Restriction map for pFN21AB7987
 (Linear) (Six-base) MAPSORT of: KIBB7987.seq  Check: 1981  from: 1  to: 1323



KIBB7987.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:25  ..

Acc65I G'GTAC_C

Cuts at:      0    1142    1323 
   Size:       1142     181
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     154     856     873     931    1060    1323 
   Size:        154     702      17      58     129     263

  Fragments arranged by size: 

                 702     263     154     129      58      17
 
AhdI GACnn_n'nnGTC

Cuts at:      0     168     321    1323 
   Size:        168     153    1002

  Fragments arranged by size: 

                1002     168     153
 
AleI CACnn'nnGTG

Cuts at:      0    1310    1323 
   Size:       1310      13
 
AlwNI CAG_nnn'CTG

Cuts at:      0     447     675     692     834    1189    1323 
   Size:        447     228      17     142     355     134

  Fragments arranged by size: 

                 447     355     228     142     134      17
 
ApaI G_GGCC'C

Cuts at:      0      68     219     907    1323 
   Size:         68     151     688     416

  Fragments arranged by size: 

                 688     416     151      68
 
ApaLI G'TGCA_C

Cuts at:      0     871    1108    1323 
   Size:        871     237     215

  Fragments arranged by size: 

                 871     237     215
 
AvaI C'yCGr_G

Cuts at:      0     794    1288    1323 
   Size:        794     494      35

  Fragments arranged by size: 

                 794     494      35
 
BamHI G'GATC_C

Cuts at:      0     512     848    1025    1323 
   Size:        512     336     177     298

  Fragments arranged by size: 

                 512     336     298     177
 
BanI G'GyrC_C

Cuts at:      0    1142    1323 
   Size:       1142     181
 
BanII G_rGCy'C

Cuts at:      0      68     219     907    1121    1323 
   Size:         68     151     688     214     202

  Fragments arranged by size: 

                 688     214     202     151      68
 
BglI GCCn_nnn'nGGC

Cuts at:      0     151    1323 
   Size:        151    1172
 
Bme1580I G_kGCm'C

Cuts at:      0      68     219     362     460     875     907    1112    1323 
   Size:         68     151     143      98     415      32     205     211

  Fragments arranged by size: 

                 415     211     205     151     143      98      68      32
            
 
BmgBI CAC'GTC

Cuts at:      0     141    1323 
   Size:        141    1182
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1092    1323 
   Size:       1092     231
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     277    1323 
   Size:        277    1046
 
Bpu10I CC'TnA_GC

Cuts at:      0    1115    1323 
   Size:       1115     208
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     517    1295    1323 
   Size:        517     778      28

  Fragments arranged by size: 

                 778     517      28
 
BsaBI GATnn'nnATC

Cuts at:      0     127    1323 
   Size:        127    1196
 
BsaHI Gr'CG_yC

Cuts at:      0      41     367     496    1323 
   Size:         41     326     129     827

  Fragments arranged by size: 

                 827     326     129      41
 
BsaWI w'CCGG_w

Cuts at:      0     287    1323 
   Size:        287    1036
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     391    1000    1323 
   Size:        391     609     323

  Fragments arranged by size: 

                 609     391     323
 
BseYI C'CCAG_C

Cuts at:      0     642    1182    1207    1323 
   Size:        642     540      25     116

  Fragments arranged by size: 

                 642     540     116      25
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      55      96     421     688     918    1323 
   Size:         55      41     325     267     230     405

  Fragments arranged by size: 

                 405     325     267     230      55      41
 
BsiEI CG_ry'CG

Cuts at:      0     329     386    1323 
   Size:        329      57     937

  Fragments arranged by size: 

                 937     329      57
 
BsiHKAI G_wGCw'C

Cuts at:      0     602     725     875    1112    1121    1323 
   Size:        602     123     150     237       9     202

  Fragments arranged by size: 

                 602     237     202     150     123       9
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     373    1323 
   Size:        373     950
 
Bsp1286I G_dGCh'C

Cuts at:      0      68     219     362     460     602     725     875     907 
   Size:         68     151     143      98     142     123     150      32

Cuts at:    907    1112    1121    1323 
   Size:        205       9     202

  Fragments arranged by size: 

                 205     202     151     150     143     142     123      98
                  68      32       9
 
BspEI T'CCGG_A

Cuts at:      0     287    1323 
   Size:        287    1036
 
BspHI T'CATG_A

Cuts at:      0      56    1323 
   Size:         56    1267
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     683    1130    1323 
   Size:        683     447     193

  Fragments arranged by size: 

                 683     447     193
 
BsrBI CCG'CTC

Cuts at:      0     636    1323 
   Size:        636     687
 
BsrDI GCAATG_nn'

Cuts at:      0      91     341    1323 
   Size:         91     250     982

  Fragments arranged by size: 

                 982     250      91
 
BsrFI r'CCGG_y

Cuts at:      0     419    1323 
   Size:        419     904
 
BssSI C'ACGA_G

Cuts at:      0     461     874    1323 
   Size:        461     413     449

  Fragments arranged by size: 

                 461     449     413
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     675    1323 
   Size:        675     648
 
BstYI r'GATC_y

Cuts at:      0     182     482     512     848    1001    1025    1323 
   Size:        182     300      30     336     153      24     298

  Fragments arranged by size: 

                 336     300     298     182     153      30      24
 
BtgI C'CryG_G

Cuts at:      0      76     362     516     852    1227    1323 
   Size:         76     286     154     336     375      96

  Fragments arranged by size: 

                 375     336     286     154      96      76
 
BtsI GCAGTG_nn'

Cuts at:      0     303     569     705    1323 
   Size:        303     266     136     618

  Fragments arranged by size: 

                 618     303     266     136
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     751    1102    1323 
   Size:        751     351     221

  Fragments arranged by size: 

                 751     351     221
 
EaeI y'GGCC_r

Cuts at:      0     102     143     326     383    1272    1323 
   Size:        102      41     183      57     889      51

  Fragments arranged by size: 

                 889     183     102      57      51      41
 
EagI C'GGCC_G

Cuts at:      0     326     383    1323 
   Size:        326      57     940

  Fragments arranged by size: 

                 940     326      57
 
EarI CTCTTCn'nnn_

Cuts at:      0     607     685    1323 
   Size:        607      78     638

  Fragments arranged by size: 

                 638     607      78
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1309    1323 
   Size:       1309      14
 
EcoICRI GAG'CTC

Cuts at:      0    1119    1323 
   Size:       1119     204
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     154     277     856     873     931    1060    1323 
   Size:        154     123     579      17      58     129     263

  Fragments arranged by size: 

                 579     263     154     129     123      58      17
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     202     838    1137    1323 
   Size:        202     636     299     186

  Fragments arranged by size: 

                 636     299     202     186
 
EcoO109I rG'GnC_Cy

Cuts at:      0      27      65     195     215     216     252     672     773 
   Size:         27      38     130      20       1      36     420     101

Cuts at:    773     831     903     904    1323 
   Size:         58      72       1     419

  Fragments arranged by size: 

                 420     419     130     101      72      58      38      36
                  27      20       1       1
 
HaeII r_GCGC'y

Cuts at:      0     448     506     571     643     823    1214    1323 
   Size:        448      58      65      72     180     391     109

  Fragments arranged by size: 

                 448     391     180     109      72      65      58
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      38      70     154     186     307     339     481     513 
   Size:         38      32      84      32     121      32     142      32

Cuts at:    513     952     984    1323 
   Size:        439      32     339

  Fragments arranged by size: 

                 439     339     142     121      84      38      32      32
                  32      32      32
 
HincII GTy'rAC

Cuts at:      0    1053    1323 
   Size:       1053     270
 
KpnI G_GTAC'C

Cuts at:      0    1146    1323 
   Size:       1146     177
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      69     602     860    1323 
   Size:         69     533     258     463

  Fragments arranged by size: 

                 533     463     258      69
 
MscI TGG'CCA

Cuts at:      0     145    1274    1323 
   Size:        145    1129      49

  Fragments arranged by size: 

                1129     145      49
 
MslI CAynn'nnrTG

Cuts at:      0     976    1310    1323 
   Size:        976     334      13

  Fragments arranged by size: 

                 976     334      13
 
MspA1I CmG'CkG

Cuts at:      0      78     364     518     854    1186    1323 
   Size:         78     286     154     336     332     137

  Fragments arranged by size: 

                 336     332     286     154     137      78
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1082    1323 
   Size:       1082     241
 
PpuMI rG'GwC_Cy

Cuts at:      0     195     252     672    1323 
   Size:        195      57     420     651

  Fragments arranged by size: 

                 651     420     195      57
 
PspOMI G'GGCC_C

Cuts at:      0      64     215     903    1323 
   Size:         64     151     688     420

  Fragments arranged by size: 

                 688     420     151      64
 
PstI C_TGCA'G

Cuts at:      0     653     671    1323 
   Size:        653      18     652

  Fragments arranged by size: 

                 653     652      18
 
PvuII CAG'CTG

Cuts at:      0    1186    1323 
   Size:       1186     137
 
SacI G_AGCT'C

Cuts at:      0    1121    1323 
   Size:       1121     202
 
SacII CC_GC'GG

Cuts at:      0      79     365     519     855    1323 
   Size:         79     286     154     336     468

  Fragments arranged by size: 

                 468     336     286     154      79
 
SapI GCTCTTCn'nnn_

Cuts at:      0     607     685    1323 
   Size:        607      78     638

  Fragments arranged by size: 

                 638     607      78
 
SbfI CC_TGCA'GG

Cuts at:      0     671    1323 
   Size:        671     652
 
SfcI C'TryA_G

Cuts at:      0     649     667    1134    1323 
   Size:        649      18     467     189

  Fragments arranged by size: 

                 649     467     189      18
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     151    1323 
   Size:        151    1172
 
SmaI CCC'GGG

Cuts at:      0     796    1323 
   Size:        796     527
 
StuI AGG'CCT

Cuts at:      0     583    1323 
   Size:        583     740
 
StyI C'CwwG_G

Cuts at:      0     527     578    1323 
   Size:        527      51     745

  Fragments arranged by size: 

                 745     527      51
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     936    1093    1323 
   Size:        936     157     230

  Fragments arranged by size: 

                 936     230     157
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     282     314     986    1018    1323 
   Size:        282      32     672      32     305

  Fragments arranged by size: 

                 672     305     282      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     545    1323 
   Size:        545     778
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     252    1323 
   Size:        252    1071
 
XmaI C'CCGG_G

Cuts at:      0     794    1323 
   Size:        794     529
 

 Enzymes that do cut:

   Acc65I     AcuI     AhdI     AleI    AlwNI     ApaI    ApaLI     AvaI
    BamHI     BanI    BanII     BglI Bme1580I    BmgBI     BmrI     BpmI
   Bpu10I     BsaI    BsaBI    BsaHI    BsaWI    BseRI    BseYI     BsgI
    BsiEI  BsiHKAI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrFI    BssSI   BstAPI    BstYI     BtgI     BtsI     DrdI
     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I
    HaeII    Hin4I   HincII     KpnI     MmeI     MscI     MslI   MspA1I
    PflMI    PpuMI   PspOMI     PstI    PvuII     SacI    SacII     SapI
     SbfI     SfcI     SfiI     SmaI     StuI     StyI    TaqII     TstI
  Tth111I     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AfeI    AflII   AflIII     AgeI
     AlfI     AloI     ApoI     AscI     AseI    AsiSI    AvrII     BaeI
     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI    BglII
     BlpI     BmtI     BplI    BpuEI    BsaAI    BsaXI    BsiWI     BsmI
    BsrGI   BssHII    BstBI   BstEII    BstXI  BstZ17I   Bsu36I    BtgZI
     ClaI    CspCI     DraI   DraIII    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI  HindIII     HpaI     KasI     MfeI     MluI     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI     PfoI     PmeI     PmlI     PpiI
    PshAI     PsiI    PspXI     PsrI     PvuI    RsrII     SalI    SanDI
     ScaI    SexAI     SfoI     SgfI    SgrAI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     SwaI     TatI     XbaI     XhoI     XmnI
     ZraI
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