(Circular) (Six-base) MAPSORT of: pFN21AB7956.seq Check: 5401 from: 1 to: 5890 pFN21AB7956 5890 bp With 182 enzymes: SgfI * April 15, 2010 09:28 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2634 2634 Size: 5890 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3700 278 Size: 53 83 186 936 2164 2468 Fragments arranged by size: 2468 2164 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2959 3233 1937 Size: 1022 274 4594 Fragments arranged by size: 4594 1022 274 Acc65I G'GTAC_C Cuts at: 3211 3211 Size: 5890 AclI AA'CG_TT Cuts at: 1563 4017 4390 1563 Size: 2454 373 3063 Fragments arranged by size: 3063 2454 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2220 2926 3953 5337 5605 1632 Size: 588 706 1027 1384 268 1917 Fragments arranged by size: 1917 1384 1027 706 588 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5693 AflIII A'CryG_T Cuts at: 1203 2034 4789 5766 1203 Size: 831 2755 977 1327 Fragments arranged by size: 2755 1327 977 831 AgeI A'CCGG_T Cuts at: 4721 4721 Size: 5890 AhdI GACnn_n'nnGTC Cuts at: 4619 4619 Size: 5890 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5890 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5890 AlwNI CAG_nnn'CTG Cuts at: 2143 2800 2969 3633 5205 2143 Size: 657 169 664 1572 2828 Fragments arranged by size: 2828 1572 664 657 169 ApaI G_GGCC'C Cuts at: 2368 2368 Size: 5890 ApaLI G'TGCA_C Cuts at: 3947 5103 3947 Size: 1156 4734 ApoI r'AATT_y Cuts at: 1493 3199 3425 3577 5686 1493 Size: 1706 226 152 2109 1697 Fragments arranged by size: 2109 1706 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4444 160 Size: 872 3412 1606 Fragments arranged by size: 3412 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5890 AvaI C'yCGr_G Cuts at: 1870 1943 3215 1870 Size: 73 1272 4545 Fragments arranged by size: 4545 1272 73 BamHI G'GATC_C Cuts at: 2072 3220 3587 2072 Size: 1148 367 4375 Fragments arranged by size: 4375 1148 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2449 2516 3211 4666 618 Size: 325 217 627 662 67 695 1455 1842 Fragments arranged by size: 1842 1455 695 662 627 325 217 67 BanII G_rGCy'C Cuts at: 1391 2049 2368 2438 3209 1391 Size: 658 319 70 771 4072 Fragments arranged by size: 4072 771 658 319 70 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4919 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4081 4115 1903 Size: 34 2144 34 3678 Fragments arranged by size: 3678 2144 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3782 4992 3782 Size: 1210 4680 BclI T'GATC_A Cuts at: 1548 1548 Size: 5890 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2318 4501 136 Size: 107 122 71 631 605 646 2183 1525 Fragments arranged by size: 2183 1525 646 631 605 122 107 71 BglII A'GATC_T Cuts at: 5885 5885 Size: 5890 BlpI GC'TnA_GC Cuts at: 2829 3307 2829 Size: 478 5412 Bme1580I G_kGCm'C Cuts at: 1790 2368 2535 3951 5107 1790 Size: 578 167 1416 1156 2573 Fragments arranged by size: 2573 1416 1156 578 167 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5890 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2400 2522 3144 Size: 416 53 83 399 24 983 122 622 Cuts at: 3144 3621 4579 442 Size: 477 958 1753 Fragments arranged by size: 1753 983 958 622 477 416 399 122 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5890 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2063 2091 2102 2390 2759 2822 3065 3105 Size: 824 28 11 288 369 63 243 40 Cuts at: 3105 4550 1239 Size: 1445 2579 Fragments arranged by size: 2579 1445 824 369 288 243 63 40 28 11 Bpu10I CC'TnA_GC Cuts at: 2697 2697 Size: 5890 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2642 3143 4015 4880 5178 2642 Size: 501 872 865 298 3354 Fragments arranged by size: 3354 872 865 501 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3041 4553 882 Size: 617 1542 1512 2219 Fragments arranged by size: 2219 1542 1512 617 BsaAI yAC'GTr Cuts at: 493 1204 3681 493 Size: 711 2477 2702 Fragments arranged by size: 2702 2477 711 BsaBI GATnn'nnATC Cuts at: 5884 5884 Size: 5890 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3697 4079 4714 275 Size: 53 83 186 936 2164 382 635 1451 Fragments arranged by size: 2164 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2116 2159 3162 4323 4721 4995 5142 5553 2116 Size: 43 1003 1161 398 274 147 411 2453 Fragments arranged by size: 2453 1161 1003 411 398 274 147 43 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2314 2344 2801 2831 1178 Size: 30 165 30 911 30 457 30 4237 Fragments arranged by size: 4237 911 457 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2594 2660 1186 Size: 1408 66 4416 Fragments arranged by size: 4416 1408 66 BseYI C'CCAG_C Cuts at: 2086 2437 2593 2936 2969 5093 5589 2086 Size: 351 156 343 33 2124 496 2387 Fragments arranged by size: 2387 2124 496 351 343 156 33 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2748 1163 Size: 573 1012 4305 Fragments arranged by size: 4305 1012 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3340 4101 5129 1540 Size: 275 186 1339 761 1028 2301 Fragments arranged by size: 2301 1339 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3951 4036 5107 3951 Size: 85 1071 4734 Fragments arranged by size: 4734 1071 85 BsmI GAATG_Cn' Cuts at: 3401 3494 3401 Size: 93 5797 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2049 2368 2438 2535 3209 3951 4036 Size: 399 259 319 70 97 674 742 85 Cuts at: 4036 5107 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 674 399 319 259 97 85 70 BspEI T'CCGG_A Cuts at: 5553 5553 Size: 5890 BspHI T'CATG_A Cuts at: 3779 5509 3779 Size: 1730 4160 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2034 2634 3245 844 Size: 1190 600 611 3489 Fragments arranged by size: 3489 1190 611 600 BsrBI CCG'CTC Cuts at: 1394 1823 3777 1394 Size: 429 1954 3507 Fragments arranged by size: 3507 1954 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4385 4559 65 Size: 1183 3137 174 1396 Fragments arranged by size: 3137 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4534 4721 1700 Size: 110 2724 187 2869 Fragments arranged by size: 2869 2724 187 110 BsrGI T'GTAC_A Cuts at: 96 2537 96 Size: 2441 3449 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5890 BssSI C'ACGA_G Cuts at: 3950 4962 3950 Size: 1012 4878 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5890 BstBI TT'CG_AA Cuts at: 4694 4694 Size: 5890 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5890 BstYI r'GATC_y Cuts at: 1972 2008 2072 3220 3587 3972 3989 5441 5527 Size: 36 64 1148 367 385 17 1452 86 Cuts at: 5527 5539 5885 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1148 385 367 346 86 64 36 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2170 513 Size: 552 939 166 4233 Fragments arranged by size: 4233 939 552 166 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5151 Fragments arranged by size: 5151 683 56 BtsI GCAGTG_nn' Cuts at: 2095 2269 3116 3406 4200 4220 2095 Size: 174 847 290 794 20 3765 Fragments arranged by size: 3765 847 794 290 174 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5890 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5855 DraI TTT'AAA Cuts at: 3194 3194 Size: 5890 DraIII CAC_nnn'GTG Cuts at: 2998 2998 Size: 5890 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4897 809 Size: 507 3581 1802 Fragments arranged by size: 3581 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3337 4226 5855 Size: 54 1425 50 275 18 1507 889 1629 Cuts at: 5855 8 Size: 43 Fragments arranged by size: 1629 1507 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3337 1537 Size: 275 1525 4090 Fragments arranged by size: 4090 1525 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3820 1353 Size: 2467 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2728 2825 4477 4851 4997 387 Size: 1064 138 1139 97 1652 374 146 1280 Fragments arranged by size: 1652 1280 1139 1064 374 146 138 97 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2063 2091 2102 2220 2390 2759 2822 Size: 393 431 28 11 118 170 369 63 Cuts at: 2822 2926 3065 3105 3953 4550 5337 5605 1239 Size: 104 139 40 848 597 787 268 1524 Fragments arranged by size: 1524 848 787 597 431 393 369 268 170 139 118 104 63 40 28 11 EcoNI CCTnn'n_nnAGG Cuts at: 2636 2660 2636 Size: 24 5866 EcoO109I rG'GnC_Cy Cuts at: 2364 2365 2653 2364 Size: 1 288 5601 Fragments arranged by size: 5601 288 1 EcoRI G'AATT_C Cuts at: 3199 3577 3199 Size: 378 5512 EcoRV GAT'ATC Cuts at: 2059 2059 Size: 5890 FspI TGC'GCA Cuts at: 4396 4396 Size: 5890 HaeII r_GCGC'y Cuts at: 1678 5037 1678 Size: 3359 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2048 2080 4531 4563 4605 Size: 32 135 32 476 32 2451 32 42 Cuts at: 4605 4637 1373 Size: 32 2626 Fragments arranged by size: 2626 2451 476 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2960 3234 3480 669 Size: 988 281 1022 274 246 3079 Fragments arranged by size: 3079 1022 988 281 274 246 HindIII A'AGCT_T Cuts at: 748 2213 748 Size: 1465 4425 HpaI GTT'AAC Cuts at: 3480 3480 Size: 5890 KpnI G_GTAC'C Cuts at: 3215 3215 Size: 5890 MfeI C'AATT_G Cuts at: 3489 3489 Size: 5890 MluI A'CGCG_T Cuts at: 2034 5766 2034 Size: 3732 2158 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2892 5004 5188 1305 Size: 1587 2112 184 2007 Fragments arranged by size: 2112 2007 1587 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4068 Fragments arranged by size: 4068 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3180 3848 4207 4366 518 Size: 648 2014 668 359 159 2042 Fragments arranged by size: 2042 2014 668 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2842 3306 3983 5131 5376 1606 Size: 1236 464 677 1148 245 2120 Fragments arranged by size: 2120 1236 1148 677 464 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4399 Fragments arranged by size: 4399 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5890 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5890 NotI GC'GGCC_GC Cuts at: 3337 3337 Size: 5890 NspI r_CATG'y Cuts at: 1132 1229 3179 3248 4793 1132 Size: 97 1950 69 1545 2229 Fragments arranged by size: 2229 1950 1545 97 69 PasI CC'CwG_GG Cuts at: 1348 2316 1348 Size: 968 4922 PciI A'CATG_T Cuts at: 4789 4789 Size: 5890 PflMI CCAn_nnn'nTGG Cuts at: 1108 2800 2969 1108 Size: 1692 169 4029 Fragments arranged by size: 4029 1692 169 PfoI T'CCnGG_A Cuts at: 2079 2793 2079 Size: 714 5176 PmeI GTTT'AAAC Cuts at: 3194 3194 Size: 5890 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3959 3991 5472 5504 3959 Size: 32 1481 32 4345 Fragments arranged by size: 4345 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2653 2653 Size: 5890 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5890 PsiI TTA'TAA Cuts at: 3460 3460 Size: 5890 PspOMI G'GGCC_C Cuts at: 2364 2364 Size: 5890 PstI C_TGCA'G Cuts at: 830 2847 3242 830 Size: 2017 395 3478 Fragments arranged by size: 3478 2017 395 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5890 PvuII CAG'CTG Cuts at: 2842 2842 Size: 5890 RsrII CG'GwC_CG Cuts at: 3165 3165 Size: 5890 SalI G'TCGA_C Cuts at: 1936 2958 3232 1936 Size: 1022 274 4594 Fragments arranged by size: 4594 1022 274 SbfI CC_TGCA'GG Cuts at: 3242 3242 Size: 5890 ScaI AGT'ACT Cuts at: 4138 5850 4138 Size: 1712 4178 SfcI C'TryA_G Cuts at: 826 1045 2129 2843 3238 4373 5054 5245 826 Size: 219 1084 714 395 1135 681 191 1471 Fragments arranged by size: 1471 1135 1084 714 681 395 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5890 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5890 SmaI CCC'GGG Cuts at: 1872 3217 1872 Size: 1345 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2657 3122 3994 4895 5157 820 Size: 197 926 714 465 872 901 262 1553 Fragments arranged by size: 1553 926 901 872 714 465 262 197 SnaBI TAC'GTA Cuts at: 493 3681 493 Size: 3188 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5890 SphI G_CATG'C Cuts at: 1132 3179 3248 1132 Size: 2047 69 3774 Fragments arranged by size: 3774 2047 69 SspI AAT'ATT Cuts at: 5 52 3814 5 Size: 47 3762 2081 Fragments arranged by size: 3762 2081 47 StuI AGG'CCT Cuts at: 1517 2406 1517 Size: 889 5001 StyI C'CwwG_G Cuts at: 513 1065 2004 2637 2820 3090 3329 513 Size: 552 939 633 183 270 239 3074 Fragments arranged by size: 3074 939 633 552 270 239 183 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2588 3917 3934 4087 4272 4717 325 Size: 1255 1008 1329 17 153 185 445 1498 Fragments arranged by size: 1498 1329 1255 1008 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2238 2537 2724 Size: 274 80 33 51 1443 261 299 187 Cuts at: 2724 4136 5848 96 Size: 1412 1712 138 Fragments arranged by size: 1712 1443 1412 299 274 261 187 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2146 2178 2801 2833 5850 5882 2146 Size: 32 623 32 3017 32 2154 Fragments arranged by size: 3017 2154 623 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5890 XbaI T'CTAG_A Cuts at: 3226 3226 Size: 5890 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5890 XmaI C'CCGG_G Cuts at: 1870 3215 1870 Size: 1345 4545 XmnI GAAnn'nnTTC Cuts at: 2216 4019 2216 Size: 1803 4087 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3698 276 Size: 53 83 186 936 2164 2468 Fragments arranged by size: 2468 2164 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII RsrII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI