
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     279     376     412     457     519     947     960    1339 
   Size:        279      97      36      45      62     428      13     379

Cuts at:   1339    1449    1462    1662 
   Size:        110      13     200

  Fragments arranged by size: 

                 428     379     279     200     110      97      62      45
                  36      13      13
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     247     481    1101    1630    1662 
   Size:        247     234     620     529      32

  Fragments arranged by size: 

                 620     529     247     234      32
 
EcoO109I rG'GnC_Cy

Cuts at:      0     854     909    1111    1233    1397    1662 
   Size:        854      55     202     122     164     265

  Fragments arranged by size: 

                 854     265     202     164     122      55
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     381     471    1662 
   Size:        381      90    1191

  Fragments arranged by size: 

                1191     381      90
 
HaeII r_GCGC'y

Cuts at:      0      17      27    1662 
   Size:         17      10    1635

  Fragments arranged by size: 

                1635      17      10
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      88     120     153     185     592     624    1149    1153 
   Size:         88      32      33      32     407      32     525       4

Cuts at:   1153    1181    1185    1435    1467    1662 
   Size:         28       4     250      32     195

  Fragments arranged by size: 

                 525     407     250     195      88      33      32      32
                  32      32      28       4       4
 
KasI G'GCGC_C

Cuts at:      0      13    1662 
   Size:         13    1649
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      86    1515    1662 
   Size:         86    1429     147

  Fragments arranged by size: 

                1429     147      86
 
MscI TGG'CCA

Cuts at:      0    1130    1662 
   Size:       1130     532
 
MspA1I CmG'CkG

Cuts at:      0     156     333     753    1662 
   Size:        156     177     420     909

  Fragments arranged by size: 

                 909     420     177     156
 
NaeI GCC'GGC

Cuts at:      0    1076    1662 
   Size:       1076     586
 
NarI GG'CG_CC

Cuts at:      0      14    1662 
   Size:         14    1648
 
NcoI C'CATG_G

Cuts at:      0     641    1662 
   Size:        641    1021
 
NgoMIV G'CCGG_C

Cuts at:      0    1074    1662 
   Size:       1074     588
 
NspI r_CATG'y

Cuts at:      0     999    1662 
   Size:        999     663
 
PasI CC'CwG_GG

Cuts at:      0    1103    1230    1662 
   Size:       1103     127     432

  Fragments arranged by size: 

                1103     432     127
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      41     435     546    1500    1662 
   Size:         41     394     111     954     162

  Fragments arranged by size: 

                 954     394     162     111      41
 
PfoI T'CCnGG_A

Cuts at:      0    1478    1662 
   Size:       1478     184
 
PpuMI rG'GwC_Cy

Cuts at:      0     909    1662 
   Size:        909     753
 
PspOMI G'GGCC_C

Cuts at:      0    1110    1662 
   Size:       1110     552
 
PstI C_TGCA'G

Cuts at:      0     146     155     338    1662 
   Size:        146       9     183    1324

  Fragments arranged by size: 

                1324     183     146       9
 
PvuII CAG'CTG

Cuts at:      0     333    1662 
   Size:        333    1329
 
SanDI GG'GwC_CC

Cuts at:      0     909    1662 
   Size:        909     753
 
SapI GCTCTTCn'nnn_

Cuts at:      0     352     377    1662 
   Size:        352      25    1285

  Fragments arranged by size: 

                1285     352      25
 
ScaI AGT'ACT

Cuts at:      0     112    1662 
   Size:        112    1550
 
SfcI C'TryA_G

Cuts at:      0     114     142     151     334    1551    1662 
   Size:        114      28       9     183    1217     111

  Fragments arranged by size: 

                1217     183     114     111      28       9
 
SfoI GGC'GCC

Cuts at:      0      15    1662 
   Size:         15    1647
 
SmaI CCC'GGG

Cuts at:      0     867    1662 
   Size:        867     795
 
SmlI C'TyrA_G

Cuts at:      0     244    1662 
   Size:        244    1418
 
SphI G_CATG'C

Cuts at:      0     999    1662 
   Size:        999     663
 
SspI AAT'ATT

Cuts at:      0     495    1662 
   Size:        495    1167
 
StuI AGG'CCT

Cuts at:      0     397    1541    1662 
   Size:        397    1144     121

  Fragments arranged by size: 

                1144     397     121
 
StyI C'CwwG_G

Cuts at:      0     371     641     954    1308    1601    1662 
   Size:        371     270     313     354     293      61

  Fragments arranged by size: 

                 371     354     313     293     270      61
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     774    1662 
   Size:        774     888
 
TatI w'GTAC_w

Cuts at:      0     110    1662 
   Size:        110    1552
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     169     201     717     749    1662 
   Size:        169      32     516      32     913

  Fragments arranged by size: 

                 913     516     169      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      42     543     648    1515    1662 
   Size:         42     501     105     867     147

  Fragments arranged by size: 

                 867     501     147     105      42
 
XmaI C'CCGG_G

Cuts at:      0     865    1662 
   Size:        865     797
 
XmnI GAAnn'nnTTC

Cuts at:      0     496    1662 
   Size:        496    1166
 

 Enzymes that do cut:

     AarI     AccI     AcuI    AflII     AlfI    AlwNI     ApaI     AvaI
    BamHI     BanI    BanII     BbeI     BbsI    BbvCI     BglI     BlpI
 Bme1580I     BmrI     BplI     BpmI   Bpu10I     BsaI    BsaHI    BsaXI
    BseRI    BseYI     BsgI  BsiHKAI     BsmI Bsp1286I    BspMI    BsrBI
    BsrDI    BsrFI    BssSI   BstAPI   BstEII    BstXI    BstYI     BtgI
    BtgZI     BtsI   DraIII     EaeI     EarI     EciI  Eco57MI    EcoNI
 EcoO109I     FalI    HaeII    Hin4I     KasI     MmeI     MscI   MspA1I
     NaeI     NarI     NcoI   NgoMIV     NspI     PasI    PflMI     PfoI
    PpuMI   PspOMI     PstI    PvuII    SanDI     SapI     ScaI     SfcI
     SfoI     SmaI     SmlI     SphI     SspI     StuI     StyI    TaqII
     TatI     TstI     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AfeI   AflIII     AgeI     AhdI     AleI
     AloI    ApaLI     ApoI     AscI     AseI    AsiSI    AvrII     BaeI
     BcgI    BciVI     BclI    BfrBI    BglII    BmgBI     BmtI    BpuEI
    BsaAI    BsaBI    BsaWI    BsiEI    BsiWI    BsmBI    BspEI    BspHI
    BsrGI   BssHII    BstBI  BstZ17I   Bsu36I     ClaI    CspCI     DraI
     DrdI     EagI  EcoICRI    EcoRI    EcoRV     FseI     FspI    FspAI
   HincII  HindIII     HpaI     KpnI     MfeI     MluI     MslI     NdeI
     NheI     NotI     NruI     NsiI     PacI     PciI     PmeI     PmlI
     PpiI    PshAI     PsiI    PspXI     PsrI     PvuI    RsrII     SacI
    SacII     SalI     SbfI    SexAI     SfiI     SgfI    SgrAI    SnaBI
     SpeI     SrfI     SwaI  Tth111I     XbaI     XhoI     ZraI
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