Restriction map for
pFN21AB7958
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# NO CUT ENZYMES ( 154 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AgeI Alw44I AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp718I AspHI AspI AvaI
AviII AvrII BalI BanI BanII
BbeI BbrPI BbsI BbvCI BcgI
BcnI BglI BglII BlnI BlpI
BmyI Bpu1102I BpuAI BsaAI BsaBI
BsaHI BsaWI BseAI BsgI BsiEI
BsiHKAI BsiWI BsmBI BsmI BsoBI
Bsp1286I BspDI BspEI BspMI BsrBI
BsrFI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstUI BstZ17I Bsu36I
CelII CfoI Cfr10I ClaI CpoI
DraI DraIII DrdI EaeI EagI
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FauI
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I HindIII HpaI KasI
KpnI KspI MamI MfeI MluI
MluNI MroI MscI MspA1I MunI
MvnI NaeI NarI NciI NcoI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI PvuII RsrII
SacI SacII SalI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
StuI StyI SwaI Tth111I Van91I
XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AflII 988 CTTAAG C^TTAAG
AhdI 514 GACTTGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I 1099 GAACCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 514 GACTTGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 526 GGATCC G^GATCC
BclI 678 TGATCA T^GATCA
BfaI 488 CTAG C^TAG
BfrI 988 CTTAAG C^TTAAG
BsaI 581 GGTCTC GGTCTC(1/5)
BsaJI 123 CCACGG C^C[ACGT][ACGT]GG
BseRI 778 CTCCTC GAGGAG(10/8)
BsiYI 157 CCTGTTGAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 157 CCTGTTGAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
Bsp1407I 312 TGTACA T^GTACA
BspHI 327 TCATGA T^CATGA
BsrDI 945 CATTGC GCAATG(2/0)
BsrGI 312 TGTACA T^GTACA
BstXI 123 CCACGGCGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 526 GGATCC [GA]^GATC[CT]
Cac8I 19 GCACGC GC[ACGT]^[ACGT]GC
DdeI 966 CTCAG C^T[ACGT]AG
DsaI 123 CCACGG C^C[GA][CT]GG
Eam1105I 514 GACTTGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 157 CCTGTTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 678 TGATCA T^GATCA
HaeIII 1149 GGCC GG^CC
HapII 1147 CCGG C^CGG
HpaII 1147 CCGG C^CGG
HphI 774 TCACC GGTGA(8/7)
MaeI 488 CTAG C^TAG
MaeII 588 ACGT A^CGT
MflI 526 GGATCC [GA]^GATC[CT]
MspI 1147 CCGG C^CGG
PleI 64 GAGTC GAGTC(4/5)
RcaI 327 TCATGA T^CATGA
SapI 650 GAAGAGC GCTCTTC(1/4)
SspBI 312 TGTACA T^GTACA
SspI 564 AATATT AAT^ATT
TaqI 801 TCGA T^CGA
TthHB8I 801 TCGA T^CGA
XcmI 461 CCAAGCACTGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 526 GGATCC [GA]^GATC[CT]
XmnI 1099 GAACCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
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AciI 17 CCGC CCGC(-3/-1)
128 GCGG
AflIII 173 ACATGT A^C[GA][CT]GT
247 ACATGT
AluI 352 AGCT AG^CT
705 AGCT
AlwI 527 GATCC GGATC(4/5)
526 GGATC
AlwNI 1059 CAGAGACTG CAG[ACGT][ACGT][ACGT]^CTG
1103 CAGTTCCTG
AvaII 39 GGACC G^G[AT]CC
493 GGACC
BbvI 1169 GCAGC GCAGC(8/12)
905 GCTGC
BciVI 381 GGATAC GTATCC(6/5)
321 GTATCC
BsmFI 84 GGGAC GGGAC(10/14)
993 GGGAC
BspLU11I 173 ACATGT A^CATGT
247 ACATGT
BstAPI 718 GCAAACACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1058 GCAGAGACTGC
Cfr13I 39 GGACC G^G[ACGT]CC
493 GGACC
DraII 38 AGGACCC [GA]G^G[ACGT]CC[CT]
492 AGGACCC
EarI 44 CTCTTC CTCTTC(1/4)
650 GAAGAG
EcoO109I 38 AGGACCC [GA]G^G[ACGT]CC[CT]
492 AGGACCC
Fnu4HI 905 GCTGC GC^[ACGT]GC
1169 GCAGC
HincII 66 GTCAAC GT[CT]^[GA]AC
1009 GTTGAC
HindII 66 GTCAAC GT[CT]^[GA]AC
1009 GTTGAC
ItaI 905 GCTGC GC^[ACGT]GC
1169 GCAGC
Ksp632I 44 CTCTTC CTCTTC(1/4)
650 GAAGAG
MwoI 718 GCAAACACTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
1058 GCAGAGACTGC
NdeI 442 CATATG CA^TATG
932 CATATG
PpuMI 38 AGGACCC [GA]G^G[AT]CC[CT]
492 AGGACCC
PstI 401 CTGCAG CTGCA^G
906 CTGCAG
Sau96I 39 GGACC G^G[ACGT]CC
493 GGACC
SfaNI 1159 GATGC GCATC(5/9)
655 GCATC
SmlI 217 CTTGAG C^T[CT][GA]AG
988 CTTAAG
TfiI 119 GAATC G^A[AT]TC
712 GAATC
TseI 905 GCTGC G^C[AT]GC
1169 GCAGC
#########################################
# 3 OR MORE CUT ENZYMES ( 32 enzymes) #
#########################################
AcsI AfaI ApoI BpmI BsmAI
BsrI BssKI BstF5I BstNI DpnII
EcoRII FokI HinfI MaeIII MboI
MboII MnlI MseI MslI MvaI
NdeII NlaIII NlaIV NspI RsaI
Sau3AI ScrFI SfcI Tru9I Tsp45I
Tsp509I TspRI