Restriction map for
pFN21AB7956
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# NO CUT ENZYMES ( 143 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AcyI AflII AgeI AhdI Alw44I
AocI Aor51HI ApaLI ApoI AscI
AseI AsnI Asp718I AspEI AspHI
AspI AvaI AviII AvrII BalI
BbeI BbrPI BbsI BbvCI BcgI
BciVI BclI BcnI BfrI BglII
BlnI BpuAI BsaAI BsaBI BsaHI
BseAI BsiEI BsiHKAI BsiWI BsmBI
BsmI BsoBI BspDI BspEI BspHI
BspLU11I BsrBI BsrDI BsrFI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstXI BstZ17I Bsu36I Cfr10I
ClaI DraI DrdI EaeI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoO65I EcoRI EcoT22I FauI
FbaI FseI FspI HaeII Hin1I
HpaI KasI KpnI Ksp632I KspI
MamI MfeI MluNI MroI MscI
MseI MunI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
SacI SacII SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I SspI
SwaI Tru9I Tth111I XbaI XcmI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AccI 953 GTCGAC GT^[AC][GT]AC
AccII 30 CGCG CG^CG
AflIII 29 ACGCGT A^C[GA][CT]GT
ApaI 359 GGGCCC GGGCC^C
Asp700I 207 GAAGCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI 67 GGATCC G^GATCC
BglI 307 GCCCCCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 823 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 823 GCTCAGC GC^T[ACGT]AGC
BsaI 1042 GAGACC GGTCTC(1/5)
BsgI 758 CTGCAC GTGCAG(16/14)
BsmAI 1042 GAGAC GTCTC(1/5)
Bsp1407I 532 TGTACA T^GTACA
BsrGI 532 TGTACA T^GTACA
BstUI 30 CGCG CG^CG
CelII 823 GCTCAGC GC^T[ACGT]AGC
CfoI 1152 GCGC GCG^C
CpoI 1159 CGGTCCG CG^G[AT]CCG
DraIII 988 CACGGAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 165 CCGTGG C^C[GA][CT]GG
EcoRV 52 GATATC GAT^ATC
HhaI 1152 GCGC GCG^C
HinP1I 1152 GCGC G^CGC
HincII 953 GTCGAC GT[CT]^[GA]AC
HindII 953 GTCGAC GT[CT]^[GA]AC
HindIII 208 AAGCTT A^AGCTT
MaeII 1025 ACGT A^CGT
MluI 29 ACGCGT A^CGCGT
MslI 1171 CATGCAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 835 CAGCTG C[AC]G^C[GT]G
MvnI 30 CGCG CG^CG
NspI 1170 GCATGC [GA]CATG^[CT]
PpuMI 647 GGGTCCT [GA]G^G[AT]CC[CT]
PstI 838 CTGCAG CTGCA^G
PvuII 835 CAGCTG CAG^CTG
RsrII 1159 CGGTCCG CG^G[AT]CCG
SalI 953 GTCGAC G^TCGAC
SphI 1170 GCATGC GCATG^C
SspBI 532 TGTACA T^GTACA
StuI 399 AGGCCT AGG^CCT
XmnI 207 GAAGCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
BanI 444 GGTGCC G^G[CT][GA]CC
511 GGCACC
BfaI 119 CTAG C^TAG
418 CTAG
BseRI 574 GAGGAG GAGGAG(10/8)
640 GAGGAG
BspMI 20 ACCTGC ACCTGC(4/8)
620 ACCTGC
BstYI 3 GGATCT [GA]^GATC[CT]
67 GGATCC
EcoNI 627 CCTCACCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
651 CCTCAAGAAGG
HphI 618 TCACC GGTGA(8/7)
629 TCACC
MaeI 119 CTAG C^TAG
418 CTAG
MflI 3 GGATCT [GA]^GATC[CT]
67 GGATCC
PflMI 789 CCAGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
958 CCAGAAGCTGG
SfcI 124 CTACAG C^T[GA][CT]AG
838 CTGCAG
SmlI 652 CTCAAG C^T[CT][GA]AG
1117 CTTGAG
TaqI 61 TCGA T^CGA
954 TCGA
Tsp45I 260 GTGAC ^GT[GC]AC
994 GTGAC
Tsp509I 773 AATT ^AATT
849 AATT
TthHB8I 61 TCGA T^CGA
954 TCGA
Van91I 789 CCAGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
958 CCAGAAGCTGG
XhoII 3 GGATCT [GA]^GATC[CT]
67 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 56 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AvaII BanII BbvI BmyI BpmI
BsaJI BsaWI BsiYI BslI BsmFI
Bsp1286I BsrI BssKI BstF5I BstNI
Cac8I Cfr13I DdeI DpnII DraII
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HgaI HinfI HpaII
ItaI MaeIII MboI MboII MnlI
MspI MvaI MwoI NdeII NlaIII
NlaIV PleI RsaI Sau3AI Sau96I
ScrFI SfaNI StyI TfiI TseI
TspRI