Restriction map for
pFN21AB7992
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# NO CUT ENZYMES ( 140 enzymes) #
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AatII AccI AccII AccIII AclI
AcyI AflII AflIII AgeI Alw44I
Aor51HI ApaLI AscI AseI AsnI
AspI AviII AvrII BalI BamHI
BbeI BbrPI BbsI BciVI BclI
BfrI BglI BlnI BlpI Bpu1102I
BpuAI BsaAI BsaBI BsaHI BsaWI
BseAI BsiEI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstEII BstPI BstUI
BstXI BstZ17I Cac8I CelII CfoI
Cfr10I ClaI CpoI DraI DraIII
DrdI EaeI EagI EclXI Eco47III
Eco52I EcoO65I EcoRV EcoT22I FbaI
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I HincII HindII HpaI
KasI KspI MaeII MamI MfeI
MluI MluNI MroI MscI MseI
MslI MunI MvnI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI SbfI SexAI
SfiI SfoI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
Tru9I Tth111I Van91I XbaI XhoI
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# ONE CUT ENZYMES ( 37 enzymes) #
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Acc65I 757 GGTACC G^GTACC
AhdI 267 GACTCCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 25 GGGCCC GGGCC^C
Asp718I 757 GGTACC G^GTACC
AspEI 267 GACTCCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 278 CCCGGG C^[CT]CG[GA]G
BanI 757 GGTACC G^G[CT][GA]CC
BbvCI 321 GCTGAGG CCTCAGC(-5/-2)
BcgI 301 CGAGTACTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 227 AGATCT A^GATCT
BsoBI 278 CCCGGG C^[CT]CG[GA]G
BsrDI 665 CATTGC GCAATG(2/0)
BsrI 218 CCAGT ACTGG(1/-1)
BstBI 1048 TTCGAA TT^CGAA
DsaI 535 CCATGG C^C[GA][CT]GG
Eam1105I 267 GACTCCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 390 CCTGCCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI 417 GCGGG CCCGC(4/6)
HapII 279 CCGG C^CGG
HindIII 1238 AAGCTT A^AGCTT
HpaII 279 CCGG C^CGG
HphI 426 TCACC GGTGA(8/7)
KpnI 757 GGTACC GGTAC^C
MspI 279 CCGG C^CGG
NcoI 535 CCATGG C^CATGG
NspV 1048 TTCGAA TT^CGAA
PleI 267 GACTC GAGTC(4/5)
PpuMI 497 AGGTCCC [GA]G^G[AT]CC[CT]
SapI 529 GAAGAGC GCTCTTC(1/4)
ScaI 303 AGTACT AGT^ACT
SfuI 1048 TTCGAA TT^CGAA
SmaI 278 CCCGGG CCC^GGG
SmlI 288 CTTGAG C^T[CT][GA]AG
TaqI 1049 TCGA T^CGA
TthHB8I 1049 TCGA T^CGA
XmaCI 278 CCCGGG C^CCGGG
XmaI 278 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 27 enzymes) #
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AlwI 351 GATCC GGATC(4/5)
661 GGATC
AocI 394 CCTGAGG CC^T[ACGT]AGG
595 CCTAAGG
BcnI 278 CCCGG CC^[GC]GG
279 CCGGG
BfaI 67 CTAG C^TAG
77 CTAG
BsaI 190 GAGACC GGTCTC(1/5)
601 GAGACC
BseRI 397 GAGGAG GAGGAG(10/8)
442 GAGGAG
BspMI 519 ACCTGC ACCTGC(4/8)
495 GCAGGT
BstYI 227 AGATCT [GA]^GATC[CT]
350 AGATCC
Bsu36I 394 CCTGAGG CC^T[ACGT]AGG
595 CCTAAGG
EarI 166 GAAGAG CTCTTC(1/4)
529 GAAGAG
Eco81I 394 CCTGAGG CC^T[ACGT]AGG
595 CCTAAGG
EcoRI 1025 GAATTC G^AATTC
1193 GAATTC
EcoT14I 535 CCATGG C^C[AT][AT]GG
650 CCAAGG
Ksp632I 166 GAAGAG CTCTTC(1/4)
529 GAAGAG
MaeI 67 CTAG C^TAG
77 CTAG
MflI 227 AGATCT [GA]^GATC[CT]
350 AGATCC
MspA1I 42 CAGCTG C[AC]G^C[GT]G
101 CAGCTG
MwoI 393 GCCTGAGGAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
565 GCCCAGGAAGC
NciI 278 CCCGG CC^[GC]GG
279 CCGGG
PstI 59 CTGCAG CTGCA^G
769 CTGCAG
PvuII 42 CAGCTG CAG^CTG
101 CAGCTG
SfcI 59 CTGCAG C^T[GA][CT]AG
769 CTGCAG
StyI 535 CCATGG C^C[AT][AT]GG
650 CCAAGG
Tsp45I 451 GTGAC ^GT[GC]AC
974 GTGAC
TspRI 575 CACTG CA[GC]TG[ACGT][ACGT]^
1200 CAGTG
XcmI 261 CCAGGAGACTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
680 CCATTACTGAAGTGG
XhoII 227 AGATCT [GA]^GATC[CT]
350 AGATCC
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# 3 OR MORE CUT ENZYMES ( 54 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwNI
ApoI Asp700I AspHI AvaII BanII
BbvI BmyI BpmI BsaJI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BssKI BstF5I BstNI Cfr13I
DdeI DpnII DraII EcoO109I EcoRII
Fnu4HI FokI HaeIII HinfI ItaI
MaeIII MboI MboII MnlI MvaI
NdeII NlaIII NlaIV RsaI SacI
Sau3AI Sau96I ScrFI SfaNI StuI
TfiI TseI Tsp509I XmnI