Restriction map for pFN21AB7927
 (Circular) (Six-base) MAPSORT of: pFN21AB7927.seq  Check: 6404  from: 1  to: 5305



pFN21AB7927  5305 bp

 With 182 enzymes: SgfI * 

                        April 15, 2010 09:28  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    3115     278 
   Size:         53      83     186     936    1579    2468

  Fragments arranged by size: 

                2468    1579     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2648    1937 
   Size:        711    4594
 
Acc65I G'GTAC_C

Cuts at:   2626    2626 
   Size:       5305
 
AclI AA'CG_TT

Cuts at:   1563    2604    3432    3805    1563 
   Size:       1041     828     373    3063

  Fragments arranged by size: 

                3063    1041     828     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2075    3368    4752    5020    1632 
   Size:        443    1293    1384     268    1917

  Fragments arranged by size: 

                1917    1384    1293     443     268
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    5108
 
AflIII A'CryG_T

Cuts at:   1203    2452    4204    5181    1203 
   Size:       1249    1752     977    1327

  Fragments arranged by size: 

                1752    1327    1249     977
 
AgeI A'CCGG_T

Cuts at:   4136    4136 
   Size:       5305
 
AhdI GACnn_n'nnGTC

Cuts at:   4034    4034 
   Size:       5305
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       5305
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       5305
 
AlwNI CAG_nnn'CTG

Cuts at:   3048    4620    3048 
   Size:       1572    3733
 
ApaLI G'TGCA_C

Cuts at:   3362    4518    3362 
   Size:       1156    4149
 
ApoI r'AATT_y

Cuts at:   1493    2107    2614    2840    2992    5101    1493 
   Size:        614     507     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697     614     507     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    3859     160 
   Size:        872    2827    1606

  Fragments arranged by size: 

                2827    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5305
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2630    1870 
   Size:         73     687    4545

  Fragments arranged by size: 

                4545     687      73
 
BamHI G'GATC_C

Cuts at:   2635    3002    2635 
   Size:        367    4938
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2626    4081     618 
   Size:        325     217     627     839    1455    1842

  Fragments arranged by size: 

                1842    1455     839     627     325     217
 
BanII G_rGCy'C

Cuts at:   1391    2624    1391 
   Size:       1233    4072
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    2547     928 
   Size:        971     648    3686

  Fragments arranged by size: 

                3686     971     648
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    3496    3530    1903 
   Size:         34    1559      34    3678

  Fragments arranged by size: 

                3678    1559      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3197    4407    3197 
   Size:       1210    4095
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       5305
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    3916     136 
   Size:        107     122      71     631     605    2244    1525

  Fragments arranged by size: 

                2244    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   2092    5300    2092 
   Size:       3208    2097
 
BlpI GC'TnA_GC

Cuts at:   2722    2722 
   Size:       5305
 
Bme1580I G_kGCm'C

Cuts at:   1790    2452    3366    4522    1790 
   Size:        662     914    1156    2573

  Fragments arranged by size: 

                2573    1156     914     662
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       5305
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    3036    3994     442 
   Size:        416      53      83     399      24    1619     958    1753

  Fragments arranged by size: 

                1753    1619     958     416     399      83      53      24
            
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5305
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2127    2484    3965    1239 
   Size:        888     357    1481    2579

  Fragments arranged by size: 

                2579    1481     888     357
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   3430    4295    4593    3430 
   Size:        865     298    4142

  Fragments arranged by size: 

                4142     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    3968     882 
   Size:        617    2469    2219

  Fragments arranged by size: 

                2469    2219     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    3096     493 
   Size:        711    1892    2702

  Fragments arranged by size: 

                2702    1892     711
 
BsaBI GATnn'nnATC

Cuts at:   5299    5299 
   Size:       5305
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    3112    3494    4129     275 
   Size:         53      83     186     936    1579     382     635    1451

  Fragments arranged by size: 

                1579    1451     936     635     382     186      83      53
            
 
BsaWI w'CCGG_w

Cuts at:   2223    3738    4136    4410    4557    4968    2223 
   Size:       1515     398     274     147     411    2560

  Fragments arranged by size: 

                2560    1515     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    2457    2487    1178 
   Size:         30     165      30    1054      30    3996

  Fragments arranged by size: 

                3996    1054     165      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2078    2139    1186 
   Size:        892      61    4352

  Fragments arranged by size: 

                4352     892      61
 
BseYI C'CCAG_C

Cuts at:   4508    5004    4508 
   Size:        496    4809
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    2042    1163 
   Size:        573     306    4426

  Fragments arranged by size: 

                4426     573     306
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    2755    3516    4544    1540 
   Size:        275     186     754     761    1028    2301

  Fragments arranged by size: 

                2301    1028     761     754     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   2470    3366    3451    4522    2470 
   Size:        896      85    1071    3253

  Fragments arranged by size: 

                3253    1071     896      85
 
BsmI GAATG_Cn'

Cuts at:   2539    2816    2909    2539 
   Size:        277      93    4935

  Fragments arranged by size: 

                4935     277      93
 
BsmBI CGTCTCn'nnnn_

Cuts at:   2060    2060 
   Size:       5305
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2452    2470    2624    3366    3451    4522    1391 
   Size:        399     662      18     154     742      85    1071    2174

  Fragments arranged by size: 

                2174    1071     742     662     399     154      85      18
            
 
BspEI T'CCGG_A

Cuts at:   4968    4968 
   Size:       5305
 
BspHI T'CATG_A

Cuts at:   3194    4924    3194 
   Size:       1730    3575
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2660     844 
   Size:       1816    3489
 
BsrBI CCG'CTC

Cuts at:   1394    1823    3192    1394 
   Size:        429    1369    3507

  Fragments arranged by size: 

                3507    1369     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    2319    2339    3800    3974      65 
   Size:       1183    1071      20    1461     174    1396

  Fragments arranged by size: 

                1461    1396    1183    1071     174      20
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    3949    4136    1700 
   Size:        110    2139     187    2869

  Fragments arranged by size: 

                2869    2139     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       5305
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5305
 
BssSI C'ACGA_G

Cuts at:   3365    4377    3365 
   Size:       1012    4293
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       5305
 
BstBI TT'CG_AA

Cuts at:   4109    4109 
   Size:       5305
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       5305
 
BstYI r'GATC_y

Cuts at:   1972    2092    2635    3002    3387    3404    4856    4942    4954 
   Size:        120     543     367     385      17    1452      86      12

Cuts at:   4954    5300    1972 
   Size:        346    1977

  Fragments arranged by size: 

                1977    1452     543     385     367     346     120      86
                  17      12
 
Bsu36I CC'TnA_GG

Cuts at:   2217    2217 
   Size:       5305
 
BtgI C'CryG_G

Cuts at:    513    1065    2004     513 
   Size:        552     939    3814

  Fragments arranged by size: 

                3814     939     552
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226    1999     487 
   Size:        683      56     773    3793

  Fragments arranged by size: 

                3793     773     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2821    3615    3635    2821 
   Size:        794      20    4491

  Fragments arranged by size: 

                4491     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5305
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5270
 
DraI TTT'AAA

Cuts at:   2609    2609 
   Size:       5305
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4312     809 
   Size:        507    2996    1802

  Fragments arranged by size: 

                2996    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2007    2752    3641 
   Size:         54    1425      50     275      18     177     745     889

Cuts at:   3641    5270       8 
   Size:       1629      43

  Fragments arranged by size: 

                1629    1425     889     745     275     177      54      50
                  43      18
 
EagI C'GGCC_G

Cuts at:   1537    1812    2752    1537 
   Size:        275     940    4090

  Fragments arranged by size: 

                4090     940     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2051    3235    1353 
   Size:        698    1184    3423

  Fragments arranged by size: 

                3423    1184     698
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    3892    4266    4412     387 
   Size:       1064     138    2303     374     146    1280

  Fragments arranged by size: 

                2303    1280    1064     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2075    2127    2484    3368    3965    4752    5020 
   Size:        393     443      52     357     884     597     787     268

Cuts at:   5020    1239 
   Size:       1524

  Fragments arranged by size: 

                1524     884     787     597     443     393     357     268
                  52
 
EcoO109I rG'GnC_Cy

Cuts at:   2138    2138 
   Size:       5305
 
EcoRI G'AATT_C

Cuts at:   2614    2992    2614 
   Size:        378    4927
 
EcoRV GAT'ATC

Cuts at:   2287    2287 
   Size:       5305
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2111    2111 
   Size:       5305
 
FspI TGC'GCA

Cuts at:   3811    3811 
   Size:       5305
 
HaeII r_GCGC'y

Cuts at:   1678    4452    1678 
   Size:       2774    2531
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2062    2094    3946    3978    4020 
   Size:         32     135      32     490      32    1852      32      42

Cuts at:   4020    4052    1373 
   Size:         32    2626

  Fragments arranged by size: 

                2626    1852     490     135      42      32      32      32
                  32      32
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2649    2895     669 
   Size:        988     281     711     246    3079

  Fragments arranged by size: 

                3079     988     711     281     246
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       5305
 
HpaI GTT'AAC

Cuts at:   2895    2895 
   Size:       5305
 
KpnI G_GTAC'C

Cuts at:   2630    2630 
   Size:       5305
 
MfeI C'AATT_G

Cuts at:   2904    2904 
   Size:       5305
 
MluI A'CGCG_T

Cuts at:   5181    5181 
   Size:       5305
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    4419    4603    1305 
   Size:       3114     184    2007

  Fragments arranged by size: 

                3114    2007     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832    2009      10 
   Size:         54    1425     343     177    3306

  Fragments arranged by size: 

                3306    1425     343     177      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2018    2457    3263    3622    3781     518 
   Size:        648     852     439     806     359     159    2042

  Fragments arranged by size: 

                2042     852     806     648     439     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    2309    2360    2721    3398    4546    4791    1606 
   Size:        703      51     361     677    1148     245    2120

  Fragments arranged by size: 

                2120    1148     703     677     361     245      51
 
NcoI C'CATG_G

Cuts at:    513    1065    2004     513 
   Size:        552     939    3814

  Fragments arranged by size: 

                3814     939     552
 
NdeI CA'TA_TG

Cuts at:    387    2155     387 
   Size:       1768    3537
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5305
 
NotI GC'GGCC_GC

Cuts at:   2752    2752 
   Size:       5305
 
NspI r_CATG'y

Cuts at:   1132    1229    2456    2663    4208    1132 
   Size:         97    1227     207    1545    2229

  Fragments arranged by size: 

                2229    1545    1227     207      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       5305
 
PciI A'CATG_T

Cuts at:   2452    4204    2452 
   Size:       1752    3553
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    1108 
   Size:       5305
 
PmeI GTTT'AAAC

Cuts at:   2609    2609 
   Size:       5305
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3374    3406    4887    4919    3374 
   Size:         32    1481      32    3760

  Fragments arranged by size: 

                3760    1481      32      32
 
PpuMI rG'GwC_Cy

Cuts at:   2138    2138 
   Size:       5305
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5305
 
PsiI TTA'TAA

Cuts at:   2875    2875 
   Size:       5305
 
PstI C_TGCA'G

Cuts at:    830    2365    2657     830 
   Size:       1535     292    3478

  Fragments arranged by size: 

                3478    1535     292
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5305
 
PvuII CAG'CTG

Cuts at:   2309    2360    2309 
   Size:         51    5254
 
SalI G'TCGA_C

Cuts at:   1936    2647    1936 
   Size:        711    4594
 
SapI GCTCTTCn'nnn_

Cuts at:   2051    2051 
   Size:       5305
 
SbfI CC_TGCA'GG

Cuts at:   2657    2657 
   Size:       5305
 
ScaI AGT'ACT

Cuts at:   3553    5265    3553 
   Size:       1712    3593
 
SfcI C'TryA_G

Cuts at:    826    1045    2361    2388    2653    3788    4469    4660     826 
   Size:        219    1316      27     265    1135     681     191    1471

  Fragments arranged by size: 

                1471    1316    1135     681     265     219     191      27
            
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5305
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5305
 
SmaI CCC'GGG

Cuts at:   1872    2632    1872 
   Size:        760    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    3409    4310    4572     820 
   Size:        197     926    1466     901     262    1553

  Fragments arranged by size: 

                1553    1466     926     901     262     197
 
SnaBI TAC'GTA

Cuts at:    493    3096     493 
   Size:       2603    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5305
 
SphI G_CATG'C

Cuts at:   1132    2663    1132 
   Size:       1531    3774
 
SspI AAT'ATT

Cuts at:      5      52    2254    3229       5 
   Size:         47    2202     975    2081

  Fragments arranged by size: 

                2202    2081     975      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       5305
 
StyI C'CwwG_G

Cuts at:    513    1065    2004    2744     513 
   Size:        552     939     740    3074

  Fragments arranged by size: 

                3074     939     740     552
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    3332    3349    3502    3687    4132     325 
   Size:       1255    1752      17     153     185     445    1498

  Fragments arranged by size: 

                1752    1498    1255     445     185     153      17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    2403    3551    5263 
   Size:        274      80      33      51    1443     426    1148    1712

Cuts at:   5263      96 
   Size:        138

  Fragments arranged by size: 

                1712    1443    1148     426     274     138      80      51
                  33
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   5265    5297    5265 
   Size:         32    5273
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       5305
 
XbaI T'CTAG_A

Cuts at:   2641    2641 
   Size:       5305
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5305
 
XmaI C'CCGG_G

Cuts at:   1870    2630    1870 
   Size:        760    4545
 
XmnI GAAnn'nnTTC

Cuts at:   2592    3434    2592 
   Size:        842    4463
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    3113     276 
   Size:         53      83     186     936    1579    2468

  Fragments arranged by size: 

                2468    1579     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI    AsiSI
     AvaI    BamHI     BanI    BanII     BbsI     BcgI    BciVI     BclI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BpmI
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstYI   Bsu36I     BtgI    BtgZI     BtsI
     ClaI    CspCI     DraI     DrdI     EaeI     EagI     EarI     EciI
  Eco57MI EcoO109I    EcoRI    EcoRV     FalI     FspI    HaeII    Hin4I
   HincII  HindIII     HpaI     KpnI     MfeI     MluI     MmeI     MscI
     MslI   MspA1I     NcoI     NdeI     NheI     NotI     NspI     PasI
     PciI    PflMI     PmeI     PpiI    PpuMI    PshAI     PsiI     PstI
     PvuI    PvuII     SalI     SapI     SbfI     ScaI     SfcI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SspI     StuI
     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XhoI     XmaI
     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     AloI     ApaI     AscI    AvrII     BaeI     BbeI
    BbvCI    BfrBI     BplI   Bpu10I    BsiWI    BstXI  BstZ17I   DraIII
  EcoICRI    EcoNI     FseI    FspAI     KasI     NaeI     NarI   NgoMIV
     NruI     NsiI     PacI     PfoI     PmlI   PspOMI    PspXI     PsrI
    RsrII     SacI    SacII    SanDI    SexAI     SfiI     SfoI     SrfI
     SwaI     XcmI
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