Restriction map for
pFN21AB7973
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# NO CUT ENZYMES ( 142 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AfaI AflII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AviII AvrII BamHI
BanII BbrPI BbvCI BciVI BclI
BfaI BfrI BglI BglII BlnI
BpmI BsaAI BsaBI BsaWI BseAI
BsiEI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspLU11I BspMI BsrBI
BsrDI BsrGI BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstYI BstZ17I
Bsu36I ClaI CpoI DraI DraIII
DsaI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HincII HindII HindIII HpaI KpnI
KspI MaeI MamI MflI MluI
MroI MseI NaeI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflMI
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PstI PvuI RsaI RsrII
SacI SacII SalI SapI SbfI
ScaI SfiI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI Tru9I Van91I
XbaI XcmI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 64 enzymes) #
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AccII 142 CGCG CG^CG
AflIII 467 ACGTGT A^C[GA][CT]GT
AlwI 563 GGATC GGATC(4/5)
AspHI 571 GAGCAC G[AT]GC[AT]^C
AvaI 22 CCCGAG C^[CT]CG[GA]G
BalI 524 TGGCCA TGG^CCA
BanI 13 GGCGCC G^G[CT][GA]CC
BbeI 13 GGCGCC GGCGC^C
BbsI 553 GTCTTC GAAGAC(2/6)
BbvI 228 GCAGC GCAGC(8/12)
BcgI 263 GCAAAGCCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 10 CCGGG CC^[GC]GG
BmyI 571 GAGCAC G[AGT]GC[ACT]^C
BpuAI 553 GTCTTC GAAGAC(2/6)
BsaI 444 GGTCTC GGTCTC(1/5)
BsgI 616 CTGCAC GTGCAG(16/14)
BsiHKAI 571 GAGCAC G[AT]GC[AT]^C
BsmAI 445 GTCTC GTCTC(1/5)
BsoBI 22 CCCGAG C^[CT]CG[GA]G
Bsp1286I 571 GAGCAC G[AGT]GC[ACT]^C
BspHI 377 TCATGA T^CATGA
BsrFI 131 ACCGGC [GA]^CCGG[CT]
BsrI 498 CCAGT ACTGG(1/-1)
BssHII 141 GCGCGC G^CGCGC
BstF5I 357 GGATG GGATG(2/0)
BstUI 142 CGCG CG^CG
BstXI 515 CCATCTCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 131 ACCGGC [GA]^CCGG[CT]
DraII 26 AGGACCC [GA]G^G[ACGT]CC[CT]
DrdI 28 GACCCTTGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 524 TGGCCA [CT]^GGCC[GA]
EarI 165 GAAGAG CTCTTC(1/4)
EcoO109I 26 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoT14I 31 CCTTGG C^C[AT][AT]GG
FauI 594 GCGGG CCCGC(4/6)
FokI 357 GGATG GGATG(9/13)
HaeII 13 GGCGCC [GA]GCGC^[CT]
HphI 362 TCACC GGTGA(8/7)
KasI 13 GGCGCC G^GCGCC
Ksp632I 165 GAAGAG CTCTTC(1/4)
MfeI 622 CAATTG C^AATTG
MluNI 524 TGGCCA TGG^CCA
MscI 524 TGGCCA TGG^CCA
MspA1I 558 CAGCTG C[AC]G^C[GT]G
MunI 622 CAATTG C^AATTG
MvnI 142 CGCG CG^CG
NarI 13 GGCGCC GG^CGCC
NciI 10 CCGGG CC^[GC]GG
PleI 118 GAGTC GAGTC(4/5)
PpuMI 26 AGGACCC [GA]G^G[AT]CC[CT]
PshAI 207 GACCATCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 558 CAGCTG CAG^CTG
RcaI 377 TCATGA T^CATGA
SfaNI 479 GCATC GCATC(5/9)
SfcI 54 CTACAG C^T[GA][CT]AG
SfoI 13 GGCGCC GGC^GCC
StuI 107 AGGCCT AGG^CCT
StyI 31 CCTTGG C^C[AT][AT]GG
TaqI 566 TCGA T^CGA
TfiI 487 GATTC G^A[AT]TC
TseI 228 GCAGC G^C[AT]GC
Tsp45I 361 GTCAC ^GT[GC]AC
Tsp509I 623 AATT ^AATT
TthHB8I 566 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AlwNI 535 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
586 CAGACCCTG
AspI 439 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
451 GACCAGGTC
BlpI 157 GCTGAGC GC^T[ACGT]AGC
258 GCTCAGC
Bpu1102I 157 GCTGAGC GC^T[ACGT]AGC
258 GCTCAGC
BsaJI 23 CCGAGG C^C[ACGT][ACGT]GG
31 CCTTGG
BsmFI 173 GGGAC GGGAC(10/14)
214 GTCCC
CelII 157 GCTGAGC GC^T[ACGT]AGC
258 GCTCAGC
DpnII 375 GATC ^GATC
564 GATC
HaeIII 108 GGCC GG^CC
525 GGCC
HapII 10 CCGG C^CGG
132 CCGG
HgaI 175 GACGC GACGC(5/10)
611 GCGTC
HinfI 118 GAGTC G^A[ACGT]TC
487 GATTC
HpaII 10 CCGG C^CGG
132 CCGG
MaeII 346 ACGT A^CGT
467 ACGT
MaeIII 348 GTTAC ^GT[ACGT]AC
361 GTCAC
MboI 375 GATC ^GATC
564 GATC
MslI 378 CATGAAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
516 CATCTCAGTG
MspI 10 CCGG C^CGG
132 CCGG
MwoI 59 GCGACAACAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
573 GCACTGTAAGC
NdeII 375 GATC ^GATC
564 GATC
PflFI 439 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
451 GACCAGGTC
Sau3AI 375 GATC ^GATC
564 GATC
SexAI 440 ACCAGGT A^CC[AT]GGT
452 ACCAGGT
Tth111I 439 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
451 GACCAGGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 28 enzymes) #
#########################################
AciI AcyI AluI AvaII BsaHI
BseRI BsiYI BslI BssKI BstNI
Cac8I CfoI Cfr13I DdeI EcoRII
Fnu4HI HhaI Hin1I HinP1I ItaI
MboII MnlI MvaI NlaIII NlaIV
Sau96I ScrFI TspRI