(Circular) (Six-base) MAPSORT of: pFN21AB7987.seq Check: 8580 from: 1 to: 6028 pFN21AB7987 6028 bp With 182 enzymes: SgfI * April 15, 2010 09:28 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3838 278 Size: 53 83 186 936 2302 2468 Fragments arranged by size: 2468 2302 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3371 1937 Size: 1434 4594 Acc65I G'GTAC_C Cuts at: 3147 3349 3147 Size: 202 5826 AclI AA'CG_TT Cuts at: 1563 4155 4528 1563 Size: 2592 373 3063 Fragments arranged by size: 3063 2592 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2159 2861 2878 2936 3065 4091 5475 5743 Size: 527 702 17 58 129 1026 1384 268 Cuts at: 5743 1632 Size: 1917 Fragments arranged by size: 1917 1384 1026 702 527 268 129 58 17 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5831 AflIII A'CryG_T Cuts at: 1203 4927 5904 1203 Size: 3724 977 1327 Fragments arranged by size: 3724 1327 977 AgeI A'CCGG_T Cuts at: 4859 4859 Size: 6028 AhdI GACnn_n'nnGTC Cuts at: 2173 2326 4757 2173 Size: 153 2431 3444 Fragments arranged by size: 3444 2431 153 AleI CACnn'nnGTG Cuts at: 1166 3315 1166 Size: 2149 3879 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6028 AlwNI CAG_nnn'CTG Cuts at: 2452 2680 2697 2839 3194 3771 5343 2452 Size: 228 17 142 355 577 1572 3137 Fragments arranged by size: 3137 1572 577 355 228 142 17 ApaI G_GGCC'C Cuts at: 2073 2224 2912 2073 Size: 151 688 5189 Fragments arranged by size: 5189 688 151 ApaLI G'TGCA_C Cuts at: 2876 3113 4085 5241 2876 Size: 237 972 1156 3663 Fragments arranged by size: 3663 1156 972 237 ApoI r'AATT_y Cuts at: 1493 3337 3563 3715 5824 1493 Size: 1844 226 152 2109 1697 Fragments arranged by size: 2109 1844 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4582 160 Size: 872 3550 1606 Fragments arranged by size: 3550 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6028 AvaI C'yCGr_G Cuts at: 1870 1943 2799 3293 3353 1870 Size: 73 856 494 60 4545 Fragments arranged by size: 4545 856 494 73 60 BamHI G'GATC_C Cuts at: 2517 2853 3030 3358 3725 2517 Size: 336 177 328 367 4820 Fragments arranged by size: 4820 367 336 328 177 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3147 3349 4804 618 Size: 325 217 627 1360 202 1455 1842 Fragments arranged by size: 1842 1455 1360 627 325 217 202 BanII G_rGCy'C Cuts at: 1391 2073 2224 2912 3126 3347 1391 Size: 682 151 688 214 221 4072 Fragments arranged by size: 4072 688 682 221 214 151 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5057 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4219 4253 1903 Size: 34 2282 34 3678 Fragments arranged by size: 3678 2282 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3920 5130 3920 Size: 1210 4818 BclI T'GATC_A Cuts at: 1548 1548 Size: 6028 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2156 4639 136 Size: 107 122 71 631 605 484 2483 1525 Fragments arranged by size: 2483 1525 631 605 484 122 107 71 BglII A'GATC_T Cuts at: 6023 6023 Size: 6028 BlpI GC'TnA_GC Cuts at: 3445 3445 Size: 6028 Bme1580I G_kGCm'C Cuts at: 1790 2073 2224 2367 2465 2880 2912 3117 4089 Size: 283 151 143 98 415 32 205 972 Cuts at: 4089 5245 1790 Size: 1156 2573 Fragments arranged by size: 2573 1156 972 415 283 205 151 143 98 32 BmgBI CAC'GTC Cuts at: 1318 2146 1318 Size: 828 5200 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3097 3759 4717 Size: 416 53 83 399 24 1680 662 958 Cuts at: 4717 442 Size: 1753 Fragments arranged by size: 1753 1680 958 662 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6028 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2282 4688 1239 Size: 1043 2406 2579 Fragments arranged by size: 2579 2406 1043 Bpu10I CC'TnA_GC Cuts at: 3120 3120 Size: 6028 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4153 5018 5316 4153 Size: 865 298 4865 Fragments arranged by size: 4865 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2522 3300 4691 882 Size: 617 1023 778 1391 2219 Fragments arranged by size: 2219 1391 1023 778 617 BsaAI yAC'GTr Cuts at: 493 1204 3819 493 Size: 711 2615 2702 Fragments arranged by size: 2702 2615 711 BsaBI GATnn'nnATC Cuts at: 2132 6022 2132 Size: 3890 2138 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2046 2372 2501 3835 Size: 53 83 186 936 513 326 129 1334 Cuts at: 3835 4217 4852 275 Size: 382 635 1451 Fragments arranged by size: 1451 1334 936 635 513 382 326 186 129 83 53 BsaWI w'CCGG_w Cuts at: 2292 4461 4859 5133 5280 5691 2292 Size: 2169 398 274 147 411 2629 Fragments arranged by size: 2629 2169 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5803 Fragments arranged by size: 5803 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2396 3005 1186 Size: 1210 609 4209 Fragments arranged by size: 4209 1210 609 BseYI C'CCAG_C Cuts at: 2647 3187 3212 5231 5727 2647 Size: 540 25 2019 496 2948 Fragments arranged by size: 2948 2019 540 496 25 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2060 2101 2426 2693 2923 1163 Size: 573 324 41 325 267 230 4268 Fragments arranged by size: 4268 573 325 324 267 230 41 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2334 2391 3478 4239 5267 1540 Size: 275 186 333 57 1087 761 1028 2301 Fragments arranged by size: 2301 1087 1028 761 333 275 186 57 BsiHKAI G_wGCw'C Cuts at: 2607 2730 2880 3117 3126 4089 4174 5245 2607 Size: 123 150 237 9 963 85 1071 3390 Fragments arranged by size: 3390 1071 963 237 150 123 85 9 BsmI GAATG_Cn' Cuts at: 3539 3632 3539 Size: 93 5935 BsmBI CGTCTCn'nnnn_ Cuts at: 2378 2378 Size: 6028 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2073 2224 2367 2465 2607 2730 2880 Size: 399 283 151 143 98 142 123 150 Cuts at: 2880 2912 3117 3126 3347 4089 4174 5245 1391 Size: 32 205 9 221 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 283 221 205 151 150 143 142 123 98 85 32 9 BspEI T'CCGG_A Cuts at: 2292 5691 2292 Size: 3399 2629 BspHI T'CATG_A Cuts at: 2061 3917 5647 2061 Size: 1856 1730 2442 Fragments arranged by size: 2442 1856 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2688 3135 3383 844 Size: 1844 447 248 3489 Fragments arranged by size: 3489 1844 447 248 BsrBI CCG'CTC Cuts at: 1394 1823 2641 3915 1394 Size: 429 818 1274 3507 Fragments arranged by size: 3507 1274 818 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2096 2346 4523 4697 65 Size: 1183 848 250 2177 174 1396 Fragments arranged by size: 2177 1396 1183 848 250 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2424 4672 4859 1700 Size: 110 614 2248 187 2869 Fragments arranged by size: 2869 2248 614 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6028 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6028 BssSI C'ACGA_G Cuts at: 2466 2879 4088 5100 2466 Size: 413 1209 1012 3394 Fragments arranged by size: 3394 1209 1012 413 BstAPI GCAn_nnn'nTGC Cuts at: 794 2680 794 Size: 1886 4142 BstBI TT'CG_AA Cuts at: 4832 4832 Size: 6028 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6028 BstYI r'GATC_y Cuts at: 1972 2187 2487 2517 2853 3006 3030 3358 3725 Size: 215 300 30 336 153 24 328 367 Cuts at: 3725 4110 4127 5579 5665 5677 6023 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 385 367 346 336 328 300 215 153 86 30 24 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2081 2367 2521 2857 3232 513 Size: 552 939 77 286 154 336 375 3309 Fragments arranged by size: 3309 939 552 375 336 286 154 77 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5289 Fragments arranged by size: 5289 683 56 BtsI GCAGTG_nn' Cuts at: 2308 2574 2710 3544 4338 4358 2308 Size: 266 136 834 794 20 3978 Fragments arranged by size: 3978 834 794 266 136 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6028 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5993 DraI TTT'AAA Cuts at: 3332 3332 Size: 6028 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2756 3107 5035 809 Size: 507 1440 351 1928 1802 Fragments arranged by size: 1928 1802 1440 507 351 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2107 2148 2331 Size: 54 1425 50 275 18 277 41 183 Cuts at: 2331 2388 3277 3475 4364 5993 8 Size: 57 889 198 889 1629 43 Fragments arranged by size: 1629 1425 889 889 277 275 198 183 57 54 50 43 41 18 EagI C'GGCC_G Cuts at: 1537 1812 2331 2388 3475 1537 Size: 275 519 57 1087 4090 Fragments arranged by size: 4090 1087 519 275 57 EarI CTCTTCn'nnn_ Cuts at: 1353 2612 2690 3958 1353 Size: 1259 78 1268 3423 Fragments arranged by size: 3423 1268 1259 78 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3314 4615 4989 5135 387 Size: 1064 138 1725 1301 374 146 1280 Fragments arranged by size: 1725 1301 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3124 3124 Size: 6028 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2159 2282 2861 2878 2936 3065 4091 Size: 393 527 123 579 17 58 129 1026 Cuts at: 4091 4688 5475 5743 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1026 787 597 579 527 393 268 129 123 58 17 EcoNI CCTnn'n_nnAGG Cuts at: 2207 2843 3142 2207 Size: 636 299 5093 Fragments arranged by size: 5093 636 299 EcoO109I rG'GnC_Cy Cuts at: 2032 2070 2200 2220 2221 2257 2677 2778 2836 Size: 38 130 20 1 36 420 101 58 Cuts at: 2836 2908 2909 2032 Size: 72 1 5151 Fragments arranged by size: 5151 420 130 101 72 58 38 36 20 1 1 EcoRI G'AATT_C Cuts at: 3337 3715 3337 Size: 378 5650 FspI TGC'GCA Cuts at: 4534 4534 Size: 6028 HaeII r_GCGC'y Cuts at: 1678 2453 2511 2576 2648 2828 3219 5175 1678 Size: 775 58 65 72 180 391 1956 2531 Fragments arranged by size: 2531 1956 775 391 180 72 65 58 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2043 2075 2159 2191 2312 Size: 32 135 32 471 32 84 32 121 Cuts at: 2312 2344 2486 2518 2957 2989 4669 4701 4743 Size: 32 142 32 439 32 1680 32 42 Cuts at: 4743 4775 1373 Size: 32 2626 Fragments arranged by size: 2626 1680 471 439 142 135 121 84 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3058 3372 3618 669 Size: 988 281 1120 314 246 3079 Fragments arranged by size: 3079 1120 988 314 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6028 HpaI GTT'AAC Cuts at: 3618 3618 Size: 6028 KpnI G_GTAC'C Cuts at: 3151 3353 3151 Size: 202 5826 MfeI C'AATT_G Cuts at: 3627 3627 Size: 6028 MluI A'CGCG_T Cuts at: 5904 5904 Size: 6028 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2074 2607 2865 5142 5326 1305 Size: 769 533 258 2277 184 2007 Fragments arranged by size: 2277 2007 769 533 258 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2150 3279 10 Size: 54 1425 343 318 1129 2759 Fragments arranged by size: 2759 1425 1129 343 318 54 MslI CAynn'nnrTG Cuts at: 518 1166 2981 3315 3986 4345 4504 518 Size: 648 1815 334 671 359 159 2042 Fragments arranged by size: 2042 1815 671 648 359 334 159 MspA1I CmG'CkG Cuts at: 1606 2083 2369 2523 2859 3191 3444 4121 5269 Size: 477 286 154 336 332 253 677 1148 Cuts at: 5269 5514 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 477 336 332 286 253 245 154 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4537 Fragments arranged by size: 4537 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6028 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6028 NotI GC'GGCC_GC Cuts at: 3475 3475 Size: 6028 NspI r_CATG'y Cuts at: 1132 1229 3386 4931 1132 Size: 97 2157 1545 2229 Fragments arranged by size: 2229 2157 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6028 PciI A'CATG_T Cuts at: 4927 4927 Size: 6028 PflMI CCAn_nnn'nTGG Cuts at: 1108 3087 1108 Size: 1979 4049 PmeI GTTT'AAAC Cuts at: 3332 3332 Size: 6028 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4097 4129 5610 5642 4097 Size: 32 1481 32 4483 Fragments arranged by size: 4483 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2200 2257 2677 2200 Size: 57 420 5551 Fragments arranged by size: 5551 420 57 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6028 PsiI TTA'TAA Cuts at: 3598 3598 Size: 6028 PspOMI G'GGCC_C Cuts at: 2069 2220 2908 2069 Size: 151 688 5189 Fragments arranged by size: 5189 688 151 PstI C_TGCA'G Cuts at: 830 2658 2676 3380 830 Size: 1828 18 704 3478 Fragments arranged by size: 3478 1828 704 18 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6028 PvuII CAG'CTG Cuts at: 3191 3191 Size: 6028 SacI G_AGCT'C Cuts at: 3126 3126 Size: 6028 SacII CC_GC'GG Cuts at: 2084 2370 2524 2860 2084 Size: 286 154 336 5252 Fragments arranged by size: 5252 336 286 154 SalI G'TCGA_C Cuts at: 1936 3370 1936 Size: 1434 4594 SapI GCTCTTCn'nnn_ Cuts at: 2612 2690 2612 Size: 78 5950 SbfI CC_TGCA'GG Cuts at: 2676 3380 2676 Size: 704 5324 ScaI AGT'ACT Cuts at: 4276 5988 4276 Size: 1712 4316 SfcI C'TryA_G Cuts at: 826 1045 2654 2672 3139 3376 4511 5192 5383 Size: 219 1609 18 467 237 1135 681 191 Cuts at: 5383 826 Size: 1471 Fragments arranged by size: 1609 1471 1135 681 467 237 219 191 18 SfiI GGCCn_nnn'nGGCC Cuts at: 2156 2156 Size: 6028 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6028 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6028 SmaI CCC'GGG Cuts at: 1872 2801 3355 1872 Size: 929 554 4545 Fragments arranged by size: 4545 929 554 SmlI C'TyrA_G Cuts at: 820 1017 1943 4132 5033 5295 820 Size: 197 926 2189 901 262 1553 Fragments arranged by size: 2189 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3819 493 Size: 3326 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6028 SphI G_CATG'C Cuts at: 1132 3386 1132 Size: 2254 3774 SspI AAT'ATT Cuts at: 5 52 3952 5 Size: 47 3900 2081 Fragments arranged by size: 3900 2081 47 StuI AGG'CCT Cuts at: 1517 2588 1517 Size: 1071 4957 StyI C'CwwG_G Cuts at: 513 1065 2004 2532 2583 3467 513 Size: 552 939 528 51 884 3074 Fragments arranged by size: 3074 939 884 552 528 51 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2941 3098 4055 4072 4225 4410 4855 Size: 1255 1361 157 957 17 153 185 445 Cuts at: 4855 325 Size: 1498 Fragments arranged by size: 1498 1361 1255 957 445 185 157 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4274 5986 96 Size: 274 80 33 51 1443 2297 1712 138 Fragments arranged by size: 2297 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2287 2319 2991 3023 5988 6020 2287 Size: 32 672 32 2965 32 2295 Fragments arranged by size: 2965 2295 672 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2550 1316 Size: 1234 4794 XbaI T'CTAG_A Cuts at: 3364 3364 Size: 6028 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2257 3322 2257 Size: 1065 4963 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6028 XmaI C'CCGG_G Cuts at: 1870 2799 3353 1870 Size: 929 554 4545 Fragments arranged by size: 4545 929 554 XmnI GAAnn'nnTTC Cuts at: 4157 4157 Size: 6028 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3836 276 Size: 53 83 186 936 2302 2468 Fragments arranged by size: 2468 2302 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SanDI SexAI SfoI SrfI SwaI